• Title/Summary/Keyword: WAI

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The Existence of a Putative Regulatory Element in 3'-Untranslated Region of Proto-oncogene HOX11's mRNA

  • Li, Yue;Jiang, Zhao-Zhao;Chen, Hai-Xu;Leung, Wai-Keung;Sung, Joseph J.Y.;Ma, Wei-Jun
    • BMB Reports
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    • v.38 no.4
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    • pp.500-506
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    • 2005
  • HOX11 encodes a homeodomain-containing transcription factor which directs the development of the spleen during embryogenesis. While HOX11 expression is normally silenced through an unknown mechanism in all tissues by adulthood, the deregulation of HOX11 expression is associated with leukemia, such as T-cell acute lymphoblastic leukemia. The elucidation of regulatory elements contributing to the molecular mechanism underlying the regulation of HOX11 gene expression is of great importance. Previous reports of HOX11 regulatory elements mainly focused on the 5'-flanking region of HOX11 on the chromosome related to transcriptional control. To expand the search of putative cis-elements involved in HOX11 regulation at the post-transcriptional level, we analyzed HOX11 mRNA 3'-untranslated region (3'UTR) and found an AU-rich region. To characterize this AU-rich region, in vitro analysis of HOX11 mRNA 3'UTR was performed with human RNA-binding protein HuR, which interacts with AU-rich element (ARE) existing in the 3'UTR of many growth factors' and cytokines' mRNAs. Our results showed that the HOX11 mRNA 3'UTR can specifically bind with human HuR protein in vitro. This specific binding could be competed effectively by typical ARE containing RNA. After the deletion of the AU-rich region present in the HOX11 mRNA 3'UTR, the interaction of HOX11 mRNA 3'UTR with HuR protein was abolished. These findings suggest that HOX11 mRNA 3'UTR contains cis-acting element which shares similarity in the action pattern with RE-HuR interactions and may involve in the post-transcriptional regulation of the HOX11 gene.

Triple Negative Status is a Poor Prognostic Indicator in Chinese Women with Breast Cancer: a Ten Year Review

  • Ma, K.K.;Chau, Wai Wang;Wong, Connie H.N.;Wong, Kerry;Fung, Nicholas;Lee, J.T. Andrea;Choi, L.Y. Catherine;Suen, Dacita T.K.;Kwong, Ava
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.5
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    • pp.2109-2114
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    • 2012
  • Background: Ethnic variation in tumor characteristics and clinical presentation of breast cancer is increasingly being emphasized. We studied the tumor characteristics and factors which may influence the presentation and prognosis of triple negative breast cancers (TNC) in a cohort of Chinese women. Methods: A prospective cohort of 1800 Chinese women with breast cancer was recruited in a tertiary referral unit in Hong Kong between 1995 and 2006 and was followed up with a median duration of 7.2 years. Of the total, 216 (12.0%) had TNC and 1584 (88.0%) had non-TNC. Their clinicopathological variables, epidemiological variables and clinical outcomes were evaluated. Results: Patients with TNC had similar age of presentation as those with non-TNC, while presenting at earlier stages (82.4% were stage 1-2, compared to 78.4% in non-TNC, p=0.035). They were likely to be associated with grade 3 cancer (Hazard Ratio(HR)=5.8, p<0.001). TNC showed higher chance of visceral relapse (HR=2.69, p<0.001), liver metastasis (HR=1.7, p=0.003) and brain metastasis (HR=1.8, p=0.003). Compared with non-TNC group, TNC had similar 10-year disease-free survival (82% vs 84%, p=0.148), overall survival (78% vs 79%, p=0.238) and breast cancer-specific mortality (18% vs 16%, p=0.095). However, TNC showed poorer 10-year stage 3 and 4 specific survival (stage 3: 53% vs. 67%, p=0.010; stage 4: 0% vs. 40%, p=0.035). Conclusions: Chinese women with triple negative breast cancer do not have less aggressive biological behavior compared to the West and presentation at a later stage results in worse prognosis compared with those with non triple negative breast cancer.

