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Effects of Butachlor on the Cell Division and Protein Synthesis on Oat(Avena sativa L.) (Butachlor가 귀리의 세포분열(細胞分裂) 및 단백질(蛋白質) 합성(合成)에 미치는 영향(影響))

  • Kwon, S.W.;Kim, J.C.
    • Korean Journal of Weed Science
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    • v.9 no.3
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    • pp.245-249
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    • 1989
  • The effects of varying concentrations and durations of butachlor [N-(bytoxymety 1,)-2-chlor -2, 6-diethy lacetanilide treatment on oat(Avena sativa L.) root cell division and protein synthesis were studied. The highest concentration ($1{\times}10^{-3}M$) of butachlor caused the significant inhibition of cell division after 18hrs treatment. After 18hrs treatment, 59% and 82% inhibition of cell division occurred at $1{\times}10^{-4}M$ and $1{\times}10^{-3}M$, respectively, while 9% inhibition of cell division did at $1{\times}10^{-6}M$ concentration at the same exposure period. To investigate protein synthesis, the oats were treated for 18 and 24hrs with concentrations ranging from $1{\times}10^{-6}M$ to $1{\times}10^{-3}M$ butachlor. After 18hrs, butachlor treatment of oat with $1{\times}10^{-4}M$ inhibitited 23% protein synthesis, and butachlor treatment with $1{\times}10^{-4}M$ caused 34% inhibition after 24hrs. With SDS-PAGE of proteins extracted from oat root tips, butachlor usually inhibited the 16, 18, 30, 43 and 43.5 kD polypeptide, and proteins of root tips are made up of subunits below 100 kD polypeptide.

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Effect of Several Herbicides on Weed Control and Seedling Growth in Rice Nursery (수도(水稻)못자리에 있어서 몇가지 제초제처리(除草劑處理)가 잡초방제(雜草防除) 및 묘생육(苗生育)에 미치는 영향(影響))

  • Ree, D.W.;Hong, Y.K.;Kim, J.C.;Kim, H.D.
    • Korean Journal of Weed Science
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    • v.4 no.2
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    • pp.179-187
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    • 1984
  • This experiment was carried out to clarify the effect of herbicide chlormethoxynil 7G, CNP 9G and butachlor 3.5/pyrazolate 6G on weed control and seedling growth in rice seedbed. Different quantities of the herbicides were applied as soil incorporation or surface broadcasting. At earlier sowing annual grasses such as Echinochlva spp. and Alopecurus spp. were dominated, but Cyperus spp. were dominated at later sowing. Weed suppression by herbicides was greater in soil incorporation than in broadcasting and chlormethoxynil 7G and CNP 9G application (30kg/ha) showed about 70% weed control, while butachlor 3.5/pyrazolate 6G (10-20kg/ha) showed 90 to 100% weed control. At lower rate of butachlor 3.5/pyrazolate 6G (10kg/ha) emergence ratio, healthy seedling ratio and relative growth rate of rice were higher, and phytotoxicity was lower than in the others.

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Studies on the productivoty of the Native Reed ( Phragmites communis Trinius ) II. Effect of fertilizer application on the productivity of the native reed during the period of vegetation (갈대의 생산력에 관한 연구 II. 시비가 생육시기별 갈대의 생산성에 미치는 영향)

  • Chun, W.B.;Yoon, C.;Rho, S.H.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.6 no.1
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    • pp.24-30
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    • 1986
  • This experiment was carried out in order to study the effect of fertilizer application and seasonal changes on the productivity of the native reed (Phragmites communis Trinius) on the reclaimed tidal flate in Chonnam province. The samples of reed were taken at about 30-days interval from May to October, 1982 and evaluated plant height, blade & sheath-stem ratio, grass yield, the feed compositions and in vitro dry matter digestibility (IVD). The results are summarized as follows: 1. Plant height, grass yield, crude protein content and in vitro dry matter digestibility of the reed were significantly increased by fertilizer application, and ADF content was significantly decreased. 2. According to the significance test of coefficience, there was a significant negative correlation (P<0.05) between in vitro dry matter digestibility and plant height, grass yield, and the content of crude fiber and ADF, but a positive correlation (P<0.05) between in vitro dry matter digestibility and blade & sheath-stem ratio, and the content of crude protein and crude fat.

