• 농업생명과학연구
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• 제45권4호
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• pp.121-133
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• 2011

Effects of roasting on vitamin E content, color, microstructure and moisture of peanuts, and vitamin E content in peanut oils prepared from the roasted peanuts were investigated. Runner-type peanuts were roasted at 140, 150, and $160^{\circ}C$ for 10-20 min. As roasting temperature and time increased, the CIELAB $L^*$ value of peanuts decreased while $a^*$ and $b^*$ values increased, resulting in formation of the golden brown color of roasted peanuts. Moisture ratio (M/Mo) and color $b^*$ value of peanuts roasted at 140 to $160^{\circ}C$ showed a correlation of $b^*=21.61\;(M/Mo)^2-40.62\;(M/Mo)+34.12$ ($R^2=0.9123$). Overall changes in the tocopherol contents of peanuts and peanut oils were significantly affected by roasting temperature and time (p<0.05). Roasting at $140^{\circ}C$ caused a slight increase in the levels of tocopherols of peanuts over roasting time up to 20 min (p<0.05). There was no significant change in the tocopherol levels of peanuts during roasting at $150^{\circ}C$ for 20 min (p>0.05). At $160^{\circ}C$, the levels of tocopherols significantly decreased during the initial 10 min of roasting (p<0.05) while there was no extended loss after 10 min, resulting in about 5, 12, 20, and 10% losses of ${\alpha}$-, ${\beta}$-, ${\gamma}$- and ${\delta}$-T, respectively. After 20 min, total tocopherols decreased by 18%. However, tocopherol contents of pressed peanut oils significantly decreased at all roasting temperatures (p<0.05). After roasting peanuts at $160^{\circ}C$ for 20 min, about 84% of initial ${\alpha}$-T in peanut oils was retained. ${\alpha}$-T was the most stable to roasting while ${\gamma}$-T was the least. Swollen epidermal cells on the inner surface and broken cell walls of parenchyma tissue of peanut cotyledon were observed in peanuts after roasting at $160^{\circ}C$ for 15 min. Severe changes in microstructure of peanut by roasting would contribute to vitamin E stability because of exposure of oil droplets in peanuts to oxygen.

• 한국식품과학회지
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• 제30권2호
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• pp.272-277
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• 1998

가공식품 원료 및 생식용 과일로서 생대추의 저장기간을 연장시키고자 $7{\pm}1^{\circ}C$에서의 CA저장과 $1,\;4,\;7{\pm}1^{\circ}C$의 온도조건에서 무포장 및 0.05 mm PE포장을 처리하여 저장하면서 품질변화 및 이화학적 성분 변화를 조사한 결과는 다음과 같다. 신선도로 본 생대추의 안전 저장 기간은 $1^{\circ}C$, PE film 포장에서 4주 였고, CA 처리구에서는 $O_2\;10%,\;CO_2\;1.5%$ 처리에서 선도가 가장 잘 유지되었다. 중량 감소는 CA처리구와 PE처리구 모두 거의 차이가 없었고 무포장 처리구에서는 저장 4주째부터 5% 내외의 중량 감소를 보여 생과로서는 상품 가치가 없었다. 색도는 저장 기간이 경과함에 따라 모든 처리구에서 L과 b값은 감소되고, a값은 증가하는 경향을 보였다. 경도는 전반적으로 저장 2주까지는 모두 증가하는 경향이었으나 CA처리구의 경우 저장 4주째 부터는 PE 처리구 및 무포장 처리구에 비해 연화가 더 느리게 진행됨을 보였다. 온도차이에 의한 경도 변화는 저온일수록 연화가 느리게 진행됨을 보였다. 초기 생대추의 당도와 적정 산도는 $26.6^{\circ}brix,\;1.18%$였으며 당도와 산도의 변화는 저장 기간 중 큰 변화가 없었다. 저장 초기 생대추의 Vitamin C 함량은 148.09 mg% 였고 저장기간에 따른 함량변화는 $1^{\circ}C$ 무포장 처리구를 제외한 모든 처리구에서 점차 감소되었으며 다른 처리구에 비해 CA 처리구에서 Vitamin C의 소실이 적어 더 안정함을 알 수 있었다.

