• 생명과학회지
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• 제30권8호
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• pp.708-712
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• 2020

리트로머(retromer)는 VPS26, VPS29, VPS35 분자로 구성된 복합체로, 세포막에 존재하는 특정 단백질을 엔도솜에서 트렌스골지망으로 리사이클에 관여하는 단백질 복합체이다. 2000년대 초반 콜롬비아대학의 Scott A, Small 팀에 의해서, 알츠하이머 환자에서 리트로머 분자의 발현량이 저하된다는 것을 발견하였으며, 리트로머를 구성하는 VPS35 발현을 저하시킨 마우스를 이용한 Morris Water Maze (MWM) 실험에서 인지능력이 떨어진다는 것을 보고 하였다. 본 연구진은 리트로머를 구성하는 VPS26 분자에 대한 서브타입인 VPS26b를 발견하였고, 낙아웃 마우스를 제작하였다. VPS26b 낙아웃 마우스 뇌조직을 이용한 웨스턴 블롯 결과, 낙아웃 마우스 뇌조직에서 VPS29와 VPS35의 발현량의 50% 정도로 감소되는 것을 확인하였다. 또한, VPS29 낙아웃 마우스를 이용하여 MWM실험은 한 결과 인지능력이 저하되는 것을 확인하였으며 뇌조직의 해마 CA3영역의 세포 분포도가 정상마우스에 비해 감소되는 것을 확인하였다. 결과적으로 이번 연구를 통하여 VPS26b 낙아웃 마우스는 뇌질환 연구에 대한 실험 동물로서 기초 자료를 제공할 수 있을 것임을 보여준다.

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1808-1816
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• 2016

As a scaffolding subunit of the PIK3C3/VPS34 complex, Beclin 1 recruits a variety of proteins to class III phosphatidylinositol-3-kinase (VPS34), resulting in the formation of a distinct PIK3C3/VPS34 complex with a specific function. Therefore, the investigation of a number of Beclin 1 domains required for the protein-protein interactions will provide important clues to understand the PIK3C3/VPS34 complex, of which Beclin1-VPS34 interaction is the core unit. In the present study, we have designed a bacterial overexpression system for the Beclin 1 domain corresponding to VPS34 binding (Vps34-BD) and set up the denaturing purification protocol due to the massive aggregation of Vps34-BD in Escherichia coli. The expression and purification conditions determined in this study successfully provided soluble and functional Vps34-BD.

• Reproductive and Developmental Biology
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• 제32권1호
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• pp.65-70
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• 2008

Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

• 전자공학회논문지CI
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• 제38권5호
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• pp.86-92
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• 2001

유전자 알고리즘은 어려운 최적화 문제를 해결하는데 효과적인 알고리즘으로 잘 알려져 있다. 최근 네트워크 디자인에 관련된 문제에 유전자 알고리즘의 응용사례가 증가하고 있다. 본 논문에서는 ATM 망에서 주어진 물리적 네트워크와 트래픽 요구를 바탕으로 최적의 가상경로망을 설계하는데 2단계의 유전자 알고리즘을 제안한다. 첫 번째 단계는 경로 설정 단계로서 모든 네트워크의 노드쌍에 대한 경로를 설정하는 것이고 두 번째 단계는 이를 바탕으로 전체 VPs 수와 링크당 경유하는 VPs 수, VP 이동대상 수를 최소로하기 위한 VPs 설정단계이다. 시뮬레이션을 통하여 제안된 알고리즘의 유용성과 성능을 평가 분석한다.

• 정보보호학회논문지
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• 제23권5호
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• pp.825-830
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• 2013

미국의 Arcot사는 가상 세션을 이용하여 트랜잭션 변경 사항을 표시하는 안전한 온라인 금융 거래 서비스 솔루션을 판매 중이며, 해당 기술의 특허를 출원 중이다. 하지만 VPS(Virtual Private Session)가 제공하는 캡챠의 구성 방식에 의해 취약점을 갖는다. VPS가 제공하는 캡챠는 색상정보를 이용한 공격이 가능함을 보였고, 이는 VPS 또한 안전성을 확신할 수 없음을 시사한다. 이 논문에서는 VPS의 공격 방법을 제시하고 유사 VPS를 만들어 앞서 제시한 방법으로 모의 공격을 통한 취약점을 알아본다.