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Effect of Heat Treatments on Physical Properties and in vitro Glucose, Bile Acid, and Cadmium Transport Retardation of Wax Gourd (Benincasa hispida) (동아의 물리적 특성 및 in vitro 포도당, 담즙산, 카드뮴 투과억제 효과에 대한 열처리 영향)

  • Ju, In-Ok;Jung, Gi-Tai;Ryu, Jeong;Kim, Young-Soo
    • Korean Journal of Food Science and Technology
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    • v.35 no.6
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    • pp.1117-1123
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    • 2003
  • The effects of heat treatment on the physical and physical and physiological properties of wax gourd (Benincasa hispida) were examined. The applied heat treatments were autoclaved at $121^{\circ}C$ for 1 hr, boiled for 30 min, and microwaved at 680 W for 5 min. The water retention capacity (WRC) of the wax gourds was 9.43 g/g for the microwaved samples, 5.12 g/g for the boiled samples, 4.63 g/g for the raw samples, and 2.61 g/g for the autoclaved samples. Heat treatment caused to increase swelling by up to $4.4{\sim}7.8\;mL/g$. Calcium binding capacity of heat-treated wax gourd increased in the order of microwaved, boiled, raw, autoclaved samples. Scanning electron microscopy (SME) showed that autoclaving caused the most severe structural modifications, while microwave treatment produced the least modifications. The retarding effect on glucose and bile acid transport depended on the heat treatment. Only boiling showed the glucose retardation effect. Bile acid retardation effect increased in order of boiling (22.9%), autoclaving (17.1%), microwave treatment (14.3%), and raw wax gourd (8.6%). The cadmium retardation effect was significantly high in all samples.

Effect of external-phosphorus supply on the phosphorus status of soybean nodules and the P-uptake system of isolated bacteroids (인산공급이 대두근류의 인산형태와 bacteroid의 인산흡수에 미치는 영향)

  • Sa, Tong-Min;Israel, Daniel W.
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.117-124
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    • 1991
  • Soybean plants inoculated with Bradyrhizobium japonicum MN 110 were supplied with nutrient solutions containing 1.0, 0.25 and 0.5.nM-P to characterize the effect of externaI-P supply on the phosphorus status of nodules and on the P-uptake system of isolated bacteroids from nodules. After 48 days of growth, whole plant dry mass in the 0.25 and 0.05 mM-P treatments decreased significantly. The Pi concentrations in nodules were 4.1, 2.5 and 2.0 mM for 1.0, 0.25 and 0.05 mM-P treatments, respectively. The external-P supply did not significantly affect the distribution of phosphorus among inorganic phosphate(Pi), soluble organic-phosphorus(SOP) and insoluble organic-phosphorus(TOP) fractions in nodules. The Pi concentrations in young leaves of 0.25 and 0.05 mM-P plants were 33% and 20% , respectively, of those in young leaves of 1.0 mM-P plants and Pi concentrations in old leaves were only 16% and 7%, respectively, of those in old leaves of 1.0 mM-P plants. Phosphorus deficiency decreased the percentage of total leaf phosphorus in the Pi fraction and increased the percentage of total leaf phosphorus in the IOP fraction. The bacteroid number ranged from 0.87 to $1.30{\times}10^{11}$ Per GFW nodule regardless of external-P supply to the host Plants and Plant age, The P-uptake rates were the same (15-16 pmoles /min./$10^8$ bacteroids) for the bacteroids isolated from nodules of 1.0 mM-P and 0.05 mM-P plants. These results indicate that Pi concentrations in nodules of phosphorus-deficient plants are sufficient for proliferation of bacteroids and that the P-uptake system of bacteroids is in a repressed state even when host plant growth is severely restricted by phosphorus-deficiency stress.

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Effect of CIDR on Estrus Synchronization and Artificial Insemination of Korean Native Goat (CIDR를 이용한 재래 흑염소의 발정동기화와 인공수정)

  • Choe C. Y.;Kang D. W.;Cho S. R.;Kim H. J.;Choi S. H.;Choi S. H.;Kim Y. K;Rho G. J.;Choe S. Y.;Son D. S.
    • Journal of Embryo Transfer
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    • v.20 no.3
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    • pp.297-302
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    • 2005
  • Artificial insemination (AI) technology has widely applied in domestic animals including cattle for production and preservation of valuable animals. However, AI in goat was poorly studied. We here compared two different estrus synchronization methods, CIDR (controlled intravaginal drug release)+$PGF_2a$ and CIDR+PMSG, in Korean native goats. After treatment of CIDR+$PGF_2a$ or CIDR+PMSG for estrus synchronization, AI was applied to 38 recipients. Of 18 recipients treated with CIDR+$PGF_2a$, 4 recipients produced offsprings. However, no pregnancy was obtained in 20 recipients treated with CIDR+PMSG. Especially, after treatment of CIDR+$PGF_2a$, AI with fresh or frozen sperm showed markedly higher parturition rates than that of CIDR+PMSG. From these results, we suggest that combination of CIDR and $PGF_2a$ could be used as good method for estrus synchronization in Korean native goats.

