• Korean Journal of Community Nutrition
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• v.7 no.6
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• pp.824-832
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• 2002

The present study was conducted to evaluate nutritional intake by lunch-box of middle school girls who live in Chonbuk area. Anthropometric measurements and food intake from lunch-box over a three day period were investigated in a total of 110 middle school girls from rural (n＝37) and urban (n＝73) areas. The results are summarized as follows : 1. Not all the steamed rice in the lunch-box had enough mixed-cereal. 2. In the survey of food intake by food groups, 70.5% of the food came from the cereal group. 3. The intake of protein, phosphate, Vitamins $B_1 and B_2$, niacine and Vitamin E were adequate, however, the intake of other nutrients was significantly lower than those of one-third of the Korean RDA.4. Protein, lipid, and zinc intakes of the students from the rural area were significantly lower than those of students from the urban area. however, the carbohydrate and dietary fiber intakes of those in the rural area were significantly higher than those of the students in the urban area. 5. The percentage of calories from carbohydrate : protein lipid ratio was 67.6 : 12.2 : 16.9 for students in the rural area and 59.2 : 14.9 : 22.5 respectively for those in the urban area. In Summary, the intake of calcium, iron, Vitamins A, $B_6$, and D, folate and zinc from lunch-box in middle school girls was much lower than that of one-third of the Korean RDA. The intake of carbohydrate and dietary fiber of the students in the rural area was significantly higher, but that of protein, lipids, and zinc was lower than that of the students in the urban area. Therefore, nutritional education programs for both students and their parents should be provided to improve the daily food intake from lunch-box.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1361-1366
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• 2008

One-hundred and twenty Ischia grey rabbits, traditionally raised in pits, were equally divided after weaning (32 days) into three groups: group C, housed in cages (4 rabbits/cage) and fed a commercial concentrate; group GF, housed as C group and fed grasses collected on the island and crushed faba beans supplemented with an appropriate mineral vitamin mix; group P, housed in pits (8 rabbits/pit) and fed as GF group. Feed intake was recorded daily and live weight monthly up to slaughter (92 days). At slaughter, 10 rabbits per group were used to measure carcass and meat traits. The carcasses were weighed and measured according to the standard procedures and meat samples from the Longissimus dorsi and left hind leg were analysed for water holding capacity and chemical composition, respectively. During the entire trial, group C consumed significantly (p<0.01) a higher quantity of feed than the other groups (126.1 vs. 63.4 and 66.5 g/d, resp. for groups C, GF and P) and at slaughter showed a significantly (p<0.01) higher body weight (2,529.7 vs. 1,324.4 and 1,375.4 g, resp. for groups C, GF and P). Significant differences (p<0.01) were found also for dressing out percentage (68.6 vs. 66.6 and 66.9%, resp. for groups C, GF and P) and for meat chemical composition, in particular lipid percentage (4.13 vs. 1.84 and 1.93%, resp., for groups C, GF and P, p<0.01) and moisture (73.7 vs. 76.4 and 76.3%, resp. for groups C, GF and P, p<0.01). The results suggest the opportunity to obtain heavier animals raised in the pits if their diets were integrated with commercial feed.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.4
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• pp.502-510
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• 2015

The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.

• Journal of Nutrition and Health
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• v.9 no.2
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• pp.87-97
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• 1976

This study is based on data from the food consumption survey on 727 members of 125 farm households from 7 different provinces. The survey was conducted in May, 1975 in cooperation with the O.R.D. The results obtained in this study are summarized as follows. 1. The average consumption of the basic food groups per capita per day was 563 g for cereals and grains(398g of rice and 129g of barley), 87.6g for meats and legumes, 317.8g for fruits and vegetables, 25.7g for milks and small fishes, 9.1g for fats and oils, and 45.1g for other group. 2. The average daily consumption of calories and nutrients was 2256 cal and 11.7g for animal proteins, 70.5g for total proteins, 21.6g for fats, 537.4mg for calcium, 18.1mg for iron, 5375lU for vitamin A, 1.27mg for thiamine, 1.05mg for riboflavin, 15.5mg for niacin, 77.7mg for ascorbic acid. When these figures are compared with the recommended allowances for Korean, the calories and nutrients intakes were satisfactory, except for the intakes of animal protein which was below two third of the recommended allowance. 3. The diets of the projected villages differed from those of the non-projected villages in the following respect: (a) The amounts of animal proteins and fats were larger in the projected villages than in the non-projected villages. (b) The percentage contribution of fats to the total amount of calories from three nutrients, carbohydrates, proteins and fats was higher in the projected villages than in tile non-projected villages. (c) The percentage contribution from carbohydrates to the total amount of calories was higher in the non-projected villages than in the projected villages. 4. Certain physical and clinical symptoms were observed among the people in the rural areas, which can be related to the shortages of animal proteins and fats in their diets. It is recommended to pay special attention to the nutrition of school children in the Korean rural areas.

• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.62-69
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• 1999

The present study was aimed at investigating whether there are sex-related differences in serum levels of lipids, retinol , $\alpha$-tocopherol and ascorbic acid in Korean adults. Serum levels of antioxidant vitamins and lipids were determined algon with anthropometric measurements in 53 healthy male subjects with mean age 42.7$\pm$12.2 years and 44 female subjects with mean age 46.7$\pm$10.3 years. from Taegu in Korea. BMI and W/H ration of the men were 22.66$\pm$2.47, 0.88$\pm$0.04 and those fo thewomen were 23.36$\pm$3.44, 0.84$\pm$0.05 respectively. Systolic and diastolic blood pressures were 121.67$\pm$14.8, 79.3$\pm$12.4 in the men and 123.1 $\pm$17.5 , 78.8$\pm$10.9 in the women respectively. Average serum levels of total cholesterol, LDL-and HDL-cholesterol and triglyceride were 165.8$\pm$36.4, 102.6$\pm$31.2, 41.1$\pm$10.5 and 110.2$\pm$57.8mg/dl in the and 169.1$\pm$39.1, 113.7$\pm$36.2, 38.1 $\pm$8.6, and 85.2$\pm$37.7mg/dl in the women respectively. Thirteen percent of menans thirtyeight percent of women had LDL-cholesterol over 130mg/dl. Serum levels of retinol, $\alpha$tocopherol, ad ascorbic acid were 43.25 $\pm$15.51, 17.93$\pm$7.07, 115.24$\pm$63.25ug/dl in the men and 31.80$\pm$15.39, 17.41$\pm$6.12, an d144.99$\pm$89.87ug/dl in the women respectively. Serum vitamin E showed positive correlations with total cholesterol and LDL-cholesterol and triglyceride.

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.472-475
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• 1998

A female Green iguana (Iguana iguana) was brought to the small animal clinic in Hannover Veterinary school because of anorexia and convulsion. Two months ago, the owner bought two iguanas, one female and one male and had to take a female to the one of local small animal clinic because she, born in 19931 didn't eat well and showed the tremor of the legs. The veterinarian suspected it to have a calcium deficiency and gave an injection of calcium and vitamin D3. But still during the last two months, the female iguana did only eat some lettuce while the other male ate lettuce, fruits and commercial cat-food ($Whiskas^{\circledR}$, Effem, Verden). It was not possible to evaluate the rations of these different "food-components". They are kept in the terrarium with water container. By palpation of the abdomen, hard thing like stone was palpated in the mid-region. The blood test wasn't carried out because of the difficulties of getting blood and because of her bad condition. It was firmly diagnosed as cystic calculus through radiography. Since the animal is in shock state already, the prognosis after surgery was bad. It was assumed to be too late to expect its surviving with the surgery carried out or without it. Cystotomy was performed in the cranial ventral paramedian incision in order to preserve the large ventral abdominal vein in the linen alba region. After two stay sutures ("holding suture" ) with 5-0 polyglactin 910 ($Vicryl^{\circledR}, Ethicon$) on the bladder, cystotomy was carried out and the stone inside was removed. Then, the bladder was closed in one-layer sutures. The abdomen was closed in simple interrupted suture with same material after the instillation of 10 ml saline solution into the abdominal cavity. The cystic calculus was in a size of $5.8{\times} 5.O{\times} 3.5 cm$. The analysis of the stone was carried out in two ways which are the infrared spectroscopy of the stone and quick analysis by $Merckognost^{\circledR}$(Diagnostica Merck, Merck). In the result of the infrared spectroscopy, the stone wee composed of 10% ammonium urate and 90% dihydrated uric acid. This iguana wasn't able to survive.n't able to survive.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.30 no.3
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• pp.313-320
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• 2020

Objectives: The aim of this study is to evaluate the association between vibration exposure and skeletal muscle mass index through a single university health check-up. Methods: We used data from 134,067 male subjects who received a general health check-up or vibration exposure health check-up out of the 1,515,322 people who underwent medical check-up at a local university hospital from 2002 to 2018. Pearson correlation analysis was conducted for comparing the association between skeletal muscle mass index and demographic and hematological variables in both groups. Mixed linear model analysis after controlling demographic and hematological variables was used to analyze the differences in skeletal muscle mass index between groups at every visit over 10 years. Results: In the Pearson correlation test, the variables that showed different results when comparing the two groups were C-reactive protein (p=0.001) and glycated hemoglobin (p=0.002) in the vibration exposure group and erythrocyte sedimentation rate (p<0.001) and vitamin D (p<0.001) in the general group. After the adjustment of demographic and hematologic variables, the skeletal muscle mass index at every visit was markedly decreased in the vibration exposure group (p<0.001). Conclusions: In the vibration exposure group, the skeletal muscle mass index showed a tendency to decrease markedly over time compared to the general health check-up group, which showed that C-reactive protein and glycated hemoglobin would have an influence on skeletal muscle index in male workers exposed to vibration.