• Clinics in Shoulder and Elbow
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• 제24권1호
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• pp.32-35
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• 2021

Angioleiomyoma is a benign soft tissue tumor originating from vascular smooth muscle. We report a case of a 20-year-old student who presented with pain in the right shoulder of 4 years duration. Shoulder movements were pain-free throughout the range of motion except resisted external rotation. Magnetic resonance imaging visualized a well-circumscribed lesion over the infraspinatus tendon. The lesion was surgically removed and sent for histopathological analysis. Morphology and immunohistochemistry results were suggestive of angioleiomyoma. The most common location for such a lesion is the lower limb, with less than 1% being reported in the upper arm, of which an angioleiomyoma of the shoulder is extremely rare.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제22권6호
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• pp.581-587
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• 2019

Arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome is a rare autosomal recessive multisystemic disease that is associated with the liver, kidney, skin, and central nervous and musculoskeletal systems. ARC occurs as a result of mutations in the VPS33B (Vacuolar protein sorting 33 homolog B) or VIPAR (VPS33B interacting protein, apical-basolateral polarity regulator) genes. A female infant presented with neonatal cholestasis with a severe clinical outcome. She was diagnosed with ARC syndrome using targeted exome sequencing (TES). Exome sequencing revealed compound heterozygous mutations, c.707A>T and c.239+5G>A, in VPS33B, where c.707A>T was a novel variant; the resultant functional protein defects were predicted via in silico analysis. c.239+5G>A, a pathogenic mutation that affects splicing, is found in less than 0.1% of the general population. Invasive techniques, such as liver biopsies, did not contribute to a differential diagnosis of ARC syndrome; thus, early TES together with clinical presentations constituted an apparently accurate diagnostic procedure.

• Advances in pediatric surgery
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• 제16권2호
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• pp.196-202
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• 2010

Abdominal cerebrospinal fluid pseudocyst is an uncommon complication of ventriculoperitoneal shunt (VPS) performed for hydrocephalus. The incidence of VPS complications in children is higher than in adults. There are controversies and difficulties in the treatment of the abdominal pseudocyst. We report a case of abdominal pseudocyst complicating VPS in a boy. Partial excision of pseudocyst and replacement of the VP shunt were effective during a followup of 18 months postoperatively with no recurrence.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 하계종합학술대회 논문집(3)
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• pp.31-34
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• 2000

Genetic Algorithm is well known as the efficient algorithm which can solve a difficult optimization problem. Recently, there has been increasing interest in applying genetic algorithm to problem related to network design. In this paper, we propose a two-step genetic algorithm for designing a optimum virtual path network(VPN) for a given physical network and traffic demand. The first step is a routing step in which a route is found between every node pair in the network. In the second step, paths are assigned as VPs so as to minimize the total number of VPs configured, the number of VPs carried by a link, and the VP hopcount. We study the performance of the propose algorithm through simulation. The result shows that the VPN generated by the proposed algorithm is good in minimizing the number of VPs configured, the load on a link, and the VP hopcount.

• 한국경영과학회지
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• 제23권4호
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• pp.187-201
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• 1998

In ATM networks with high capacity, the effect of failures on transmission links or nodes can be catastrophic, so that the issue of survivability is of great importance. In this paper. we consider the routing problem for working and backup virtual paths(VPs). To accomplish a higher survivability. routing the two kinds of VPs should be taken into account at the same time because backup VP routing depends on the working VP routing. A coevolutionary algorithm is employed to solve the problem for simultaneously routing of working and backup VPs. To develop an efficient coevolutionary algorithm for the problem. structure of populations, encoding method, neighborhood, and genetic operators are studied in this paper. The results of extensive experiments are reported. The performance comparison of the proposed algorithm with a conventional genetic algorithm and existing heuristics shows that our approach is promising.