• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2016년도 학술발표회
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• pp.200-200
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• 2016

Climate changes have impacted to many sectors including water resources in Vietnam. Vietnam is agricultural development country having a lot of reservoirs. These reservoirs play a very important role in flow regulation for water supply to economic sectors. In the context of undesirable impacts of climate change such as increasing temparature, evaporation, changing rainfall and rainfall pattern, water demands and inflow to reservoirs also are being influenced. This leads to changes of resevoir exploitation effects that needs to be assessed for adaptation solutions. This arcticle summaries the development of a tool for quick assessement of climate change impacts on exploitation effect of reservoir in central provinces of Vietnam.

• IMMUNE NETWORK
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• 제12권1호
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• pp.33-39
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• 2012

Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc) and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59 was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.

• 대한치과기공학회지
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• 제31권2호
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• pp.45-52
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• 2009

Esthetic restorations have been widely used in dental practice, although many studies have focused on the development and improvement of all ceramic restorations. The success of esthetic restorations depends primarily on an optimal bond strength between various veneered ceramics and core materials for esthetic restorations. The purpose of this study was to compare the shear bond strength between various veneered ceramics and core materials for esthetic restorations. 30 metal cores and 20 zirconia cores were fabricated and divided into five groups according to veneered ceramic materials such as Creation porcelain powder, Cercon Ceram Kiss, and IPS e.max ZirPress. Thirty spacimens were prepared using Creation porcelain powder, veneered 3mm height and 3mm in diameter, over the metal cores (n=10). Twenty specimens were prepared using Cercon Ceram Kiss and Zirpress, veneered 3mm height and 3mm in diameter, over the zirconia cores (n=10). The shear bond strength test was performed in a universal testing machine with a crosshead speed of 1mm/min. Ultimate shear bond strength data were analyzed with One-way ANOVA and the Scheffe's test (p=.05). Within the limits of this study, the following conclusions were drawn: The mean shear bond strengths (MPa) were: 18.44 for Uni metal VH/Creation (NCUC); 18.72 for Heraenium/Creation (NCHC); 16.23 for Wirobond C/Creation (NCWC); 13.88 for Zirconia core/$110{\mu}m$ $Al_2O_3$ sandblasting/Cercon Ceram Kiss (ZS110P); 14.61 for Zirconia core/No surface treatment/IPS e.max ZirPress (ZNTH). The mean shear bond strength for NCUC (Uni metal VH/Creation), NCHC (Heraenium/Creation) and NCWC (Wirobond C/Creation) were significantly superior to ZS110P (Zirconia core/$110{\mu}m$ $Al_2O_3$ sandblasting/Cercon Ceram Kiss) and ZNTH(Zirconia core/No surface treatment/IPS e.max ZirPress) (p<0.05).

• 한국가축번식학회지
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• 제18권1호
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• pp.39-46
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• 1994

This experiment was carried out to develop an in vitro culture system for rabbit embryos. The zygotes or 2-cell embryos were collected from the oviducts of the superovulated and mated does with D-PBS/10% FCS at 24 hours after hCG injection. The in vitro developmental rate of blastocyst formation and the number of nuclei in the embryos were examined under the following treatments; 1) TCM-199 with 10% FCS, 2) EBSS with 10% FCS, 3) rabbit vitreous humor(VH), 4) TCM-199 with 10% FCS＋BOEC, 5) TCM-199 with 10% FCS＋ROEC, 6) EBSS with 10% FCS＋BOEC and 7) EBSS with 10% FCS＋ROEC. For a comparative study of in vivo and in vitro development, the fresh blastocysts, which were developed in vivo for 96 hours after hCG injection, were collected from the uterus and their numbers of nuclei were counted. 1. The zygotes or 2-cell embryos developed to the blastocyst stage in TCM-199, EBSS and VH at the rates of 93, 92 and 89%, respectively. 2. The higher developmental rates 95~98% of blastocyst formation was achieved when the embryos were co-cultured with a monolayer of bovine or rabbit oviductal epithelial cells in TCM-199 or EBSS. No significant difference in developmental rates was shown between bovine and rabbit oviductal epithelial cells. 3. In a comparative study of in vivo and in vitro development, the total numbers of nuclei were significantly less in the in vitro cultured embryos(104~224) than the in vivo developed embryos(1, 0090 at 96 hours after hCG injectin. 4. The mean cell cycle numbers in the embryos cultured for 72 hours in TCM-199 with 10% FCS, EBSS with 10% FCS, TCM-199 with 10% FCS＋BOEC, TCM-199 with 10% FCS＋ROEC, EBSS with 10% FCS＋BOEC and in vivo was 7.38, 6.63, 7.76, 7.69, 7.01 and 9.92, respectively. From these results, it can be suggested the optimal culture system for in vitro culture of rabbit embryos is a co-culture system with bovine or rabbit oviductal epithelial cells in TCM-199 with 10% FCS. Considering the significant reduction in total numbers of nuclei in the in vitro cultured embryos, the advanced research on development of in vitro culture system for rabbit embryos is expected.

