• 제목/요약/키워드: Virulence

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Detection of virulence, specific genes and antibiotic resistance of isolated Salmonella spp. strains from rabbits infected with salmonellosis

  • Huynh Van Chuong;Nguyen Minh Tuan;Nguyen Thi Nhu Anh;Le Thi Lan Phuong;Nguyen Xuan Hoa
    • 대한수의학회지
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    • 제63권2호
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    • pp.16.1-16.6
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    • 2023
  • Salmonella spp. are pathogens involved in most salmonellosis in rabbits. This study examined Salmonella disease in rabbits raised in Thua Thien Hue, Vietnam. Two hundred and 56 rectal swabs of rabbits were taken, and a carrier rate of 33.98% was found. In addition, all the isolated Salmonella spp. strains were 100% motile; positive for H2S, catalase, Voges Proskauer, coagulase, citrate, maltose, and dextrose; and negative for indole, methyl red, urease, oxidase, sucrose, and lactose. The Kirby-Bauer method showed that these Salmonella strains were susceptible to doxycycline (93.2%), tetracycline (84.1%), and levofloxacin (65.9%). On the other hand, they were highly resistant to streptomycin (95.5%), ampicillin (93.2%), colistin (40.9%), and gentamicin (34.1%). Furthermore, polymerase chain reaction used to screen for virulence and specific genes of Salmonella strains showed that all Salmonella strains isolated carried InvA, fimA, and Stn.

The Relationship between the Sugar Preference of Bacterial Pathogens and Virulence on Plants

  • Ismaila Yakubu;Hyun Gi Kong
    • The Plant Pathology Journal
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    • 제39권6호
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    • pp.529-537
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    • 2023
  • Plant pathogenic bacteria colonize plant surfaces and inner tissues to acquire essential nutrients. Nonstructural sugars hold paramount significance among these nutrients, as they serve as pivotal carbon sources for bacterial sustenance. They obtain sugar from their host by diverting nonstructural carbohydrates en route to the sink or enzymatic breakdown of structural carbohydrates within plant tissues. Despite the prevalence of research in this domain, the area of sugar selectivity and preferences exhibited by plant pathogenic bacteria remains inadequately explored. Within this expository framework, our present review endeavors to elucidate the intricate variations characterizing the distribution of simple sugars within diverse plant tissues, thus influencing the virulence dynamics of plant pathogenic bacteria. Subsequently, we illustrate the apparent significance of comprehending the bacterial preference for specific sugars and sugar alcohols, postulating this insight as a promising avenue to deepen our comprehension of bacterial pathogenicity. This enriched understanding, in turn, stands to catalyze the development of more efficacious strategies for the mitigation of plant diseases instigated by bacterial pathogens.

Raffaelea quercus-mongolicae와 Raffaelea spp. 인공접종에 의한 신갈나무 줄기에서의 병원성 평가 (Assessment of Virulence of Raffaelea quercus-mongolicae and Raffaelea spp. Isolates by Artificial Inoculation of Quercus mongolica Logs)

  • 손수연;서상태;박형철
    • 식물병연구
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    • 제20권4호
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    • pp.270-274
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    • 2014
  • 본 연구의 목적은 신갈나무에서 참나무시들음병 병원균인 Raffaelea quercus-mongolicae의 지역별 균주와 기타 Raffaelea 속 근연종의 병원성을 평가하는 것이다. 이 연구에서는 신갈나무에 다양한 균주를 접종한 후, 변재부 내 변색정도로 각 균주의 병원성을 판단하였다. 그 결과 변색부의 평균 측면 확산 정도는 국내에서 분리한 Raffaelea quercus-mongolicae 병원균(8.69 cm)이 일본 병원균(7.51 cm)이나 기타 Raffaelea 속 근연종(3.35 cm)의 평균보다 높았고, 국내 지역별 Raffaelea quercusmongolicae 균주간에는 변색부 측면 확산정도가 4.71-14.90 cm로 병원성의 차이를 보였다. 균주간의 변색부 면적 확산정도는 $1.57-8.42cm^2$로 서로 다른 병원성의 차이를 보였으며, 가장 높은 병원성은 강원도에서 분리한 YY와 전라북도에서 분리한 wj43에서 나타났다.

