• 한국수산과학회지
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• 제32권4호
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• pp.458-465
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• 1999

Distribution of the proteolytic activities of crude pretense extracted from the viscera of ten kinds of fish was examined. Their proteolytic activities on proteinous substrates (azocasein, hemoglobin, and casein) from the viscera of anchovy, bastard flatfish, mackerel and red sea bream were higher than those of other fishes, and the crude pretenses were further fractoinated with acetone or ammonium sulfate. Optimum concentrations for pretenses fractionation were $0\~55\%$ for acetone and $30\~70\%$ for ammonium sulfate. The fractionated viscera pretense of mackerel showed the highest proteolytic activity among four kinds of fishes. Activities of cathepsin D- and pepsin-like enzymes at pH 3.0, cathepsin L-, B-, H- and G-like enzyme at pH 6,0, and Hypsin- and chymotrypsin- like enzymes (pH 8.0) were detected in the fractionated viscera pretense, whereas activities of cathepsin L- and chymoeypsin-like enzyme were observed in commercial pretenses. Proteolytic activities of Alcalase, Protamex, and Aroase AP-10 for azocasein were slightly higher than the fractionated viscera pretenses, but their amidolytic activities at pH 6.0 and 8.0 toward synthetic substrates were lower than counterpart. The fractionated pretenses from fish viscera would be utilized as commercial pretenses.

• 대한한의학원전학회지
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• 제30권1호
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• pp.167-184
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• 2017

Objectives : The paper attempted to analyze the contents found in the networks of five viscera in Yixuerumen's Zangfutiaofen published by Li Chan in 1575, and tried to reveal the body structure dealt in the contents through anatomical verification. Methods : Books such as Huataxianshengneizhaotu, Tushupian, and Shisijingfahui were used to compare, understand, and interpret the original texts and annotations of Yixuerumen in an attempt to verify these through anatomy reference texts and computer programs. Results : In Yixuerumen, some contents of the networks of five viscera were revised to see feixi as not one but two, and explained the relationship of intestinal organs through heart and lung. Anatomically speaking, the networks of five viscera has a vertical structure that unfolds top to bottom centering on aorta, and has many organs connected to it such as trachea, esophagus, splenic artery, pulmonary trunk, ureter, mesentery arteriovenous, gonadal artery, and ductus deferens. Conclusions : The networks of five viscera has a great significance in that it is the fruit of much efforts where oriental medicine tried to explain not only the functions of five viscera, but also the creation and circulation of qi, blood, and bodily fluids through anatomical observation.

• 동의생리병리학회지
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• 제33권6호
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• pp.322-333
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• 2019

The purpose of this thesis is to show the historical context of tongue-viscera correspondence scheme based on as wide investigation as possible. For this purpose, we collected and investigated 35 books related to tongue diagnosis. As a result, we faound the following: There were some tentative trials to associate viscera or viscera meridians with tongue areas since shown in the Effective formulae of inherited medical works (世醫得效方, 1337). The main stream of tongue-viscera correspondence scheme was the form in which the apex, the center, and the root of tongue are associated with Heart, Spleen (and Stomach), and Kidney. On the viscera correspondence to the sides of tongue, there had been two streams. The first one is originated from the Upper-most Book on Shanghan (傷寒第一書, 1780), in which the sides of tongue are assumed to be associated with Liver and Gall-bladder. The second one is originated from the Ikeda Family's Chant on the Tongue (池田家舌函口訣, 1807), in which the left side and the right side of tongue are assumed to be associated with Liver and Lung separately. The former type have been accepted as the standard form in modern traditional Asian medicine education. In addition to the above types, three other correspondence schemes were also existed, but have disappeared now. These days, some new correspondence schemes are being suggested based on new approaches.

