• Title/Summary/Keyword: Vitis

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Effect of Light on Production of Athocyanin and Betacyanin Thruough Cell Suspension Culture Systems in Vitis vinyfera L. and Phytolacca americana L. (포도와 미국자리공 세포현탁배양계 안토시아닌과 베타시아닌에 미치는 광의 영향)

  • 최관삼;인준교;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.47-53
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    • 1994
  • The effects of light on the production of anthocyanin and betacyanin in cell suspension cultures of Vitis vinifera and Phytolacca americana were investigated. The cell growth of V.vinifera was little affected by exposure to light, but that of P.americana was markedly increased by light than in the dark In suspension cultures of V vinifera maximum accumulation of anthocyanin was observed during the stationary phase in continuous light By contrast, in suspension cultures of R americana, accumulation of betacyanin occured in parallel with cell division which showed two peaks after 4 days and 8 days of culture in continuous light whereas in continuous dark accumulation of anthocyanin and betacyanin did not occured However treatment of light interrupting for l, 12, and 24 h after 4 days in cell suspension. cultures of remarkably showed a slight anthocyanin accumulation, but after 8 days of culture remarkably accumulated by light interrupting for more than 12 h. In cultures of P. americana, the light treatment was more effective at 4th day than at 7th day after culture, but betacyanin accumulation was decreased again in the dark after light treatment These result indicate that the difference of light responses exist between the V.vinifera and the betacyanin of P. americana though cell suspension culture systems.

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Rhizopus Soft Rot on Grape Caused by Rhizopus stolonifer in Korea (Rhizopus stolonifer에 의한 포도 무름병)

  • Kwon, Jin-Hyeuk;Shim, Chang-Ki;Chae, Yun-Seok;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.57-60
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    • 2007
  • A rhizopus soft rot caused by Rhizopus stolonifer occurred sporadically on grape in orchard in Gyeongsang-nam-do Agricultural Research and Extension Services and in Wholesale Market of Agricultural Products in Jinju city. The infected fruits were rapidly water-soaked, softened and eventually rotted, and the symptoms were initiated from wounds or cracks occurred at harvest time. Sporangiophores were $850{\sim}1,800{\mu}m$ in length and $12{\sim}23{\mu}m$ in width. Sporangia were globose or hemispheric and $80{\sim}190{\mu}m$ in size. The color of sporangia was white and cottony at first and gradually to brownish black, bearing abundant spores when it matured. Columella were hemispheric and $70{\sim}95{\mu}m$ in size. Sporangiospores were irregular round or oval, brownish-black streaked and $8{\sim}22{\times}6{\sim}10{\mu}m$ in size. The optimum temperature for mycelial growth of the fungus on PDA was 25$^{\circ}C$. On the basis of mycological characteristics and pathogenicity to grape (Vitis vinifera), the causal fungus was identified as Rhizopus stolonifer (Ehrenberg ex. Fr,) Lind. This is the first report of Rhizopus soft rot by R. stolonifer on grape (V. vinifera) in Korea.

Selection of Resistant Rootstock and Development of Overwintering Methods for Control of Crown Gall Disease on Grapevine (거봉의 뿌리혹병 방제를 위한 저항성 대목 선발 및 월동법)

  • Kang, Sung-Su;Park, Sang-Heon;Park, Mun-Kyun;Park, Tae-Jin;Kang, Hee-Wan;Choi, Jae-Eul
    • Research in Plant Disease
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    • v.13 no.2
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    • pp.98-103
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    • 2007
  • Grapevines will experience various types of winter damage. Some winter damages are caused by mechanical injury, freezing temperatures or poor vine vigor. This research was conducted to find out the appropriate control methods through selection of resistant rootstocks and improvement of overwintering methods for the control of crown gall disease on 'Kyoho' grape. The crown gall symptoms were not found when three stock plants of grapevine SO4, 5BB and 3306 were inoculated with $10^4cfu/ml$ of Agrobacterium vitis strains (YK2823, YK3312, LMG259, HKA234). But when they were inoculated with higher concentration $(10^6 cfu/ml)$ of A. vitis, irrespective of stocks plants, crown galls were formed all of them and the gall size was much smaller than that of kyoho. Three stock plants were selected as resistant based on above mentioned. Covering trunks and branches with rice straw and insulating coverlet was the most effective method for prevention of crown gall disease. This treatment minimized the ambient temperature changes on grapevine trees during winter season to $9.6^{\circ}C$ and the normal plant growth was due to the absence of freezing injury.

