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Occurrence and diet analysis of sea turtles in Korean shore

  • Kim, Jihee;Kim, Il-Hun;Kim, Min-Seop;Lee, Hae Rim;Kim, Young Jun;Park, Sangkyu;Yang, Dongwoo
    • Journal of Ecology and Environment
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    • v.45 no.4
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    • pp.203-217
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    • 2021
  • Background: Sea turtles, which are globally endangered species, have been stranded and found as bycatch on the Korean shore recently. More studies on sea turtles in Korea are necessary to aid their conservation. To investigate the spatio-temporal occurrence patterns of sea turtles on the Korean shore, we recorded sampling locations and dates, identified species and sexes and measured sizes (maximum curved carapace length; CCL) of collected sea turtles from the year 2014 to 2020. For an analysis of diets through stomach contents, we identified the morphology of the remaining food and extracted DNA, followed by amplification, cloning, and sequencing. Results: A total of 62 stranded or bycaught sea turtle samples were collected from the Korean shores during the study period. There were 36 loggerhead turtles, which were the dominant species, followed by 19 green turtles, three hawksbill turtles, two olive ridley turtles, and two leatherback turtles. The highest numbers were collected in the year 2017 and during summer among the seasons. In terms of locations, most sea turtles were collected from the East Sea, especially from Pohang. Comparing the sizes of collected sea turtles according to species, the average CCL of loggerhead turtles was 79.8 cm, of green turtles was 73.5 cm, and of the relatively large leatherback turtle species was 126.2 cm. In most species, the proportion of females was higher than that of males and juveniles, and was more than 70% across all the species. Food remains were morphologically identified from 19 stomachs, mainly at class level. Seaweeds were abundant in stomachs of green turtles, and Bivalvia was the most detected food item in loggerhead turtles. Based on DNA analysis, food items from a total of 26 stomachs were identified to the species or genus level. The gulfweed, Sargassum thunbergii, and the kelp species, Saccharina japonica, were frequently detected from the stomachs of green turtles and the jellyfish, Cyanea nozakii, the swimming crab, Portunus trituberculatus, and kelps had high frequencies of occurrences in loggerhead turtles. Conclusions: Our findings support those of previous studies suggesting that sea turtles are steadily appearing in the Korean sea. In addition, we verified that fish and seaweed, which inhabit the Korean sea, are frequently detected in the stomach of sea turtles. Accordingly, there is a possibility that sea turtles use the Korean sea as feeding grounds and habitats. These results can serve as basic data for the conservation of globally endangered sea turtles.

Preliminary Results of Marine Heat Flow Measurements in the Chukchi Abyssal Plain, Arctic Ocean, and Constraints on Crustal Origin (북극 척치 해저평원의 해양지열관측 초기결과와 지각기원에 대한 의미)

  • Kim, Young-Gyun;Hong, Jong Kuk;Jin, Young Keun;Jang, Minseok;So, Byung Dal
    • The Journal of Engineering Geology
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    • v.32 no.1
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    • pp.113-126
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    • 2022
  • The tectonic history of the Chukchi Abyssal Plain in the Amerasia Basin, Arctic Ocean, has not been fully explored due to the harsh conditions of sea ice preventing detailed observation. Existing models of the tectonic history of the region provide contrasting interpretation of the timing of formation of the crust (Mesozoic to Cenozoic), crust type (from hyper-extended continental crust to oceanic crust), and formation process (from parallel/fan-shaped rifting to transformation faulting). To help determine the age of the oceanic crust, the geothermal gradient was measured at three stations in the south of abyssal plain at depth of 2,160-2,250 m below sea level. Heat flow measurement stations were located perpendicular to the spreading axis over a 40 km-long transect. In-situ thermal conductivity measurement, corrected by the laboratory test, gave observed marine heat flows of 55 to 61 mW/m2. All measurements were taken during Arctic expeditions in 2018 (ARA09C expedition) and 2021 (ARA12C expedition) by the Korean ice-breaking research vessel (IBRV) Araon. Given the assumption of oceanic crust, the results correspond to formation in the Late Cretaceous (Mesozoic). The inferred age supports the hypothesis of formation activated by the opening of the Makarov Basin during the Late Mesozoic-Cenozoic. This would make it contemporaneous with rifting of the Chukchi Border Land immediately east of the abyssal plain. The heat flow data indicate the base of the gas hydrate stability zone is located 332-367 m below the seafloor, this will help to identify the gas hydrate-related bottom simulating reflector in the future seismic survey, as already identified on the Chukchi Plateau. Further geophysical surveys, including heat flow measurements, are required to increase our understanding of the formation process and thermal mantle structure of the abyssal plain.