• 대한한의학원전학회지
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• 제25권4호
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• pp.123-147
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• 2012

Objective : By establishing the Five Viscera Source Point Acupuncture as the targeted acupuncture treatment for stadardization, as the first step, this study was conducted to sort the indications of each acupuncture remedies, which can be referred as one of the most important factors in acupuncture treatment, based on Yeoungchu. Method : This study selected only the contents related to indications of five viscera, by extracting the relevant sentences from Yeoungchu using the search words Liver(Liver Meridian, First Yin), Heart(Pericardium, Heart Meridian, Second Yin), Spleen(Spleen meridian, Third Yin), Lung(Lung Meridian, Third Yin), and Kidney(Kidney Meridian, Second Yin). Result & Conclusion : 1. We selected and extracted text related to liver disease from Chapter 16, heart (pericardium) disease from Chapter 16, spleen disease from Chapter 19, lung disease from Chapter 17, and finally kidney disease from Chapter 17 of Yeoungchu. 2. The basic theory of applying Five Viscera Source Point Acupuncture to five viscera diseases is first assorting the diseases according to its state (i.e. deficiency or excess), then draining the source point of the appropriate viscus in case of excess, or supplementing the source point of the appropriate viscus in case of deficiency. 3. For the correct application of Five Viscera Source Point Acupuncture, the classification of the disease, not only the judgement on its state, must be presented systematically and synthetically in combination with Four Examinations. Therefore the follow-up studies needs to be conducted.

• 한국식품영양과학회지
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• 제21권2호
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• pp.175-180
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• 1992

말쥐치 가공 중 폐기되고 있는 말쥐치 내장중의 지질을 보다 효율적으로 이용하기 위해 탈검, 탈산, 탈색 및 탈취 등 말쥐치 내장유의 정제조건에 대하여 검토하였다. 말쥐치 내장유 100$m\ell$에 대하여 4% 옥살산을 20$m\ell$ 첨가하고 이어서 6$0^{\circ}C$에서 15분간 교반하여 탈검한 다음 이것에 4M의 알칼리 용액을 계산량 보다 0.5% 과잉으로 첨가한 후 6$0^{\circ}C$에서 30분간 탈산 처리하는 것이 효과적이었다. 탈산처리한 말쥐치 내장유의 10%에 해당하는 산성 백토를 첨가하고 감압하에 6$0^{\circ}C$에서 20분간 탈색 처리한 후 압력을 4 torr 이하로 유지하면서 수증기 증류법으로 18$0^{\circ}C$ 에서 60분간 처리한 말쥐치 내장유가 물리, 화학적 성상이 가장 우수하였다.

• 한국식품과학회지
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• 제27권2호
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• pp.141-146
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• 1995

본 연구는 영양적으로 양호하고 가격면에서도 저렴한 단백질 자원을 개발하기 위하여 가다랭이 내장을 에틸 알코올 및 단백질 가수분해 효소인 펩신으로 처리함으로써 식품소재로서의 이용가능성을 검토한 것이다. 이 가다랭이 내장 단백질 가수분해물의 성분과 가수분해도 및 용해도 등의 변화를 조사하고, 모조간장 또는 식빵 제조시 첨가하여 관능검사를 통하여 영양적 가치와 기호성 등을 확인하였던 바, 가다랭이 내장으로부터의 단백질 농축물은 40% 정도의 용해도를 나타내었으나, 단백질 가수분해물은 90% 정도까지 향상되었다. 한편 필수아미노산 함량면에서 단백질 가수분해물의 경우 전체 아미노산의 63.2%로 우수한 단백질 소재임을 알 수 있었고, 타우린의 함량이 9.4%로 상당히 높은 특징을 보였다. 또한 내장 단백질 가수분해물을 첨가하여 모조간장 및 식빵을 제조한 다음 관능평가 결과, 모조간장의 경우는 물 100ml에 다른 원료들과 함께 내장 단백질 가수분해물을 10.0g 첨가했을 때 제품의 색깔, 맛, 냄새 등에서 좋은 평점을 얻었으며, 식빵의 경우는 $3{\sim}5%$ 수준의 내장 단백질 가수분해물 첨가시 제품의 맛, 색깔, 냄새, 조직 및 촉감 등의 품질면에서 손색이 없었다.