• 대한화장품학회지
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• 제50권2호
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• pp.103-110
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• 2024

지질나노입자(lipid nanoparticles, LNPs)란, 세포 불투과성인 치료용 핵산, 단백질 및 펩타이드와 같은 생물학적 활성 화합물을 미세한 환경 변화에 의한 분해로부터 보호해 안정적이면서 효과적인 약물전달시스템이다. 본 연구에서는 지용성 비타민 C 유도체인 테트라헥실데실아스코르베이트(tetrahexyldecyl ascorbate, THDC)의 생체 내 반감기를 효과적으로 연장시키기 위해 산화방지제인 갈릭산(gallic acid, GA)을 함유하는 지질나노입자를 개발하였다. 마이크로플루이딕스칩(microfludics chip)을 통해 상온 및 상압 조건 하에서 작고 균일한 크기로 제작하였다. 기존 마이크로플루다이저(microfluidizer)를 통해 고압 및 고온 조건 하에 제작된 리포좀과 비교하였을 때, 마이크로플루이딕스칩(microfludics chip)을 통해 제작된 LNPs인 경우, 분산 및 온도에 따른 안정성이 우수하였으며, 지용성 비타민 C 유도체의 산화 안정성을 향상시켰을 뿐 아니라 피부 흡수율도 개선된 것을 확인하였다. 향후 연구에서는 이러한 결과를 더욱 입증하기 위해 피부 개선 효과를 연구하기 위한 생체 외 및 생체 내 평가에 중점을 둘 것이다.

• 한국식품영양과학회지
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• 제42권7호
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• pp.1086-1095
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• 2013

본 연구에서는 화피 추출물을 첨가하여 제조한 개성주악에 대해 합성 항산화제를 첨가한 개성주악과 그 산화안정성을 비교하면서 저장기간($60^{\circ}C$, 4일) 동안 항산화 활성 및 품질 특성의 변화를 측정하였다. 개성주악의 색도 측정 결과 화피 추출물 첨가량의 증가, 저장기간의 증가에 따라 L값은 감소하고 a값과 b값이 높아지는 경향을 나타냈다. 조직감 측정결과에서는 화피 추출물의 첨가는 개성주악의 경도와 응집성을 높이고 부착성을 감소시키는 경향을 나타내었으며, 저장기간에 따른 변화에서도 유사한 경향을 나타냈다. 관능검사 결과 색, 맛, 전반적인 기호도에서 0.5, 1% 첨가군이 높은 점수를 나타냈으며, 이러한 경향은 저장기간 동안 유사하게 나타났다. 화피 추출물 첨가군의 산가, 과산화물가, 아니시단가는 무첨가군과 비교할 때 저장기간 동안 낮게 보여졌으며, 0.02% vitamin C와 0.02% BHT 첨가군의 경우 1, 2% 첨가군과 유사한 산화안정성를 나타내었다. 화피 추출물 첨가 개성주악의 항산화 활성 측정 결과, 시료의 첨가량에 비례하여 그 활성이 유의적으로 증가하였으며 이러한 경향은 저장기간 동안 유지되었다. 이상의 결과에서 개성주악에 대한 화피 추출물의 첨가는 개성주악의 기호도 및 산화안정성, 항산화활성을 증가시켜 개성주악의 가치를 높일 수 있어 바람직할 것으로 사료되며, 개성주악 제조 시 기호도가 높고 산화안정성, 항산화능이 좋았던 0.5~1% 화피 추출물을 첨가하는 것이 적합할 것으로 생각된다.