Fruit Characteristics Based on Leaf to Fruit Ratio in 'Pione' Grapevine (Vitis vinifera × V. labrusca) during Cultivation with Heating (가온 재배 시 '피오네' 포도(Vitis vinifera × V. labrusca)의 엽과비에 따른 과실 특성)

  • Yun, Seok Kyu;Park, Seo Jun;Jung, Sung Min;Kim, Jung Bae;Yoon, Ik Koo;Nam, Eun Young;Yu, Duk Jun;Lee, Hee Jae
    • Korean Journal of Environmental Agriculture
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    • v.33 no.1
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    • pp.57-62
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    • 2014
  • BACKGROUND: Defoliation in grapevine cultivation is practically used to improve light environment within the canopy and thereby fruit quality. Effects of defoliation in five-year-old 'Pione' grapevine during cultivation with heating were investigated to find out optimum ratio of leaf area to fruit cluster weight (L/F). METHODS AND RESULTS: The grapevines were defoliated with berry-thinning 20 days after full bloom to provide various levels of L/F. At harvest, total leaf area values of fruit bearing branches were between 0.23 and $0.60m^2$. With increasing L/F, soluble solids and anthocyanin contents curvilinearly increased ($R^2=0.76^{**}$). At L/F over $0.6m^2/kg$, soluble solids content (SSC) leveled off. With increasing L/F, titratable acidity (TA) linearly decreased ($R^2=0.87^{**}$), but the ratio of SSC to TA linearly increased ($R^2=0.86^{**}$). Anthocyanin content was significantly correlated with SSC and the ratio of SSC to TA ($R^2=0.80^{**}$ and $0.82^{**}$, respectively). When total leaf area per fruit bearing branch was maintained $0.40m^2$, soluble solids and anthocyanin contents linearly decreased ($R^2=0.79^{**}$ and $0.85^{**}$, respectively), but TA linearly increased with increasing fruit cluster weight ($R^2=0.70^{**}$). Fruit was low in quality when the L/F was below $0.6m^2/kg$. CONCLUSION: L/F is recommended to be maintained at least $0.6m^2/kg$ in 'Pione' grapevine during cultivation with heating to produce higher-quality fruits.

The maximum limiting characteristic method-based land suitability assessment for peaches (Prunus persica) and grapes (Vitis vinifera L.) using rasterized data of soil and climate on agricultural land in South Korea (토양 및 기후정보 통합 최대저해인자법에 의한 복숭아와 포도의 적지 평가)

  • Kim, Hojung;Koo, Kyung-Ah;Shim, Kyo-Moon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.4
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    • pp.286-296
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    • 2019
  • Land suitability assessments have been a crucial issue for enhancing productivity in agriculture and conserving agricultural lands. Based on soil and climate information, land suitability assessment for peaches (Prunus persica) and grapes (Vitis vinifera L.) were conducted using the maximum limiting characteristic method (MLCM) in South Korea. In peaches, S1 (highly suitable) exists on 2.21% of the land, S2 (moderately suitable) on 19.20%, N1 (currently not suitable) on 12.07%, and N2 (permanently not suitable) on the remaining 66.52%. In grapes, 3.65% of the land is classified as S1, 17.98% as S2, 11.85% as N1 and 66.52% as N2. In both fruit trees, the results acquired from soil and climatic information were similar to those from soil information alone. The data also suggest that the grades by soil information were relatively low over the land. With the assumption that the more suitable area a province has, the more will be cultivated for the fruit trees, we compared the percentages of area for peach and grape farming per province with the results by MLCM, and suggested that some provinces with a small percentage of farm can be encouraged to plant more in suitable areas as dictated by MLCM for the species. In the near future, we plan to use an advanced method such as analytic hierarchy process (AHP) to conduct similar tests, in which having reference data of yields or benefits per farm can efficiently increase the accuracy of the measurements.

