• Title/Summary/Keyword: Velvet antlers

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A study on the extracting characteristics of velvet antlers using kyenegum protease (계내김(鷄內金)에서 추출한 protease를 이용한 녹용(鹿茸) 추출 특성 연구)

  • Park, Jae-Ho;Kim, Do-Wan
    • The Korea Journal of Herbology
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    • v.26 no.4
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    • pp.89-94
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    • 2011
  • Objective : Kyenegum has been frequently used for characterizing digestive symptoms in the traditional and oriental medicines. This study was conducted to investigate the characteristics of extracts from velvet antlers using the 4 different kinds of extracting methods. Methods : The extracts of velvet antlers were extracted using a $65^{\circ}C$ DW (9hrs), a Kyenegum crude enzyme, a $121^{\circ}C$ DW (2hrs), and a Kyenegum protease. To evaluate the characteristic of velvet antler extracts, we examined the brix, soluble solid, amino acid, mineral composition, and collagen protein. Results : As a result of the comparisons of velvet antlers extracted by the traditional extraction and the crude enzyme of kyenegum, the brix and soluble solid showed the higher contents for kyenegum enzymes. Also, mineral contents of the extracted velvet antlers were higher, particularly in Ca and P for those. The contents of collagen protein, hydroxyproline and hydroxylysine, were found to be more than twice in kyenegum protease compared with other extracting methods. Conclusion : These results indicated that the Kyenegum crude enzyme and protease are very effective to extract of velvet antlers.

ANALYSES OF THE EXPRESSION PROFILES OF GENES RESPONSIBLE FOR THE GROWTH OF OSTEOBLAST UPON VELVET ANTLERS TREATMENT (녹용이 조골세포(造骨細胞) 성장에 미치는 유전자 발현 profile 분석에 대한 연구)

  • Lee, Jong-Woo;Kim, Doeg-Kon
    • The Journal of Pediatrics of Korean Medicine
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    • v.16 no.1
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    • pp.39-74
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    • 2002
  • Recently, increased attention has been paid to the growth of the height of children and adolescents. To accelerate growth, velvet antlers are typically used in Oriental medicine. The present study investigated the effects of velvet antlers of velvet antlers on bone growth using the cell line of Human Osteosarcoma (Hos), derived from the bone-generating cells essential to bone growth. In order to give certain stress to this Hos, the medium contained 1% FBS was used for culturing for Hos cell instead of 10% in control. In this condition of which the proliferation had been significantly decreased, the ethanol extract of upper part of velvet antlers was added, As a result, the cells proliferation rate was significantly increased. Using Oligonucleotide DNA microarray, comparison and analyses were done to see what kind of specific genes would be differentially expressed. The result showed that as opposed to the control group, the stressed group indicated a decrease in the expressions of 6 kinds of genes such as, Id1, retinoid X receptor(RXRB) and 14-3-3 epsilon, etc. The velvet antler treated group, as opposed to the control group, showed a decreased in the expressions of 8 kinds of genes such as Id1, etc. and an increase in the expressions of 24 kinds of genes. The number of genes that showed differences in the velvet antler treated group compared with the stressed group was 7 the expression of 1 kind of gene was decreased, and the expressions of 6 kinds of genes were increased. Considering the mechanism by which velvet antlers affected the growth of osteoblast through reviewing the functions of these genes, the following results were attained. The constraint in the proliferation of Hos cells resulting from the medium contained 1% FBS seems to be caused by three important factors: 1) the decrease of the expression of 14-3-3 epsilon involved in the signal transduction and metabolism of growth, 2) the decrease of the expression of Id1 gene involved in the metabolism of bone formation, and 3) the decreased of expression of RXRB gene involved in the metabolism of retinoci acid. It is suggested that the improvement of the cell proliferating effects by velvet antler treatment, in stressed condition si mediated by increment of 6 genes particularly 14-3-3 epsilon, RXRB, and IGF2, with are the crucial factors for the cell growth and differentiation, metabolism of retinoic acid and osteoblast proliferation, respectively.

