• 생약학회지
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• 제40권3호
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• pp.165-172
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• 2009

Korean folk medicine 'MeoRuIp' has been used to cure cough, rheumatism and abdominal pain after child birth. There has been no pharmacognostical confirmation on the botanical origin of the crude drug. To clarify the botanical origin of 'MeoRuIp', the morphological and anatomical characteristics of the leaves of Vitis and Ampelopsis species growing in Korea, i.e. Vitis amurensis, V. amurensis forma. glabrescens, V. flexuosa, V. thunbergii var. sinuata and Ampelopsis brevipedunculata var. heterophylla were studied. As a result, it was clarified that 'MeoRuIp' was the leaf of Vitis amurensis and Vitis flexuosa.

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.125-134
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• 2017

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

• The Plant Pathology Journal
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• 제32권4호
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• pp.347-356
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• 2016

Plants protect themselves from pathogen attacks via several mechanisms, including hypersensitive cell death. Recognition of pathogen attack by the plant resistance gene triggers expression of carboxylesterase genes associated with hypersensitive response. We identified six transcripts of carboxylesterase genes, Vitis flexuosa carboxylesterase 5585 (VfCXE5585), Vf-CXE12827, VfCXE13132, VfCXE17159, VfCXE18231, and VfCXE47674, which showed different expression patterns upon transcriptome analysis of V. flexuosa inoculated with Elsinoe ampelina. The lengths of genes ranged from 1,098 to 1,629 bp, and their encoded proteins consisted of 309 to 335 amino acids. The predicted amino acid sequences showed hydrolase like domains in all six transcripts and contained two conserved motifs, GXSXG of serine hydrolase characteristics and HGGGF related to the carboxylesterase family. The deduced amino acid sequence also contained a potential catalytic triad consisted of serine, aspartic acid and histidine. Of the six transcripts, Vf-CXE12827 showed upregulated expression against E. ampelina at all time points. Three genes (VfCXE5585, VfCXE12827, and VfCXE13132) showed upregulation, while others (VfCXE17159, VfCXE18231, and VfCXE47674) were down regulated in grapevines infected with Botrytis cinerea. All transcripts showed upregulated expression against Rhizobium vitis at early and later time points except VfCXE12827, and were downregulated for up to 48 hours post inoculation (hpi) after upregulation at 1 hpi in response to R. vitis infection. All tested genes showed high and differential expression in response to pathogens, indicating that they all may play a role in defense pathways during pathogen infection in grapevines.

• 한국약용작물학회지
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• 제15권5호
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• pp.319-323
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• 2007

Wild grapes have been used as traditional medicinal use and alcoholic beverage production in Korea. The objective of this study is to improve antioxidant properties in Sae-muru by far infrared ray (FIR) treatment, with expecting potential benefits of FIR treatment for wild grape products during manufacturing processes. FIR treatment in berries induced increased content of catechin, epicatechin gallate, epigallocatechin gallate, gallic acid, rutin, ellagic acid, and resveratrol, while content of epicatechin and epigallocatechin was decreased. Although FIR treatment resulted either increased or decreased chemical component groups, presenting in HPLC chromatograms, antioxidant activity in Sae-muru extract was significantly increased by the FIR treatment. Our results suggest that FIR treatment should be an efficient process in the production of high content of bioactive molecules in Sae-muru.

• 한국자원식물학회지
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• 제18권2호
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• pp.285-301
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• 2005

본 조사는 식물상을 밝힘으로서 체계적이고 효율적인 보존관리대책을 마련하데 필요한 정보를 제공하고 우리나라 최남단에 속하는 도서에 있어서 식물자원의 분포특성을 파악하고자 수행하였다. 관속식물은 65과 140속 162종 2변종으로 총 164분류군이었는데 그 중 양치식물은 6과 13속 15종, 나자식물은 1과 1속 1종, 피자식물에 있어서는 쌍자엽식물이 49과 97속 114종 1변종으로 115분류군, 단자엽식물은 10과 29속 32종 1변종으로 33분류군이 출현하여 계 59과 126속 146종 2변종으로 계 148종이었다. 미기록 종은 양치식물 중 점고사리, 쇠고비, 큰족제비고사리, 사다리고사리 등 4종, 단자엽식물 중 강아지풀, 줄, 송이고랭이, 꽃토란, 개구리밥, 부채마 등 6종, 쌍자엽식물 중 큰사방오리, 개모시풀, 애기싱아, 미국자리공, 갯장구채, 새머루, 누리장나무, 보리장나무. 진득찰 등 9종 총 16과 19속 19종이었다. 희귀식물로서는 국내 거문도와 제주도에만 분포하는 것으로 알려진 박달목서가 자생하고 있으며, 바위연꽃의 자생지도 확인되었다. 또한 귀화식물은 총 20과 33속 34종 1변종으로 총 35분류군이었다.

• 한국환경생태학회지
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• 제24권1호
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• pp.14-25
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• 2010

본 연구는 염해지역의 식재수종 선발에 대한 기초자료로 활용하고자 한국 남해안 방풍림 자생수목의 토양염분에 대한 내성과 염분비산 영향을 미치는 지대별 토양염분도와 출현수종을 조사 분석하였다. 조사지의 토양염분 $EC_{1:5}$는 전체 평균 $0.18dSm^-1$이었고, 최저 $0.05dSm^{-1}$, 최고 $0.58dSm^{-1}$이었다. 토양염분($EC_{1:5}$)은 I 지대를 제외하고는 해안 정선으로 부터 내륙으로 갈수록 낮았으며, II 지대>llI지대>I 지대>IV지대 순으로 각각 $EC_{1:5}$ $0.22dSm^{-1}$ $0.22dSm^{-1}$ $0.19dSm^{-1}$ $0.13dSm^{-1}$이었다. 출현한 자생식물은 45과 74속 9변종 100종 총 110분류군이었다. 토양염분이 가장 높은 단계인 $EC_{1:5}$ $0.50dSm^{-1}$를 초과하는 곳에 출현한 식물은 담쟁이덩굴과 안동이였으며, 그 다음으로 $EC_{1:5}$ $0.41{\sim}dSm^{-1}$까지 자생하는 식물은 꾸지뽕나무, 멍석딸기, 산초나무, 송악, 아까시나무, 졸참나무, 좀작살나무 퉁이었다. 전제 지대에 출현하는 수종은 정과 새머루이었고, 내조성이 높은 순비기나무는 I 지대에만 출현하였다. 전체 지대에서 중요도가 가장 높온 수종은 느티나무, 팽나무, 모감주나무, 예덕나무, 마삭줄, 칡 등이었다. 이러한 수종은 조사지의 자생 수종들 중에서 다른 수종에 버하여 상대적으로 내조성이 강한 수종으로 판단되었다.