• Bulletin of the Korean Chemical Society
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• 제32권8호
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• pp.2648-2656
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• 2011

A simple HPLC-DAD method was developed and validated to determine B group vitamin content (thiamine, riboflavin, nicotinamide, pantothenic acid, pyridoxine and folic acid) in supplemented food samples, i.e., infant formula, cereal, low-calorie food, a multi-vitamin pill and a vitamin drink. In this study, the most significant advantages were simultaneous determination of the six B group vitamins in various food matrices and a small number of sample treatment steps that required only an organic solvent, acetonitrile. Moreover, this method prevents reduction of column durability, because the mobile phase does not contain ion-pairing reagents. Analytes were separated on a Develosil RPAQUEOUS $C_{30}$ (4.6 mm ${\times}$ 250 mm, 5 ${\mu}M$ particle size) column with a gradient elution of acetonitrile and 20 mM phosphate buffer (pH 3.0) at a flow rate between 0.8 and 1.0 mL/min. Detection was performed at 275 nm, except for that of pantothenic acid (205 nm). The calibration curves for all six vitamins showed good linearity with correlation coefficients ($r^2$) higher than 0.995. The developed method was validated with respect to linearity, intra- and inter-day accuracy and precision, limit of quantification (LOQ), recovery and stability. The method showed good precision and accuracy, with intra- and inter-assay coefficients of variation less than 15% at all concentrations. The recovery was carried out according to the standard addition procedure, with yields ranging from 89.8 to 104.4%. This method was successfully applied to the determination of vitamin B groups in supplemented food products.

• 한국식품영양과학회지
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• 제45권9호
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• pp.1316-1323
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• 2016

저속압착방식의 주서기를 이용하여 착즙한 파인애플 주스와 키위 주스를 냉장($4^{\circ}C$) 및 냉동온도($-20^{\circ}C$)에서 30일 동안 저장하면서 저장조건이 주스의 항산화 활성 및 단백질 분해효소 활성에 미치는 영향을 확인하였다. 비타민 C 함량 및 총폴리페놀 함량은 파인애플 주스, 키위 주스 모두 냉동저장에서는 30일까지 90% 이상이 유지되었으나 냉장저장에서 비타민 C 함량은 각각 56.8% 및 32.9%, 총폴리페놀 함량은 각각 31.9% 및 22.4%가 감소하였으며, DPPH 라디칼 소거능도 냉동조건보다는 냉장조건에서 활성 감소율이 크게 나타나 유사한 결과를 보였다. 활성 감소율은 초기 비타민 C의 함량이 파인애플 주스보다 1.7배 더 높은 키위주스에서 낮게 나타났다. 파인애플 주스와 키위 주스의 단백질분해효소 활성은 냉동저장 30일까지 모두 초기 활성의 90% 이상으로 높게 유지되었으며 냉장저장 30일 후 파인애플 주스는 초기 활성의 88.4%를 유지하였지만 키위 주스는 초기 효소 활성의 34.5%만이 남아있어 서로 다른 양상을 나타내었다. 이러한 결과는 단백질분해효소 활성을 육안으로 직접 확인하기 위해 진행한 닭 가슴살 단백질분해 활성 측정 결과에서도 확인할 수 있었다. 이상의 결과로부터 천연 연육제 또는 소화보조제로 널리 이용되는 파인애플 및 키위주스의 보관 시 냉장저장보다는 냉동저장이 항산화 활성 및 단백질분해효소 활성의 유지 측면에서 바람직한 것으로 사료된다.

• 한국식품저장유통학회지
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• 제2권2호
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• pp.243-249
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• 1995

Potatoes, Irish cobbler, were subject to quality evaluation from the physiological and physicochemical points of view during storage under different temperatures and relative humidities for 11 months. Quality criteria were sprouting, rotting, weight changes, moisture, total sugar, reducing sugar and vitamin C. Low temperature condition(LT, 2∼4$^{\circ}C$, 80% RH) was enough to preserve Potatoes for 11 months, but it was indicated to be limitations that the increase in reducig sugar as well as sprouting by about 3.3 to 6.7% occurred from the 3rd month of storage. Meanwhile, pit temperature(PT, 3∼15$^{\circ}C$, 75∼85% RH), room temperature(RT, 7∼17$^{\circ}C$, 80∼95% RH) and ambient temperature(AT, 2∼25$^{\circ}C$, 62∼72 RH) were shown infeasible conditions for the storage of potatoes mainly due to the increase In sprouting-rate and subsequent quality-loss after 3 months of storage. Based on the results, it proposed that more economical conditions, ranging 10 to 15$^{\circ}C$ and about 80% RH following sprout inhibition treatment should be used for the long-term storage of potatoes.