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Discrimination of velvet antlers' origin using DNA polymorphisms

  • Chung, Hwan-Suck;Lee, Hye-Jeong;Kim, Young-Eun;Shin, Min-Kyu;Hong, Moo-Chang;Kim, Yang-Seok;Bae, Hyun-Su
    • Journal of Evidence-Based Herbal Medicine
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    • v.2 no.1
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    • pp.7-12
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    • 2009
  • Velvet antlers from Cervus elaphus species are one of most famous, expensive and commonly used medicinal materials in traditional oriental medicine. Some distributor had illegal practice of disguising the origin of antlers in Korea market. Therefore, a test to distinguish antler essential to ensure the healthy development of the herbal industry. In this study, the variation in DNA sequences of the mitochondrial ATPase8 and cytochrome-coxidaseI (COI) genes of Cervus elaphus from China, the Republic of Altai, and Canada were evaluated. In addition, the sequence variation among, Rein deer and Cervus elaphus species was also evaluated. Although the sequences of deer from the Republic of Altai and Canada were very similar, polymorphisms that were conserved in each species were observed in the ATPase8 and COI genes. Therefore, these polymorphic markers could be used to distinguish Cervus elaphus antlers from different locations.

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Relationships between Antioxidants and Quality Characteristics from Velvet Antlers of Formosan Sambar Deer

  • Cheng, Shih-Lin;Jian, You-Ling;Chen, Chih-Ming;Liu, Bing-Tsan
    • Food Science of Animal Resources
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    • v.37 no.4
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    • pp.542-551
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    • 2017
  • The quality characteristics of velvet antlers obtained from Formosan sambar deer (Cervus unicolor Swinhoi) (SDVA), harvested from 63 to 81 d during the velvet antler growth period, were evaluated by investigating the relationships between antioxidant levels; including content, activity, and content/activity ratios, and physical properties; including shear force values, color, and Ca content. The hardness of samples from base velvet antler sections increased, and that the color of these samples tended to become reddish-yellow (redder and more yellow), suggesting that the Ca content in the base section of the sample was not ossified yet. Samples from the upper sections of velvet antler showed higher superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) content (3.91 to 1.50 mg/mL, 2.53 to 0.90 mg/mL, and 3.95 to 1.58 mg/mL, respectively) than did samples from the middle and base sections (p<0.05). The activity and content/activity ratios of GPX measured in the upper section were also found to be significantly greater than in the middle and base sections (p<0.05). We further observed that the content and activity of GPX was significantly and negatively correlated with Ca content, shear force values, and the content/activity ratio of this antioxidant (p<0.01). The study findings may serve as a reference index for quality evaluations of velvet antlers of Formosan sambar deer in future.

A Study on Physical Properties About Velvet Antler of the Elk(Cerus canadensis) (엘크 녹용의 물성 특성에 관한 연구)

