This study was carried out to find out how space allowance affect the social behavior of Korean native cattle (Bos taurus coreanae) steers. Twelve Korean native cattle (Bos taurus coreanae) steers were used as subjects, each of which was 30-month-old and observation period was from June to July 2003. Five (T1) and seven (T2) steers were allotted to two pens of $5m{\times}10m$ in a building with slate roof and open sides respectively. They were fed at 09:00 h and 16:00 h, twice a day. The behaviors of steers were recorded from 06:00 h to 17:00 h, using two color CCD cameras (Samsung SDC-411, Korea), one B/W CCD cameras (Samsung SBC-340, Korea), one multiplexer (Samsung SDM-081, Korea) and a time lapse VCR (Samsung SRV-30, Korea). The behaviors of each steer were recorded every 2 min using an instantaneous point sampling method. While the mean percentage of time budget in WA of T1 was lower than that of T2 (p<0.05), the mean percentage of time budget in SF of T1 was higher than that of T2 (p<0.05). When it gets hot, steers in T1 rested from 10:00 h to 14:00 h when it gets cool, showing 40~80% of LD rate while steers in T2 rested from 12:00 h, when it very hot to 17:00 h, showing 20~50% of LD rate, which is relatively low. Steers in T1 were fed from 06:00 h to 08:00 h when it was cool and from 16:00 h to 18:00 h, showing 20~45% of EA rate while steers in T2 were fed from 08:00 h to 14:00 h when it was hot, showing 25~50% of EA rate. In conclusion, it turned out that the number of steers affected their social behavior, and T1 was better environment than T2 in terms of welfare.
When alginate lyase gene (aly) from Pseudoalteromonas elyakovii was expressed in E. coli, most of the gene product was produced as aggregated insoluble particles known as inclusion bodies. In order to produce a soluble and active form of alginate lyase, E. coli cells fore cotransformed with the plasmids designed to permit coexpression of aly together with molecular chaperones such as DnaK/DnaJ/GrpE or GroEL/ES chaperones. The results revealed that the coexpression of aly together with DnaK/DnaJ/GrpE chaperone had a marked effect on the production of this protein as a soluble and active form, presumably through facilitating correct folding of alginate lyase protein. The optimal concentration of L-arabinose for the induction of DnaK/DnaJ/GrpE chaperone was found to be 0.05 mg/ml. When DnaK/DnaJ/GrpE chaperone was coexpressed, about 34% in the total alginate lyase was produced in the soluble fraction. By addition of 10% cetylpyridinium chloride, a clear zone around the colony coexpressing aly and DnaK/DnaJ/GrpE chaperone was formed, indicating that the alginate in the medium was hydrolyzed by active alginate lyase enzyme.
Antitumouric effect of loquat was investigated treating the extract from leaves, stems, fruits or seeds to normal and cancer cell lines to check by MTT method wheather the cancer cells are specifially attacked. The results are summarized as follows. Water of MeOH extract from each organ were applied to human normal and cancer cell lines, SNU-1 and SNU-C4. The water extract from fruit flesh gave no dffect to normal cell lines by killed all the cancer cell lines. The water extract from fruit flesh was purified by Sephadex LH-20 and separated into 9 fractions which were than applied to 8 cancer cell lines. The eighth fraction out of the 9 fractions gave no effect to normal cells but exerted specific cytotoxicity to breast cancer, stomach and liver cancer cells. The eighth fraction was orally administed and injected to 10 mice each suffering from the abdominal cancer induced by myeloma cells, SP2/0-Ag14. In the groups received the treatment, only one mouse each died in 2 months but the rest survived until the end of the experimental period, which those in the control plot died in 10 to 13 days. The present results confirmed that loquat contained some substance that had specific cytotoxicity to human cancer cells.
