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Screening for Effective Organic Farming Materials for the Control of Cucumber Scab Caused by Cladosporium cucumerinum (오이 검은별무늬병 방제에 효과적인 유기농업자재 선발)

  • Park, So-Hyang;Hong, Sung-Jun;Shim, Chang-Ki;Kim, Min-Jeong;Park, Jong-Ho;Han, Eun-Jung;Kim, Yong-Ki
    • Research in Plant Disease
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    • v.23 no.2
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    • pp.159-167
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    • 2017
  • This study was conducted to evaluate the control efficacy of the organic farming materials on cucumber scab caused by Cladosporium cucumerinum PT1 (KACC 48094). The antifungal activities in vitro as well as the suppressive effect of 43 organic farming materials on the spore germination and germ tube growth by inoculating spore suspension on cucumber seedlings in vivo were investigated. Thirteen organic farming materials inhibited the mycelial growth of C. cucumerinum and nine of these were microbial agents. In the screening using cucumber seedlings, six organic farming materials were very effective with control efficacy value of 90%. Among them, Bacillus amyloliquefaciens M27 provided suppressive effect on both mycelial growth and spore germination against cucumber scab. Finally, nine organic farming materials were selected to test the protective and curative effects, and all chosen organic farming materials significantly suppressed disease incidence when applied in the preventive action, in comparison with the curative action. Especially, Bordeaux mixture I and III gave excellent protective control efficacy with control values of 96.7% and 73.3%, respectively, whereas its curative control effect was significant low. Among these, only Thymus quinquecostatus+Sophora extract showed curative activity, although the control value was as low as 50%. This study suggests that cucumber scab can be controlled by some organic farming materials in the farmhouses under comparatively cold and wet condition and protective treatment is more important and efficient.

Establishment of Herbicide Screening Methods for Reed (Phragmites communis Trin.) Control - I. Propagation of Reed (갈대(Reed, Phragmites communis Trin.)의 방제를 위한 제초제 스크리닝방법의 확립 - I. 갈대의 육묘)

  • Hwang, I.T.;Choi, J.S.;Lee, H.J.;Hong, K.S.;Cho, K.Y.
    • Korean Journal of Weed Science
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    • v.16 no.1
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    • pp.21-27
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    • 1996
  • This experiment was conducted to find out an effective propagation method for reed(Phragmites communis Trin.), ensuring a continuous herbicide screening for reed control. Reed propagation methods were compared under a greenhouse condition using tour different materials; seeds, rhizomes, depressed stolons of P. japonica Steud., and stem cuttings. Although reed seeds were easy to harvest and store, their germination rate(${\leq}$5%) was very low and seedling growth from the seeds was slow. Rhizomes were difficult to harvest and their harvest time was limited from November to March. Furthermore, reed propagation using rhizomes had problems of a relatively low germination rate(46%), no uniformity in size and shape, individual differences at the early stage of growth, and difficulties in material storage. Rate of reed growth from rhizomes was higher in commercial soil mix(Boo Nong soil) than in sand or in sand+upland soil(1:1). Depressed stolons of P. japonica had a moderate germination rate(65%) and were relatively easy to harvest. However, their harvest time was limited only from August to September. Propagation method using stem cuttings had several advantages over the above methods using other materials. Reed plants could uniformly be propagated from the stem cuttings with a relatively high germination rate(75%). Stem cuttings of central nodes showed a higher germination rate compared to those of upper or lower nodes. Stem cuttings from the field should be used immediately after harvest, since their germination rate decreased rapidly when they were stored under a wet- or a dry-refrigerated condition. Furthermore, the germination of stem cuttings tended to decrease when they were collected from the field after August. This indicates that there is a limitation of harvest time for stem cuttings. However, a year-round propagation of reed using stem cuttings is possible if parent plants are grown in a greenhouse, and thus herbicide screening for reed control could continuously be performed.