Preparation of Fe4[Fe(CN)6]3 Coated on Mica or TiO2/Mica for Infrared Reflective Blue Pigments and Isolation-heat Properties of These Paints (Fe4[Fe(CN)6]3가 코팅된 Mica 또는 TiO2/Mica 적외선 반사용 청색안료 제조 및 이 도료의 차열 특성 평가)

  • Jung, Ha-Young;Kim, Dae Sung;Lee, Hyun-Jin;Lee, Seung-Ho;Lim, Hyung Mi;Choi, Byung-Ki;Kang, Kwang-Jung;Choi, Jin-Sub
    • Korean Journal of Materials Research
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    • v.23 no.12
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    • pp.672-679
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    • 2013
  • $Fe_4[Fe(CN)_6]_3$ coated on a mica or $TiO_2$/mica surface as infrared reflective blue pigment was prepared by a hydrothermal method. $Fe_4[Fe(CN)_6]_3$, used as coloring agent, was uniformly coated on mica or $TiO_2$/mica under the optimized condition of a 1.2 : 1 weight ratio between iron(III) chloride hexahydrate and potassium ferrocyanidetrihydrate at the initial pH level of 4.5 at $70^{\circ}C$. The infrared (IR)-reflective pigments were characterized by SEM, Zeta-potenial, FT-IR, and UV-VIS NIR spectrophotometry. Especially the CIE color coordinate and total solar reflectance(TSR) properties of the pigments were investigated in relation to variation of the coating and coated substrate thicknesses. Isolation-heat paint was prepared with 20 wt% blue pigments fully dispersed in acryl-urethane resin and several additives to coat the film uniformly. The films were also measured with CIE color coordinate, TSR, and the surface temperature was recorded by an isolation-heat measuring system. The pigments and films of $Fe_4[Fe(CN)_6]_3$ coated on mica and $TiO_2$/mica showed high TSR values compared with the TSR value of $Fe_4[Fe(CN)_6]_3$ itself. According to the increase of TSR value, the property of isolation-heat is effective. To realize the optimal blue color, we applied the the pigment to $TiO_2$ coated mica(TM(b)) which has blueish interference color. The pigment of $Fe_4[Fe(CN)_6]_3$ coated on TM(b) shows a strong blue color compared with that of $Fe_4[Fe(CN)_6]_3$ coated on $TiO_2$/Mmca(TM(w)), which has a whitish interference color.

Effects of the Nuegra from Male Silkworm Extract on Enhancement of the Masculine Function and Activation of Overall Physical Function

