• Title/Summary/Keyword: Viviparous

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Improvement of Forage Crop Yield and Retardation of Leaf Senescence by Introduction of Gene for Cytokinin Synthetase into Plants (Cytokinin 합성효소의 도입에 따른 형질전환 식물체의 노화 지연 및 수량의 증가)

  • Lee, B.H.;Won, S.H.;Lee, H.S.;Kim, K.Y.;Kim, M.H.;Eun, S.J.;Jo, J.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.3
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    • pp.281-290
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    • 1999
  • The bacterial isopentenyl transferase (ipt) gene involved in cytokinin biosynthesis was fused with 35S promoter of cauliflower mosaic virus (CaMV) and introduced into tobacco plants (Nicotiana tabacum L. cv. Samsun) via Agrobacterium-mediated transformation. As expected, ipt gene was constitutively expressed in all tissues of transgenic plants. Several primary transgenic plants were obtained that expressed different level of transcripts for ipt gene. Three of transgenic plants with different expression level of ipt gene were selected and selfed to obtain homozygous line for further analysis. A number of interesting phenotypic changes such as viviparous leaves, delayed senescence, larger axillary shoots, an abundance of tiny shoots at the apex and a release of lateral buds, were observed in transgenic plants. Chlorophyll content was 1.5- t.o 4-fold higher in transgenic plants as compared with non-transformed plants. These results indicate that the cytokinin synthesized in transgenic plants could improve forage crop yield by delay of leaf senescence and increase of leaf number.

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Quality Characteristics of Wheat Flours from New Released Iksan370 with Long Spike and Domestic Wheat Cultivars (신육성 다수확 밀 익산370호의 원맥과 밀가루의 품질 특성)

  • Choi, Yong-Seok;Lee, Jae-Kang;Choi, Yong-Hyun;Kim, Young-Hwan;Kang, Chon-Sik;Shin, Malsik
    • Korean journal of food and cookery science
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    • v.31 no.5
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    • pp.551-556
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    • 2015
  • Iksan370 is a long-spike wheat developed by the Rural Development Administration yielding excellent features components such as cold resistance, disease resistance, and viviparous germination. The physicochemical and material properties of the raw wheat and milled flour of Iksan370 were analyzed to derive its appropriate uses. The raw wheat of Iksan370 showed high contents of ash and proteins at 1.71% and 13.7%, respectively. Its test weight of 763.0 g/L was similar to those of other varieties and its 1,000 kernel weight was high at 45.38 g. The milled flour of Iksan370 had an ash content of 0.45%, which corresponds with a class 1 flour, and its protein content is 12.18%, corresponding with strong flour. The damaged starch was 5.41%, which was lower than that of other varieties. The average grain size was $70.67{\mu}m$ and the grain distribution was at the level of a typical hard wheat. In the farinogram, the water absorption was 58.63%, which corresponded to the level of medium flour. The development time was 7.00 minutes, which was significantly lower than those of Jokyung and Keumkang. The degree of softening was 67.00 BU, similar to those of Yunbaek and Baekjoong. Among the physico-chemical characteristics, the high protein content and typical hard wheat grain distribution of Iksan370 were similar to those of strong wheat, usually used for bread making. However, in the farinogram, the dough development time was short and the degree of softening was high. As a result, Iksan370 was expected to have poor breadmaking properties and a small volume of the final bread product due to insufficient dough durability. On the other hand, Iksan370 showed the highest maximum gelatinization viscosity at 864.00 BU. Therefore, Iksan370 is expected to show glutinous texture when used for noodles and its flour appears to be appropriate for frying powders as well.

