• Journal of Life Science
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• v.30 no.8
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• pp.708-712
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• 2020

Vacuolar protein sorting (VPS) 26b is a newly discovered member of the retromer complex; it is encoded by a single-copy gene located on mouse chromosome 9, and the complex has been reported as being composed of proteins VPS26, VPS29, and VPS35. We have previously shown that mice lacking VPS26b exhibited no significant body size or health issues. Although retromer components are widely expressed in mouse tissue, their roles have not yet been completely elucidated. The current study investigates whether the VPS26b-associated retromer complex can be used as a neurodegeneration model. Previously, we observed a significant reduction in VPS35 and VPS29 in the brain cells of in VPS26b-deficient mice as well as an absence of the VPS26b-VPS29-VPS35 retromer complex despite the normal presence of VPS26a-VPS29-VPS35. Recent studies have suggested that low levels of VPS35 can lead to Alzheimer's disease-like phenotypes including cognitive memory deficits. In this study, we successfully demonstrate an association between the absence of the VPS26b-VPS29-VPS35 retromer complex, reduced cell density in the CA3 region of the hippocampus, and learning disability in VPS26b knock-out mice. The results also indicate that the VPS26b-associated retromer complex affects neurodegenerative disorders and learning processes.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1808-1816
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• 2016

As a scaffolding subunit of the PIK3C3/VPS34 complex, Beclin 1 recruits a variety of proteins to class III phosphatidylinositol-3-kinase (VPS34), resulting in the formation of a distinct PIK3C3/VPS34 complex with a specific function. Therefore, the investigation of a number of Beclin 1 domains required for the protein-protein interactions will provide important clues to understand the PIK3C3/VPS34 complex, of which Beclin1-VPS34 interaction is the core unit. In the present study, we have designed a bacterial overexpression system for the Beclin 1 domain corresponding to VPS34 binding (Vps34-BD) and set up the denaturing purification protocol due to the massive aggregation of Vps34-BD in Escherichia coli. The expression and purification conditions determined in this study successfully provided soluble and functional Vps34-BD.

• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.65-70
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• 2008

Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.38 no.5
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• pp.86-92
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• 2001

The Genetic algorithm is well known as an efficient algorithm which can solve a difficult optimization problems. Recently, there has been increasing interest in applying genetic algorithm to problems related to network design. In this paper, we propose a two step genetic algorithm for designing an optimum virtual path network(VPN) for a given physical network and traffic demand. The first step is to span route between every node pair in the network. The second step assigns VPs to minimize the total number of VPs, the number of VPs carried by a link, and the VPs hopcount. The propose algorithm is evaluated using computer simulation. The result shows that the VPN generated by the proposed algorithm is good in minimizing the number of VPs, the load on a link, and the VPs hopcount.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.23 no.5
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• pp.825-830
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• 2013

CA Arcot corporation in U.S.A has secure on-line financial trade solution and patent that verify whether transaction had change using virtual session. But, VPS(Virtual Private Session) has another vulnerability by way to construct CAPTCHA. We can't fully trust safety of VPS, Cause it could be attacked by using color information of CAPTCHA. In this paper, We suggest the method of attack VPS, and also point out the vulnerability of VPS though simulation.

• Clinics in Shoulder and Elbow
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• v.24 no.1
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• pp.32-35
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• 2021

Angioleiomyoma is a benign soft tissue tumor originating from vascular smooth muscle. We report a case of a 20-year-old student who presented with pain in the right shoulder of 4 years duration. Shoulder movements were pain-free throughout the range of motion except resisted external rotation. Magnetic resonance imaging visualized a well-circumscribed lesion over the infraspinatus tendon. The lesion was surgically removed and sent for histopathological analysis. Morphology and immunohistochemistry results were suggestive of angioleiomyoma. The most common location for such a lesion is the lower limb, with less than 1% being reported in the upper arm, of which an angioleiomyoma of the shoulder is extremely rare.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.22 no.6
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• pp.581-587
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• 2019

Arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome is a rare autosomal recessive multisystemic disease that is associated with the liver, kidney, skin, and central nervous and musculoskeletal systems. ARC occurs as a result of mutations in the VPS33B (Vacuolar protein sorting 33 homolog B) or VIPAR (VPS33B interacting protein, apical-basolateral polarity regulator) genes. A female infant presented with neonatal cholestasis with a severe clinical outcome. She was diagnosed with ARC syndrome using targeted exome sequencing (TES). Exome sequencing revealed compound heterozygous mutations, c.707A>T and c.239+5G>A, in VPS33B, where c.707A>T was a novel variant; the resultant functional protein defects were predicted via in silico analysis. c.239+5G>A, a pathogenic mutation that affects splicing, is found in less than 0.1% of the general population. Invasive techniques, such as liver biopsies, did not contribute to a differential diagnosis of ARC syndrome; thus, early TES together with clinical presentations constituted an apparently accurate diagnostic procedure.

• Advances in pediatric surgery
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• v.16 no.2
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• pp.196-202
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• 2010

Abdominal cerebrospinal fluid pseudocyst is an uncommon complication of ventriculoperitoneal shunt (VPS) performed for hydrocephalus. The incidence of VPS complications in children is higher than in adults. There are controversies and difficulties in the treatment of the abdominal pseudocyst. We report a case of abdominal pseudocyst complicating VPS in a boy. Partial excision of pseudocyst and replacement of the VP shunt were effective during a followup of 18 months postoperatively with no recurrence.

• Proceedings of the IEEK Conference
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• 2000.06c
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• pp.31-34
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• 2000

Genetic Algorithm is well known as the efficient algorithm which can solve a difficult optimization problem. Recently, there has been increasing interest in applying genetic algorithm to problem related to network design. In this paper, we propose a two-step genetic algorithm for designing a optimum virtual path network(VPN) for a given physical network and traffic demand. The first step is a routing step in which a route is found between every node pair in the network. In the second step, paths are assigned as VPs so as to minimize the total number of VPs configured, the number of VPs carried by a link, and the VP hopcount. We study the performance of the propose algorithm through simulation. The result shows that the VPN generated by the proposed algorithm is good in minimizing the number of VPs configured, the load on a link, and the VP hopcount.

• Journal of the Korean Operations Research and Management Science Society
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• v.23 no.4
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• pp.187-201
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• 1998

In ATM networks with high capacity, the effect of failures on transmission links or nodes can be catastrophic, so that the issue of survivability is of great importance. In this paper. we consider the routing problem for working and backup virtual paths(VPs). To accomplish a higher survivability. routing the two kinds of VPs should be taken into account at the same time because backup VP routing depends on the working VP routing. A coevolutionary algorithm is employed to solve the problem for simultaneously routing of working and backup VPs. To develop an efficient coevolutionary algorithm for the problem. structure of populations, encoding method, neighborhood, and genetic operators are studied in this paper. The results of extensive experiments are reported. The performance comparison of the proposed algorithm with a conventional genetic algorithm and existing heuristics shows that our approach is promising.