• 한국전자파학회논문지
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• 제10권2호
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• pp.302-311
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• 1999

본 논문에서는 실내 무선환경에서 최적 편파 다이버시티를 구성하는 방안을 제시하기 위하여 먼저 이론적 해석을 통하여 교차편파식별도(XPD : Cross Polarization Discrimination)를 해석하고 이를 실제 측정 데이터와 비교하였다. 그 결과 아직까지 본 연구실에서 가장 좋은 편파 다이버시티로 확인된 바 있는 원형편파안테나 로 송신하고 수직, 수평편파안테나로 수신하는 경우(CV-CH)의 교차편파식별도(XPD)는 수직편파안테나로 송신하고 수직, 수평편파안테나로 수신하는 경우(VV-VH)와 수평면파안테나로 송신하고 수평편파, 수직편파 안테나로 수신하는 경우(HH-HV)보다 우수함을 확인하였다. 본고에서는 더 좋은 CV -CH 편파다이버시티 구 성을 위해 수신단에서 교차편파식별도(XPD) 값만큼 보상하여 누적확률분포도를 통한 다이버시티 효과를 평 가하였다. 그 결과 보상을 하여 교차편파식별도(XPD) 값을 낮게 한 경우가 보상하지 않은 경우보다 상당한 다이버시티 효과가 있음을 확인하였다. 한편, 송신측의 원형편파 안테나의 축 비를 교정하여 타원편파 안테나 로 구성하고, 수신측에서 수평 및 수직 안테나로 수신하면 신호간의 교차편파식별도(XPD)가 최소화되어 편파다이버시티 효과를 향상시킬 수 있음을 알 수 있었다.

• Journal of Animal Science and Technology
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• 제60권1호
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• pp.29.1-29.6
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• 2018

Background: Dietary supplementation of zinc oxide (ZnO) to 2000 to 4000 mg/kg is known to be effective for the prevention and treatment of post-weaning diarrhea in the pig. Such a 'pharmacological' supplementation, however, can potentially result in environmental pollution of the heavy metal, because dietary ZnO is mostly excreted unabsorbed. Two experiments (Exp.) were performed in the present study to determine the effects of a lipid-coated ZnO supplement Shield Zn (SZ) compared with those of ZnO. Methods: In Exp. 1, a total of 240 21-day-old weanling pigs were fed a diet supplemented with 100 mg Zn/kg as ZnO (ZnO-100), ZnO-2500, SZ-100, or SZ-200 in 24 pens for 14 days on a farm with its post-weaning pigs exhibiting a low incidence of diarrhea. Exp. 2 was performed using 192 24-day-old piglets as in Exp. 1 on a different farm, which exhibited a high incidence of diarrhea. Results: In Exp. 1, fecal consistency (diarrhea) score (FCS) was less for the ZnO-2500 and SZ-200 groups than for the SZ-100 group (P < 0.05), with no difference between the SZ-100 and ZnO-100 groups. Both average daily gain (ADG) and gain:feed ratio were less for the SZ-200 group than for the ZnO-2500 group, with no difference between the ZnO-100 group and SZ-100 or SZ-200 group. The villus height (VH), crypt depth (CD), and VH:CD ratio of the intestinal mucosa were not influenced by the treatment. In Exp. 2, FCS was lowest for the ZnO-2500 group, with no difference among the other groups. However, neither the ADG nor gain:feed ratio was influenced by the treatment. Conclusion: Results suggest that physiological SZ supplementation has less beneficial effects than pharmacological ZnO for the alleviation of diarrhea irrespective of its severity and for promoting growth without influencing their integrity of the intestinal mucosal structures with little advantage over physiological ZnO in weanling pigs with a small pen size.

