• Title/Summary/Keyword: Visualized Data

검색결과 567건 처리시간 0.029초

인체 SIP 단백질에 특이적인 단일클론 항체의 특성 (Characterization of a Monoclonal Antibody Specific to Human Siah-1 Interacting Protein)

  • 윤선영;주종혁;김주헌;강호범;김진숙;이영희;권두한;김창남;최인성;김재화
    • IMMUNE NETWORK
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    • 제4권1호
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    • pp.23-30
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    • 2004
  • Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

3-Benzylidine/Furfurylidine/(Pyridyl/Thienyl-2'-methylene) imino-5-p-sulphonamido phenyl azo-2-thiohydantoins와 UO2(VI), ZrO(IV) 및 Th(IV) 이온의 동종이핵 착물에 대한 합성 및 특성 (Synthesis and Characterization of Homobinuclear Complexes of UO2(VI), ZrO(IV) and Th(IV) ions with 3-Benzylidine/Furfurylidine/(Pyridyl/Thienyl-2'-methylene) imino-5-p-sulphonamido phenyl azo-2-thiohydantoins)

  • Dash, D.C.;Mahapatra, A.;Naik, P.;Mohapatra, R.K.;Naik, S.K.
    • 대한화학회지
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    • 제55권3호
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    • pp.412-417
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    • 2011
  • [$M_2L(NO_3)_n(H_2O)_m$]의 일반식을 갖는 일련의 동종이핵 착물을 합성하여 원소분석, 열분석, 몰 전기전도도, 자기모멘트 및 분광학적 방법(IR, UV-vis 및 $^1H$-NMR)을 통해 특성을 조사하였다. 이 경우, 일반식에 있어서 M=$UO_2$(VI), ZrO(IV), Th(IV); L=3-benzylidine-imino-5-p-sulphonamido phenyl azo-2-thiohyatoin(bispt), 3-furfurylidine-imino-5-psulphonamido phenyl azo-2-thiohydantoin(fispt),3-pyridyl-2'-methylene-imino-5-p-sulphonamido phenyl azo-2-thiohydantoin (pmispt) 및 3-thienyl-2'-methylene-imino-5-p-sulphoanamido phenyl azo-2-thiohydantoin(tmispt); Th(IV)에 대해서 n=8 그 외의 것에 대해서는 n=4, bispt에 대해서는 m=4 그 외의 것에 대해서는 m=3을 의미한다. 이러한 결과에서 얻은 정보를 통해 리간드들은 4배위를 하는 것으로 확인되었는데, 즉 한 중심금속은azomethine 질소 및 카르보닐 산소에 배위되는 반면, 또 다른 중심금속은 아조 질소 및 thioimido 질소에 배위되어 위의 조성을 갖는 동종이핵 착물을 이룬다. 몇 가지 병원균에 대한 리간드와 그들의 지르코닐 착물의 독성 연구도 수행하였다.

3차원 재구성법에 의한 C-shaped root의 형태분석 (MORPHOLOGIC ANALYSIS OF C-SHAPED ROOT USING 3-D RECONSTRUCTION)

  • 정은희;신동훈
    • Restorative Dentistry and Endodontics
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    • 제27권4호
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    • pp.421-431
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    • 2002
  • C-shaped canal configuration is very difficult to treat because that clues about preoperative canal anatomy cannot be ascertained from clinical crown morphology and limited information can be derived from radiographic examination. This study was done to get more informations about the root and canal configuration of C-shape root by 3-dimensionally reconstructing for the purpose of enhancing success rate of endodontic treatment. 30 mandibular molars with C-shaped root were selected. Six photo images from occlusal, apical, mesial, distal, buccal, lingual directions and radiographic view were taken as preoperative ones to compare them with 3-D image. After crown reduction to the level of 1-2mm over pulpal floor was performed, teeth were stored in 5.25% sodium hypochlorite solution for the removal of pulp tissue and debris. They were cleaned under running water, allowed to bench dry and embedded in a self-curing resin. This resin block was serially ground with a microtome (Accutom-50, Struers, Denmark) and the image of each level was recorded by digital camera (FinePix S1-pro, Fuji Co., Japan). The thickness of each section was 0.25mm. Photographs of serial sections through all root canal were digitized using Adobe Photoshop 5.0 and then minimum thickness of open and closed sites were measured (open site is the surface containing occluso-apical groove closed site is oppsite). After dizitization using 3-D Doctor (Able software Corp, USA). 3D reconstruction of the outer surface of tooth and the inner surface of pulp space was made. Canal classsification of C-shaped roots was performed from this 3-D reconstructed image. The results were as follows : 1. Most C-shape rooted teeth showed lingual groove (28/30). 2 According to Vertuccis' calssification, type I, II, III, IV, VII were observed. but also new canal types suck as 2-3-2, 1-2-3-2. 2-3-2-1, 2-3-2-3 were shown. 3 There was little difference in minimum thickness on coronal and apical portions, but open site were thinner than closed site on mid portion. Conclusively, 3D reconstruction method could make the exact configurations of C-shape root possible to be visualized and analyzed from multi-directions. Data from minimum thickness recommend cleaning and shaping be more carefully done on dangerous mid portion.