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Zooplankton Abundance in Korean Waters (한국근해 동물성 부유생물의 주요군의 양적 분포)

  • Park, Joo-suck
    • 한국해양학회지
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    • v.8 no.1
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    • pp.33-45
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    • 1973
  • Plankton samples used for the present study were collected by the NORPAC net during the CSK cruises in the Korean waters in March and August, 1967. Regional and seasonal variations in the zooplankton biomass (wet weight, mg/㎥) were noticed in the Korean waters. In March the highest biomass, 130mg/㎥ on the average, occurred in the southern part of Japan Sea, but the lowest biomass of less than 50mg/㎥ occurred in the Yellow Sea and the western sea of Cheju Island Contrally, in August, the average biomass of 120mg/㎥ was measured in the Yellow Sea, the western sea of Cheju Island and the coastal waters of southern Korea, while the biomass of Japan Sea was the lowest of the regions surveyed. In comparison with the zooplankton biomass, total number of zooplankton per cubic meter of water strained also showed regional and seasonal fluctuations. In general, variations in the number of zooplankton specimens follows the same trend as in the biomass. The largest number, up to 800mg/㎥ on the average, occurred in the southern part of Japan Sea in March and the lowest number, less than 200mg/㎥ occurred in the Yellow Sea and the western sea of Cheju Island. In August, as shown by the biomass fluctuations, the largest number of zooplankton 850mg/㎥ on the average occurred in the Yellow Sea, the western sea of Cheju Island and the coastal region of southern Korea. But the lowest number of less than 500mg/㎥ was found in the Japan Sea. Among the various groups of zooplankton examined, the following were dominant components of the zooplankton population: Copepoda, Chaetognatha, Siphonophora, Euphausiacea, Cladocera, Appendicularia, and Amphipoda. The zooplankton conposition was significantly differed between the Japan Sea and Yellow Sea. Copepods which usually occupied over 66% in the Japan Sea and thd Korean Strait samples occupied only 42% of the catches in August, while cladocerans and chaetognaths were relatively abundant, i. e., 15 and 18% of the total organisms. The most dominant species of copepods and chaetognaths were Paracalanus parvus, Oithona similis, Acartia clausi, Calanus helgolandicus, Sagitta enflata, S. bedoti, S. elegans and S. crassa.

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Studies on the Effect of Water Content, Curing Temperature and Grain Size Distribution of Soils on Unconfined Compressive Strength of Soil-Cement Mixtures. (함수비, 양생온도 및 흙의 입도가 Soil-Cement의 압축강도에 미치는 영향에 관한 연구(I))

  • 김재영;강신업
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.19 no.1
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    • pp.4312-4322
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    • 1977
  • In order to investigate the effect of the water content and the accelerated curing on the strength of the soil-cement mixtures, laboratory test of soil cement mixtures was performed at five levels of water content, four levels of accelerated curing temperatures, three levels of normal curing periods, and six levels of accelerated curing time. Also this study was carried out to investigate the effect of grain size distribution of 21 types of soils on the strength of soil-cement mixtures at four levels of cement content and three levels of curing time. The results are summarized as follows: 1. Optimum moisture content increased with increase of the cement content, but maximum dry density was changed ununiformly with cement content. Water content corresponding to the maximum strength was a little higher than the optimum moisture content along the increase of cement content. 2. In molding the specimens with the optimum moisture content, the maximum strength appeared at the wet side of the optimum moisture content. 3. According to increase of curing temperature as 30, 40, 50, and 60$^{\circ}C$, unconiiend compressive strength of soil-cement mixtures increased, the rate of increase at the early curing period was large, and approximately 120 hours was suifficient to harden soil-cement mixtures completely. 4. The strength of soil-cement mixtures at the curing temperature of 10$^{\circ}C$ decreased at the rate of 30 to 50 percent than at the curing temperature of 20$^{\circ}C$, and the strength of soil-cement mixtures at the curing temperature of 0$^{\circ}C$ increased a little with increase of curing time. 5. Although the strength of soil-cement mixtures seemed to be a little affected by the temperature difference between day time and night, it was recommended that reasonable working period was the duration from July to August of which average maximum temperature of Korea was approximately 30$^{\circ}C$. 6. Accelerated curing time corresponding to the normal curing time of 28-day was shorten with increase of curing temperature, also it was a little affected by the cement. Accelerated curing time that the strength of soil-cement mixtures for the cement of 9 percent and the curing temperature of 60was shorten with increase of curing temperature, also it was a little affected by the cement. Accelerated curing time that the strength of soil-cement mix- tures for the cement of 9 percent and the curing temperature of 60$^{\circ}C$ was 45 hours at the KY sample, 50 hours at the MH, 40 hours at the SS, and 34 hours at the JJ respectively. 7. Accelerated curing time was depended upon the grain size distribution of soil, it decreased with increase the percent passing of No. 200 sieve. 8. Relationship between the normal curing times and the accelerated curing times showed that there was a linear relationship between them, its slope decreased with increase of curing temperature. 9. The most reasonable soil of the soil-cement mixtures was the sandy loam which was a well graded soil. Assuming the base of road requiring 7-day strength of 21 kg/$\textrm{cm}^2$ being used, the soil-cement mixtures could be obtained with adding 6 percent of cement in such a sails S-7, S-8, S-9, S-10, S-11, S-12, S-13. 10. The regression equation between the 28-day and the 7-day strength was obtained as follow; q28=1.12q7,+6.5(r=0.96).