소아 요로감염의 원인 Escherichia coli 균의 계통 분류와 독성인자 분석 (Phylogenetic Groups and Virulence Factors of Escherichia coli Causing Urinary Tract Infection in Children)

  • 김지목;조은영;이재호
    • Pediatric Infection and Vaccine
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    • 제22권3호
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    • pp.194-200
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    • 2015
  • 목적: 요로감염은 소아에서 흔한 세균 감염이며, Escherichia coli가 주요 원인균이다. 본 연구는 우리나라에서 소아 요로감염을 일으키는 E. coli의 계통 분류와 독성인자를 분석하고자 하였다. 방법: 2010년 10월부터 2013년 4월까지 요로감염으로 입원한 33명의 소아 환자로부터 검출된 E. coli균주를 대상으로 하였다. 중합효소연쇄반응을 통해 E. coli의 계통 분류 및 5가지 독성인자(fimH, sfa, papA, hylA, and cnf1)를 조사하였다. E. coli의 분자유전학적 특징을 환자의 임상적 진단과 동반된 방광요관 역류에 따라 분석하였다. 결과: 대부분의 요로병원성 E. coli 는 계통 분류에서 B2군(84.8%)에 속했으며, 나머지는 모두 D군(15.2%)에 해당되었다. 독성인자는 fimH (100%), sfa (100%), hylA (63.6%), cnfI (63.6%), 그리고 papA (36.4%)의 분포를 보였다. 임상 진단에 따른 계통 분류에서 급성 신우신염의 경우 B2군이 92.3%, D군이 7.7%를 나타냈으며, 방광염에서는 B2군에서 57.1%, D2군은 42.9%였다. 독성인자는 양 군에서 비슷하게 분포하였다. 급성 신우신염에서 방광요관 역류의 유무에 따른 계통 분류의 분포에는 차이가 없었으나, 독성인자의 경우 papA 유전자가 방광요관 역류가 동반되지 않은 군에서보다 방광요관 역류 군에서 적게 나타났다(43.8% vs. 20.0%, P=0.399). 결론: 본 연구는 국내 소아 요로감염의 원인 E. coli 균주의 분자유전학적 역학 자료를 제시하였으며, 이 결과는 향후 소아 요로감염의 발생 기전을 이해하는 데 기초가 될 것으로 생각된다.

Analysis of whole genome sequencing and virulence factors of Vibrio vulnificus 1908-10 isolated from sea water at Gadeok island coast

  • Hee-kyung Oh;Nameun Kim;Do-Hyung Kim;Hye-Young Shin;Eun-Woo Lee;Sung-Hwan Eom;Young-Mog Kim
    • Fisheries and Aquatic Sciences
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    • 제26권9호
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    • pp.558-568
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    • 2023
  • Vibrio vulnificus is an aquatic bacterium causing septicemia and wound infection in humans. To understand this pathogen at the genomic level, it was performed whole genome sequencing of a cefoxitin-resistant strain, V. vulnificus 1908-10 possessing virulence-related genes (vvhA, viuB, and vcgC) isolated from Gadeok island coastal seawater in South Korea. The genome of V. vulnificus 1908-10 consisted of two circular contigs and no plasmid. The total genome size was estimated to be 5,018,425 bp with a guanine-cytosine (GC) content of 46.9%. We found 119 tRNA and 34 rRNA genes respectively in the genome, along with 4,352 predicted protein sequences. Virulence factor (VF) analysis further revealed that V. vulnificus 1908-10 possess various virulence genes in classes of adherence, antiphagocytosis, chemotaxis and motility, iron uptake, quorum sensing, secretion system, and toxin. In the comparison of the presence/absence of virulence genes, V. vulnificus 1908-10 had fur, hlyU, luxS, ompU, pilA, pilF, rtxA, rtxC, and vvhA. Of the 30 V. vulnificus comparative strains, 80% of the C-genotype strains have all of these genes, whereas 40% of the E-genotype strains have all of them. In particular, pilA were identified in 80% of the C-type strains and 40% of the E-type strains, showing more difference than other genes. Therefore, V. vulnificus 1908-10 had similar VF characteristics to those of type C strains. Multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin of V. vulnificus 1908-10 contained 8 A-type repeats (GXXGXXXXXG), 25 B.1-type repeats (TXVGXGXX), 18 B2-type repeats (GGXGXDXXX), and 7 C-type repeats (GGXGXDXXX). The National Center for Biotechnology Information (NCBI) Basic Local Alignment Search Tool (BLAST) showed that the RtxA protein of V. vulnificus 1908-10 had the effector domain in the order of cross-liking domain (ACD)-C58_PaToxP-like domain- α/β hydrolase-C58_PaToxP-like domain.