Studies on the Inheritance of Agronomic Characteristics in Upland Cotton Varieties (Gossypium hirsutum L.) in Korea (육지면품종의 유용형질의 유전에 관한 연구)

  • Bang-Myung Kae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.21 no.2
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    • pp.281-313
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    • 1976
  • To obtain fundamental informations on cotton breeding efficiences for Korea, individual genetic relationships and interrelationships between the agronomic characteristics of Upland cotton were investigated. These experiments were couducted at the Mokpo Branch Station $(34^{\circ}48'N, $ $126^{\circ}23'E$ and altitude of 10m above sea level) from 1969 through 1972. Heterosis, combining ability, dominance and recessive gene action, genetic variance, and phenotypic and genotypic correlation were investigated by $F_1'S$ from an 11-parent partial diallel cross and the segregating $F_2$ and $F_3$ populations of the cross Paymaster times Heujueusseo Trice. The following points resulted from this study, 1. Heteroses for number of bolls per plant and lint yield were significant at 27, 84% and 37.26%, respectively. No other character had significant heteroses. 2. The GCA estimates for all studied characteristics were higher than the SCA estimates. Varieties with high GCA effects were Suwon 1 for earliness, Paymaster and Arijona for high lint percent, and Arijona for long fiber, etc, 3. SCA estimates for lint yield varied widely in crosses with Mokpo 4, Mokpo 6 and Heujueusseo Trice. Those crosses with the highest SCA effects were combinations with large characteristics differences, Example of these crosses are Mokpo 4 times Acala 1517W, Mokpo 4 times D. P. L. and Heujueusseo Trice aud Paymaster. 4. Early-maturing varieties were completely dominant to late-maturing varieties in some combinations while other crosses gave intermediate phenotypes. These results suggest additive genetic action by multi-genes. Heujueusseo Trice, Mokpo 6, and Suwon 1 showed highest degree of dominance for earliness. 5. There were no significant trends for inheritance of weight of boll and 100 seeds weight. 6. Long staple was partially to completely dominant to short staple. Though there were single gene ratios the rate of dominance decreased in the $F_2$ and $F_3$ populations in the cross between the long staple variety Paymaster and the short staple variety Heujueusseo Trice. Diallel cross $F_1$ hybrids showed complicated allelic gene action for staple length. Various dominance degree were shown by varieties. 7. Number of bolls per plant indicated strong over-dominance and small non-allelic additive gene action. 8. Lint Yield was characterized by over-dominance and by multiple non-allelic-gene action. High-yielding varieties were dominant to low-yielding ones. However, the low-yielding variety Heujueusseo Trice showed over-dominance, indicating different reactions according to the varieties and combinations. 9. Broad sense heritability for days to flowering was 34-39% while narrow sense heritability was 11%. Large variations of individual plants caused by Korean climatic conditions cause this situation. Heritability estimates for weight of boll was 30% for broad sense and 22% for narrow sense. 10. Heritability estimates for staple length and lint percent were very high suggesting strong selection effects. 11. Narrow sense heritability estimates for number of bolls per plant was 30% in the diallel cross $F_1$ hybrids and 36% in the $F_2$ population of the special cross. Broad sense heritability was estimated at 67% suggesting that. 12. Heritability estimates for lint yield was low due to high over-dominance in the diallel cross $F_1$ hybrids. Heritability estimates for yield was low in the $F_1$ hybrids but high in the $F_2$ and $F_3$ populations. 13. Phenotypic and genotypic correlations between lint percent and days to flowering and between staple length and days to flowering were high in the $F_1, $ $F_2$ and $F_3$ populations. Late-maturing varieties and individuals had long staple and high lint percent in general. As the correlation between days to flowering and lint yield was extremely low, the two traits were considered independent of each other. Days to flowering and number of bolls per plant were negatively correlated in the $F_3$ population, indicating early-maturing individual plants with many bolls may be readily selected. 14. Phenotypic and genotypic correlations between lint percent and staple length were high in $F_1, $ $F_2$ and $F_3$ populations. Accordingly, long staple varieties were high in lint percent. It was recognized that lint yield and lint percent were positively correlated in the diallel cross $F_1$ hybrids, and lint percent and staple length were positively correlated in the $F_2$ population, indicating that lint percent and staple length affect lint yield. 15. Lint yield was significantly and positively phenotypically correlated with number of bolls per plant in $F_1, $ $F_2$ and $F_3$ populations. A high genotypic correlation was also noted indicating a close genetic relationship. The selection efficiencies for a high-yielding variety can be increased when individual plants with many bolls are selected in later generations. The selection efficiencies for good fiber quality can be enhanced when individuals with long staple and high lint percent are selected in early generations.

