• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.311-315
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• 2012

A novel ${\beta}$-proteobacterium, designated BXN5-$27^T$, was isolated from soil of a ginseng field of Baekdu Mountain in China, and was characterized using a polyphasic approach. The strain was Gram-staining-negative, aerobic, motile, non-spore-forming, and rod shaped. Strain BXN5-$27^T$ exhibited ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to compound Rd. Phylogenetic analysis based on 16S rRNA gene sequences showed that this strain belonged to the family Comamonadaceae; it was most closely related to Ramlibacter henchirensis $TMB834^T$ and Ramlibacter tataouinensis$TTB310^T$ (96.4% and 96.3% similarity, respectively). The G+C content of the genomic DNA was 68.1%. The major menaquinone was Q-8. The major fatty acids were $C_{16:0}$, summed feature 4 (comprising $C_{16:1}$ ${\omega}7c$ and/or iso-$C_{15:0}$ 2OH), and $C_{17:0}$ cyclo. Genomic and chemotaxonomic data supported the affiliation of strain BXN5-$27^T$ to the genus Ramlibacter. However, physiological and biochemical tests differentiated it phenotypically from the other established species of Ramlibacter. Therefore, the isolate represents a novel species, for which the name Ramlibacter ginsenosidimutans sp. nov. is proposed, with the type strain being BXN5-$27^T$ (=DSM $23480^T$ = LMG $24525^T$ = KCTC $22276^T$).

• Journal of the Korean Institute of Intelligent Systems
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• v.19 no.6
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• pp.865-871
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• 2009

In this paper, a novel feedback linearization is proposed for discrete-time nonlinear systems described by discrete-time T-S fuzzy models. The local linear models of a T-S fuzzy model are transformed to a controllable canonical form respectively, and their T-S fuzzy combination results in a feedback linearizable Tagaki-Sugeno fuzzy model. Based on this model, a nonlinear state feedback linearizing input is determined. Nonlinear state transformation is inferred from the linear state transformations for the controllable canonical forms. The proposed method of this paper is more intuitive and easier to understand mathematically compared to the well-known feedback linearization technique which requires a profound mathematical background. The feedback linearizable condition of this paper is also weakened compared to the conventional feedback linearization. This means that larger class of nonlinear systems is linearizable compared to the case of classical linearization.

• Korean Journal of Construction Engineering and Management
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• v.19 no.4
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• pp.82-92
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• 2018

Considering that earlier stage of construction project has a great influence on the possibility of lowering of environmental load, it is important to build and utilize system that can support effective decision making at the initial stage of the project. In this study, we constructed an environmental load estimation model that can be used at the early stage of the project using basic design factors. The model was constructed by using the artificial neural network to estimate environmental load by applying to planning stage (ANN-1), basic design stage (ANN-2). The result of test, shows that average of absolute measuring efficiency and standard deviation of ANN-1 and ANN-2 were 11.19% / 5.30% and 9.59% / 3.09% each. This result indicates that the model using the input variables extended with the project progress has high reliability and it is considered to be effective in decision support at the initial design stage of the project.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.49 no.3
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• pp.67-73
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• 2012

Local color transfer is the process of assigning a given color to a local region in a target image. The local region that contains the given color has to be segmented. Conventionally, the segmentation of the corresponding local region in a target image is based on the color distance. The region which is the closest in color distance is separated. However, since the close range of color distance separating a given color from target image is ambiguous and uncertain, color distortion is often generated around a separated local region. Therefore, this paper addresses the problem of segmentation in a local color transfer. To prevent color distortion, a modified color influence map is proposed with color categories. First, the target image is roughly segmented using a color category map, which groups similar colors in color space. It restricts the color transfer to a specific color category according to a given color. Second, modified color influence map assigning different weight to lightness and chroma, respectively, is used instead of Euclidian color distance. Then, by combining a modified color influence map and color category map filtered with anisotropic diffusion, a local region that contains a given color can be segmented more strictly than conventional method.

• The Journal of Korean Academy of Sensory Integration
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• v.9 no.1
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• pp.21-31
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• 2011

Objective : This study investigated the relationships of handwriting legibility and perceptual-motor skills, and handwriting speed and perceptual-motor skills. And identified the predictors that most affect the handwriting of preschool children. Methods : Twenty-three typically developing preschool aged children (mean age: 68.61 months, SD=2.04) were selected through the Korean-Denver Developmental Screening Test-2(K-DDST-2). The children were tested with regard to handwriting legibility, visual perception, visual-motor integration and fine-motor coordination. Results : First, a significant relationship was not found among handwriting legibility, visual perception, visualmotor integration and fine-motor coordination. Second, a significant relationship was found among handwriting speed, visual perception and fine-motor coordination. Third, stepwise multiple regression analyses showed that general visual perception were significant predictors for handwriting speed. Conclusion : Occupational therapists should evaluate children's visual perception levels utilizing a standardized test, and focus on general visual perception in order to improve handwriting skill(speed). Also, occupational therapists are expected to play an important role in the management and treatment of children's handwriting skills.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.11 no.4
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• pp.103-112
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• 1991

