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http://dx.doi.org/10.5352/JLS.2008.18.6.782

Characterization and Distribution of Glycoconjugates in the Intestines of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis.  

Jeong, Gil-Nam (Biology Education Major, Division of Science Education, College of Education, Pusan National University)
Publication Information
Journal of Life Science / v.18, no.6, 2008 , pp. 782-788 More about this Journal
Abstract
The aim of this work is to show differences in the pattern of glycoconjugate composition in the intestines of four teleostean species (Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis). We compared four regions of all species studied. The specimens were processed and stained with nine kinds of biotinylated lectins (DBA, SBA, PNA, BSL- I , RCA- I , sWGA, UEA- I , LCA and Con A). Except for Sebastes schlegeli, no differences between regions were observed. The intestinal epithelium of Halichoeres poecilopterus possessed D-glucose/mannose residues in all regions. ${\beta}$-N-acetyl-D-galactosamine was distinctive along the intestines, although the pattern of diversity was different in Sebastes schlegeli, Bryzoichthys lysimus, and Takifugu pardalis. Additionally, the occurrence of Galactose-${\beta}$-1,3-N-acetyl-D-galactosamine and ${\alpha}$-D-galactose were confirmed in the proximal, middle, and distal intestine of Sebastes schlegeli, while rectal intestine lacked these sugar residues. Along with ${\beta}$-N-acetyl-D-galactosamine, ${\beta}$-N-acetyl-D-glucosamine and D-glucose/mannose were also determined in Bryzoichthys lysimus. Galactose-${\beta}$-1,3-N-acetyl-D-galactosamine, D-galactose, and D-glucose/mannose were also present in Takifugu pardalis.
Keywords
Intestine; glycoconjugate; lectin; teleost; goblet cell;
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1 Fiertak, A. and W. M. Kilarski 2002. Glycoconjugates of the intestinal goblet cells of four cyprinids, Cell Mol. Life Sci. 59, 1724-1733   DOI   ScienceOn
2 Çinar, K. and N. Şenol. 2006. Histological and histochemical characterization of the mucosa of the digestive tract in flower fish (Pseudophoxinus antalyae). Anat. Histol. Embryol. 35, 147-151   DOI   ScienceOn
3 Etzler, M. E. and M. L. Branstrator. 1974. Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectin. J. Cell Biol. 62, 329-343   DOI   ScienceOn
4 Goldstein, I. J. and C. E. Hayes. 1978. The lectins: carbohydrate- binding proteins of plant and animal. Adv. Carbohydr. Cytochem. Biochem. 35, 127-340   DOI   ScienceOn
5 Jeong, G. N., G. J. Jo, E. H. Lee and U. B. Jo. 2005. Histochemical study of the intestinal mucosubstances in Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus and Takifugu pardalis. Kor. J. Life Sci. 15, 707-714   과학기술학회마을   DOI   ScienceOn
6 Jeong, G. N and U. B. Jo. 2007. Histochemical studies of the intestinal mucosubstances in Agramus agramus, Inimicus japonicus, Epinephelus chlorostigma, and Helicolenus dactylopterus. Kor. J. Life Sci. 17, 1669-1674   과학기술학회마을   DOI   ScienceOn
7 Watanabe, M., T. Muramatsu, H. Shirane and K. Ugai. 1981. Discrete distribution of binding sites for Dolichos biflorus agglutinin (DBA) and for peanut agglutinin (PNA) in mouse organ tissue. J. Histochem. Cytochem. 29, 779-790   DOI   ScienceOn
8 Andrew, W. and C. P. Hickman. 1974. Histology of the Vertebrates. pp. 70, Mosby Co., Saint Louis
9 Schulte, B. A. and S. S. Spicer. 1983. Light microscopic histochemical detection of terminal galactose and N-acetylgalactosamine residues in rodent complex carbohydrates using a galactose oxidase-Schiff sequence and peanut lectin- horseradish peroxidase conjugates. J. Histochem. Cytochem. 31, 19-24   DOI   ScienceOn
10 Ito, S. 1969. Structure and function of the glycocalyx. Fed. Proc. 28, 12-25
11 Plenderleith, M. B., L. L. Wright and D. J. Snow. 1992. Expression of lectin binding in the superficial dorsal horn of the rat spinal-cord during prenatal and postnatal- development. Dev. Brain Oes. 68, 103-109   DOI   ScienceOn