• 대한원격탐사학회지
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• 제26권2호
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• pp.123-131
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• 2010

이 논문에서는 지상용 SAR (GB-SAR) 시스템의 간섭기법을 이용하여 특정 산란체의 인위적 변위를 탐지하고 대기습도보정을 통하여 정확도를 향상시켰다. GB-SAR 자료는 중심주파수 5.3 GHz, 거리 해상도 25 cm, 방위해상도 $0.324^{\circ}$로 모든 편파(HH, VV, VH, HV)에 대해 얻어졌다. 삼각삼면반사체(triangular trihedral corner reflector)를 시스템 전방 160 m 지점에 위치시킨 후 인위적으로 0 - 40 mm의 변위를 주어 측정하였다. 그 결과, 모든 편파에서 실제 변위와 GB-SAR 시스템을 통한 측정 변위의 RMS 오차는 1.22 mm로 나타났으며, 실제변위 40 mm 일 때의 최대 측정오차는 HH편파에서 2.72 mm로 나타났다. 대기 중 습도에 대한 보정을 실시하였고 그 결과, RMS 오차는 0.52 mm로 줄어들었다. 이를 통해 GB-SAR 시스템은 밀리미터 이하의 정밀도가 요구되는 자연산란체나 인공구조물의 변위측정 및 안정성 평가 분야에 적용이 가능할 것으로 판단된다.

• 대한원격탐사학회지
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• 제23권4호
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• pp.257-272
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• 2007

이 논문에서는 촬영 시기 및 촬영 모드(주파수, 편파, 입사각)에 있어서 여러 가지 조건을 가지는 다양한 SAR 자료로부터 특징을 추출하여 토지 피복 항목과의 상호 연관성을 분석하였다. 현재까지 가용한 인공위성 SAR 영상의 촬영 조건을 고려하여 다음의 두 가지 경우로 구분하여 특징을 추출하였다. 첫째, 단일 모드로 다중 시기에 얻어진 SAR 자료로부터 긴밀도, 시간적 변이도, 주성분 변환에 의한 특징들을 추출하였다. C-밴드인 ERS-1/2, ENVISAT SAR, Radarsat-1 자료와 L-밴드인 JERS-1 SAR 자료를 대상으로 이러한 특징들을 각각 추출하였으며, 일반적인 토지 피복 항목과의 연관성 분석을 통해 다중 센서의 특성 차이를 비교 분석하였다. 여러 특징들 중에서 Tandem 긴밀도는 대체적으로 토지 피복 항목간 구별력이 가장 좋게 나타났다. C-밴드 SAR 자료의 장기간 긴밀도에서는 도심 지역의 구분이 용이하였으며, 시간적 변이도에서는 모든 센서 자료에서 논 지역이 가장 높은 값을 나타내었다. 또한 시계열 후방 산란 계수와 긴밀도의 주성분 변환에 기반한 특징들에서는 토지 피복과 관련된 부가 정보 추출이 가능하였다. 둘째, 다중모드(편파, 입사각)로 비슷한 시기에 얻어진 SAR 자료로부터 편파비와 다중 채널 변이도를 주요 특징으로 추출하여 토지 피복 항목별로 비교하였다. 그 결과, VH/VV 편파비로부터 산림과 밭 항목의 구분력이 향상되는 것으로 나타났다. 이 연구의 분석 결과는 향후 다양한 모드의 시계열적 SAR 자료 및 지상 산란계 실험을 통한 다양한 사례 연구 결과와 결합된다면, SAR 자료를 이용한 토지 피복 분류의 정확도 향상을 위한 기초 정보로 활용될 수 있을 것으로 기대된다.

