• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2019년도 추계학술대회
• /
• pp.67-67
• /
• 2019

Vaccinium oldhamii (V. oldhamii) has been reported to exert a variety of the pharmacological properties such as anti-oxidant activity, anti-cancer activity, and inhibitory activity of ${\alpha}$-amylase and acetylcholinesterase. However, the anti-inflammatory activity of V. oldhamii has not been studied. In this study, we aimed to investigate anti-inflammatory activity of the stem extracts from V. oldhamii, and to elucidate the potential mechanisms in LPS-stimulated RAW264.7 cells. Among VOS, VOL and VOF, the inhibitory effect of NO and PGE2 production induced by LPS was highest in VOS treatment. Thus, VOS was selected for the further study. VOS dose-dependently blocked LPS-induced NO and PGE2 production by inhibiting iNOS and COX-2 expression, respectively. VOS inhibited the expression of pro-inflammatory cytokines such as $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$. In addition, VOS suppressed TRAP activity and attenuated the expression of the osteoclast-specific genes such as NFATc1, c-FOS, TRAP, MMP-9, cathepsin K, CA2, OSCAR and ATPv06d2. VOS inhibited LPS-induced $NF-{\kappa}B$ signaling activation through blocking $I{\kappa}B-{\alpha}$ degradation and p65 nuclear accumulation. VOS inhibited MAPK signaling activation by attenuating the phosphorylation of ERK1/2, p38 and JNK. Furthermore, VOS inhibited ATF2 phosphorylation and blocked ATF2 nuclear accumulation. From these findings, VOS has potential to be a candidate for the development of chemopreventive or therapeutic agents for the inflammatory diseases.

• Mycobiology
• /
• 제49권3호
• /
• pp.258-266
• /
• 2021

The velvet regulators VosA and VelB are primarily involved in spore maturation and dormancy. Previous studies found that the VosA-VelB hetero-complex coordinates certain target genes that are related to fungal differentiation and conidial maturation in Aspergillus nidulans. Here, we characterized the VosA/VelB-inhibited developmental gene vidD in A. nidulans. Phenotypic analyses demonstrated that the vidD deleted mutant exhibited defect fungal growth, a reduced number of conidia, and delayed formation of sexual fruiting bodies. The deletion of vidD decreased the amount of conidial trehalose, increased the sensitivity against heat stress, and reduced the conidial viability. Moreover, the absence of vidD resulted in increased production of sterigmatocystin. Together, these results show that VidD is required for proper fungal growth, development, and sterigmatocystin production in A. nidulans.

• Applied Biological Chemistry
• /
• 제41권4호
• /
• pp.213-217
• /
• 1998

An assay for cytochrome P450 monooxygenase activity by determination of the products of organophosphate oxidation via inhibition of acetylcholinesterase was described. Extracts from strains of Oryzaephilus surinamensis selected for resistance to chlorpyrifos-methyl (QVOS 102), fenitrothion (VOS F) and malathion (VOS 3), and a standard susceptible strain VOS 48, were incubated with an organophosphate in the presence of a NADPH-generating system and acetylcholinesterase. The degree of inhibition of the acetylchoinesterase activity was converted to manooxygenase activity using standard curves for the inhibition of acetylcholiesterase by chlorpyrifos-methyl-oxon, fenitrooxon and malaoxan. Activity was detectable in VOS 48 and was present at different increased levels with the different organophosphates in the three resistant strains, suggesting that different forms of P450 might be involved in organophosphate oxidation in these insects. The assays were carried out using crude insect homogenates and much smaller samples of insect material than the standard aldrin to dieldrin assay. It should be possible to use the method for determination of monooxygenase activity in single insert.

• 대한수의학회지
• /
• 제42권1호
• /
• pp.35-43
• /
• 2002

Cryopreservation is commonly used for an efficient utilization of semen, oocytes and embryos but has disadvantage in the survival, development of the post-thawed eggs. The high risk in the survival, development of eggs after thawing is thought to be caused by inappropriate internal regulation of $Ca^{2+}$ and/or formation of intracellular ice crystals. In this experiment, we tested whether the $Ca^{2+}$ current (iCa), a decisive factor to $Ca^{2+}$ entry, was altered in post-thawed oocytes by using whole cell voltage clamp technique. The quality and survival rates of the oocytes derived from both fresh and frozen groups were examined by morphology and FDA-test. Vitrified oocytes (VOs) were incubated for 4 hr after thawing and then donated to this experiment. Ethyleneglycol-ficoll-galactose (EFG) was used as a cryoprotectant for vitrification. The membrane potential was held at -80 mV and step depolarizations of 250 ms were applied from -50 mV to 50 mV in 10 mV increments. The survival rates showed a higher in VOs vitrified with EFG containing $Ca^{2+}$ than in VOs vitrified with EFG under the $Ca^{2+}$-free condition (82.0% vs 14%). In group with/without $Ca^{2+}$, the survival rates were significantly (P<0.01) difference. In the fresh metaphase II oocytes (FOs), current-voltage (I-V) relationship showed that iCa began to activate at -40 mV and reached its maximum at -10 mV. With same voltage pulses, inward currents were elicited in VOs. I-V relationships observed in VOs were similar to those in FOs. Time constants of activation and inactivation of the inward current shown in VOs were not different to those in FOs. This accordance in I-V relations and time constants in FOs with those in VOs indicates that the inward currents in FOs are unaltered by vitrification and thawing. Therefore, vitrification with EFG does not play as a factor to deteriorate $Ca^{2+}$ entry across the membrane of the oocytes.