전침자극(電針刺戟)에 의한 흰쥐 중추신경계(中樞神經系)내 대사활성(代謝活性) 변화(變化)의 영상화(映像化) 연구(硏究) (2-DG Autoradiographic Imaging of Brain Activity Patterns by Electroacupuncture Stimulation in Awake Rats)

  • 손영주;원란;정혁상;김용석;박영배;손낙원
    • Journal of Acupuncture Research
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    • 제18권3호
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    • pp.56-68
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    • 2001
  • Objective : Functional brain mapping study on acupuncture stimulation using the [14C]2-deoxyglucose([14C]2-DG) autoradiography provides quantitative data and visualized pathway in central nervous system(CNS). We aimed to investigate the neural pathway and spatial distribution of metabolic activity elicited in CNS on electroacupuncture stimulation using [14C]2-DG autoradiography. Methods : The study were divided into three groups by stimulation times. 45-mins stimulation group according to Sokoloffs method, 5-mins stimulation group according to Duncun's method, and 15-mins stimulation group. ;A venous catheter was equipped into right jugular vein. The rats (Sprague-Dawley rats, 230-260g) were kept fastened loosely on a holding platform without anesthesia. Electroacupuncture stimulation (5 ms, 2 Hz, 1~3 mA) were applied on the left Zusanli (ST36) acupoint and [14C]2-DG ($25{\mu}Ci/rat$) injection was performed through the catheter. After sacrifice, the brain and the spinal cord were made to sections for film image. The film images were digitalized as the isotope concentration based upon comparison of optical densities with that of the standards and normalized by the optical density of corpus callosum. Results : 1. 15-mins stimulation group was most effective among 3 experiments. 2. On 15-mins stimulation group, medial geniculate nucleus, intetpeduncular nucleus intermedius, ventral periolivary nucleus, caudal periolivary nucleus, medial superior olive, lateral paragigantocellular nucleus, including hypothalamic arcuate nucleus were increased by more than 25% (at least, p<0.05) by electroacupuncture stimulation. 3. Especially, the metabolism in hypothalamic arcuate nucleus was increased by 90% (p<0.05). 4. The fact that arcuate nucleus of hypothalamus might play a role of interconnection area between ascending and descending pathway of acupuncture stimulation was demonstrated visually. Conclusions : Advanced study on electroacupuncture stimulation elicited significant increase of metabolic activity in various nuclei of hypothalamus will provide the important experimental basis in research of the relationship between electroacupuncture stimulation and internal visceral functions.

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여성건강을 위한 개념적 모형 (Conceptual Model for Women s Health)