Disruption of Sex Differentiation by Exogenous Sex Steroid Hormones in Korean Rockfish, Sebastes schlegeli (외인성 성스테로이드 호르몬에 의한 조피볼락, Sebastes schlegeli의 성분화 교란)

  • Kwon, Joon-Yeong;Lee, Chan-Hee;Kim, Ju-Yeong;Kim, Sang-Hun;Kim, Dae-Jung;Han, Hyoung-Kyun;Lim, Han-Kyu;Byun, Sun-Gyu
    • Development and Reproduction
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    • v.10 no.4
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    • pp.247-254
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    • 2006
  • It is well publicized that the existence of various endocrine disrupting chemicals threatens normal sexual development of many sedentary marine fishes in the coastal areas. However, a suitable marine fish species for efficient monitoring of this threatening has yet to be identified. One of the difficulties in estimating the effect of endocrine disruption in marine fish is the absence of clear distinction between testicular and ovarian structures at the early stages of sex differentiation. In search of a potential test species, we have investigated the microscopic structures of sexually undifferentiated and differentiated gonads and the susceptibility of gonadal differentiation to exogenous sex steroids during the sex differentiation period in a sedentary marine rockfish, Sebastes schlegeli. Male gonads in this species contained dark pigmentation that made them distinct from female gonads. Treatment either with $estradiol-17\;{\beta}(E_2)$ or $17\;{\alpha}-methyltestosterone$ (MT) significantly altered the sex ratios with the complete sex changes or the occurrence of ovotestis that was easily identified by the mixed structure of dimorphic gonads (coexistence of ovarian cavity/primary oocytes and dark pigmentation/seminiferous tubules). Results in this study suggest that S. schlegeli can be developed as a monitoring/test fish species for endocrine disruption in marine fish in the coastal areas.

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Deterioration of Agronomic Characteristics of Drought-Resistant GM Rice (CaMsrB2-8) (가뭄저항성 GM벼(CaMsrB2-8)의 농업적 특성의 퇴화 가능성 검정)

  • Kim, Bo-Ra;Son, Jin-Hwan;Kim, Hye-Ryun;Ham, Jung-Kwan;Dhungana, Sanjeev Kumar;Park, Soon-Ki;Shin, Dong-Hyun
    • Weed & Turfgrass Science
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    • v.2 no.2
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    • pp.159-163
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    • 2013
  • The purpose of this study is to observe the growth habit and investigate a possibility of cultivating the GM rice (CaMsrB2-8) as a rice cultivar having drought resistance. Germination viability test showed that there was no significant difference between the drought-resistant GM(CaMsrB2-8) and non-GM (Ilmi) rice which was the parent variety at the GM rice. All the seeds of CaMsrB2-8 and Ilmi germinated after 6 days. Viviparous germination was not found in CaMsrB2-8 and Ilmi that was grown in greenhouse at $23{\pm}2^{\circ}C$ with water spraying for 40 days. Ratooning of CaMsrB2-8 and Ilmi was observed in 7-14 days and found uniform in field condition. CaMsrB2-8 seemed to grow faster than Ilmi. But CaMsrB2-8 and Ilmi were similar in 14-21 days. Both CaMsrB2-8 and Ilmi showed low seed shattering and more than 90% grains were ripened. All the seeds scattered in the paddy soil surface were not germinated after passing the winter. This study suggests that the drought-resistant GM rice was not significantly different with the parent variety of Ilmi in many agronomic characteristics such as wildness traits.

Development of dry milling suitable rice cultivar to invigorate rice processing products