• Journal of Nutrition and Health
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• 제18권1호
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• pp.36-45
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• 1985

The effects of glucose, lactose, sucrose, and potato starch on kimchi fermentation and on the stability of ascorbic acid were investigated at $7^{\circ}C$. Kimchi samples with the sugars showed the higher PH and lower total acidity until 9th day of fermentation than kimchi without sugar. Changes in the pH and total acidity were not significantly different among the samples after the 12th day. Addition of the sugars did not show any effects on the growth of lactobacilli through the fermentation period. The amounts and changes in ascorbic acid content during the fermentation did not differ significantly between the control and sugar - added samples. Ascorbic acid in most samples decreased continuously by the 9th day of fermentation. After then the vitamin increased in all the samples and then again decreased slowly after 18th day. From the multiple comparison tests the 10- panel members indicated the better flavor of sugar - added samples than control sample. However the differences in flavor were not significant.

• Journal of Pharmaceutical Investigation
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• 제31권3호
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• pp.167-172
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• 2001

All-trans retinoic acid (ATRA), vitamin A acid, has been shown to exert anticancer activity in a number of types of cancers, particularly in acute promyelocytic leukaemia (APL). Due to its highly variable bioavailability and induction of its own metabolism after oral treatment, development of parenteral dosage forms are required. However, its poor aqueous solubility and chemical unstability give major drawbacks in parenteral administration. This study was undertaken to investigate a possibility to develop a parenteral formulation of ATRA by employing solid lipid nanoparticle (SLN) as a carrier. By optimizing the production parameters and the composition of SLNs, SLNs with desired mean particle size (<100 nm) as a parenteral dosage form could be produced from trimyristin (as solid lipid), Egg phosphatidylcholine and Tween 80 (as SLN stabilizer). The mean particle size of SLN formulation of ATRA was not changed during storage, suggesting its physical stability. Thermal analysis confirmed that the inner lipid core of SLNs exist at solid state. The mean particle size of ATRA-loaded SLNs was not significantly changed by the lyophilization process. ATRA could be efficiently loaded in SLNs, while maintaining its anticancer activity against HL-60, a well-known APL cell line. Furthermore, by lyophilization, ATRA loaded in SLN could be retained chemically stable during storage. Taken together, our present study demonstrates that physically and chemically stable ATRA formulation adequate for parenteral administration could be obtained by employing SLN technology.

• 약학회지
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• 제41권5호
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• pp.595-601
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• 1997

The optimal synthesis condition based on the yield of thiamine mononitrate has been attained according to Box-wilson experimental design. The optimal condition was as follows : Molar concentration ratio of $SB1{\cdot}HCL=0.6839$, quantity of $NH_4NO_3=50.09g$, agitation velocity=51.6rpm, reaction temperature=22.8$^{\circ}C$. The stabilities of three kinds of thiamine monosalts(thiamine monoiodide, -monobromide, -monorhodanate) were investigated in sterile solution, compressed tablet and multivitamin capsule, respectively. Assay data are given to show that thiamine monosalts are not significantly higher than that of thiamine mononitrate in sterile solutions and the addition of an acidic stabilizer is unnecessary. The solutions were clear and free of precipitate and didn't discolored after storage at room temperature and 40$^{\circ}C$ for 6 months. The tablets and capsules were assayed initially and at predetermined intervals during storage at room temperature and 40$^{\circ}C$ for 6 months. Thiamine monorhodanate was found to be more stable than other thiamine monosalts in these compressed tablets containing other vitamin of the B complex and the data indicate that thiamine monosalts was similar to that of thiamine mononitrate in the multivitamine capsules.