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http://dx.doi.org/10.5010/JPB.2017.44.2.125

Three transcripts of EDS1-like genes respond differently to Vitis flexuosa infection  

Islam, Md. Zaherul (On-Farm Research Division, Bangladesh Agricultural Research Institute)
Yun, Hae Keun (Department of Horticulture and Life Science, Yeungnam University)
Publication Information
Journal of Plant Biotechnology / v.44, no.2, 2017 , pp. 125-134 More about this Journal
Abstract
Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.
Keywords
Disease resistance; Elsinoe ampelina; Enhanced disease susceptibility1; Hypersensitive response; Rhizobium vitis;
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1 Osterlund T, Danielsson B, Degerman E, Contreras JE, Edgren G, Davis RC, Schotz MC, Holm C (1996) Domain structure analysis of recombinant hormone sensitive lipase. Biochem J 319:411-420   DOI
2 Pan Q, Wendel J, Fluhr R (2000) Divergent evolution of plant NBS-LRR resistance gene homologues in dicot and cereal genomes. J Mol Evol 50:203-213   DOI
3 Parker J, Holub E, Frost L, Falk A, Gunn N, Daniels M (1996) Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes. Plant Cell 8:2033-2046   DOI
4 Aarts N, Metz M, Holub E, Staskawicz B, Daniels M, Parker J (1998) Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis. Proc Natl Acad Sci USA 95: 10306-10311   DOI
5 Ahn S, Kim S, Jo S, Yun H (2014) De novo transcriptome analysis of Vitis flexuosa grapevine inoculated with Elsinoe ampelina. Plant Genet Resour-Charact Util 12:S130-S133   DOI
6 Ahn S, Kim S, Kim S, Yun H (2012) Differential expression screening of defense related genes in Vitis flexuosa grapevine against Elsinoe ampelina and Rhizobium vitis. Korean J Intl Agri 24:470-476
7 Singh I, Shah K (2012) In silico study of interaction between rice proteins enhanced disease susceptibility 1 and phytoalexin deficient 4, the regulators of salicylic acid signalling pathway. J Biosci 37:563-571   DOI
8 Reina J, Guerrero C, Heredia A (2007) Isolation, characterization, and localization of AgaSGNH cDNA: a new SGNH-motif plant hydrolase specific to Agave americana L. leaf epidermis. J Exp Bot 58:2717-2731   DOI
9 Rogers E, Ausbel F (1997) Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression. Plant Cell 9:305-316   DOI
10 Rusterucci C, Aviv D, Holt B, Dangl J, Parker J (2001) The disease resistance signaling components EDS1 and PAD4 are essential regulators of the cell death pathway controlled by LSD1 in Arabidopsis. Plant Cell 13:2211-2224   DOI
11 Oirdi M, Bouarab K (2007) Plant signalling components EDS1 and SGT1 enhance disease caused by the necrotrophic pathogen Botrytis cinerea. New Phytol 175:131-139   DOI
12 Staskawicz B, Ausubel F, Baker B, Ellis J, Jones J (1995) Molecular genetics of plant disease resistance. Science 268:661-667   DOI
13 Dangl J, Jones J (2001) Plant pathogens and integrated defense responses to infection. Nature 411:826-833   DOI
14 Baudouin E, Charpenteau M, Roby D, Macro Y, Ranjeva R, Ranty B (1997) Functional expression of a tobacco gene related to the serine hydrolase family esterase activity towards shortchain dinitrophenyl axcylesters. Eur. J. Biochem 248:700-706   DOI
15 Bezier A, Lamber B, Baillieul F (2002) Cloning of a grapevine Botrytis-responsive gene that has homology to the tobacco hypersensitivity-related hsr203J. J Exp Bot 53:2279-2280   DOI
16 Taler D, Galperin M, Benjamin I, Cohen Y, Kenigsbuch D (2004) Plant R genes that encode photorespiratory enzyme confer resistance against disease. Plant Cell 16:172-184   DOI
17 Wagner S, Stuttmann J, Rietz S, Guerois R, Brunstein E, Bautor J, Niefind K, Parker JE (2013) Structural basis for signaling by exclusive EDS1 Heteromeric complexes with SAG101 or PAD4 in plant innate Immunity. Cell Host Microbe 14: 619-630   DOI
18 Century K, Holub E, Staskawicz B (1995) NDR1, a locus of Arabidopsis thaliana that is required for disease resistance to both a bacterial and fungal pathogen. Proc Natl Acad Sci USA 92:6597-6601   DOI
19 Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol 11: 113-116   DOI
20 Chong J, Le Henanff G, Bertsch C, Walter B (2007) Identification, expression analysis and characterization of defense and signaling genes in Vitis vinifera. Plant Physiol Biochem 46: 469-481
21 Delaney T, Friedrich L, Ryals J (1995) Arabidopsis signal transduction mutant defective in chemically and biologically induced disease resistance. Proc Natle Acad Sci USA 92: 6602-6606   DOI
22 Eastwell K, Sholberg P, Sayler R (2006) Characterizing potential bacterial biocontrol agents for suppression of Rhizobium vitis, causal agent of crown gall disease in grapevines. Crop Prot 25: 1191-1200   DOI
23 Eibach R, Diehl H, Alleweldt G (1989) Untersuchungen zur vererbung von resistenzeigenschaften bei reben gegen Oidium tuckeri, Plasmopara viticola and Botrytis cinerea. Vitis 28: 209-228
24 Xiao S, Calis O, Patrick E, Zhang G, Charoenwattana P, Muskett P, Parker JE, Turner JG (2005) The atypical resistance gene, RPW8, recruits components of basal defence for powdery mildew resistance in Arabidopsis. Plant J 42:95-110   DOI