Kim, Kyoung-Duck;Seo, Joo-Young;Hong, Su-Hee;Kim, Jeong-Ho;Byun, Hee-Guk;Kim, Kang-Woong;Son, Maeng-Hyun;Lee, Sang-Min
Korean Journal of Fisheries and Aquatic Sciences
/
제44권2호
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pp.141-148
/
2011
This feeding experiment was conducted to investigate the effects of dietary inclusion of various additives on growth performance, hematological parameters, fatty acid composition, gene expression and histopathological changes in juvenile olive flounder (Paralichthys olivaceus). Eleven isonitrogenous (49% crude protein) and isolipidic (10% crude lipid) experimental diets were formulated: no additives (Con); 5% kelp meal (Ke); 10% krill meal (Kr); 1% garlic powder (Ga); 1% citrus meal (Ci); 3% onion powder (On); 1% ginger powder (Gi); 1% mugwort powder (Mu); 1% licorice powder (Li); 1% wasabi powder (Wa); and a mixture (Mix) of these additives. Three replicate groups of juvenile flounder (average weight of 8.5 g) were fed one of the experimental diets to visual satiety twice a day for 15 weeks. The dietary inclusion of additives did not affect survival, weight gain, specific growth rate feed efficiency, daily feed intake, daily protein intake, protein efficiency ratio, hepatosomatic index and visceralsomatic index of the fish. Plasma triglyceride levels were significantly lower in fish fed the Ke, Ga, On, Gi, Mu, Li, and Mix diets than in fish fed the control diet. Plasma glucose, glutamic oxaloacetic transaminase and total cholesterol did not differ among dietary treatments. No significant difference was observed in fatty acid composition and lipid content of the dorsal muscle in fish fed the experimental diets. Myosin gene expression did not differ significantly among treatments after 5 weeks but was significantly lower in fish fed the Kr, Ci, Li, and Mix diets than in control group after 15 weeks. Histopathological analysis showed mild gill hyperplasia and mild necrosis of liver parenchymal cells in several individuals of each experimental group. These conditions were also observed in the control group and were not thought to be related to the inclusion of feed additives. The present findings indicate that the dietary inclusion of additives did not affect growth performance, fatty acid composition, gene expression, and histopathological changes in juvenile flounder. However, plasma triglyceride content may be reduced by supplementation with 5% kelp meal, 3% onion powder, 1% garlic powder, 1% ginger powder, 1% mugwort powder, and the additive mixture.
Proceedings of the Korean Institute of Information and Commucation Sciences Conference
/
한국정보통신학회 2018년도 추계학술대회
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pp.522-525
/
2018
In this paper, we design a UWB / WAS microstrip antenna for wireless local high - speed communication. The substrate of the proposed antenna is FR-4 (er = 4.3) and its size is $30[mm]{\times}30[mm]$. It is designed to have characteristics that can be used in the frequency bands of 3.5 [GHz] and 5.2 [GHz], which are UWB / WAS frequency bands. The simulation was performed using CST Microwave Studio 2014. Simulation results show that the gain is 1.592 [dBi] at 3.5 [GHz] and 2.210 [dBi] at 5.2 [GHz]. The S-parameter also showed a result of less than -10 [dB] (WSWR 2: 1) in the desired frequency band. Microstrip antennas have been miniaturized, high performance, and light weight, and excellent and low cost systems are continuously being developed. In addition, many people use wireless local area network systems used in homes, companies, and public facilities. Since the UWB / WAS technology is proposed according to the development of the system and the demand increase, the antenna that satisfies the above conditions will be designed and the technology applicable to the system will be used more conveniently.
Gradient concentrations of Tithonia diversifolia green leaves and phosphate rocks were used to investigate their contributions as a fertilizer to the yield and quality improvement of a rainfed rice cultivar. Six treatments were compared: (1) T0, no fertilization (control); (2) T1, 1.28 g of phosphate rocks; (3) T2, 250 g of organic matter; (4) T3, 500 g of organic matter; (5) T4, 250 g of organic matter + 1.28 g of phosphate rocks; (6) T5, 500 g of organic matter + 1.28 g of phosphate rocks. The results showed that the germination percentage recorded 15 days after sowing varied from 58 - 76% between T0 and T5. The number of panicles ranged between 2 (T0) to 6.3 (T5). Moreover, the recorded length of the panicles ranged between 7.5 (T1) to 15.8 cm (T2), and the number of grains per panicle ranged between 25.5 (T1) to 273.5 (T3). The plant height was significantly increased in the T5 (79.27 cm) group compared to the T1 (33.63 cm) and control treatment (T0) (40.08 cm) groups. Although the plant height in the T2, T3, and T4 groups was slightly lower than the T5 group, the difference was not statistically significant. The average of the grain number per plant was high in the T3 (273.6 grains) group compared to the T1 and T0 (25.5 and 32.8 grains) groups, respectively. These results suggest that the combination of T. diversifolia leaves and phosphate rocks as a natural fertilizer would be beneficial when integrated into soil fertility management strategies and would contribute to improving crop yield and quality.
Choi Min Joo;Lee Jong Soo;Kang Gwan Suk;Paeng Dong Guk;Lee Yoon Joon;Cho Chu Hyun;Rim Geun Hie
The Journal of the Acoustical Society of Korea
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제24권5호
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pp.271-281
/
2005
An electromagnetic type shock wave generator suitable for extracorporeal shock wave therapy has been constructed by employing a solenoid coil. The Property of the shock waves produced by the shock wave generator was evaluated using a needle hydrophone. It was shown that, as the capacitor discharging voltage increased from 8 to 18 kV, the Positive Peak Pressure (P+) of the shock wave increased non-linearly from 10 to 77 Wa. In contrast. the negative peak Pressure (f) varied between -3.2 and -6.8 MPa. had its absolute maximum of -6.9 ma at 14 kV The peak amplitudes P+ measured repeatedly under the same voltage setting varied within $5\;\%$ from mean values and this is very small compared to about $50\;\%$ for electrohydraulic type shock wave generators. It could be observed, from the hydrophone signal recorded over 1 ms. several sequential acoustic impulses representing bubble collapses. namely. acoustic cavitation. induced by the shock wave. A technique based on wavelet transformation was used to accurately measure the time delay between the 1st and 2nd collapse known to be closely related to the shock strength. It was observed that the measured time delay increased almost linearly from 120 to $700\;{\mu}s$ with the shock wave Pressure P+ increasing from 10 to 77 MPa.