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Estimation of Breed and Environmental Effects on Economic Traits of Performance-Tested Pigs (검정소 검정돈의 품종 및 환경요인의 효과 추정)

  • Park, J.W.;Kim, B.W.;Kim, H.C.;Lee, K.W.;Choi, C.S.;Kang, W.G.;Hong, S.K.;Ha, J.K.;Jeon, J.T.;Lee, J.G.
    • Journal of Animal Science and Technology
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    • v.45 no.6
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    • pp.923-932
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    • 2003
  • This study was carried out to estimate the effects of breed and environment such as sex, test station, test year, test season, parity, initial and final weight on average daily gain, age at 90kg, backfat thickness, feed efficiency, lean percent and selection index on the basis of the performance data collected from 25,790 pigs of Duroc, Yorkshire and Landrace breeds which were performance-tested at the Korea Swine Testing Station from 1991 to 2002. The results obtained in the study are summarized as follows; 1. The means of the major economic traits were estimated as 959.95${\pm}$0.699g for average daily gain, 138.36${\pm}$0.072days for age at 90kg, 1.41${\pm}$0.001cm for backfat thickness, 2.33${\pm}$0.001 for feed efficiency, 56.71${\pm}$0.018% for lean percent and 221.65${\pm}$0.113 for selection index. 2. The effect of breed was statistically significant for all studied traits. Briefly, Duroc showed the best performance for the average daily gain and age at 90kg. Landrace had the best performances for the backfat thickness and lean meat percent. In feed efficiency and selection index, Yorkshire had a better score than other breeds. 3. The least-squares means of female and male for the traits studied were 923.05${\pm}$1.289g and 974.53${\pm}$0.856g for average daily gain, 139.74${\pm}$0.145days and 137.21${\pm}$0.097days for age at 90kg, 1.49${\pm}$0.002cm and 1.39${\pm}$0.002cm for backfat thickness, 2.43${\pm}$0.002 and 2.28${\pm}$0.002 for feed efficiency, 56.43${\pm}$0.034% and 56.81${\pm}$0.023% for lean percent and 211.37${\pm}$0.194 and 224.61${\pm}$0.129 for selection index. Therefore, males were superior to females for all traits examined. 4. The effect of test station was statistically significant for all traits except for selection index. Performances for age at 90kg, backfat thickness, feed efficiency and lean meat percent collected from Test station 2 were higher than those from Test station 1. However, Test station 1 showed better average daily gain. 5. The initial weight and final weight included as a covariate in this study had a significant influence on average daily gain, age at 90kg, backfat thickness, feed efficiency and selection index. From the absolute values of the estimated regression coefficients, it was inferred that the final weight had greater effect for the investigated traits than the initial weight.

Estimation of Parameters for Individual Growth Curves of Cows in Bostaurus Coreanae (한우 암소의 개체별 성장곡선 모수 추정)