25 Wang J, Shine M, Gao Q, Navarre D, Jiang W, Liu C, Chen Q, Hu G, Kachroo A (2014) Enhanced disease susceptibility1 mediates pathogen resistance and virulence function of a bacterial effector in soybean. Plant Physiol 165:1269-1284   DOI
26 Wang Q, Zhang Y, Gao M, Jiao C, Wang X (2011) Identification and expression analysis of a pathogen responsive PR-1 gene from Chinese wild Vitis quinquangularis. Afr J Biotechnol 10:17062-17069
27 Wiermer M, Feys B, Parker J (2005) Plant immunity: the EDS1 regulatory node. Curr Opin Plant Biol 8:383-389   DOI
28 Yun H, Park K, Roh J, Choi Y, Jeong S (2007) Developing a screening system for resistance to anthracnose in grapevines using culture filtrates from Elsinoe ampelina. J Hortic Sci Biotech 82:360-364   DOI
29 Zhou N, Tootle T, Tsui F, Klessig D, Glazebrook J (1998) PAD4 functions upstream from salicylic acid to control defense responses in Arabidopsis. Plant Cell 10:1021-1030   DOI
30 Falk A, Feys B, Frost L, Jones J, Daniels M Parker J (1999) EDS1, an essential component of R gene-mediated diseases resistance in Arabidopsis has homology to eukaryotic lipases. Proc Natl Acad Sci USA 96:3292-3297   DOI
31 Gao F, Shu X, Ali M, Howard S, Li N, Winterhagen P, Qiu W, Gassmann W (2010) A functional EDS1 ortholog is differentially regulated in powdery mildew resistant and susceptible grapevines and complements an Arabidopsis eds1 mutant. Planta 231:1037-1047   DOI
32 Cao H, Bowling S, Gordon S, Dong X (1994) Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance. Plant Cell 6:1583-1592   DOI
33 Glazebrook J (2001) Genes controlling expression of defense responses in Arabidopsis. Curr Opin Plant Biol 4:301-308   DOI
34 Garcia A, Blanvillain-Baufume S, Huibers R, Wiermer M, Li G, Gobbato E, Rietz S, Parker J (2010) Balance nuclear and cytoplasmic activities of EDS1 are required for a complete plant innate immune response. PLos Pathog 6:e1000970   DOI
35 Gasteiger E, Gattiker A, Hoogland C, Ivanyi I, Appel R, Bairoch A (2003) ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res 31:3784-3788   DOI
36 Geourjon C, Deleage G (1995) SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments. Appl Biosci 11:681-684
37 Glazebrook J, Zook M, Mert F, Kagan I, Rogers E, Crute I, Holub E, Hammerschmidt R, Ausbel F (1997) Phytolexin-deficient mutants of Arabidopsis reveal that PAD4 encodes a regulatory factor and that four PAD genes contribute to downy mildew resistance. Genetics 146:381-392
38 Heidrich K, Wirthmueller L, Tasset C, Pouzet C, Deslandes L, Parker J (2011) Arabidopsis EDS1 connects pathogen effector recognition to cell compartment-specific immune response. Science 334:1401-1404   DOI
39 Guruprasad K, Reddy B, Pandit M (1990) Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence. Protein Eng 4:155-161   DOI
40 Hammond-Kosack K, Jones J (1996) Resistance gene-dependent plant defense responses. Plant Cell 8:1773-1791   DOI
41 Jirage D, Tootle T, Reubert T, Frost L, Feyes B, Parker J, Ausubel F, Glazebrook J (1999) Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling. Proc Natl Acad Sci USA 96:13583-13588   DOI
42 Kortekamp A, Welter L, Vogt S, Knoll A, Schwander F, Topfer R, Zyprian E (2008) Identification, isolation and characterization of a CC-NBS-LRR candidate disease resistance gene family in grapevine. Mol Breeding 22:421-432   DOI
43 Joshi R, Nayak S (2011) Functional characterization and signal transduction ability of nucleotide-binding site-leucine-rich repeat resistance genes in plants. Genet Mol Res 10:2637-2652   DOI
44 Kong X, Lv W, Jiang S, Zhang D, Cai G, Pan J, Li D (2013) Genome-wide identification and expression analysis of calcium-dependent protein kinase in maize. BMC Genomics 14:433   DOI
45 Kono A, Nakaune R, Yamada M, Nakano M, Mitani N, Ueno T (2009) Effect of culture conditions on conidia formation by Elsinoe ampelina, the causal organism of grapevine anthracnose. Plant Dis 93:481-484   DOI
46 Meyers B, Dickerman A, Michelmore R, Sivaramakrishnan S, Sobral B, Young N (1999) Plant disease resistance genes encode members of an ancient and diverse protein family with in the nucleotide-binding superfamily. Plant J 20:317-332   DOI
47 Lee G, Chepyshko H, Chen H, Chu C, Chou Y, Akoh CC, Shaw J (2010) Genes and biochemical characterization of the three novel chlorophyllase isozymes from Brassica oleracea. J Agr Food Chem 58:8651-8657   DOI
48 Letunic I, Doerks T, Bork P (2009) SMART 6: Recent updates and new developments. Nucleic Acids Res 37: D229-D232 (database issue)   DOI
49 Liu J, Liu X, Dai L, Wang G (2007) Recent progress in elucidating the structure, function and evolution of disease resistance genes in plants. J Genet Genomics 34:765-776   DOI
50 Nawrath C, Metraux J (1999) Salicylic acid induction deficient mutants of Arabidopsis expess PR-2 and PR-5 and accumulate high levels of camalexin after pathogen inoculation. Plant Cell 11:1393-1404