An Analysis of Panel Data on the Web-accessibility Policies of Local Governments in Korea (우리나라 웹 접근성 정책 영향요인 분석 - 16개 광역자치단체 패널데이터를 중심으로 -)

  • JIN, Sangki;HYUN, Joonho
    • Informatization Policy
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    • v.18 no.4
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    • pp.42-58
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    • 2011
  • This paper starts from one question: what are the key factors of the web accessibility policy, which is significant for realizing equity in the web and enhancing human dignity in the information society. To find significant factors for complying with web accessibility, this paper analyzes panel data of 16 Korean local governments (for five years: 2004-2009) according to the research design which is based on the demand and supply balance model and consists of four variables : 'legal and institutional environment (including legal infrastructure)', 'financial foundation (fiscal self-reliance ratio)', 'policy inputs (amount of imformatization budget, employee of information experts)'and 'policy demand (internet usage rate, the number of disabled people and elderly people)'. From the results of this study, this paper can explain the mechanism and impact factors on the web accessibility policy of Korean local governments. Some factors are critical to improve web accessibility: (1) the importance of policy demand, (2) the importance of policy inputs, (3) the importance of legal and institutional environment. Finally, this paper concludes with some suggestions to enhance the web accessibility capacity for Korean local governments: (1) improve awareness on web accessibility, (2) develop a standard and invest R&D on web accessibility, (3) foster experts in web accessibility.

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New Directions in Communicating Better Nutrition to Older Adults

  • Guldan, Georgia-Sue;Wendy Wai-Hing Hui
    • Journal of Community Nutrition
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    • v.2 no.1
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    • pp.62-70
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    • 2000
  • Nutrition education should be an important component of ongoing health promotion for older adults and their caregivers. This is because prevention through sound nutrition and food hygiene practices and regular excercise is the most cost-effective way to reduce risks for and deal with their major health problems. nutritions education services should effectively promote optimum intake and successful self-care. Unfortunately, however relative to other vulnerable groups, nutrition education for older adults has not been systematically developed or evaluated. Usually oder adults care a lot about their health, so this should be a relatively easy group to teach - but their increasing numbers, longevity and great diversity with respect to health, physical, and economic status and educational level present challenges. Some older adults may not perceive they would benefit from nutrition education, so interesting and motivating them is a challenge. The food and nutrition knowledge of older people has been acquired through a lifetime of experience. For most older adults in the Asian region, their sources are restricted by their restricted education, so that their major sources of information have been informal sources, such as television, radio, friends, family, and perhaps newspapers and magazines if they are literature. Nonetheless, dietary advice for older people should build on their existing knowledge and ingrained values. It should provide information useful in daily food selection, and focus on food, not nutrients - the same foods and groups considered appropriate for younger people, with consistent messages as given throughout the population. Attention must also be paid to discovering learning styles in older people. When we teach in schools, the young students are a captive audience resigned to their learning role. Learning by an older adult, however, reflects an effort to meet his or her perceived needs. Therefore, nutrition education should be a positive experience in a non-threatening environment, relaxed and non-competitive, and perhaps even social environment. The messages also need to be practical and achievable. A needs assessment is essential, because our ability to provide the most effective nutrition education will depend on our success in matching the needs, both perceived and unperceived. of this vulnerable group. Therefore, go to the potential older learners to assess their interest and preferences. Nutrition education activities for older adults are widespread, but few have been evaluated. Evaluation is therefore also recommended, particularly when new methods are used. Tips from other countries for giving successful nutrition education will be given, including some examples of applications as attempted in Hong Kong. Research needs will also be described. In conclusion, successful nutrition education for older adults depends on positive needs-based messages. This is may be hard to do, as few good examples are available to illustrate these principles.

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Chlorella cultivation for mass culture of rotifer, Brachionus Plicatilis I. Selection of suitable Chlorella species (Rotifer (Brachionus Plicatilis) 사육을 위한 Chlorella의 배양 1. 적종 Chlorella의 선택)

  • HUR Sung Bum;KIM Hyun-Jun
    • Journal of Aquaculture
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    • v.1 no.2
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    • pp.135-143
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    • 1988
  • Rotifers became an important live food for fish larvae, especially for marine fishes, and Chlorella has been used as a very useful food for the mass culture of rotifer. However, not many tests for suitable Chorella species for the mass culture of rotifer were done and many of Chlorella sp. have been used without consideration of species for this purpose. There-fore, two species of marine Chlorella and four species of freshwater Chlorella were tested to select suitable species for the mass culture of a rotifer, Brachionus plicatilis. These Chlorella species were cultured in five different culture media; f/2, Erdschreiber, Complesal for marine species and S$\cdot$K, Wai and Complesal for freshwater species. Proximate analyses were done to see the protein, lipid and ash contents of a marine species, C. ellipsoidea and a freshwater species, C. vulgaris. Amino acids content of these species were also tested. C. ellipsoidea and C. vulgaris showed better growth than the other species. For marine Chlorella sp., f/2 media was better than Erdschreiber and Complesal. But for the freshwater species, Complesal showed the best result in growth. By the proximate analyses, C. ellipsoidea has higher lipid contents whereas C. vulgaris has higher protein and ash. In the analysis of amino acid of Chlorella, it was remarkable that freshwater Chlorella, C. vulgaris, has high content of $NH_3$ comparing with marine Chlorella, C, ellipsoidea. According to the above results, C. vulgaris seems have higher possibilities for mass culture of rotifer but further studies are needed.

