• Proceedings of the PSK Conference
• /
• 2000.10a
• /
• pp.278.1-278.1
• /
• 2000

• Archives of Pharmacal Research
• /
• v.25 no.6
• /
• pp.954-963
• /
• 2002

Lectin-conjugated praecoxin A is a compound, which is combined Wheat Germ Agglutinin (WGA) Lectin with praecoxin A and also known to have an anti-tumor activity. In our lab, in order to investigate its immune reaction other than the anti-tumor activity ever known, we examined cytokines such as IL-6 and IL-12 through their mRNA expressions, which are generally secreted by macrophage both in vivo and in vitro. To analyze, we used RT-PCR for total RNAs of macrophages. As a result, we obtained that both in vitro and in vivo, lectin-conjugated praecoxin A showed an interesting increase on IL-6 and IL-12 even though it may be little hard to say the conjugated form is absolutely more effective than that of lectin or praecoxin A alone for immune response activities. Those results suggest that the conjugated form may give an additional opportunity in a future therapeutic use over its immuno activation properties.

• Proceedings of the KOR-BRONCHOESO Conference
• /
• 1991.06a
• /
• pp.16-16
• /
• 1991

렉틴(Lectins)은 세포막의 당단백질의 과당류 말단기에 특이적으로 결합하는 비면역성의 당단백질 혹은 단백질을 말하는데 정상세포와 변형된 세포에서 렉틴반응의 차이가 남으로써 요즘에는 렉틴을 병리학적 진단에 이용 가능하게 되었다. “Loss of contact inhibition”은 세포 악성화의 중요한 특징으로 세포의 악성화는 세포막의 구조의 변화와 관계가 있을 것으로 알려져 왔으며 1989년 후두암에서 PNA의 반응이 양성 반응을 나타낸다고 보고되어 저자들은 7가지의 렉틴을 이용하여 후두암전구증의 하나인 후두각화증에서 이형성(Atypia)의 존재유무에 따른 렉틴 반응의 변화를 알아보고자 본 연구를 시행하여 다음과 같은 결과를 얻었다. 1. 이형성이 없는 단순 후두각화증에서는 Con A와 WGA만이 기저세포에 반응이 있었으며, 후두각화증의 가장 중요한 부위인 극세포층에서는 WGA, RCA-I, PNA, SBA, UEA-I, DBA가 세포질에는 반응이 없이 세포간교에만 염색이 되는것을 관찰할 수 있었고 Con A는 반대로 극세포층의 세포질에 반응을 하였으나 세포간교에는 반응이 없었다. 2. 이형성을 동반한 후두각화증에서, 기저세포에서는 반응의 차이가 없었고 극세포층에서는 UEA-I, RCA-I. WGA, PNA, SBA는 세포간교에서 반응이 나타나지 않았고 세포질에서 양성 반응을 관찰 할 수 있었으며 DBA, Con-A에서는 반응의 차이를 관찰 할 수 없었다. 따라서 후두각화증의 극세포층 세포막에서 UEA-I, RCA-I, WGA, PNA, SBA 반응의 소실은 후두암으로의 진행과 밀접한 관계가 있는 이형성의 존재를 암시한다하겠다.

• Fisheries and Aquatic Sciences
• /
• v.1 no.2
• /
• pp.250-254
• /
• 1998

Harmful micro algae isolated from Korean coastal waters, were tested with FITC-conjugated lectins and observed by epifluorescent microscopy to distinguish each other. Strain-specific sugar composition at the cell surface was suggested by the affinity of lectins to different microalgae. The micro algae Cochlodinium polykrikoides (CP-1) and Gymnodinium $A_3\;(GA_{3-1}\;1)$, are morphologically similar, but exhibited different binding activity with the lectins ECA, HPA and WGA. In Peridiniales, the micro alga Alexandrium tamarense (AT) bound HPA and WGA, but Scrippsiella trochoidea (ST-1) did not bind those lectins. Three species of Prorocentrum also exhibited different binding specificity with HPA, PHA and SBA. A non­toxic Korean isolate of Heterosigma akashiwo (HA-2) bound ConA, PEA and UEA. These results suggest that lectins are useful in discriminating morphologically similar species, as well as different species or strains within the same genus.

• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2000.05a
• /
• pp.381-381
• /
• 2000

Lectin binding assay was conducted on 3 A. tamarense isolates (AT-A, AT-2 and AT-6). Fatty acid composition of all 3 isolates was analyzed, and total carotenoid content and $\beta$-carotene were also determined. AT-A and AT-2 treated with different lectins in this study showed the positive response, whereas potentially toxic AT-6 did not bind DBA lectin, regardless of different growth phase, but conjugated ConA, PNA, RCA, SBA, UEA and WGA. It is possible that DBA is a desirable method for rapid and easy discrimination of highly toxic A. tamarense. AT-A, AT-2 and AT-6 comprised saturated fatty acids (49.0-61.9%), monounsaturated fatty acids (8.0-20.5%) and polyunsaturated fatty acids (23.2-30.5%). In particular, 22:6 (n-3) polyunsaturated fatty acid in AT-6 had a high abundance, compared with AT-A and AT-2. However, carotenoid content and $\beta$-carotene were not contributed to discriminate each isolate. Due to variability in biochemical composition at different isolates, possibly DBA and 22:6 (n-3) polyunsaturate fatty acid provide a good information for discrimination of AT-6.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.23 no.2
• /
• pp.118-151
• /
• 1997

Allergic contact dermatitis is a common skin disease resulting from specific immumologic sensitization to topically applied various allergen. Purpose of this study was to investigate skin morphologic chganges by light microscopic, changes of protein band by SDS-PAGE in the skin treated on the surfactant. Furthermore, lectin histochemistry is used to know the chagnges of the terminal sugar of the glycoconjugate in the skin treated on the surfactant. The results were as follows : The 1 day of treated group was indicated the enlargement of capillary, the 3 days of treated group was showed that the cytoplasm was eosinophlic by the pyknotic of nucleus. The 6 days of treated group was observed hyperkeratinoid, increased of inflammation cells to epithelium. The 9 days of treated group was appeares that serum crust was sheded and formed a acanthosis. But dermis layer was cytoplasmic vaculation and enlargement of intercellular space. The 12 days treated group made up new epidermis layer of seven layer and of observed an decreased in quantity of inflammation cells. The mast cell of degranulated type was increased in treated surfactnat. It was secreating granules hold histamin, serotonin and heparin. Accordingly, protein band of electrophortic phase was observed a sudden changes since 3 days treated group. At observeation of the cell-surface glycoconjugates, LCA, PNA, SBA and WGA lectin positive cells but inducated lectin negative cells in Con-A. Furthermore, PNA and SBA showed stroger positive reaction as treated surfactant group.

• Journal of Life Science
• /
• v.28 no.9
• /
• pp.1081-1091
• /
• 2018

The purpose of this study was to investigate the structure and the glycoconjugate properties of the olfactory mucosa in the rat after inhalation of xylene. Sprague-Dawley male rats were inhalated 300 ppm xylene for 5 times with 5 hours exposure. The olfactory mucosa in the nasal cavity was taken from the animals on 5, 10, 15, 20, and 30 days after inhalation of xylene. The properties of the glycoconjugates in the olfactory mucosa were investigated using 10 biotinylated lectins: (PSA, UEA I, PHA-L, GSL I B4, GSL I, PNA, ECL, SBA, GSL II, and sWGA). In the experimental groups, degenerative changes of the olfactory epithelium were observed until 20 days after inhalation of xylene. In the control group, the olfactory cells in the olfactory epithelium reacted with PSA, UEA I, PNA, SBA, and sWGA, the supporting cells reacted with PSA, PHA-L, GSL I, PNA, ECL, SBA, GSL II, and sWGA, and the Bowman's glands reacted with all 10 lectins. In the experimental groups, the reactivity to PSA, PNA, and SBA in the olfactory cells were decreased, and the reactivity to PSA, PNA, SBA, and GSL II in the supporting cells were decreased. And in the Bowman's gland, the reactivity to PSA, UEA I, GSL I, and sWGA were decreased. Conclusively, the olfactory mucosa was shown a lot of changes in the structure through degenerative process and in the properties of the glycoconjugates after inhalation of xylene. These results suggest that the sugar residues of the glycoconjugates in the olfactory mucosa can be changed by xylene inhalation.