In this study, a numerical model was established and applied to simulate the steady-state groundwater and heat flow in an isotropic, heterogeneous, three dimensional aquifer system with uniform thermal properties and no change of state. This model was developed as an aid in screening large groundwater-flow systems as prospects for underground waste storage. Driving forces on the system are external hydrologic conditions of recharge from precipitation and fixed hydraulic head boundaries. Heat flux includes geothermal heat-flow, conduction to the land surface, advection from recharge, and advection to or from fixed-head boundaries. The model uses an iterative procedure that alternately solves the groundwater-flow and heat-flow equations, updating advective flux after solution of the groundwater-flow equation, and updating hydraulic conductivity after solution of the heat-flow equation. Dierect solution is used for each equation. Travel time is determined by particle tracking through the modeled space. Velocities within blocks are linear interpolations of velocities at block faces. Applying this model to the groundwater-flow system located in Jigyung-ri. Songla-myun, Youngil-gun. Kyungsangbuk-do, the groundwater-flow system including distribution of head, temperature and travel time and flow line, is analyzed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.2
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• pp.93-98
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• 2015

This study was conducted to investigate the effect of different stockpiled periods on the nutritive value and productivity of mixed orchardgrass tall-fescue pasture (MOTP). This experiment was conducted at Jeju (island), South Korea, from Sep. 2012 to Nov. 2012. The DM yield of the MOTP had the highest in treatment in late August (2,983 kg/ha). The DM yields of swards were increased significantly by shortening the stockpiled period. The CP of the MOTP was 16.5% to 18.16%, and there did not appear to be a consistent trend in accordance with the duration of the stockpiled periods. The NDF and TDN content of the MOTP increased with the delay of the stockpiled periods, but there is a significant difference between the short- and long-stockpile periods; however, the ADF content of the MOTP decreased with shortened stockpile periods, but again, there is a significant difference between the two periods. In addition, P, Ca, Mg, Na, and Zn of MOTP increased by delaying the stockpile period, but Mn and Cu of MOTP decreased. As shown in the results of this research, the yield of MOTP was not increased by the atrophy of the growth of MOTP due to high temperatures in case where the MOTP was stockpiled from the beginning of August. The stockpile period of MOTP should therefore be started at the end of August to yield a stockpile of MOTP in the autumn.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.19-25
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• 2006

With the use of Agrobacterium and gene-gun, cold regulated gene (BN115) has been injected in Chrysanthemum leaf disc and transgenic plants have been produced successfully on the selection media containing phytohormone. To determine the presence of the transferred cold regulated gene (BN115) in the transgenic Chrysanthemum, PCR-amplification indicated the presence of that gene. Real-Time PCR for confirmation of the putative transgenic plants was established. The copy number of cold regulated gene (BN115) is extrapolated on the basis of a standard curve. Serial dilutions of known number of gene copies were in triplicates. In this diagram, PCR cycles are plotted against the fluorescence intensity. The cycle at which the fluorescence reaches a threshold cycle is inversely proportional to the starting amount of target DNA.

• Journal of Acupuncture Research
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• v.29 no.1
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• pp.103-113
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• 2012

Objectives : The purpose of this study is to evaluate effect of suppressing the expression of cyclo-oxygenase-type-2 (COX-2) as a consequence of inhibition macrophage migration inhibitory factor (MIF) activation by $Sambucus$ $williamsii$ $Hance$ (SWH) pharmacopunctureon rheumatoid arthritis (RA). Methods : In vitro test, synoviocytes extracted from type II collagen-induced arthritis (CIA) mouse's knee joint were cultivated After that, each well of synoviocytes was mixed with the extract of SWH at the dosage of $0.4mg/m{\ell}$, $0.6mg/m{\ell}$, $0.8mg/m{\ell}$, and $1.0mg/m{\ell}$ respectively, and cultivated for 24 hours after the addition. Reverse transcriptase - polymerase chain reaction (RT-PCR) is used to investigate the expression of MIF, Tumor necrosis factor (TNF)-${\alpha}$, COX-2 mRNA. $In$ $vivo$ test, thirty DBA female mice were used, and each ten mice were allocated into three group; normal group, CIA-elicitated group, and group treated with SWH pharmacopuncture on it's the point of $ST_{35}$ after CIA elicitation. It is investigated that change of mice foot thickness, histologic change of sliced synovial joint of mouse, and extent change of MIF, TNF-${\alpha}$, COX-2 in synovial membrane. Results : $In$ $vitro$ test, the expressions of cytokine(MIF, TNF-${\alpha}$, COX-2) mRNAs related to RA were dose-dependent decreased. In the SWH pharmacopuncture group, foot thickness and histologic change of sliced synovial joint were decreased comparing with CIA-elicitated group's change. In the SWH pharmacopuncture group, the suppression of MIF, TNF-${\alpha}$, COX-2 in synovial membrane was clearly shown comparing with CIA-elicitated group's change. Conclusions : It might be suggested that SWH pharmacopuncture mitigate tissue damage originated from rheumatoid arthritis by suppressing the expression of COX-2 as a consequence of inhibition MIF activation.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.2
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• pp.141-147
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• 2015

The contrast of a display is generally evaluated by using the ratio of the minimum luminance to the maximum luminance of the display. However, this contrast ratio is not matched with perceived contrast because it uses only physical characteristic of display without considering human perceptual characteristics. In this paper, the proposed contrast measure that considers perceptually discriminable brightness within the range of display brightness is suggested. First, the range between the minimum and maximum brightness of display in CIECAM02 color space is calculated to measure the length of perceived brightness for the display. Next, brightness ranges which are perceptually same at each brightness level are determined by applying Weber-Fechner ratio and then, the number of brightness values within each brightness range is counted. Finally, perceptually discriminable brightness is defined as the sum of ratio between the number of brightness values in each brightness range and the perceptual contrast length of the display. In the experiments, preference test using various displays with random brightness patches is performed to evaluate perceived contrast. As a result, the proposed measure is more consistent with human perception than the previous contrast measures.