  • Lee, C.W.;Choi, J.G.;Jeon, G.J.;Na, K.J.;Lee, C.;Hwang, J.M.;Kim, B.W.;Kim, J.B.
    • Journal of Animal Science and Technology
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    • v.45 no.5
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    • pp.689-694
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    • 2003
  • Weight records of Hanwoo cows from birth to 36 months of age collected in Daekwanryeong branch, National Livestock Research Institute(NLRI) were fitted to Gompertz, von Bertalanffy and Logistic functions. For the growth curve parameters fitted on individual records using Gompertz model, the mean estimates of mature weight(A), growth ratio(b) and growth rate(k) were 383.42 ${\pm}$ 97.29kg, 2.374 ${\pm}$ 0.340 and 0.0037 ${\pm}$ 0.0012, respectively, and mean estimates of body weight, age and daily gain rate at inflection were 141.05 ${\pm}$ 35.79kg, 255.63 ${\pm}$ 109.09 day and 0.500 ${\pm}$ 0.123kg, respectively. For von BertalanfTy model, the mean estimates of A, b and k were 410.47 ${\pm}$ 117.98kg, 0.575${\pm}$0.057 and 0.003 ${\pm}$ 0.001, and mean estimates of body weight, age and daily gain at inflection were 121.62 ${\pm}$ 34.94kg, 211.02 ${\pm}$ 105.53 and 0.504 ${\pm}$ O.l24kg. For Logistic model, the mean estimates of A, b and k were 347.64 ${\pm}$ 97.29kg, 6.73 ${\pm}$ 0.34 and 0.006 ${\pm}$ 0.0018, and mean estimates of body weight, age and daily gain at inflection were 173.82 ${\pm}$ 37.25kg, 324.47 ${\pm}$ 126.85 and 0.508 ${\pm}$ 0.131kg. Coefficients of variation for the A, b and k parameter estimates were 25.3%, 14.3% and 32.4%, respectively, for Gompertz model, 28.70/0, 9.9% and 33.3% for von Bertalanffy model, and 27.9°/0, 5.0% and 30.0% for Logistic model.

An evaluation of the adequacy of pont's index (Pont 지수의 임상적 적합성에 대한 평가)

  • Kim, Seong-Hun;Lee, Ki-Soo
    • The korean journal of orthodontics
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    • v.30 no.1 s.78
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    • pp.115-126
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    • 2000
  • Dental arch expansion is one of the method used to solve the dental crowding problem by non-extraction. Many formulae using tooth size have been suggested to predict ideal inter-premolar and inter-molar width. The purpose of this study was to evaluate the adequacy of some upper dental arch width prediction methods, namely Pont's method, Schmuth's method and Cha's method. The sample consisted of the casts of 119 Korean young adults who had no muscular abnormality, no skeletal discrepancy, and Angle's Class I molar relationships. Measurements were obtained directly from plaster casts; they Included mesiodistal crown diameters of the four maxillary incisors, as well as maxillary inter-first-premolar and inter-first-molar arch widths as specified by Pont. The correlation coefficients between the sum of incisors(SI) and upper dental arch width were calculated. The differences between predicted width and actual width were classified as overestimated, properestimated, and underestimated. The data obtained from each group were analyzed for statistical differences. The results were as follows : 1. Upper dental arch width indices were calculated from SI in normal occlusion (81.96 : premolar index, 62.55 : molar index). 2. Low correlations between SI and arch width were noted in normal occlusion (0.50 in the inter-premolar width, 0.39 in the inter-molar width). 3. Pont's formula and Schmuth's formula tended to overestimate the inter-premolar width. A more even distribution of estimates was noted in Cha's fomula. 4. Cases within $\pm$1 mm range of observed inter-premolar width were $45\%$ in the Cha's formula, $40\%$ in the Pont's formula, and $39\%$ in the Schmuth's formula. 5. All formulae had a tendency to underestimate the inter-molar width, but Cha's formula had better predictability than others. 6. Cases within $\pm$1 mm range of observed inter-molar width were $40\%$ in the Cha's formula, $29\%$ in the Pont's formula, and $13\%$ of Schmuth's formula. The data presented in this study does not support the clinical usefulness of ideal arch width prediction methods using the mesiodistal width of maxillary incisors.