  • Jeung, Ji-Ung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.10-10
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    • 2017
  • Rice consumption has been continuously decreasing as the eating habits of Koreans have become westernized and diversified. The per capita annual rice consumption in Korea has dropped sharply from 136.4 kg in 1970 to 61.9 kg in 2016. The Korean government, therefore, has been trying to promote rice consumption by invigorating the processed food industry using rice flour. To facilitate the market for processed rice foods, it is essential to develop proper milling technology in terms of flour particle size and damaged starch content to produce high quality rice flour at competitive cost. Dry milling and wet milling are the two major processes used to produce rice flour. Although the dry milling process is relatively simple with a lower production cost, damaged starch content increases because of the high grain hardness of rice. In wet milling, the quality of rice flour is improved by reducing flour particle size as well as damaged starch content through soaking procedures. However, the production costs are high because of the additional expenses associated with the disposal of waste water, sterilization and drying of the wet flour. Recently developed technologies such as jet milling and cryogenic milling also require expensive investment and production. Therefore, developing new rice cultivars with dry milling adaptability as well as good processing properties is an important goal of rice breeding in Korea. 'Suweon 542' is a floury endosperm mutant line derived from sodium azide treatment on a high-yield, early maturing, and non-glutinous japonica rice cultivar, 'Namil'. Compared with the wild type, after dry milling process, the grain hardness of 'Suweon 542' was significantly lower because of its round and loosely packed starch granules. Also, the flour of 'Suweon 542' had significantly smaller particles and less damaged starch than 'Namil' and other rice cultivars and its particle size distribution was similar to a commercial wheat cultivar. Recently, through collaborations with nine universities and food companies, a total of 21 kinds of processed prototypes, using the dry milling flour of 'Suweon 542', were evaluated. In the production of major rice processing products, there was no significant quality difference between the flours prepared by wet milling and dry milling. Although the amount of water added to the dough was slightly increased, it was confirmed that the recipe applying the wet flour could be used without significant change. To efficiently transfer the floury endosperm characteristics of 'Suweon 542' to other commercial rice cultivars, it is essential to develop DNA marker tightly linked to the target gene. Association analysis using 70 genome-wide SSR markers and 94 F2 plants derived from 'Suweon 542'/'Milyang 23' showed that markers on chromosome 5 explained a large portion of the variation in floury grains percentage (FGP). Further analysis with an increased number of SSR markers revealed that the floury endosperm of 'Suweon 542' was directed by a major recessive locus, flo7(t), located in the 19.33-19.86 Mbp region of chromosome 5, with RM18639 explaining 92.2% of FGP variation in the F2 population. Through further physical mapping, a co-segregate and co-dominant DNA marker with the locus, flo7(t) was successfully developed, by which, thereby, breeding efficiency of rice cultivars having proper dry milling adaptability with high yield potential or useful functional materials would be improved. 'Suweon 542' maintained the early maturity of the wild type, Namil, which can be used in rice-wheat double cropping systems in Korea not only for improved arable land but also for sharing flour production facilities. In addition to the high susceptibility against major rice diseases, nevertheless, another possible drawback of 'Suweon 542' is the high rate of viviparous under prolonged rainfall during the harvesting season. To overcome susceptibility and vivipary of 'Suweon 542', the progeny lines, derived from the crosses 'Suweon 542' and 'Jopyeong', an early maturing rice cultivar with multiple resistance against rice blast, bacterial blight, and rice strip virus, and 'Heugjinju', a anthocyanin pigment containing black rice cultivar, were intensively evaluated. As the outputs, three dry milling suitable rice elite lines, 'Jeonju614', 'Jeonju615', and 'Jeonju616' were developed.

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A Medium-Maturing and Good Quality Japonica Rice Variety, "Cheongan" (벼 중생 고품질 신품종 "청안")

  • Yang, Sae-Jun;Kim, Yeon-Gyu;Choi, Im-Soo;Cho, Young-Chan;Hwang, Hung-Goo;Hong, Ha-Cheol;Kim, Myeong-Ki;Oh, Myung-Kyu;Shin, Young-Seop;Lee, Jeom-Ho;Choi, Yong-Hwan;Choi, In-Bea;Kang, Kyung-Ho;Yea, Jong-Doo;Lee, Jeong-Heui
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.649-653
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    • 2009
  • "Cheongan" is a new japonica rice variety developed from a cross between SR15225-B-22-1-2-1 and Iksan431 in summer season, 1997 by National Institute of Crop Science, RDA. The line SR15225-B-22-1-2-1 has good canopy architecture and multi-disease and insect resistance, and Iksan431 has translucent milled rice and good eating-quality. Heading date of Cheongan is August 13 in central lowland and mid-mountainous areas. "Cheongan" having culm length of 84 cm shows relatively semi-erect pubescent leaf blade and rigid culm, tolerance to lodging with and good canopy architecture. This variety has 14 tillers per hill and 126 spikelets per panicle. It shows tolerance to heading delay and spikelet sterility comparable to Hwaseongbyeo when exposed to cold stress. Leaf senescence of Cheongan progresses slowly during the ripening stage and the viviparous germination ratio was 59 %, similar to that of Hwaseongbyo. "Cheongan" shows moderately resistance to blast disease, but susceptible to stripe virus and brown planthopper. The milled rice of "Cheongan" exhibits translucent, clear non-glutinous endosperm and medium short grain. It shows similar amylose content of 18.7%, gelatinization temperature, and similar palatability of cooked rice compared to Hwaseongbyeo. The milled rice yield of this cultivar is about 5.54 MT/ha at ordinary season culture in local adaptability test for three years. Especially, "Cheongan" has better milling properties of higher 98.4% and 73.9% in the percentage of head rice in milled rice and milling recovery of head rice, respectively, than those of Hwaseongbyeo. "Cheongan" could be adaptable to the central and mid-southern plain area, and mid-western coastal area of Korea.