Free Radical Scavenging Effect of Seed and Skin Extracts of Black Olympia Grape (Vitis labruscana L.) (거봉 포도 종자 및 과피 추출물의 항산화 활성)

  • Park, Sung-Jin;Oh, Deog-Hwan
    • Korean Journal of Food Science and Technology
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    • v.35 no.1
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    • pp.121-124
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    • 2003
  • Optimal extraction condition and free radical scavenging effect of ethanol extracts of Olympia grape (Vitis labruscana L.) seeds and skins were determined. Extraction yield of grape seeds increased with increasing extraction temperature increased, but was not affected by ethanol concentration in grape skins. Highest extraction yields obtained with 100% ethanol concentration at $78^{\circ}C$ were 36 and 49.5% in seeds and skins, respectively. Ethanol extract of grape seeds obtained at $78^{\circ}C$ showed stronger $RC_{50}\;(409.7\;{\mu}g/mL)$ than that of grape skins. Among organic solvent fractions, ethylacetate fraction obtained from ethanol extract of grape seed showed the strongest $RC_{50}\;(136.7\;{\mu}g/mL)$. Overall, ethanol extracts of grape seeds and their organic fractions showed significantly stronger free radical scavenging effect than those of skin extracts.

Growing features and Vegetation at natural growth area of ristics of Acanthopanax chiisanensis and Acanthopanax koreanum (지리산오갈피와 섬오갈피의 생육특성 및 자생지 식생조사)

  • 임병선;이점숙;김하송
    • Korean Journal of Plant Resources
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    • v.12 no.2
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    • pp.125-132
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    • 1999
  • This study has investigated the growing features and vegetation of Acanthopanax chiisanensis and Acanthopanax koreanum in Mt. Jiri and Halla from Sep. 1997 to Aug. 1998. As to Acanthopanax chiisanensis, it occupied surrounding areas of Nogodan in Mt. Jiri of the height of 1,507m, ImgeolyoungJae of 1,300m and the Mt. Halla. of 1,100m of There were rather high constancy degree with Quercus mongolica, Schizandra chinensis, Hydrangen serrate for. acuminata, Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Actaea asiatica, Thalictrum filamentosum, Abies koreana, Arundinella hirta from the sorts. The common species in Mt. Jiri and Halla are Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Callicarpa japonica, Parthenocissus tricuspidata, Acer mono, Ligularia fischeri, Disporum smilacinum, Viola acuminata Aconitum kusnezofii. On the other hand, concerning of Acanthopanax koreanum community, it possessed the small areas of wedo weoltae village in Chejudo. In this area, a liana e.g. Dioscorea tenuipes, Hedera rhombea, Lygodium japonicum, Akebia quinata, Vitis thunbergii, Dunbaria villosa, Trachelospermum asiaticum var. intermedium, Vitis amurensis, Paederia scandens, lives with Pinux thunbergii, Celtis aurantiaca, Mallotus japonicus and so forth in tree layer and subtree layer. There is artificial change no move in the researching sites, Acanthopanax chiixanensis will be transformed into Abies koreana - Acanthopanax chiisanensis community. Soil characteristics showed the high value such as pH 6.56 and conductivity 0.258mmho/cm in Acanthopanax koreanum community of Mt. Jiri, they also indicated digit as follows: the content of organic matter and total nitrogen are from 25.16 through 25.35% and 7.58 through 9.30mg/g respectably. The soil characteristics at Acanthopanax chiisanensis community in Mt. Jiri and Halla, was high as like pH 5.76, conductivity 0.238mmho/cm, total nitrogen 9.30mg/g, total phosphorus 0.126mg/g in Mt. Halla.