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Health Status of Primary School Children in a Part of Remote Rural Area (일부(一部) 벽지농촌(僻地農村) 학동(學童)들의 건강실태(健康實態))

  • Park, Won-Kihl
    • Journal of Preventive Medicine and Public Health
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    • v.7 no.1
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    • pp.211-222
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    • 1974
  • The survey was carried out on 1,031 primary school children for about 1 month, from June 1, to June 30, 1974, for the purose of understanding indirectly scio-medical status of a remote rural area, reflecting health status of primary school children. The summarized results were as follows: 1) The average age of surveyed children according to school year by sex was older in girls than in boys. The entrance in school at suitable age was peaked as 80.9% in boys of 5th school year and 72.4% in girls of 3rd school year, and also sunk 68.8% in boys of 2nd school year and 58.7% in girls of 1st school year. 2) Prevalence rate according to W.H.O. diseases classification during last a month and a year. a) During last month: The diseases of respiratory system remarked the highest 101.1 in boys and 116.6 in girls. The next were diseases of digestive system (24.2 in boys and 32.1 in girls), The girls were higher than boys in prevalence rate. b) During last year: The diseases of diegestive system ranked the highest 133.0 in boys and next, the diseases of respiratory system (108.6 in boys). c) Present illness: Number of code III ranked the highest 129.2 and next XII(127.3) in boys but inverted in girls such as XII(144.9) and III(116.7) and also, Number of code XVII was prevalenced twice in boys than in girls. d) Chief complaints by sex: Abdominal pain ranked the highest 71.2 per 1,000 population and next headache (34.7) in boys but headache ranked the heighest 88.5 and next abdominal pain (63.3) in girls. e) Water drinking habit: Number of code IX was higher in habit of raw water drinker than in habit of boiled water drinker and healthy children were higher in boiled water habit group than in raw water habit group. The diseases of respiratory system were the highest 124.74 in girls and next, the diseases of digestive system (52.3 in girls). According to school year, healthy children were increased to higher grades. 3) Average sick day per total surveyed children and patient during last month and last year. a) Per surveyed 1,031 children was 0.28 days during last month and 0.98 days during last year. And also per patient was 1.78 days during last month and 3.22 days during last year. b) Average sick day was higher in girls than in boys during last month, but inverted during last year. 4) Average absented day per total surveyed children and patient during last month and last year. a) Per surveyed 1,031 children were 0.43 days during last month and 3.81 days during last year (omitted 1st school year). b) Per absented children were 2.0 days during last month and 7.10 days during last year. c) Per absented children were 8.16 days in boys and 3.17 days in girls. 5) Utilization of medical facilities: The drug-store was consulted 91.2% of the total utilized boys and girls. The strengthening of medical facilities and medical care activities in health subcenter is urgently required 6) Medical Expenses by period and sex: a) Average medical expenses per surveyed 1,031 children were 35.95 won, per patient were 298.04 won during last month. b) Average medical expenses per survey children (omitted 1st school year) were 80.56 won, per patient were 243.98 won and per treated patient were 318.87 won during last year. c) Medical expenses were higher in boys than in girls. 7) Rohrer index by sex, present illness and school year. a) Average Rohrer index was 129.8 in boys and 126.2 in girls. b) Average Rohrer index was increased for 1st school year to 2nd school year and thereafter falling down step by step by school year. c) Under 151-160 Rohrer index number of code III was the highest and above 151-160 Rohrer index of code XII was peaked.

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Cryopreservation of Mouse IVF Zygotes by Vitrification (체외수정된 생쥐 1-세포기 배의 초자화 동결)

  • 김묘경;이현숙;엄상준;김은영;윤산현;박세필;정길생;임진호
    • Korean Journal of Animal Reproduction
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    • v.20 no.2
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    • pp.119-126
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    • 1996
  • This study was carried out to determine the optimal condition for successful and efficient c cryopreservation of zygotes, 1-cell embryos, using EFS40 which was 40% (v/v) ethylene glycol diluted in DPBS medium containing 30% Fic-oll (w/v) and 0.3 M sucrose. After mouse zygote produced by IVF was vitrified by two freezing methods, the post-warming survival rates of 1-cell zygotes were assessed as cleavage to the 2-cell stage and development into the hatching blastocysts at 5 day. In the one-step method, when embryos were directly exposed to the vitrification solution at 25$^{\circ}C$ for 1 min., survival and development rates of zygotes were 85.5% and 31.9% In the two-step method, embryos were equilibrated with a dilute 20% EG for 1, 3, 5 min. before 1 min. exposure to EFS40, re-spectively. However, the rates of development (17.7, 3.3, 0%) were lower than that of one-step method. The highest survival rate (95.9%) was obtained by one-step method which exposes embryos in EFS40 for 30 sec. In this condition, 63. 8% of cleaved 2-cell developed into hatching blastocysts. In the cell number of Total and ICM using differential labelling technique, there are no significant differences in the cell number of Total and ICM between blastocysts devel oped in vitrified-thawed embryos (63.2${\pm}$16.9, 1 13.5${\pm}$4.0) and control balstocysts (54.0${\pm}$15.2, 1 12.3${\pm}$4.6). Therefore, these results show that mouse zygotes can be successfully cryopreserved by a simple vitrification method although developmental rates of vitrified embryos were reduced. In conclusion, this proposed vitrifi cation procedures can be useful in the cryopreservation of mouse IVF zygotes.