  • 이경혜
    • 대한간호학회지
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    • 제27권4호
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    • pp.933-942
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    • 1997
  • There has recently been an increased interest in women's health from, various disciplines, with different perspectives presented according to each profession's academic background. This has led to many instances of incorrectly defining, or misinterpretation, of the issues even among professionals. Nurse scholars as well as practitioners who work in women's health care need to have a clear conceptual understanding of women's health in order to build a body of knowledge, delineate curricular activities, and set directions for professional nursing interventions. In addition, a conceptual model that may be directly utilized in practice is needed to maintain and promote women's health issues. The purpose of this study was to apply a Hybrid model, analyzing conceptual definitions and discussions related to women's health gathered from review of the literature. Further to compare analyticals the concepts and properties observed from field work, so as to present a final definition of women's health and, build a conceptual framework for a united comprehensive perspective on the concept as well as on nursing practice. Data collection and analysis consisted of a theoretical stage, field work stage, and final analysis. A heterogeneous group of professionals and lay persons, 39 in all, participated in the field work. Study findings Include several subconcepts under the concept of women's health : a woman's whole life, holistic health, quality of life, awareness of being a woman, individual nursing, self care ability, reproductive health, and family health. Thus, a comprehensive definition was built, 1. e., "Women's health care be defined as improvement in the quality of life of women through attainment of holistic health throughout the life span. With reproductive health at the core, the concept is directly related to family and national health, and includes taking care of one's own health based on awareness of being a woman and utilizing self care activities. Women's health care issues are unique and allow various responses, therefore women's health professionals need to apply individual approaches to reach solutions in attaining holistic health and improving quality of life." The constructual factors of women's health were found to be reproductive functions, diseases more common in woman, self actualization, mental health, women's health policies, sexuality, midlife changes, and marital relations, with each factor having more than three properties. Positive factors affecting women's health were found to be a normal childbearing process, a healthy lifestyle, active health management, health information, support, and resources, and interpersonal relationships. Negative factors were found to be overwhelming role stress, cultural oppression, gender inequality, distorted sexual identity, economic difficulties, misuse and/or abuse of substances, and stress. The model of women's health may be visualized as a balance scale set upon a woman's life, supporting 4 concentric circles. The innermost circle and second circle incorporate conceptual definitions of women's health, and the outer two circles represent the constructional factors and properties of women's health. Each circle has its own color that symbolizes the conceptual meaning. Positive and negative factors are represented as weights at either end of the scale, and are affected by nursing intervention, i. e., health and wellness increase when positive factors are stronger, whereas disease and illness increase when negative factors are stronger. This model is only a preliminary effort and requires much discussion and testing to be further developed. Continuous research is also required.

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건축기획단계 지원을 위한 BIM 기반 공간계획 시뮬레이션 시스템 개발에 관한 연구 (A Study on the Development of a BIM-based Spatial Planning Simulation System for Architectural Planning Stage Support)

  • 최선영;최주원;김주형;김재준
    • 한국BIM학회 논문집
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    • 제1권2호
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    • pp.19-23
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    • 2011
  • 건축 프로젝트의 기획단계는 타 단계에 비해 프로젝트의 비용이나 성과에 미치는 영향력이 매우 크다. 또한 최근 건설프로젝트의 대형화 복합화 추세에 따라 건축 기획의 중요성은 더욱 증가하고 있다. 그럼에도 현재 기획단계의 업무는 체계적으로 관리되지 못하고 있는 실정이다. 이에 본 연구는 공간계획 및 검토, 비용검토, 발주자 요구사항 관리 등의 건축기획단계 업무를 지원하기 위한 BIM 기반의 시뮬레이션 시스템을 개발하는 것을 목적으로 하였다. 본 시스템은 공간 객체의 모델링뿐만 아니라, 객체 정보를 기반으로 하여 계획의 변경에 따른 공간면적 및 배치, 비용 등을 즉각적으로 검토할 수 있어 여러 대안의 검토가 용이하다. 또한 건축물 모델의 시각화된 정보와 정량적인 데이터를 제공함으로써 발주자와의 의사소통 도구로 사용할 수 있으며, 본 시스템에서 생성된 정보를 후속 단계로 전달하여 활용이 가능하다. 시스템을 이용하여 건축기획단계를 비롯한 프로젝트 전체의 업무를 지원하고 나아가 건축 프로세스의 선진화에 기여할 수 있을 것으로 생각된다.

게이트 심근 SPECT에서 좌심실의 운동성 분석을 위한 속도영상화 기법 개발 (Development of Velocity Imaging Method for Motility of Left Ventricle in Gated SPECT)

  • 조미정;이병일;최현주;황해길;최흥국
    • 한국멀티미디어학회논문지
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    • 제9권7호
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    • pp.808-817
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    • 2006
  • 도플러효과를 이용하여 심초음파에서 제공하는 심장의 운동성분석에서 속도지표가 중요하게 사용되고 있지만 판독자 의존도가 높다. 객관성 및 재현성이 높은 게이트 심근 SPECT는 정량화 정보를 제공하고 있으나 속도를 영상화하지는 못하고 있다. 이 연구에서는 게이트 심근 SPECT 영상을 이용하여 속도 영상화 기법을 개발하여 국소 심근 벽 운동에 대한 평가 지표로 사용하고자 하였다. 심근의 분할을 통해 획득되어진 좌표값을 이용하여 심근 모(母)델을 3차원으로 가시화하고 심근벽의 슬라이스별 각 포인트의 속도를 색채 사상을 통해 가시화 하였다. 속도 영상의 유의성 검증을 위하여 총 22명의 데이터에 적용하였으며 정상인 7명과 심근경색 환자 15명으로 그룹을 나누어 각각 적용시킨 결과 정상인의 전체 평균 이동거리는 4.3mm이였고 평균 속도는 11.9mm/s이었으며 환자군에서는 평균 이동거리와 평균 속도가 각각 3.9mm, 10.5mm/s로 나타났으며 정상인의 심벽 움직임 속도가 환자에 비해 빠른 결과를 보였다. 이 논문에서는 게이트 심근 SPECT에서 새로운 지표인 속도 영상화 기법을 개발함으로써 기존의 데이터의 생산성을 향상시키고 재사용성을 증대시켰을 뿐만 아니라 새로운 지표를 이용한 분석을 임상에 적용하는 방안을 제안함으로써 유용성을 검토하였다.