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Air Pollutants Levels and Physiological Variation of Ginkgo biloba in Chuncheon (춘천지역의 대기오염도와 은행나무의 생리적 변화에 관한 연구)

  • Lee Sang-Deok;Joo Yeong-Teuk;Han Jin-Seok
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.7 no.2
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    • pp.141-147
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    • 2005
  • This study investigated air pollutant levels and physiological variation of Ginkgo biloba in Chuncheon. The results were as follows: The annual average concentrations of $SO_2,\;NO_2\;and\;PM10$ were 0.004ppm, 0.013 ppm and $66{\mu}g/m^3$, respectively. The volume weighted average concentrations of ionic components were $SO_4\;^{2-}\;3.584 mg/m^3,\;NO_3^-\; 2.803 mg/m^3,\;Cl^-\;1.485 mg/m^3\;and\;NH_4\;^+\;0.998 mgg/m^3$ in precipitation. The annual wet deposition amount of the major ions was shown to be $SO_4^{2-}\;3.865g/m^2/yr,\;NO_3^-\;2.924g/m^2/yr,\;Cl^-\;2.773g/m^2/yr\;and\; NH_4\;^+\;1.485 g/m^2/yr$ during this study period. The seasonal averaged pH in leaves were spring pH 5.9 0.5, summer pH 5.5 0.4 and fall pH 5.1 0.3. The seasonal average water soluble sulfur content in leaves were spring 0.012 0.004%, summer $0.012\;0.002\%\;and\;fall\;0.020\;0.007\%$. The seasonal average water soluble sulfur content in bark were spring $0.0071\;0.0003\%,\;summer\; 0.0066\;0.0004\%,\;fall\;0.0063\;0.0004\%\;and\;winter\;0.0071\;0.0003\%$.

Responses of Root Growth Characters to Waterlogging in Soybean [Glycine max (L.) Merrill] (과습에 따른 콩 지하부 생육반응)

  • Lee, Jae-Eun;Kim, Hong-Sig;Kwon, Young-Up;Jung, Gun-Ho;Lee, Chun-Ki;Yun, Hong-Tai;Kim, Chung-Kon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.1
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    • pp.1-7
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    • 2010
  • Stress from excess water is one of the most harmful limiting factor in soybean yield during the wet season under the climate conditions. Soybean is very sensitive to excess water compare to other crops. This experiment was carried out to identify the growth responses for establishing a screening system related to waterlogging tolerance in soybean from 2003 to 2004. The root dry weight accumulation rate of per day for 21 days after waterlogging at V5 stage was the highest in Pungsannamulkong (47~56% of control) and was the lowest in Jangyeobkong (26~27% of control). The nodules dry weight recovery rate was the highest in Pungsannamulkong (83~91% of control), while the lowest in Myungjunamulkong (48~66% of control). After waterlogging, recovery rate of roots was high, which increased the root/shoot ratio of Pungsannamulkong, which also produced significantly more adventitious roots than in Jangyeobkong. The percentage of adventitious roots fresh weight to the total roots fresh weight was the lowest in Myungjunamulkong (14%), while the highest in Pungsannamulkong (38%). This results implies that the water and nutrients absorbing ability of Pungsannamulkong is more higher than that of Myungjunamulkong during late growth period.

