• Journal of Applied Biological Chemistry
• /
• v.56 no.3
• /
• pp.147-156
• /
• 2013

The objectives of this study were to investigate the effect of Lactobacillus paracasei subsp. tolerans JG22 isolated from pepper leaf jangajji on the mutagenic activity of N-methyl, N'-nitro, N-nitrosoguanidine (MNNG) and 2-nitrofluene (2-NF) and to evaluate the effect of physico-chemical pretreatment on the antimutagenic activity of the strain. The viable cells of JG22 strain displayed a significantly high (p <0.05) antimutagenic activity against both mutagens tested. The antimutagenic effect of JG22 strain seems to be positively correlated with the amounts of the cells in the incubation time. This strain produced the antimutagenic activity of the maximum levels after preincubation for 30 min. The binding of this strain against the mutagenic compounds might be mainly present in the cell wall fraction rather than the cytosol fraction. Pretreatment with proteolytic enzymes and simulated gastric and intestinal juices and at different pH values had no significant effect on two mutagens removal by the viable cells. However, the binding activity of the mutagen by the strain seems to be affected by heating, enzymes including $\alpha$-amylase and lysozyme, divalent ions, and sodium metaperiodate. Thus, carbohydrates consisting of the cell walls may be important elements responsible for the binding of MNNG and 2-NF by this strain. In conclusion, the binding of the mutagens to cells of JG 22 strain may play a vital role in suppressing the process of mutagenesis induced by mutagens.

• Journal of Embryo Transfer
• /
• v.32 no.2
• /
• pp.47-52
• /
• 2017

As a part of the effort to improve post-transfer survival rate of embryos in Korean black goats, a technique for laparoscopic uterine transfer of blastocysts was carried out. A total of 26 transferrable embryos (morula to expanded blastocysts) were transferred to 13 recipient goats via transabdominal laparoscopic method. In consequence of our hormone protocol, 65% of the recipients (13/20) were found to have synchronized estrus. After confirmation of corpus luteum in each recipient goat, a Babcock laparoscopic forceps was inserted into the lower abdominal cavity to hold a uterine horn and fasten it near the peritoneum without causing injury. Then 7.5cm long 16G IV catheter was inserted directly into the uterine lumen through the abdominal wall. After removal of the stylet of the IV catheter, the embryo transfer tube (identical in size to the stylet and loaded with blastocysts) was inserted into the uterine lumen through the catheter to unload the embryos. Of the 13 estrus synchronized recipients, 9 were transferred blastocysts and 4 were transferred molurae (2 embryos in each recipient) in uterine ipsilateral to the ovary with corpus luteum. Four of the 9 recipients which blastocysts were transferred using this method has been confirmed pregnant (44.4% pregnancy rate).

• Journal of Korean Neurosurgical Society
• /
• v.29 no.9
• /
• pp.1238-1242
• /
• 2000

Spinal schwannomas are usually extramedullary intradural tumors and their intramedullary localizations are thought to be extremely rare. A 60-year-old woman complaining spastic quadriparesis, voiding difficulty and dyspnea was admitted. Her cervical MRI revealed an intramedullary mass in the cervicomedullary junction with multiple cyst which extended from lower cervical to C3 spinal cord. The mass showed a low signal on T1WI, high signal on T2WI with an wall enhancement. The patient underwent a suboccipital craniectomy and C1-2 laminectomy and the cystic tumor was totally removed through a midline myelotomy. The tumor was proved as an intramedullary schwannoma by pathologic examination. The Intramedullary presence of a tumor arising from the cells of the nerve sheath is unusual, because the central nervous system fibers do not contain the Schwann cell. There have been several hypotheses, but none has been accepted universally. This rare tumor is considered as a curable benign neoplasm, and an accurate intraoperative diagnosis and surgically total removal are essential.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.23 no.4
• /
• pp.376-379
• /
• 2001

There are many causes of trismus. Aetiology can be roughly divided into muscle spasm, mechanical interference, extracapsular ankylosis, intracapsular ankylosis. Trismus caused by mechanical interference between postero-lateral wall of zygoma-maxillary complex and coronoid process following reduction of fractured facial bone is rare. Especially on maxillary bone fracture, when we faced the trismus following removal of intermaxillary fixation, we got used to solve that problem by physical exercise. We obtained good results by coronoidectomy on patients with limited mouth opening who were referred from department of plastic surgery, St. Marys' hospital, the Catholic university of Korea. We report our experience with literature review.