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두정엽 및 후두엽 간질에 대한 수술전략 (Surgical Strategy of Epilepsy Arising from Parietal and Occipital Lobes)

  • 심병수;최하영
    • Journal of Korean Neurosurgical Society
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    • 제29권2호
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    • pp.222-230
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    • 2000
  • Purpose : Resection of the epileptogenic zone in the parietal and occipital lobes may be relevant although only few studies have been reported. Methods : Eight patients with parietal epilepsy and nine patients with occipital epilepsy were included for this study. Preoperatively, all had video-EEG monitoring with extracranial electrodes, MRI, 3D-surface rendering of MRI using Allegro(ISG Technologies Inc., Toronto, Canada), and PET scans. Sixteen patients underwent invasive recording with subdural grid. Eight had parietal resection including the sensory cortex in two. Seven had partial occipital resection. Two underwent total unilateral occipital lobectomy. The extent of the resection was made based mainly on the data of invasive EEG recordings, MRI, and 3D-surface rendering of MRI, not on the intraoperative electrocorticographic findings as usually done. During resection, electrocortical stimulation was performed on the motor cortex and speech area. Results : Out of eight patients with parietal epilepsy, three had sensory aura, two had gustatory aura, and two had visual aura. Six of nine patients with occipital epilepsy had visual auras. All had complex partial seizures with lateralizing signs in 15 patients. Four had quadrantopsia. One had mild right hemiparesis. Abnormality in MRI was noticed in six out of eight parietal epilepsy and in eight out of nine occipital epilepsy. 3D-surface rendering of MRI visualized volumetric abnormality with geometric spatial relationships adjacent to the normal brain, in all of parietal and occipital epilepsy. Surface EEG recording was not reliable in localizing the epileptogenic zone in any patient. The subdural grid electrodes can be implanted on the core of the structural abnormality in 3D-reconstructed brain. Ictal onset zone was localized accurately by subdural grid EEGs in 16 patients. Motor cortex in nine and sensory speech area in two were identified by electrocortical stimulation. Histopathologic findings revealed cortical dysplasia in 10 patients ; tuberous sclerosis was combined in two, hamartoma and ganglioglioma in one each, and subpial gliosis in six. Eleven patients were seizure free at follow-up of 6 months to 37 months(mean 19.7 months) after surgery. Seizures recurred in two and were unchanged in one. Six produced transient sensory loss and one developed hemiparesis and tactile agnosia. One revealed transient apraxia. Two patients with preoperative quadrantopsia developed homonymous hemianopsia. Conclusion : This study suggests that surgical treatment was relevant in parietal and occipital epilepsies with good surgical outcome, without significant neurologic sequelae. Neuroimaging studies including conventional MRI, 3Dsurface rendering of MRI were necessary in identifying the epileptogenic zone. In particular, 3D-surface rendering of MRI was very helpful in presuming the epileptogenic zone in patients with unidentifiable lesion in the conventional MRI, in planning surgical approach to lesions, and also in making a decision of the extent of the epileptogenic zone in patients with identifiable lesion in conventional MRI. Invasive EEG recording with the subdural grid electrodes helped to confirm a core of the epileptogenic zone which was revealed in 3D-surface rendered brain.