인삼근부병에 관한 연구 1 (On the Root Rot of Ginseng(I))

  • 김종희;이민웅
    • 미생물학회지
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    • 제12권2호
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    • pp.94-98
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    • 1974
  • This study was conducted from April 1 to September 31, 1973. Ten strains of Fusarium spp. were isolated from the diseased ginseng in two local areas at Kangwha-Gun nad Kumsan-Gun in Korea. Among of them, 2 strains ($G_1$, $G_4$) did not have virulence to ginseng in reinoculation. Their cultural, morphological characteristic and hose virulence to pea seedling were examined. Taxonomical identification of 8 isolates followed by the method of Wollenweber, Snyder nad Toussoun, Booth, Matuo and Snyder. All of eight strains were identified as the Fusarium solani f.sp.pisi(Jones) Synd. et Hans.

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Mutation of Virulence Candidate Genes of Ralstonia solanacearum

  • Park, Eun-Jin;Lee, Seung-Don;Um, Hae-Young;Chung, Eu-Jin;Lee, Myung-Hwan;Moon, Byung-Ju;Lee, Seon-Woo
    • 한국생명과학회:학술대회논문집
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    • 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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    • pp.104.1-104.1
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    • 2007
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Cloning of phosphoglucomutase gene (pgm) in Streptococcus parauberis

  • Woo, Sung-Ho;Han, Hyun-Ja;Kim, Do-Hyung;Park, Soo-Il
    • 한국어병학회지
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    • 제23권2호
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    • pp.239-244
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    • 2010
  • Here, we have cloned and sequenced Streptococcus parauberis pgm gene, encoding the enzyme phosphoglucomutase (PGM), which is known to be in association with virulence in other streptococcal species. The PGM of S. parauberis is the most closely related to that of S. iniae based on their amino acid sequences.

Quorum Sensing Regulation of Biofilm Formation by Periodontal Pathogens

  • Choi, Bong-Kyu
    • International Journal of Oral Biology
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    • 제43권4호
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    • pp.171-175
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    • 2018
  • Quorum sensing (QS) is a cell density-dependent communication mechanism between bacteria through small signaling molecules. When the number of QS signaling molecules reaches a threshold, they are transported back into the cells or recognized by membrane-bound receptors, triggering gene expression which affects various phenotypes including bioluminescence, virulence, adhesion, and biofilm formation. These phenotypes are beneficial for bacterial survival in harsh environments. This review summarizes the application of QS inhibitors for control of biofilm formation and virulence expression of periodontal pathogens.

벼 흰빛잎마름병균의 병원성 발현과 벼의 품종 및 생육시기와의 관계 (Interactions among Isolates of Xanthomonas oryzae, Rice Varieties and Growth Stages of Hosts)

  • 조용섭;이순구
    • 한국응용곤충학회지
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    • 제18권2호
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    • pp.77-84
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    • 1979
  • 벼 흰빛잎마름병균의 10균주(I군 3균주, II군 4균주, III군 3균주)를 저항성이 서로 다른 벼품종 '밀양 23호'(김남풍군), '유신'(황옥군), '통일'(Rantaiemas군)의 유묘기와 분얼초기, 최고분얼기, 지엽기에 각각 접종하였다. 그 결과 1) 병원성 발현의 양상이 균주 간 매우 다양하였다. 2) 특히 II군의 균주들은 같은 군균내에서도 변이가 매우 큰 것으로 나타났다. II군은 더 많은 균형으로 세분될 수 있었다. 3) 생육시기 별로 병원성 발현 양상이 달랐으며, 대체로 지엽기 이후 높은 발병을 나타내었다. II군중 G 7716균주는 출수이후에야 비로소 '유신'품종에 발병을 일으킬 수 있었다. 4) 통계분산분석(ANOVA) 결과, 각각 다른 균형의 균주, 품종, 생육시기가 발병정도의 분산에 주요인으로 작용하였고, 균주와 품종간의 상호작용(interaction)에 유의성이 있었으나 다른 요인간에는 없었다.

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