  • Sung, S.H.;Han, J.H.;Kim, Y.M.;Kim, B.K.
    • Journal of Animal Environmental Science
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    • v.13 no.3
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    • pp.201-210
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    • 2007
  • In this study, physical properties of Elk antler was investigated to develop the optimum drying and packaging methods for improving the antler quality as well as deal with diversify of demand. After the antler was sliced with 5 mm thickness, and the compressive, shear, and tensile stresses were measured at the center and velvet parts of pre-dried and dried antlers after the contained water rate of the dried antlers was maintained below 10%. The results are as follows. 1. Considering the center of pre-dried antlers, the compressive stresses were $60.73\;g/mm^2$, $145.65\;g/mm^2$, and $260.97\;g/mm^2$, respectively at the upper, middle, and lower parts while $70.67\;g/mm^2$, $811.90\;g/mm^2$, $3,235.52\;g/mm^2$, respectively for velvet layer. Considering the center of dried antlers, the compressive stresses were $190.43\;g/mm^2$, $445.81\;g/mm^2$, and $705.86\;g/mm^2$, respectively at the upper, middle, and lower parts while $734.01\;g/mm^2$, $1,238.40\;g/mm^2$, $4,134.03\;g/mm^2$, respectively for velvet layer. 2. For the pre-dried, the shear stresses were $50.24\;g/mm^2$, $294.44\;g/mm^2$, and $423.47\;g/mm^2$, respectively, and $124.14\;g/mm^2$, $367.69\;g/mm^2$, and $425.86\;g/mm^2$, respectively for the dried antlers. 3. The tensile stresses were $13.59\;g/mm^2$, $62.85\;g/mm^2$, and $112.07\;g/mm^2$, respectively for the pre-dried and $77.24\;g/mm^2$, $175.87\;g/mm^2$, and $184.06\;g/mm^2$, respectively for the dried antlers. 4. In the case of drying antlers, the physical characteristics of the antlers was. changed such as moisture evaporation, contraction, and surface hardening. For the center part, the changes of the physical characteristics were more significant at the lower part while at the upper part for the velvet layer. 5. The stress changes of Elk antlers was shown very remarkably according to growth point. Moreover, the stress was clearly higher at velvet layer part to the center part, base parts compared to the upper parts.

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Immuno-stimulating, Anti-stress and Anti-thrombotic Effects of Unossified Velvet Antlers

  • Shin, Kuk-Hyun;YunChoi, Hye-Sook;Lim, Soon-Sung;Won, Do-Hee;Kim, Jae-Kun
    • Natural Product Sciences
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    • v.5 no.1
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    • pp.54-59
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    • 1999
  • Both ethanol and water extracts of unossified velvet antler were found to exhibit a significant immuno-stimulating activity as measured by carbon clearance test in mice, a remarkable anti-fatigue effect in weight-loaded forced swimming performance in mice, a significant anti-stress effect on immobilization in rats. The antler extracts also showed a weak but significant anti-thrombotic activity. These findings are indicative of adaptogenic properties of antlers and their normalizing effects during stressful condition.

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Study on the Drying Method of Velvet Antler using Microwave Oven (전자레인지를 이용한 녹용의 건조방법)

  • 성시흥
    • Journal of Animal Environmental Science
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    • v.8 no.3
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    • pp.177-182
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    • 2002
  • Drying process is very important for commercialization of velvet antlers. So far, artificial drying has mostly depended on experience of farmers resulting in low efficiency of drying, deterioration during drying, and contamination by dust and bacteria. Various drying techniques for high-quality production have currently been commercialized in some developed countries, and one of them is a drying technique using microwave. In this study, application of the technique using an electronic (microwave) oven for drying of velvet antler was investigated. The results of this study are as follows. 1. It was found that the drying period was significantly affected by cornification of velvet antlers. Accordingly, it can be assumed that the velvet antlers should partly be sliced to save drying period and required drying energy. 2. It was also found that the drying speed was changeable according to tempering period and even with same drying period. The tempering period should be controlled according to processing rate. 3. The results indicated that a repeated drying of 2-min drying and 3-min tempering is the most effective when a microwave oven was used. 4. The results also showed that the drying technique using microwave of electronic oven can be effectively used for drying of velvet antlers. 5. Only drying and tempering periods were examined in this study, however, ingredient change of deer antler after drying is necessary to be investigated in the future.