• Proceedings of the Korea Society of Poultry Science Conference
• /
• 2001.11a
• /
• pp.69-70
• /
• 2001

Lectins have great potential as to determine the alternation of the distribution of cell surface carbohydrates during cellular development and differentiation. Here, we investigated the presence and distribution of cell surface carbohydrates on chicken primordial germ cells (PGCs) during the migration and gonadal stages using a variety of lectins. A total of six FITC-labelled lectins from several specificity classes were used: ConA (glucose/mannose), WGA (N-acetylglucosamine), STA (N-acetylglucosamine), DBA (N-acetylgalactosamine/galactose), UEA-I (fucose) and PHA-E (oilgosaccharide). As a results, PGC-specific binding was observed in STA. PGCs of migration stage (2.5- and 5.5-day embyos) were STA-positive whereas PGCs of 10-day embryonic gonad were not. The results suggest that N-acetylglucosamine residuse are present specifically in migrating chicken PGCs and changes during development.

• Journal of Life Science
• /
• v.18 no.6
• /
• pp.782-788
• /
• 2008

The aim of this work is to show differences in the pattern of glycoconjugate composition in the intestines of four teleostean species (Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis). We compared four regions of all species studied. The specimens were processed and stained with nine kinds of biotinylated lectins (DBA, SBA, PNA, BSL- I , RCA- I , sWGA, UEA- I , LCA and Con A). Except for Sebastes schlegeli, no differences between regions were observed. The intestinal epithelium of Halichoeres poecilopterus possessed D-glucose/mannose residues in all regions. ${\beta}$-N-acetyl-D-galactosamine was distinctive along the intestines, although the pattern of diversity was different in Sebastes schlegeli, Bryzoichthys lysimus, and Takifugu pardalis. Additionally, the occurrence of Galactose-${\beta}$-1,3-N-acetyl-D-galactosamine and ${\alpha}$-D-galactose were confirmed in the proximal, middle, and distal intestine of Sebastes schlegeli, while rectal intestine lacked these sugar residues. Along with ${\beta}$-N-acetyl-D-galactosamine, ${\beta}$-N-acetyl-D-glucosamine and D-glucose/mannose were also determined in Bryzoichthys lysimus. Galactose-${\beta}$-1,3-N-acetyl-D-galactosamine, D-galactose, and D-glucose/mannose were also present in Takifugu pardalis.

• Environmental Mutagens and Carcinogens
• /
• v.18 no.2
• /
• pp.116-122
• /
• 1998

The present investigation has been performed to elucidate the effect of pretreatment with low dose of ultraviolet radiation (UV), ethyl methansulfonate (EMS), and bleomycin (BLM) on cell survival and lectin-binding glycoconjugates of plasma membrane in HeLa cells treated with mutagen. The percentage of survival of cells pretreated with 1 mM EMS following treatment with 10 mM EMS was higher than that of cells treated with 10 mM EMS alone. Wheat germ agglutinin (WGA) staining intensity of cells pretreated with 1 mM EMS and subsequently treated with 10 mM EMS was stronger than that of cells treated with 10 mM EMS alone. But, succinylated wheat germ agglutinin (sWGA) staining intensity of cells pretreated with 1 mM EMS and subsequently treated with 10 mM EMS was similar to that of cells treated with 10 mM EMS alone. These results suggest that the acquired resistance to EMS is related to the glycoconjugates containing sialic acid of plasma membrane involved in multidrug resistance or adaptive response in HeLa cells.