12 Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J. D. Watson. 1989. Molecular Biology of the Cell. pp. 298, 2nd eds., Garland Publishing, New York
13 Jo, U. B. and H. C. Park. 1972. A comparative histochemical study on the mucosa of the digestive tract of the amphibians and fishes. J. Pusan National Univ. 13, 383-403
14 Prasad, T. N., D. K. Sahu and A. Moitra. 1992. Histochemical observations on the nature of mucous secreting cells in the gastrointestinal tract of Channa punctatus. J. Freshwat. 4, 267-272
15 Bohinski, R. C. 1983. Modern Concepts in Biochemistry. pp. 198, 4th eds., Allyn and Bacon, Boston
16 Gupta, B. J. 1989. The relationship of mucoid substances and ion water transport, with new data on intestinal goblet cells and a model for gastric secretion. Symp. Soc. Exp. Biol. 43, 81-110
17 Reifel, C. W. and A. A. Travill. 1979. Structure and carbohydrate histochemistry of the intestine in ten teleostean species. J. Morphol. 162, 343-360   DOI
18 Veggetti, A., A. Rowlerson, G. Radelli, S. Arrighi and C. Domeneghini. 1999. Post-hatching development of the gut and lateral muscle in the sole. J. Fish Biol. 55, 44-65   DOI
19 Hsu, S. M. and L. Raine. 1982. Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections. J. Histochem. Cytochem. 30, 157   DOI   ScienceOn
20 Krementz, A. B. and G. B. Chapman. 1975. Ultrastructure of the posterior half of the intestine of the Channel Catfish, Ictalurus punctatus. J. Morphol. 145, 441-482   DOI
21 Smith, L. S. 1976. Digestion in teleost fishes. Bull. Jap. Soc. Sci. Fish 45, 409-449
22 Byeon, K. A. and U. B. Jo. 1985. Histochemical properties on mucosubstances of the intestine in Sparus swinhonis (Günther), Erosa erosa (Langsdorf) and Sebasbes inermis (Cuvier et Valenciennes). J. Sci., Pusan National Univ. 40, 251-269
23 Kim, K. L., S. C. Moon, S. Y. Baek, J. B. Kim and B. S. Kim. 1995. Differential glycoconjugate expression in the goblet cells of the rat proximal colon. J. Pusan Med. Coll. 35, 1-7
24 Sharon, N. and H. Lis. 1989. Lectins as cell recognition. Science 177, 949-959   DOI
25 Anderson, T. A. 1986. Histological and cytological structure of the gastrointestinal tract of the luderick, Girella tricuspidata in relation to diet. J. Morphol. 190, 109-119   DOI
26 Madrid, J. F., M. T. Castells, J. A. Marin and L. M. Pastor. 1989. Characterization of glycoconjugates in the intestinal mucosa of vertebrates by means of lectin histochemistry. Acta. Histochem. Cytochem. 22, 1-14   DOI
27 Kirschner, L. B. 1978. External charged layer and Na$^+$ regulation, pp. 310-321, In Alfred Benzon Symposium 11, Osmotic and Volume Regulation, Academic Press, New York
28 Gutiérrez, M. and M. C. Sarasquete. 1986. Distribución histoquímica de carbohidratos y proteínas en estómago e intestino de Anguilla anguilla L., 1758 (Osteichthyes, Anguillidae) de las salinas de Cádiz. Iav. Preq. 50, 553-564
29 Loretz, C. A. 1995. Electrophysiology of ion transport in teleost intestinal cells, pp. 25-56, In Wood, C. H. and T. J. Shuttelworth (eds.), Cellular and Molecular Approaches to Fish Ionic Regulation, Academic Press, London
30 Patt, D. I. and G. R. Patt. 1969. Comparative Vertebrate Histology. pp. 146-205, Harper and Row, New York
31 Chakrabarti, P., S. Ganguly and D. K. Mandal. 1994. Histochemical studies of the digestive system of freshwater cichlid fish Oreochormis mossambicus (Peters). J. Freshwat. 6, 63-69
32 Zhou, X. H., H. Kawakami and H. Hirano. 1992. Changes in lectin binding pattern of Leydig-cells during fetal and postnatal development in mice. J. Histochem. 24, 354-360   DOI   ScienceOn
33 Harris, J. and S. Hunt. 1973. Epithelial mucins of the atlantic salmon (Salmon salar L.). Biochem. Soc. Trans. 1, 153-155   DOI
34 Jo, U. B. 1974. Histochemical studies on the acid mucosubstances in the mucosa of alimentary tract of lower vertebrates. Kor. J. Anat. 7, 19-30