• Korean Journal of Head & Neck Oncology
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• v.15 no.1
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• pp.52-60
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• 1999

Objectives: For investigation of the differentiation between transient and permanent hypocalcemia, we focused on a postoperative calcium requirement and an interval of normalization in serum hypocalcemic level and studied for the causes of postoperative hypocalcemia. Material and Method: Postthyroidectomy hypocalcemia was studied in 193 patients who were admitted from January, 1991 to December, 1998 and underwent lobectomy, subtotal thyroidectomy or total thyroidectomy. We compared postoperative serum calcium, phosphate and ionized calcium levels among three groups which were lobectomy, subtotal thyroidectomy and total thyroidectomy, respectively. Result: All patients revealed postoperative decline in serum calcium and ionized calcium, especially, the lowest serum calcium level was seen in 48 hours after surgery. Serum calcium level was returned to normal in five to six postoperative days in most patients. But 24 patients required calcium supplementation due to symptomatic hypocalcemia. In this series, we discovered that the important period for monitoring of serum calcium level was 24 to 96 hours after surgery. If the calcium replacement therapy was not required in the first 72 hours after surgery, it would not be needed during the remainder of the patient's hospital course. Symptomatic transient hypocalcemia was 22 cases(11.4%) and permanent hypocalcemia was 2 cases(1%). Conclusion : We found that hypoalbuminemia, preoperative hyperthyroidism and impairment of blood supply to parathyroid were the main causes of postthyroidectomy hypocalcemia. We also thought that the interval from initial medication to normalization in serum calcium level, and the increase of requirement in calcium and vitamin D were the important factors for differentiation between transient and permanent hypocalcemia.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.203-209
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• 2005

This study was conducted to improve tocopherol (vitamin E) composition in soybean (Glycine max) by introducing a gamma-tocopherol methyl transferase (${\gamma}$-TMT) gene via Agrobacterium tumefaciens-mediated transformation. Immature cotyledon explants were cocultivated with Agrobacterium tumefaciens. Putative transgenic embryos were selected from immature cotyledons on MS medium supplemented with 40 mg/L 2,4-D containing 100 mg/L kanamycin, 500 mg/L carbenicillin and 250 mg/L cefotaxime. Plantlets were developed from somatic embryos, and then transferred to soil. Nineteen regenerated plantlets obtained on the selection medium from 1,460 cotyledons. However, only 9 plantlets were confirmed as transformed plants. Integration of the transgene into the soybean genomic DNA was confirmed by PCR and Southern blot analysis. HPLC analysis showed that the content of ${\alpha}$-tocopherol in transgenic soybean seeds (AT-1) was approximately 4-fold higher than that of non-transgenic plants. Conclusively, we obtained the transgenic soybean having increased ${\alpha}$-tocopherol content by the overexpression of ${\gamma}$-TMT transgene.

• Journal of Pharmaceutical Investigation
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• v.39 no.4
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• pp.309-314
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• 2009

An enzyme immunoassay (EIA) was validated for quantitation of cacitriol in human plasma. Calcitriol was immunoextracted with immunocapsules, which contain monoclonal antibodies to calcitriol linked to solid phase particles in suspension with a vitamin D binding protein inhibitor. Calcitrol was eluated and the eluates were evaporated under a gentle stream of nitrogen gas. The absorbance of analytes was determined using a microplate reader (reference wavelength 650 nm; measurement wavelength 450 nm). The method was specific and sensitive enough to detect as low as 6.5 pmol/L of calcitriol. Linear calibration range was 6.5-491 pmol/L with correlation coefficient greater than 0.99. The overall accuracy was in the range of 83.8 to 111.2% and precision C.V. (%) 0.99 to 8.47%. The recovery was approximately 100% and stability was confirmed during storage and sample preparation. The pharmacokinetic parameters were calculated by baseline subtraction because calcitriol is an endogenous material. Following oral dose of calcitriol, the mean AUC$_{24h}$ was 1038${\pm}$539 pmol/Lhr and C$_{max}$ of 128${\pm}$63.1 pmol/L was reached at 3.50${\pm}$1.07 hr. The mean t$_{1/2}$ of calcitriol was 5.13${\pm}$2.10 hr. The present EIA method was successfully applied to study bioavailability after oral administration of 2 ${\mu}$g of calcitriol in healthy Korean subjects.