• 한국통신학회논문지
• /
• 제40권2호
• /
• pp.408-418
• /
• 2015

Virtualizing The Physical Devices And Resources As Well As Conceptual Entities Would Form Vos And Cvos, Which Provides Dynamicity And Intelligence Through Composition And Collaboration For Emergency Services In WoO Based Smart Shopping Mall (WSSM). Semantic Ontology In WoO Platform Supports Dynamic Composition And Collaboration Among Objects, VOs And CVOs To Provide Intelligent Services. This Paper Proposes An Architectural Model Of WoO Platform To Support The Smart Emergency Services In Shopping Mall. A Semantic Ontology Model For Wssm, And Information Reusability And Interoperability Among The Vos And Their Functional Models Have Been Presented.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.227-228
• /
• 2003

The most important of various merits are the capacity to investigate an total genome for polymorphism and AELP is superior to any other systems in terms of the number of sequences amplified per reaction and its reproducibility(Vos et al., 1995). The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of origin o. complexity (Vos et at., 1995). (omitted)

• 정보처리학회논문지D
• /
• 제13D권7호
• /
• pp.1009-1016
• /
• 2006

그리드 컴퓨팅 기반의 모든 응용 작업은 두 개의 요구사항을 필히 만족해야 하는데 그 중 하나는 가상조직의 동적 변화를 수용할 수 있어야 한다는 것이고, 또 다른 하나는 서로 다른 가상조직 사이에 서로 다른 보안 단계와 정책을 적용할 수 있어야 한다는 것이다. 본 논문에서는 우선 위의 요구사항들에 대한 두 개의 가상 시나리오를 통하여 그 중요성을 부각시키는 동시에 그리드 기반의 정보검색 시스템 시제품 개발을 통하여 그 요구사항들이 만족될 수 있음을 보여주었다. 가상조직의 동적 변화특성은 단지 그 조직에 속한 사용자들의 증가와 감소뿐만이 아니라, 전체 시스템을 구성하고 있는 서브시스템들 간에 컴퓨팅 파워에 대한 동적인 변화 요구에도 적용된다. 이는 어느 한 서브시스템으로부터의 컴퓨팅 파워 증가 요청을 시스템 내의 유휴 자원인 다른 서브시스템이 만족시켜 줄 수 있기 때문에 전체적인 시스템 상의 유연성이 유지된다는 장점을 갖는다. 본 논문에서는 글로버스 3.0에서 제공되는 보안기능과 가상조직을 이용하여 그리드 기반 정보검색 시스템을 구현하였고, 동적 가상조직을 위한 본 시스템의 확장성과 보안성이 어떻게 향상될 수 있는지를 보여 주었다. 이와 더불어 본 논문에서는 서로 다른 가상조직들을 관리하기 위한 가상조직 관리서비스를 구현하여 글로버스에 추가 서비스로 등록하였다.

• 인터넷정보학회논문지
• /
• 제11권4호
• /
• pp.73-83
• /
• 2010

그리드 시스템에서의 확장성은 점증하고 있는 그리드 이용자를 수용하기 위해 효율적으로 설계되어야 한다. 본 논문은 그리드환경에서 확장성 가상조직(SVO: Scalable Virtual Organization)을 사용하여 VO들 간의 확장형 자원 공유 기법을 제시한다. 제안하는 그리드 구조는 SVO를 관리하기 위한 그리드 시스템의 표준 서비스들로 구성되며, 통합할 VO들을 선택하기 위해, VO들 간의 유사도를 결정하는 데 사용하는 그룹 유사함수를 제시한다. 다른 유사 함수들을 제시한 기법(GSF)과 비교하고, 시뮬레이션을 통해 SVO의 성능을 평가하였다.

• 한국전기전자재료학회논문지
• /
• 제37권3호
• /
• pp.231-240
• /
• 2024

Titanium dioxide (TiO₂) holds significant scientific and technological relevance as a key photocatalyst and resistive random-access memory, demonstrating unique physicochemical properties and serving as an n-type semiconductor. Understanding the density and arrangement of oxygen vacancies (VOs) is crucial for tailoring TiO₂'s properties to diverse technological needs, driving increased interest in exploring oxygen vacancy complexes and superstructures. In this mini review, we summarize the recent understandings of the fundamental properties of oxygen vacancies in bulk rutile (R-TiO₂) and anatase (A-TiO₂) based on DFT and beyond method. We specifically focus on the excess electrons and their spatial arrangement of disordered single VO in bulk R and A-TiO₂, aligned with the experimental findings. We also highlight the theoretical works on investigating the geometries and stabilities of ordered VOs complexes in bulk TiO₂. This comprehensive review provides insights into the fundamental properties of excess electrons in reduced TiO₂, offering valuable perspectives for future research and technological advancements in TiO₂-based devices.

• 해양환경안전학회지
• /
• 제17권4호
• /
• pp.407-412
• /
• 2011

한국기상청에서는 지난 40여년간 영역기상방송(WE-FAX)을 제공하고 있다. 그러나 북서태평양을 통항하는 대부분의 항해사는 한국 기상청 영역기상방송을 이용하지 않고 일본 및 미국의 영역기상방송을 이용하고 있다. 이에 따라 본 연구에서는 항해사를 대상으로 설문조사와 병행하여 한국, 일본 및 미국의 종관일기도를 조사하여 비교 분석함으로써, 한국기상청의 영역기상방송 서비스 개선 방안을 제시한다. 그 결과, (1) 교육과정중 항해사에게 한국기상청의 영역기상방송 서비스에 대해 홍보하여 인지도를 높인다 (2) 원양선의 VOS 시스템 참여를 유도하여 일기도의 정확도를 제고한다 (3) 외양파랑도 및 지상일기예상도 등 일기도 콘텐츠를 다양화하고 항행안전에 도움이 되는 유익한 기상정보를 추가하는 방안을 제시한다.