Bacterial Blight Resistant Mid-late Maturing Rice 'Manbaek' with High Grain Quality (벼흰잎마름병 저항성 고품질 중만생 벼 '만백')

  • Park, Hyun-Su;Baek, Man-Kee;Kim, Bo-Kyeong;Kim, Ki-Young;Shin, Woon-Chul;Ko, Jae-Kwon;Nam, Jeong-Kwon;Kim, Woo-Jae;Cho, Young-Chan;Ko, Jong-Cheol;Kim, Jeong-Ju;Kim, Hyun-Soon
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.235-244
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    • 2017
  • 'Manbaek' is a bacterial blight resistant mid-late maturing rice cultivar with high grain quality. 'Manbeak' was derived from anther culture using the backcross combination, $Hopum^*2/SR30075$. 'Hopum' is a mid-late maturing rice cultivar with high grain quality and 'SR30075' is a pyramid line carrying three bacterial blight resistance genes. 'Manbaek' was selected through the pedigree method, yield trials, and local adaptability tests. 'Manbeak' carrying two bacterial blight resistance gene Xa3 and xa5 showed high-level and broad-spectrum resistance against bacterial blight. 'Manbaek' was resistant to K3a, mostly virulent race in Korea, and exhibited resistance reaction against 16 Korean bacterial blight isolates. 'Manbaek' was a mid-late maturing rice. The heading date of 'Manbaek' was August 19th, which was 5 days later than that of 'Nampyeong'. Manbaek' was a lodging-tolerant rice with short culm and dark green leaf. Due to the low viviparous germination, 'Manbaek' could be a useful material to prevent pre-harvest sprouting. 'Mabeak' was resistant to bacterial blight and rice stripe virus, but susceptible to other virus diseases and insect pests. The yield of 'Manbaek' was similar to 'Nampyeong'. 'Manbaek' showed excellent grain appearance and good tastes of cooked rice, so that it could contribute to improving the quality of bacterial blight resistant cultivars. 'Manbaek', bacterial blight resistant cultivar with high grain quality, is suitable for the cultivation at bacterial blight prone area and has been utilized in the breeding programs for enhancing the resistance against bacterial blight (Registration No. 6069).

A Very Early-Maturing, Cold Tolerant and High Quality japonica Rice Variety 'Hanseol' (극조숙 고품질 내냉성 벼 신품종 '한설')

  • Lee, Jeong-Heui;Shin, Young-Seop;Jeong, O-Young;Kim, Myeong-Ki;Kim, Yeon-Gyu;Kim, Hong-Yeol;Lee, Jeom-Ho;Lee, Jeong-Il;Cho, Young-Chan;Jeon, Yong-Hee;Choi, Yong-Hwan;Yang, Chang-Ihn;Hong, Ha-Cheol;Won, Yong-Jae;Shin, Jin-Chul;Kim, Hyung-Yoon;Seo, Dae-Ha;Hwang, Hung-Goo;Yea, Jong-Doo
    • Korean Journal of Breeding Science
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    • v.42 no.6
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    • pp.632-637
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    • 2010
  • 'Hanseol' is a new very early-maturing, cold tolerant and high quality japonica rice variety developed from a cross of 'Jinbu24' and 'Jinbu25' by the rice breeding team of National Institute of Crop Science (NICS), Rural Development Administration (RDA) in 2009. The heading date of this variety is July 25, which is four days earlier than check variety, 'Jinbubyeo'. 'Hanseol' has 65 cm of culm length, 99 spikelets per panicle, 82.9% of ripened grain rate, and 21.5 g of 1,000 grain-weight of brown rice. This variety shows susceptibility to bacterial leaf blight and virus diseases, and insect pests. It is tolerant to cold stress in terms of less heading delay and high fertility in cold water irrigated cultivation. This variety shows delayed leaf senescence and considerable tolerance to viviparous germination at ripening stage. The milled rice of this variety exhibits translucent, clear non-glutinous endosperm and medium-short grain. 'Hanseol' showed low gelatinization temperature and 6.1% protein content, 19.1% amylose content and good palatability of cooked rice. The milled rice yield of this variety is about 5.43 MT/ha at ordinary culture in local adaptability test for three years. 'Hanseol' would be highly adaptable to mid-north and mid-mountainous areas, and mid-northern alpine area in Korea.

