Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Molecular Cloning of Vps26a, Vps26b, Vps29, and Vps35 and Expression Analysis of Retromer Complex in Micro Pig

  • Kim, Ek-Yune (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Kim, Young-Hyun (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Ryu, Chung-Hun (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Lee, Jae-Woong (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Kim, Sang-Hyun (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Lee, Sang-Rae (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Kim, Myeong-Su (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Kim, Wan-Jun (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB)) ;
  • Lim, Jeong-Mook (Department of Agricultural Biotechnology, Division of Animal Genetic Engineering, Seoul National University) ;
  • Chang, Kyu-Tae (National Primate Research Center (NPRC), Korea Research Institute of Biotechnology (KRIBB))
  • Published : 2008.03.31
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Abstract

Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

Keywords

References

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