• Korean Chemical Engineering Research
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• 제48권6호
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• pp.741-746
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• 2010

오징어내장에서 초임계 이산화탄소를 이용한 추출잔류물에 에탄올을 이용하거나 초임계 처리를 하지 않은 오징어내장에서 다양한 유기용매를 이용하여 인지질을 추출하였다. 초임계이산화탄소 추출은 동결 건조된 오징어 내장 시료를 이용 $45^{\circ}C$ 20 MPa 조건에서 수행되었으며 무극성 지질의 추출에 사용되었다. 동결 건조된 오징어 내장은 클로로포름, 헥산, 메탄올, 에탄올을 이용해 인지질 추출을 시행하고 초임계 이산화탄소 추출을 거친 내장 시료는 에탄올 처리하여 인지질을 추출하였다. 각 인지질 추출과정에서 pH는 5.7로 고정하여 시행하였다. 각각의 인지질의 조성을 검출하기 위하여 evaporative light scattering detector(ELSD)를 이용하여 HPLC 분석을 행하였다. 포스파티딜 콜린(PC)과 포스파티딜 이노시톨(PI)은 초임계이산화탄소 잔류물을 에탄올 추출한 것에서 동결건조물을 에탄올만을 사용하여 추출한 것보다 많은 양이 검출되었다. 하지만 포스파티딜 에탄올아민(PE)과 포스파티딕 에시드(PA)의 경우 동결 건조된 오징어내장을 에탄올만을 사용하여 추출한 인지질에서 많은 양이 검출되었다. 초임계 이산화탄소 처리 후 회수된 추잔 물질에 에탄올을 사용하여 인지질 혼합물을 분리 후 분리된 인지질 혼합물에 대해 가스크로마토그래피를 이용하여 지방산을 분석한 결과 docosahexanoic acid(DHA)의 함량이 매우 높은 것을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제10권2호
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• pp.210-214
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• 1997

Ruminal degradation characteristics and intestinal availability of crude protein (CP) in four animal-origin feeds (fish meal, meat meal, viscera meal, feather meal) were estimated by mobile nylon bag technique. Three ruminally and duodenally cannulated Holstein dairy cows (average body wt. 550kg) fed a diet containing 40% concentrate and 60% orchard grass hay on a dry matter (DM) basis. Assuming that the outflow rate of diet in rumen is 5% per hour (k =0.05), contents of quickly degradable CP (QDP), slowly degradable CP (SDP), and undegradable CP (UDP) in the rumen were 27.6%, 9.4%, 63.0% for fish meal, 34.3% 28.1%, 37,6% for meat meal, 43.9%, 12.5%, 43.6% for viscera meal, and 14.4%, 15.8%, 69.8% for feather meal, respectively. Intestinal CP degradability was 51.0% for fish meal, 27.2% for meat meal, 37.9% for viscera meal and 56.2% for feather meal. Available UDP in the intestinal tract was contained 288 g, 217 g, 246 g and 423 g per kilogram DM of diet in fish meal, meat meal, viscera meal and feather meal, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.315-321
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• 2005

Supercritical carbon dioxide extraction was investigated as a method for removing lipids and bad flavor from tuna viscera. To find the optimum conditions, different experimental variables, such as pressure, temperature, flow rate of solvent and sample size, were evaluated for the effective removal of lipids and the undesirable smell. Ethanol was used as the entrainer, with a $3\%$ by vol $CO_2$ flow rate. By increasing the pressure at constant temperature, the efficiency of the lipid removal was improved and the protein was concentrated without denaturalization. The main fatty acids extracted from the tuna viscera were palmitic acid (16:0), heptadecanoic acid (17:1), oleic acid (18:1) and docosahexaenoic acid (22:6). The major amino acids in the tuna viscera treated by supercritical carbon dioxide were glutamic acid, leucine and lysine, and the free amino acids were L-proline, taurine and L-$\alpha$-aminoadipic acid.

• 한국수산과학회지
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• 제41권3호
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• pp.176-181
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• 2008

To evaluate the effective use of endoprotease from squid viscera as a food processing aid, various methods of fractionating endoprotease from viscera of the Argentina shortfin squid (Illex argentinus) were evaluated. The endoprotease-positive fractions of each fractionation were fraction II (30-40%, w/w) with cold acetone, fraction IV (50-60% saturation) with ammonium sulfate, fraction UF with anion exchange chromatography, and fraction II (15-24 kDa) with gel filtration. The specific activities (approximately 25 U/mg) of the fractions using ammonium sulfate and gel filtration were higher than the others. Total azocaseinolytic activity and recovery of the positive fraction using gel filtration were 806.95U and 37.82%, respectively, and were the highest among the positive fractions. Based on the results, gel filtration was the most efficient method for fractionating endoprotease from the viscera of Illex argentinus.