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Effects of Salt Concentrations on Accumulation of Pigments in Cell Suspension Cultures of Vitis vinifera and Phytolacca americana L. (포도와 미국자리공의 세포현탁배양계에 있어서 배지내 무기염 농도가 색소축적에 미치는 영향)

  • In, Jun Gyo;Lee, Young Bok;Choi, Kwan Sam
    • Korean Journal of Agricultural Science
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    • v.20 no.1
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    • pp.34-42
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    • 1993
  • Effects of salt concentrations on the cell growth and the pigment accumulation were investigated in cell suspension culture of Vitis vinifera and Phytolacca americana L.. The growth pattern of vine cell in control was showed the normal exponential growth pattern, but in the dilution media delay the exponential growth pattern from 4 to 8 days after culture. Maximal accumulation of anthocyanin was observed at 12 days after culture in all treatments. In cell suspension culture of Phytolacca, accumulation of betacyanin occurred in parallel with the cell growth pattern and maximal accumulation of betacyanin was observed after 8 days of culture. In the vine cell culture, the cell growth was showed the peak at 87.6mM of sucrose in the medium and reduced at over this concentration. Maximal anthocyanin accumulation was showed at 146mM of sucrose. In the higher concentrations of sucrose, the cell growth was rapidly decreased, but the accumulation of anthocyanin was not. Otherwise, in case of Phytolacca cell culture, betacyanin accumulation was showed in parallel with the cell growth increased with sucrose concentration. It was suggested that the anthocyanin of vine and the betacyanin of Phytolacca were controlled by different mechanisms.

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Properties of Wine from Domestic Grape, Vitis labrusca cultivar. Campbell's Early, Fermented by Carbonic Maceration Vinification Process (Carbonic maceration 발효 방법으로 제조된 국내산 포도, Vitis labrusca cultivar. Campbell's Early의 포도주 특성)

  • Park, Won-Mok;Park, Hyuk-Gu;Rhee, Sook-Jong;Kang, Kyung-Il;Lee, Cherl-Ho;Yoon, Kyung-Eun
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.773-778
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    • 2004
  • Properties of wine produced from domestic grape, cultivar Campbell's Early, by carbonic maceration vinification process (CM) were investigated. Process required whole grape clusters without crushing or destemming and no yeast inoculation for fermentation. Fermentation heat was not generated. Yield of wine was 77%, close to that obtained by conventional process, 76%. Acidities of CM wines, free run and pressed wines, and conventional wine were pH 3.6 and 3.3, respectively. Tartaric acid contents of conventional, free run, and pressed wines were 1,813, 4,691, and 5,633 ppm, while those of malic acid were 3,446, 2,077, and 2,275 ppm, respectively. CM could reduce malic acid content by 2/3 that of conventional process. Both processes gave almost equal amounts of citric and acetic acids. CM wines had intense grape aroma and deep purple-blue, natural grape color.

Physicochemical Property and Antioxidant Activity of Wild Grape(Vitis coignetiea) Juice (머루 주스의 이화학적 특성 및 황산화 활성)

  • Park, Hyun-Sil
    • Culinary science and hospitality research
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    • v.16 no.4
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    • pp.297-304
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    • 2010
  • This study examines the physicochemical property and antioxidant activity of wild grape juice, and the result is as follows. Total anthocyanin and color intensity contents of wild grape juice were $17.0{\pm}0.1%$ and $33.9{\pm}1.4%$, respectively. The contents of total phenols and flavonoid in wild grape juice were $25.4{\pm}3.1$ mg/100g, $4.4{\pm}1.4$ mg/100 g, respectively. Total mineral content in wild grape juice was $28.5{\pm}8.4$ mg/l00 g and the potassium content($11.3{\pm}0.3$ mg/100 g) was the highest Electron donating abilities of wild grape juice at concentration of $1,000\;{\mu}L$/mL were $94.8{\pm}1.2%$ Reducing power of wild grape juice at concentration of $1,000\;{\mu}L$/mL was 1.134. The electron donating abilities and reducing power were increased significantly with the sample concentration in the reaction mixture increased The nitrite scavenging ability was dependent on pH of the reaction mixture and sample concentration. The nitrite scavenging ability of wild grape juice was $77.1{\pm}1.1%$ at concentration of $1,000\;{\mu}L$/mL under pH 1.2.

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