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Physicochemical Properties of Hull-less Barley Flours Prepared with Different Grinding Mills (제분방법에 따른 쌀보리가루의 이화학적 특성)

  • Lee, Young-Tack;Seog, Ho-Moon;Cho, Mi-Kyung;Kim, Sung-Soo
    • Korean Journal of Food Science and Technology
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    • v.28 no.6
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    • pp.1078-1083
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    • 1996
  • During the pearling process of hull-less barley, protein, lipid, ash and insoluble dietary fiber (IDF) contents decreased, while soluble dietary fiber (SDF) and ${\beta}-glucan$ contents slightly increased. Depending on milling methods and types of grinding mills used, there were differences in particle size distribution of barley flour. Flour particle size was smaller in the following order of Fitz mill, Ball mill, Pin mill, Cyclotec sample mill and Jet mill. Color (brightness) was closely related to the particle size of barley flour. Damaged starch (%) in pearled barley flour was the highest in Jet mill among different mills. Flours prepared with Cyclone mill and Pin mill had a reasonable amount of damaged starch. Flour produced by Fitz mill showed the lowest amount of damaged starch. Scanning electron microscopy (SEM) of the flour samples demonstrated different sizes and shapes of particles consisting of starch granules and cell wall materials. Damaged starch tended to increase water absorption index (WAI), water solubility index (WSI), and water retention capacity (WRC). Pasting viscosity determined by amylograph was relatively high in Pin-milled and Cyclone-milled flours. Viscosity was the lowest in coarsely ground flour by Fits mill.

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Effects of Enrichment Planting with Five Native Species and Different Plantation Treatments on Seedling Growth Characteristics at Logged-over Forest in Lao PDR (라오스 개벌림에서 다섯가지 자생수종과 각기 다른 조림처리의 Enrichment Planting이 묘목의 생장특성에 미치는 효과)

  • Phongoudome, Chanhsamone;Sawathvong, Silavanh;Woo, Su-Young;Ho, Wai Mun;Park, Yeong-Dae
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.14 no.2
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    • pp.45-52
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    • 2012
  • Enrichment planting is commonly used for increasing the density of tree species in secondary forests and one of forest rehabilitation programs in Lao PDR. This study aimed to determine the performance of five native species in enrichment planting using different canopy opening treatments, and to examine the suitable species and silviculture techniques applicable to a logged-over forest in Lao PDR. Results of the study showed that only crown height had significant difference among species and planting treatments. The survival rate and crown diameter were significantly different by species, but not by planting treatments. However, root collar diameter (RCD) and height of seedlings showed significant differences among planting treatments. Among the study species, Hopea odorata and Dalbergia cochinchinensis showed significantly higher survival rate and height growth, indicating their suitability in enrichment planting for rehabilitation of forest in Lao PDR. The results also suggested that 2 m width line planting stimulated RCD growth, but wider line planting, such as gap planting, promoted height growth of seedlings.

Manufacturing of Hemp Seed Flake by Using Extrusion Process (압출성형공정을 이용한 삼 종자 후레이크 제조)

  • Tie, Jin;Gu, Bon-Jae;Ryu, Gi-Hyung
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.99-105
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    • 2010
  • Proximate composition of whole hemp seed, paste viscosity of whole hemp seed pellets, bulk density, color, compression force, water solubility and absorption index and bowl life of whole hemp seed flakes manufactured by extrusion process were determined to evaluate whole hemp seed as flake additive. Extrusion process conditions were 35% of moisture content and 90, 100 and 110$^{\circ}C$ of barrel temperature. Content of moisture content, crude protein, crude fat, crude ash from whole hemp seed were 5.67${\pm}$0.02%, 25.93${\pm}$0.16%, 28.21${\pm}$0.56% and 7.70${\pm}$0.08%, respectively. The pellets hemp seed at 100 or 110$^{\circ}C$ had higher paste viscosity as compared to those pellet at 90$^{\circ}C$. The bulk density values of all hemp seed flakes were between 0.24 to 0.43 g/mL, depending on the barrel temperatures. The hardness of flake increased with decreasing percentage of hemp seed in flakes. An increase in barrel temperature from 90$^{\circ}C$ to 110$^{\circ}C$ resulted in increase of WSI and decrease of WAI. The bowl life of hemp seed flakes was increased from 5.8 to 15.5 min with the decrease in percentage of hemp seed. The highest overall quality was showed in flake added with 20% of hemp seed.