Effect of Vinclozolin on the Onset of Puberty in Immature Female Rats (미성숙 암컷 흰쥐의 사춘기 개시에 미치는 Vinclozolin의 영향)

  • An, Na-Kyung;Lee, Sung-Ho
    • Development and Reproduction
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    • v.11 no.3
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    • pp.245-251
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    • 2007
  • Vinclozolin(VCZ), a systemic dicarboximide fungicide, has been used in the control of diseases caused by microorganism of some species in fruits, vegatables and ornamental plants. Although VCZ itself is a very weak antagonist for androgen receptor binding, both melabolites M1 and M2 are effective antagonists. The present study was undertaken to examine whether prepubertal exposure to VCZ affects on the onset of puberty and the associated reproductive parameters such as hormone receptor expressions in female rats. VCZ(10 mg/kg/day) was administered daily from postnatal day 21(PND 21) through the day when the first vaginal opening(V.O.) was observed. Gross anatomy and weight of reproductive tissues were compared to test the VCZ's effects on the cell proliferation. Furthermore, histological studies were performed to assess the structural alterations in the tissues. To determine the transcriptional changes in progesterone receptor(PR), total RNAs were extracted and applied to the semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). As a result, delayed V.O. was shown in the VCZ group(PND $34.00{\pm}1.22$) compared to the control group(PND $38.20{\pm}1.92$; p<0.01). VCZ treatment significantly decreased the wet weight of ovaries and uteri compared to the control group(p<0.01). Graafian follicles and corpora lutea were observed only in the ovaries from the control animals, while numerous primary, secondary follicles and small atretic follicles were observed in the ovaries from VCZ group. Similarly, hypotrophy of luminal and glandular uterine epithelium was found in the VCZ group. In the semi-quantitative RT-PCR studies, the transcriptional activity of PR in ovary(p<0.01) from VCZ group were significantly lower than those from the control group while in uterus were similar compared with the control group. The present studies demonstrated that the acute exposure to VCZ during the critical period of prepubertal stage could inactivate the reproductive system resulting delayed puberty in female rats.

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Origin, Age and Sedimentation Rate of Mid-Geum River Sediments (금강 중류 하상 퇴적층의 기원과 형성시기 및 퇴적율)

  • Oh, Keun-Chang;Kim, Ju-Yong;Yang, Dong-Yoon;Hong, Sei-Sun;Lee, Jin-Young;Lim, Jae-Soo
    • Economic and Environmental Geology
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    • v.43 no.4
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    • pp.333-341
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    • 2010
  • Fluvial sediments are widely distributed in present and old river-beds of the mid-Keum River, the tributaries of which are the Yugu and Jeongan Rivers. The basement of the mid-Keum River area consists of Mesozoic granites which are easily eroded compared to Precambrian gneisses, which are exposed in the upper-Keum River area. The provenance of the fluvial sediments includes both the Precambrian gneisses and Mesozoic granites, which occur in the catchment of the mid-Keum River. The coarse-grained sediments were probably transported from the river-beds and the overbank floodings of the main Keum River and its tributaries when the climate was warm and wet. The oldest mud deposits were dated at ca. 9,400 yr BP by the radiocarbon method. It has been estimated that the sand deposits below the dated muds were formed in a period from the Late Pleistocene to the Early Holocene. However we have revealed that the major part of the present river-bed sediments was formed at ca. 3,000-6,000 yr BP, i.e., in the mid- to late Holocene, when summer monsoon was very strong due to climatic changes. We have calculated fluvial sedimentation rates of 0.12-0.16 cm/yr and 0.02-0.09 cm/yr for borehole KJ-29 river-bed sediments and borehole KJ-28 floodplain deposits, respectively. We conclude that the sedimentation rate is higher near the present stream channel than near the floodplain.