A Medium-Maturing, High Non-Dietary Starch, Specialty Rice Cultivar 'Goami 3' (벼 중생 고 식이섬유 특수미 신품종 '고아미3호')

  • Lee, Sang-Bok;Lee, Jeom-Ho;Shin, Young-Seop;Lee, Kyu-Seong;Hwang, Hung-Goo;Jeong, O-Young;Yang, Chang-Ihn;Choi, Yong-Hwan;Yang, Sae-Jun;Jeon, Yong-Hee;Hong, Ha-Cheol;Kim, Hong-Yeol;Cho, Young-Chan;Lee, Jeong-Heui;Yea, Jong-Doo;Oh, Myung-Kyu;Kim, Myeong-Ki;Kim, Yeon-Gyu;Jeong, Kuk-Hyun;Lee, Young-Tae
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.595-599
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    • 2011
  • 'Goami 3' is a new japonica rice cultivar developed from a cross between Suweon464 and Daeanbyeo by the rice breeding team of National Institute of Crop Science, RDA. 'Goami3' has about 130 days growth duration from transplanting to heading in central plain area of Korea. It has a good semi-erect plant type and resistance to lodging of about 79 cm in culm length. 'Goami 3' had 15 panicles per hill and 104 spikelets per panicle. 'Goami 3' has very high amylose (29.5%) and high non-dietary starch compared with Hwaseongbyeo. This rice variety has slow senescence and 47% tolerance to viviparous germination during the ripening stage. 'Goami 3' is susceptible to leaf blast, bacterial blight, virus disease and insect pest. The yield performance of this cultivar in milled rice was about 3.92MT/ha by ordinary season culture in local adaptability test from 2005 to 2007. 'Goami 3' is adaptable to central and southern plain area of Korea.

A Lodging Tolerant, Opaque Rice Cultivar 'Seolgaeng' (벼 내도복 뽀얀멥쌀 신품종 '설갱')

  • Hong, Ha-Cheol;Moon, Huhn-Pal;Choi, Hae-Chune;Hwang, Hung-Goo;Kim, Yeon-Gyu;Kim, Hong-Yeol;Yea, Jong-Doo;Shin, Young-Seop;Kang, Kyung-Ho;Choi, Yong-Hwan;Cho, Young-Chan;Baek, Man-Kee;Yang, Chang-Ihn;Choi, Im-Soo;Ahn, Sang-Nag;Yang, Sae-June
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.532-537
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    • 2011
  • 'Seolgaeng' is a new Japonica rice variety developed from an 'Ilpumbyeo' mutant line, N-methyl-N-nitrosourea (MNU) treatment on fertilized egg cell, by a rice breeding team of National Crop Experiment Station, Rural Development Administration in 2001. This variety has about 134 days of growth duration from transplanting to harvesting in central plain area of Korea, a good semi-elect plant type and resistant to lodging with strong culm. The number of panicles/hill of 'Seolgaeng' is more than that of 'Hwaseongbyeo'. The milled rice appearance of this cultivar is an opaque, but it has amylose content (19.3%) similar to 'Hwaseongbyeo'. It is superior to hypae formation of Aspergillus orzyae in the making of fermented rice and amount of pigmentation in fermented rice by Monascus anka that of 'Ilpumbyeo'. This variety showed slow leaf senescence and considerable resistance to viviparous germination. It is moderately resistant to leaf blast and susceptible to bacterial blight, virus disease and insect pests. The yield performance of this rice cultivar is about 5.27 MT/ha in milled rice in local adaptability test for three years from 1999 to 2001). 'Seolgaeng' is adaptable to central and southern plain areas of Korea.