  • Kim, D. C.;Kim, Y. W.;Park, M. S.;J. K. Suh;Lee, D. S.;Lee, S. H.;B. H. Chun;Y. K. Jun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.5 no.1
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    • pp.109-122
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    • 2002
  • The purpose of this investigation is to evaluate the effects of the Nuegra on enhancement of the masculine and physical activities in general through measuring changes of the testosterone, FSH and subjective symptoms like fatigue, insomnia, urinary stream, muscular weakness, libido and erectile dysfunction. Total 168 male subjects were enrolled from 12 urology, internal medicine clinics and general practitioner, During the 6-week investigational period, 2 capsules of Nuegra were given to the subjects right after meal for 4 weeks, and 1 capsule of Nuegra was added each time in subjects with no or minimal effect. Testoster-one and FSH levels were measured at first visit and last visit, for evaluating masculine activities. To avoid bias and standardize the test results, only one clinic was assigned as a central lab, and all blood samples were transferred. General information and subjective symptoms were evaluated at first visit and at 2 weeks interval, week 2, 4 and 6 using VAS (Visual Analogue Scale). The mean age of the subjects were 51.8${\pm}$8.2 years old (range: 36.1-82.1). Based on the subjects who were tested on testosterone and FSH levels at day l and week 6, the means were 4.4${\pm}$1.4 nmol/L (range: 2.6-7.7), 8.6${\pm}$9.6 mIU/mL (range: 0.3-40.4), respectively at day 1. At week 6, the results were 4.9 ${\pm}$1.6 (2.6-8.9 range), 9.4${\pm}$13.1 (1.0-53.9 range), respectively. Marginally significant difference between pre-dose and post-dose was present. Statistically significant differences were revealed in general assessment for subjective symptoms, fatigue, insomnia, erectile dysfunction, etc. In fatigue, response rates were 39.6, 65.4 and 76.4% at week 2, 4 and 6, respectively (P < 0.0001). Response vates for erectile dysfunction were 13.4, 41.2 and 72.7% at week 2, 4, and 6 (P < 0.0001), respectively, Response rates for libido were 13.6, 51.6 and 73.5% at week 2, 4, and 6 (P < 0.0001), respectively. For urinary stream response rates were 26.9, 44.7 and 66.8% at week 2, 4, and 6 (P < 0.0001), respectively. VAS for muscular weakness did not show significant results that response rates were 40, 60 and 80% at week 2, 4, and 6 from 8.2 (P = 0.24), respectively. Response rates for insomnia were 50, 60, 100% at week 2, 4, and 6 (P < 0.0001), respectively. The results shows that Nuegra tends to enhance masculine activities including libido, erectile dysfunction and urinary stream and also effective for improving general conditions especially insomnia, muscular weakness and fatigue. In conclusion, this investigation has demonstrated that Nuegra does not only have tendency to increase masculine activities through increased secretion of the testosterone and FSH but also improve general conditions such as erectile dysfunction, libido, fatigue and muscular power.

Distribution of Weed Population in the Winter Wheat and Barley Field in Korea (전국(全國) 맥류재배포장(麥類栽培圃場)의 잡초발생분포(雜草發生分布) 조사(調査))

  • Ha, Y.W.;Nam, Y.I.;Park, M.E.;Cho, C.H.
    • Korean Journal of Weed Science
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    • v.3 no.2
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    • pp.120-128
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    • 1983
  • The nationwide survey of weed population covering 1,800 wheat and barley fields in 60 Cities and Guns all around Korea was conducted in December of 1982 and March-April of 1983. In this survey result, two species of gresses and 35 species of broadleaves were identified, and among them 36 and 29 species were found in the wheat and barley fields of upland and paddy, respectively. In the scope of weed life cycle, biennials were more dominant than perennials and annuals in these fields. The most dominant weed species in upland fields were Stellaria media, Cyrillus and Alopecurus aequalis Sobol var. amurensis Ohwi while those in paddy fields were Stellaria alsine Grimm. var. undulata Ohwi and Alopecurus aequalis Sobol var. amurensis Ohwi. In addition, the more weeds were observed in the conventional row seeding method than in other seeding methods including drill seeding and also in no-tillage than in tillage method. In the other hand, the successive use of herbicide for several years greatly influenced the pattern of weed occurrence; broadleaved weeds increased and grasses decreased with the successive use of herbicides.

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Allelopathic Effect of Sorghum Extract and Residues on Selected Crops and Weeds (수수의 타감작용(他感作用)에 관(關)한 연구(硏究))

  • Kim, S.Y.;De Datta, S.K.;Robles, R.P.;Kim, K.U.;Lee, S.C.;Shin, D.H.
    • Korean Journal of Weed Science
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    • v.14 no.1
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    • pp.34-41
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    • 1994
  • To better understand the allelopathic effect of sorghum(Sorghum vulgare L.), the inhibitory activities of water extracts of the stem, leaf and root, and of residues of the stem to major crops and weeds associated with them were evaluated. The allelopathic activity of sorghum plants was species specific, and depended on source and concentration. Germination, and shoot and root length of all test species were inhibited by the different concentrations of the stem extract. Among the crop species, radish showed the most inhibition, followed by wheat and rice. Maize was the least sensitive species. Of the weed species, Ipomoea triloba was most inhibited, followed by Echinochloa colona and Rottboellia cochinchinensis. The water extracts of leaves, stems, and roots significantly inhibited germination and seedling growth in E. colona and radish. The stem extract gave the greatest inhibitory effect on E. colona while all three extracts produced similar response in radish. In the greenhouse trial, sorghum stem residue placed on the soil surface as mulch significantly inhibited seedling growth in E. colona and radish, but not that in rice.

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