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Endocrinological Studies and Potential Biomedical Uses of Antlers

  • Sunwoo, Hoon H.;Kim, Young K.;Sim, Jeong S.
    • Journal of Life Science
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    • v.12 no.1
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    • pp.1-8
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    • 2002
  • Antlers from deer species are alternative animal by-products. Due to the oriental trade, the velvet antler industry is rapidly emerging in North America. The unique biological property of antler wish a deciduous natural phenomenon offers the valuable model of biomedical research. Growing antlers showed different structures according to cell populations consisting of mesenchymes, chondroblasts, chondrocytes and osseous tissues from distal to proximal portions of main beam. Their structures were different from two tissues, cartilage and bone, in growing antlers. Zone of maturing ants calcifying chondrocytes referred as upper section was invaded by osteoblasts indicating the occurrence of endochondral ossification. The cartilaginous tissues were gradually replaced by osseous tissues downward. The bony tissues referred as the middle and base sections in this thesis contained spongy bone and cortical bone structure in the difference of the degree of mineralization antral the thickness of cortical bony in adjacent to outer velvet layer. In addition, the endocrinological regulators such as testosterone, prolactin, growth hormones and other growth factors are actively involved in the unique deciduous nature shown in the growth and development of antler.

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Changes of Amino Acid, Fatty Acid and Lipid Composition by the Growth Period in Velvet Antler (녹용의 성장 기간에 따른 아미노산, 지방산, 지질성분의 변화)

  • Jeon, Byong-Tae;Moon, Sang-Ho;Lee, Sang-Rak;Kim, Myeong-Hwa
    • Food Science of Animal Resources
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    • v.30 no.6
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    • pp.989-996
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    • 2010
  • This study was aimed at investigating the differences in chemical composition of antler according to growing days (80 d and 90 d after casting) and sections (upper, middle, base) in elk. Antlers of six bulls (aged 3 years) cutted on day 80 and 90 after casting were used in this trial and compared chemical composition such as crude protein, crude fat, crude ash, calcium, magnesium, amino acids, lipids, and fatty acids. The contents of crude protein and ether extract were higher in antlers cutted on day 80 than in antlers cutted on day 90, but that of crude ash was higher in antlers cutted on day 90. Significantly difference was observed in crude protein content between growing days in middle part of antler (p<0.05). Calcium and magnesium contents were higher on 90 d than 80 d, but there was no significant difference between growing days of antler. Based on the growing period of antler, there were significant differences (p<0.05) in aspartic acid, threonine, valine, leucine, phenylalanine and lysine, and essential amino acids content was higher in antlers cutted on day 80. Total lipid, neutral lipid, and phospholipid contents were higher in antlers cutted on day 80 than in those cutted on day 90, sphingo-phospholipid content was higher than glycerophospholipid content and values was higher in antlers cutted on day 80 than in those cutted on day 90. For total fatty acids, unsaturated fatty acids composition, there was no significant difference between growing periods of antlers, but values was higher on day 80 than on day 90. Essential fatty acids contents were lower in antler cutted on day 90 than antlers cutted on day 80 and higher for upper part than lower part.

A Study on the Change of Blood Constituents During Growth Period of Velvet Antler in Korean Spotted Deer (꽃사슴의 녹용 성장기간 중 혈액성분의 변화에 관한 연구)

  • Kim, M.H.;Kim, Y.S.;Jeon, B.T.;Moon, S.H.
    • Korean Journal of Pharmacognosy
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    • v.34 no.3 s.134
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    • pp.263-268
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    • 2003
  • This study was carried out to evaluate the change of blood constituents during the growth of antlers in Korean spotted deer (Cervus nippon). Samples of blood, obtained from the jugular vein of twenty five deer (3 to 6 year-old males) were taken in 10 days interval from casting to cutting of antler and were analyzed the blood parameters. Total-protein concentration in blood serum showed significantly differences during growth period of velvet antler (p<0.01), and it had similar tendency for total protein and albumin concentration, but albumin concentration was about 40-45% of total protein concentration. Urea and creatinine concentration was tended to inverse proportion, and it was almost 30:1 for ratio of urea and creatinine concentration. The concentration of total protein, creatinine, and uric acid concentration in blood during growth period of velvet antler was similarly increased and decreased but urea concentration was opposed. The concentration of total-bilirubin and direct-bilirubin at casting was higher than those at other period(P<0.05).