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통계적 기법을 이용한 광주지역 민방위비상급수용 지하수 수질 오염도 분석 및 시각화 연구 (Visualization and contamination analysis for groundwater quality of CDEWSF in Gwangju area using statistical method)

  • 장서은;이대행;김종민;김하람;정숙경;배석진;조영관
    • 분석과학
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    • 제31권3호
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    • pp.122-133
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    • 2018
  • 본 연구에서는 광주지역 민방위비상급수시설 101 개소를 대상으로 2006년부터 2016년까지 11년 동안 측정한 지하수 수질자료를 통계적으로 분석하였으며, 각 시설별로 먹는물 수질기준 초과횟수와 평균농도 분석 및 경향성 분석을 실시하고, 그 분석결과를 이용하여 4 개의 오염등급으로 구분하였다. 또한 이를 바탕으로 토지이용 현황별로 각 항목의 지하수 오염도를 평가하였다. 통계적으로 유의한 민방위 비상급수 15 개 항목에 대한 수질기준초과횟수, 평균농도분석, 경향성분석의 세가지 오염지시인자를 분석하여 합산한 결과 Turbidity (51.5 %) > Color (32.7 %) > Nitrate nitrogen (28.7 %) > Hardness (25.7 %) 순으로 오염 지시율이 상대적으로 높게 평가되었다. 오염등급 분석결과 안전(0)부터 오염심각(3)까지 오염등급이 다양하게 분포한 Turbidity, Color, Nitrate nitrogen의 3 개 항목을 제외하면 12 개 항목의 수질 오염등급은 전체의 25 %는 '오염가능한 등급(1)'으로, 75%는 '안전한 등급(0)'으로 평가되었다. QGIS를 이용하여 오염 지시율이 높게 평가된 4 개 항목(Turbidity, Color, Nitrate nitrogen, Hardness)은 오염지도에 작성하여 오염등급을 시각화하였다.

디지털 사진 이미지의 존재론에 관한 연구 -들뢰즈와 보드리야르의 시뮬라크르 개념을 중심으로 (A Study on Ontology of Digital Photo Image Focused on a Simulacre Concept of Deleuze & Baudrillard)

  • 권오상
    • 만화애니메이션 연구
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    • 통권51호
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    • pp.391-411
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    • 2018
  • 본 논문은 질 들뢰즈와 장 보드리야르의 시뮬라크르 개념을 바탕으로 디지털 사진 이미지의 존재론에 관해 살펴보고자 하는 데 그 목적이 있다. 전통적으로 아날로그 사진 이미지는 원본 대상과의 유사성으로 재현의 논리를 따른다. 따라서 아날로그 사진 이미지의 시각적 현실은 주관적 시각에서 조명되고 해석되며 묘사될 수 있지만 해석된 그 현실을 벗어나지는 못한다. 그러나 디지털 사진 이미지는 물질적으로는 존재하지 않고 디지털 알고리즘인 수학적 데이터로 구성된 정보로 존재하게 된다. 이러한 디지털 사진 이미지는 모든 재현에 나타나는 현재성인, 즉 '한때 거기 존재했었음'이라는 피사체의 본질이 이제는 존재하지 않는 것으로 외부의 대상을 지시하거나 재현하지 않는다. 따라서 디지털 이미지는 유사성이 존재하지 않고 더 이상 지표적 지시 능력을 지니지 않는다. 이러한 디지털 사진 이미지는 가상의 영역으로 전환되었다는 것을 의미하는데, 이는 이미 존재하는 것의 재현이 아닌, 아직 존재하지 않는 것의 현시라 할 수 있다. 이러한 디지털 사진 이미지의 비실재성은 현실과 실재 그리고 가상에 대한 이해를 변화시킨다. 이제는 무엇이 실재이고 가상인지 구분하는 것 자체가 무의미하게 되고, 이는 기술의 발달로 디지털 이미지가 발생한 것이 아닌, 기존의 이미지와는 근본적으로 완전히 다른 새로운 이미지라는 것이다. 결국, 오늘날의 디지털 이미지는 존재했으니 대상을 가시화하는 단계를 넘어, 이제는 존재하지 않았던 것들이 가시화되고 있으며, 현실도 가상으로 작동한다는 것이다. 이는 디지털 이미지가 우리 현실을 재현한 것이 아니라 다른 현실을 사실적으로 재현한 것이라 할 수 있다. 즉 대상과 아무런 관련이 없는 이미지 생성을 하는 가상적 재현, 즉 시뮬라크르라 할 수 있다. 가상으로서의 시뮬레이션 된 세계에서 현실은 무한한 가능성을 가질 수 있고 그것은 과거나 현재의 모습이 아닌 고정되지 않고 무한히 변화할 수 있는 아직 현실화되지 않은 무한한 잠재태로서의 가능성을 가진다.