• Geotechnical Engineering
• /
• v.7 no.2
• /
• pp.27-40
• /
• 1991

The Sunghak Campus, Dong-A University, which located at the excavated area of mountain, has been constructed year by year for about 10 years since 1978. The buildings, steep slopes, road and so on in the Campus area were damaged by heavy rainfall more than 200mm 1 day on August, 1989. The methods used for the investigation and repair methods of the damage are the preliminary investigation, the present condition survey, 50 by 50m grid survey, compasstraverse for locating outcrops, geopysical exploration, geologic survey, boring and laboratory soil testing. Based on the results of investigations, the causes of the failures have been evaluated, and the repair methods have been set up as horizontal drains, removal of the dangerous rock, retaining wall with subsurface drains, the elimination of colluvium and slope stabilization by use of precast concrete grids and so on.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2006.03a
• /
• pp.1014-1023
• /
• 2006

Soil nailing is a reinforcement method used for stabilizing excavated wall or slope. Due to its many advantages such as ease of construction and economical efficiency, use of soil nailing is increased. However, the soil nail can't trespass on the neighbor private land, which pays rent for use. For this reason, removable soil nailing system was developed. However, the removal rate of this system is just about $50\sim70%$. To solve this, the Fiber Reinforced Polymer (FRP) soil nailing system, which does not need to be removed and allows for the trespass on the private land, is developed. In this paper, through theoretical and experimental studies in laboratory and field, we evaluate the stability and behavior characteristics of the FRP nail system. Besides, numerical analyses using FLAC2D were performed for various soil conditions, where the simulations for pullout tests were carried out. As a result, compared with the conventional removable soil nailing system, the FRP soil nailing systems show similar behavior characteristics.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2005.03a
• /
• pp.1496-1503
• /
• 2005

The study on the lateral earth pressure is briskly preformed for various conditions such as type of retaining walls, ground condition, and type of supporting systems. It is not simple to determine the distribution of lateral earth pressure accurately, however, because the lateral earth pressure is affected by various factors. This study is performed to analyze the behavior of earth retaining walls for new excavation contacting with existing excavation by comparing with the site measuring values before and after new excavation. On the base of observation, the distribution of strut axial forces is similar to that of ganeral earth retaining walls, but strut axial forces is increased by removal of existing earth anchors. When new excavation is performed contacting with existing excavation, the axial force of strut is decreased because of soil exclusion in the behind walls, but that force is increased after new exeavation. The analysis result show that the installation of strut in middle part makes a effect to not only 1 adjacent strut, but 3-5 adjacent struts. Also during new excavation strut axial forces is decreased by relaxation of total earth retaining wall system.

• Advances in pediatric surgery
• /
• v.15 no.1
• /
• pp.68-72
• /
• 2009

Neuroblastoma is the most common extracranial solid tumor in children, and accompanies various clinical symptoms including hypertension. Hypertension is associated with catecholamines secreted from the tumor, and is usually not severe. We report one case of malignant hypertension with cardiac failure in a patient with adrenal neuroblastoma, successfully treated with adrenalectomy. A 3 year-old boy complained of protrusion of the chest wall. Physical examination revealed severe hypertension with cardiac failure. The levels of metabolites of catecholamine were increased in blood (norepinephrine >2000 pg/mL) and urine (norepinephrine 1350.5 ug/day). Abdominal CT showed a 7 cm-sized solid mass arising from the right adrenal gland. After stabilizing the hemodynamics with oral phenoxybenzamine, right adrenalectomy was performed. Pathological diagnosis was a ganglioneuroblastoma. The hypertension and cardiac failure were resolved after tumor removal.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.7 no.1
• /
• pp.74-77
• /
• 2004

Anisakiasis is a accidental parasitic infection caused by nematode larvae belonging to the subfamily Anisakinae when a raw or inadequately cooked fish is ingested. The common clinical symptoms are severe colicky abdominal pain or epigastric full sensation, nausea, vomiting and fever, but hematemesis or melena is very rare. We report a case of a 11-year-old female child who developed severe epigastric pain recurrently for 2 months, and recalled that she had eaten the raw flesh of an Astroconger myriaster. Endoscopic examination showed the whitish worm invading the stomach wall. Clinical symptoms disappeared after endoscopic removal. This study may be the first pediatric case of gastric anisakiasis in korea.

• Molecules and Cells
• /
• v.26 no.6
• /
• pp.554-557
• /
• 2008

Double-stranded RNA (dsRNA) induces gene silencing in a sequence-specific manner by a process known as RNA interference (RNAi). The RNA-induced silencing complex (RISC) is a multi-subunit ribonucleoprotein complex that plays a key role in RNAi. VIG (Vasa intronic gene) has been identified as a component of Drosophila RISC; however, the role VIG plays in regulating RNAi is poorly understood. Here, we examined the spatial and temporal expression patterns of VIG-1, the C. elegans ortholog of Drosophila VIG, using a vig-1::gfp fusion construct. This construct contains the 908-bp region immediately upstream of vig-1 gene translation initiation site. Analysis by confocal microscopy demonstrated GFP-VIG-1 expression in a number of tissues including the pharynx, body wall muscle, hypodermis, intestine, reproductive system, and nervous system at the larval and adult stages. Furthermore, western blot analysis showed that VIG-1 is present in each developmental stage examined. To investigate regulatory sequences for vig-1 gene expression, we generated constructs containing deletions in the upstream region. It was determined that the GFP expression pattern of a deletion construct (${\Delta}-908$ to -597) was generally similar to that of the non-deletion construct. In contrast, removal of a larger segment (${\Delta}-908$ to -191) resulted in the loss of GFP expression in most cell types. Collectively, these results indicate that the 406-bp upstream region (-596 to -191) contains essential regulatory sequences required for VIG-1 expression.