• 한국지구과학회지
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• 제40권5호
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• pp.502-517
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• 2019

지구온난화와 급속한 기후 변화는 북서 태평양 내 태풍의 특성에 오랫동안 영향을 미쳤고, 이로 인해 한반도 연안에서 치명적인 재해가 증가하고 있다. 마이크로파 센서의 일종인 Synthetic Aperature Radar (SAR)는 위성 광학 및 적외선 센서로는 바람을 구할 수 없는, 흐린 대기 조건인 태풍 주위에서 고해상도 바람장을 생산할 수 있다. SAR 자료로부터 해상풍을 산출하기 위한 Geophysical Model Functions (GMFs)에는 풍향 입력이 필수적이며, 이는 태풍 중심을 정확히 추정하는 것에 기반해야 한다. 본 연구는 태풍 중심 탐지 방법의 문제점을 개선하고 이를 해상풍 산출에 반영하기 위하여, Sentinel-1A 영상을 이용해 태풍 중심을 추정하였다. 그 결과는 한국 및 일본 기상청이 제공한 태풍 경로자료와 비교하여 검증하였고, Himawari-8 위성의 적외 영상도 활용하여 검증하였다. 태풍의 초기 중심 위치는 VH 편파를 이용해 설정하여 오차의 발생 가능성을 줄였다. 탐지된 중심은 한국 및 일본 기상청에서 제공하는 4개 태풍의 경로 자료와 평균 23.76 km의 차이를 보였다. Himawari-8 위성에서 추정된 태풍 중심에 비교했을 때 결과는 육지 근처에 위치하면서 58.73 km의 큰 차이를 보인 한 태풍을 제외하고는 평균 11.80 km의 공간 변이를 보였다. 이는 고해상도 SAR 영상이 태풍 중심을 추정하고 태풍 주위 해상풍 산출에 활용될 수 있음을 시사한다.

• Animal Bioscience
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• 제36권8호
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• pp.1241-1251
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• 2023

Objective: Egg yolk immunoglobulin (IgY) is an antibiotic alternative to prevent and fight intestinal pathogenic infections. This study aimed to investigate the effects of sodium alginate/chitosan/sodium alginate IgY microcapsules on the growth performance, serum parameters, and intestinal health of broiler chickens. Methods: One-day-old broilers (Ross 308) were divided into five treatments, each with 10 replicates of five chickens. The dietary treatments were maintained for 28 days and consisted of a basal diet (NC), basal diet + 500 mg chlortetracycline/kg diet (CH), basal diet + 50 mg non-microencapsulated IgY/kg diet (NM), basal diet + 600 mg low levels microencapsulated IgY/kg diet (LM), and basal diet + 700 mg high levels microencapsulated IgY/kg diet (HM). Results: Throughout the 28-day trial period, the NM, LM, HM, and CH groups increased average daily gain compared with the NC group (p<0.05), and the HM group reduced feed conversion ratio compared with the CH group (p<0.05). The LM and HM groups increased relative organ weights of thymus and spleen compared with the CH and NM groups (p<0.05). The HM group improved the duodenal, jejunal and ileum villi height (VH) and villus height to crypt depth ratio (VH:CD) compared with the CH and NM groups (p<0.05). Compared with the CH group, the HM group increased serum immunoglobulin (IgA), immunoglobulin G (IgG), superoxide dismutase, total antioxidant capacity, and glutathione peroxidase levels (p<0.05), and decreased serum malondialdehyde levels (p<0.05). Compared with the NC group, the NM, LM, HM, and CH groups reduced colonic Escherichia coli and Salmonella levels (p<0.05). and the HM group promoted the levels of lactic acid bacteria and bifidobacteria compared with the CH group (p<0.05). Conclusion: Microencapsulation could be considered as a way to improve the efficiency of IgY. The 700 mg high levels microencapsulated IgY/kg diet could potentially be used as an alternative to antibiotics to improve the immune performance and intestinal health, leading to better performance of broiler chickens.