Mineral Identification and Field Application by Short Wave Infrared (SWIR) Spectroscopy (단파장적외선 분광분석법을 이용한 광물동정과 현장적용성)

  • Kim, Chang Seong;Kim, Yong-Hwi;Choi, Seon-Gyu;Ko, Kwang-Beom;Han, Kyeong-Soo
    • Economic and Environmental Geology
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    • v.50 no.1
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    • pp.1-14
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    • 2017
  • The analytical conditions including surface state, moisture effect, and device condition were investigated for applying Short Wave Infrared(SWIR) spectroscopy to the field survey. Among the three surface state of samples (exposed surface, cutting face and powder), both spectra from the exposed surface and cutting face are almost identical whereas spectral variation was detected in powder sample. Over 24-hours-dryring of the wet sample at room temperature, the samples show a similar spectrum with that of dry condition. The result suggests that outcrop samples mighty be dried for 24 ~ 48 hours depending on the wetness of outcrop. The bright minerals could produce stable spectra with 10 times measurements as default value of the device under SWIR spectroscopy but the dark minerals would require about 10 seconds, which corresponds to 100 times measurements to get the reliable spectra. The position and shape 2,160 ~ 2,330 nm and/or other spectral features of hydrothermal alteration minerals by SWIR spectroscopy could be used for a classification of hydrothermal alteration zone in the field. Absorption peaks in 2,160 ~ 2180 nm are useful for identifying (advanced) argillic zone by spectral characteristics of kaoline, dickite, pyrophyllite, and alunite. Absorption peaks in 2,180 ~ 2,230 nm are able to define muscovite, sericite, and smectite, which are key alteration minerals in phyllic zone. Absorption peaks in 2,230 ~ 2,270 nm can be used to recognize prophylitic zone where chlorite and epidote occur. Absorption peaks of other principle minerals such as talc, serpentine, amphibole, and carbonate group are mainly detected within the wave length of 2,270 ~ 2,330 nm. This result indicates that the spectra of these minerals need to be carefully interpreted.

Composting of Compostable Wet Household Wastes Using Waste Newspapers as Humidity Amendment (수분개선제로 폐신문지를 이용한 가정쓰레기의 퇴비화)

  • Yun, Eun-Jin;Park, Ju-Won;Seo, Jeoung-Yoon
    • Korean Journal of Environmental Agriculture
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    • v.19 no.1
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    • pp.32-37
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    • 2000
  • Because the household garbage had high water contents, it is difficult to compost it without an additive. Therefore, this study was performed to investigate possibility of using the waste newspapers as a humidity conditioner for the household garbage composting. The maximum temperature was $66.0^{\circ}C$ in spring, $69.2^{\circ}C$ in summer, $60.9^{\circ}C$ in fall and $56.0^{\circ}C$ in winter for composting periods. The seasonal pH value reached around 8.5 after 1 week and then repeated fluctuation at the narrow range in spring and fall, while it was stabilized at the range of $8{\sim}9$ after increasing to 8.5 after 1 week in winter. The water content was reduced little in winter, while decreased significantly in the other seasons. The water content after 8 weeks was 22.2% in spring, 47.6% in summer, 25.5% in fall and 72.5% in winter. The mass was reduced rapidly during the first week of each season, but it did not show much decrease. The volume reduced after 8 weeks to 59%, 32%, 27%, and 34% in spring, summer, fall and winter respectively. Organic matter content decreased over the four seasons. Nitrogen contents were in the range of 0.7% to 2.2% during the four seasons. The contents of inorganic compounds based on dry matyter were in the range of $0.94{\sim}2.59%\;P_2O_5$, $1.23{\sim}1.87%\;CaO$, $0.37{\sim}0.46%\;MgO$, $0.55{\sim}1.98%\;K_2O$. Concentration of heavy metals(Hg, Cd, Pb, Cu, Cr, Zn, As) based on dry matter were less than the limiting value of the by-product compost.

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