Volumetric analysis of mucous retention cysts in the maxillary sinus: A retrospective study using cone-beam computed tomography

  • Hung, Kuofeng;Hui, Liuling;Yeung, Andy Wai Kan;Wu, Yiqun;Hsung, Richard Tai-Chiu;Bornstein, Michael M.
    • Imaging Science in Dentistry
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    • v.51 no.2
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    • pp.117-127
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    • 2021
  • Purpose: The aim of this study was to evaluate the volumetric characteristics of mucous retention cysts(MRCs) in the maxillary sinus and to analyze potential associations of MRCs with dentoalveolar pathologies. Materials and Methods: Cone-beam computed tomography (CBCT) scans exhibiting bilateral maxillary sinuses that were acquired from January 2016 to February 2019 were initially screened. A total of 227 scans(454 sinuses) that fulfilled the inclusion criteria were included. The presence, location, and volumetric characteristics of the diagnosed MRCs were evaluated on CBCT images using the 3D-Slicer software platform. The presence of MRCs was correlated with potential influencing factors including age, sex, and dentoalveolar pathology. For MRCs located on the sinus floor, factors with a potential impact on the volume, surface, and diameter were analyzed. Results: An MRC was present in 130 (28.6%) of the 454 sinuses. Most MRCs were located on the sinus walls and floor. The mean MRC volume, surface, and diameter were 551.21±1368.04 mm3, 228.09±437.56 mm2, and 9.63±5.40 mm, respectively. Significantly more sinuses with associated endodontically treated teeth/periapical lesions were diagnosed with an MRC located on the sinus floor. For MRCs located on the sinus floor, endodontic status exhibited a significant association with increased volume, surface, and diameter. Conclusion: Periapical lesions might be a contributing factor associated with the presence and volume of MRCs located on the sinus floor. The 3D-Slicer software platform was found to be a useful tool for clinicians to analyze the size of MRCs before surgical interventions such as sinus floor elevation procedures.

Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers (고추 세균성점무늬병 저항성 유전자원과 그 주요 특성)

  • Byeon, Si-Eun;Abebe, Alebel Mekuriaw;Jegal, Yoon-Hyuk;Wai, Khin Pa Pa;Siddique, Muhammad Irfan;Mo, Hwang-Sung;Yoo, Hee Ju;Jang, Kil-Su;Hwang, Ji-Eun;Jeon, Su-Gyeong;Lee, Su-Heon;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.779-789
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    • 2016
  • A total of 33 accessions of pepper (Capsicum spp.), including previously reported and newly discovered sources of resistance to bacterial spot caused by Xanthomonas euvesicatoria, were evaluated for their resistance to bacterial spot. The selected accessions were then grown and their horticultural characteristics were recorded. In a test for hypersensitive resistance (HR) to four races (P1, P3, P7, P8) of the pathogen found in Korea, KC00939 and Chilbok No.2, which carry the Bs2 gene, exhibited a hypersensitive response to all four races, as expected. Chilbok No.3, which carries the Bs3 gene, showed a hypersensitive reaction to race 1 and 7, as expected. KC00939 exhibited a high ASTA color value and tolerance to multiple infections from a viral complex of Cucumber mosaic virus (CMV) and Broad bean wilt virus (BBWV). Thus, this accession represents a promising genetic resource for breeding cultivars with multiple disease resistance and strong red coloration. KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127, KC01704, and KC00995 did not exhibit hypersensitivity but showed a high level of general resistance when evaluated by spray inoculation. KC01617, KC01760, KC01779, KC01137, KC01704, and KC01777 are newly identified sources of resistance to bacterial spot. The previously and newly identified sources of resistance to bacterial spot evaluated in this study, including information about their resistance to CMV and BBWV complex in the field, the contents of pungent and sweet taste components, and the color values of dry fruits, will be useful for breeding pepper cultivars with resistance to bacterial spot.