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Comparison between REML and Bayesian via Gibbs Sampling Algorithm with a Mixed Animal Model to Estimate Genetic Parameters for Carcass Traits in Hanwoo(Korean Native Cattle) (한우의 도체형질 유전모수 추정을 위한 REML과 Bayesian via Gibbs Sampling 방법의 비교 연구)

  • Roh, S.H.;Kim, B.W.;Kim, H.S.;Min, H.S.;Yoon, H.B.;Lee, D.H.;Jeon, J.T.;Lee, J.G.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.719-728
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    • 2004
  • The aims of this study were to estimate genetic parameters for carcass traits on Hanwoo(Korean Native Cattle) and to compare two different statistical algorithms for estimating genetic parameters. Data obtained from 1526 steers at Hanwoo Improvement Center and Hanwoo Improvement Complex Area from 1996 to 2001 were used for the analyses. The carcass traits considered in these studies were carcass weight, dressing percent, eye muscle area, backfat thickness, and marbling score. Estimated genetic parameters using EM-REML algorithm were compared to those by Bayesian inference via Gibbs Sampling to find out statistical properties. The estimated heritabilities of carcass traits by REML method were 0.28, 0.25, 0.35, 0.39 and 0.51, respectively and those by Gibbs Sampling method were 0.29, 0.25, 0.40, 0.42 and 0.54, respectively. This estimates were not significantly different, even though the estimated heritabilities by Gibbs Sampling method were higher than ones by REML method. Since the estimated statistics by REML method and Gibbs Sampling method were not significantly different in this study, it is inferred that both mothods could be efficiently applied for the analysis of carcass traits of cattle. However, further studies are demanded to define an optimal statistical method for handling large scale performance data.

Rapid prenatal diagnosis of Down syndrome and Edward syndrome by fluorescence In situ hybridization : Clinical experience with 309 cases (FISH를 이용한 다운증후군과 에드워드증후군의 신속한 산전확인 : 309예의 임상적 고찰)

  • Kang, Jin-Hee;Lee, Sook-Hwan;Park, Sang-Hee;Park, Ji-Hyun;Kim, Ji-Youn;Han, Won-Bo;Kim, In-Hyun;Park, Sang-Won;Jang, Jin-Beum;Lee, Kyoung-Jin;Park, Hee-Jin;Jun, Hye-Sun;Lee, Kyung-Ju;Shin, Joong-Sik;Cha, Dong-Hyun
    • Journal of Genetic Medicine
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    • v.4 no.1
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    • pp.64-71
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    • 2007
  • Purpose : The purpose of this study was to evaluate the clinical utility of rapid detection of Down syndrome and Edward syndrome by Interphase Fluorescence in Situ Hybridization (FISH) analysis. Methods : Aretrospective study in 309 cases of amniotic fluid samples, analysed by interphase FISH with DNA probes specific to chromosome 18 and 21, was performed. All FISH results w ere compared with conventional cytogenetic karyotypings. Results : The results were considered as informative and they were obtained within 48 hrs. A case of Down syndrome and a case of Edward syndrome were diagnosed by FISH and confirmed by subsequent cytogenetic analysis. In 12 cases with normal FISH results, the cytogenetic analysis showed a case of partial trisomy 22, three cases of sex chromosomal aneuploidy, two cases of mosaicism, two cases of microdeletion, and four cases of structural rearrangement. Conclusion : FISH is a rapid and effective diagnostic method, which can be used as an adjunctive test to cytogenetic analysis, for prenatal identification of chromosome aneuploidies. For the more genome-wide screening with variety of probes, the technique of FISH is both expensive and labor-intensive.

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