In this study we have calculated an ideal complex refractive index of a TiN trim used in a layer of anl1reilecnon (I\R) coatmg, [air$ISiO_2ITiNIglass$] in the visible. Also we simulated the rellectance of lwo-layer AR coating by varying the thicknesses of TiN and $SiO_2$ layers, respecl1vely. The simolation results show that we can controllhe lowest reflectance and AR band of tile AR coating. The TIN fihns were fabricated by a RF magnetron sputtering apparalus. The chemical, structural and electrical properties of TiN fih11S were inveshgated by the Rutherford backscattering spech'oscopy (RBS), atomic force microscope (AFM) and 4-point probe. The optical properlies were inve,tigated by the spectrophotometer and vanable angle spectroscopic ellipsometer (VASE). The smface roughness of TiN flhns \vas $9~10\AA$. TIle resistivity of TiN films was TEX>$360~730\mu$\Omega $ cm. The ,toichlOllletry of TiN film was 1'1: O:N = I: 0.65 :0.95 and ilic oxygen wa~ found on ilie smface. With these experimental and simu]al1on resulLs, we deposited duo: two-layer AR coating, [air$ISiO_2ITiNIglass$] and the refleClance was under 0.5% ill the regIOn of 440-650 run. 0 run.
The shade of restorative materials is very important for successful restoration esthetically harmonized with the natural tooth. The purpose of this study was to evaluate the color stability and the opacity change of conventional chemical setting and visible light curing glass-ionomer cements for restorative esthetic filling. Specimens of each brand (GC Fuji II and Fuji II LC) were uniformly prepared and divided into three groups: In group l(control group), the specimens received no surface treatment; in group 2, the specimens were coated with varnish and the excess gently blown off; and in group 3. the specimens were coated with light-cured bonding resin and irradiated by a visible light curing unit for 20 seconds on both sides. All specimens were stored in distilled water at $37^{\circ}C$ and checked after for 24 hours. 2 months. 4 months, 6 months. and 8 months. The color characteristics($L^*,\;a^*,\;b^*$) and the opacity(Y%) of all the samples were measured by a spectrocolorimeter and the total color differences(${\Delta}E^*$) and opacity changes(${\Delta}Y%$) were computed. The following results were obtained : 1. The total tolor differences in all groups increased with time. 2. The total color differences of the LC groups after immersion for 8 months in distilled water at $37^{\circ}C$ were lower than those of GC groups(p<0.01), and the total color differences of the varnish or the light-cured bonding resin coated groups were lower than those of the control group with glass-ionomer cements which had no surface treatment(p<0.01). 3. In all groups the translucency decreased with time. 4. In the control group and the varnish coating group. the opacity changes of the GC groups were lower than those of the LC groups(p<0.01) and in the light-cured bonding resin coated group, there was no significant difference between the GC group and the LC group. 5. The opacity changes of the varnish or the light-cured bonding resin coated groups were lower than those of the control group(p<0.01). These results suggest that color change and opacity of conventional chemical setting and light cured glass-ionomer cement were increased with time, and the color changes and the opacity changes of a control group after immersion for 8 months in distilled water at $37^{\circ}C$ were greater than those of the varnish or the light-cured bonding resin coated groups.
Few observation have been made on the pericardial pressure and little is known about the composition of he pericardial fluid. So we studied the basic qualitative and quantitative analysis of the pericardial fluid in the patients with cardiac disease either congenital heart diasese(group A) or acquired heart disease(group B). The pressure of the pericardial cavity was measured by the method of open tipped water filled small polyethylene catheter connecting to the standardized monitor, which was introduced into pericardium of the patients who were performed pericardial incision for the heart or pericardial surgery. All of the data was compared to the simultaneously checked hematologic value of the same patient. The mean pressure of the pericardial cavity was 2.4mmHg and the amount of the pericardial fluid was 13cc/m2 of body surface for the group A and 17.7cc for the group B. And the cell count was 138$\pm$l16/1 in group A and 230$\pm$135/1 in group B and the pH was 7.83$\pm$0.40 in group A. 7.80$\pm$0.52 in group B. Pericardial fluid revealed satisfactically significant alkaline pH than plasma. The fundamental electrolyte, Wa+, K+, Cl and glucose were identical to the hematologic values of the same patient, but the protein concentration was 2.Bg/dL for group A and 3.Ig/dL for group B heart disease and those were remarkable low concentration compared to the hematologic value of the same patient. LDH and amylase were identical to the value of the serum of the same patient, but the concentration of LDH of group B was slightly higher than that of the group A.
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