• Animal Bioscience
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• 제35권2호
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• pp.166-176
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• 2022

Objective: Sperm is particularly susceptible to reactive oxygen species (ROS) stress. Glutathione (GSH) is an endogenous antioxidant that regulates sperm redox homeostasis. However, it is not clear whether boar sperm could utilize cysteine for synthesis GSH to protect sperm quality from ROS damage. Therefore, the present study was undertaken to elucidate the mechanism of how cysteine is involved in protecting boar sperm quality during liquid storage. Methods: Sperm motility, membrane integrity, lipid peroxidation, 4-hydroxyIlonenal (4-HNE) modifications, mitochondrial membrane potential, as well as the levels of ROS, GSH, and, ATP were evaluated. Moreover, the enzymes (GCLC: glutamate cysteine ligase; GSS: glutathione synthetase) that are involved in glutathione synthesis from cysteine precursor were detected by western blotting. Results: Compared to the control, addition of 1.25 mM cysteine to the liquid storage significantly increased boar sperm progressive motility, straight-line velocity, curvilinear velocity, beat-cross frequency, membrane integrity, mitochondrial membrane potential, ATP level, acrosome integrity, activities of superoxide dismutase and catalase, and GSH level, while reducing the ROS level, lipid peroxidation and 4-HNE modifications. It was also observed that the GCLC and GSS were expressed in boar sperm. Interestingly, when we used menadione to induce sperm with ROS stress, the menadione associated damages were observed to be reduced by the cysteine supplementation. Moreover, compared to the cysteine treatment, the γ-glutamylcysteine synthetase (γ-GCS) activity, GSH level, mitochondrial membrane potential, ATP level, membrane integrity and progressive motility in boar sperm were decreased by supplementing with an inhibitor of GSH synthesis, buthionine sulfoximine. Conclusion: These data suggest that boar sperm could biosynthesize the GSH from cysteine in vitro. Therefore, during storage, addition of cysteine improves boar sperm quality via enhancing the GSH synthesis to resist ROS stress.

• 한국운동역학회지
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• 제25권4호
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• pp.483-488
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• 2015

Objective : The purposes of this study was to analyze the locomotive movement of forelimbs according to changes in velocities in the quadruped cadence of horses. Methods : Horses selected as subjects consisted of Jeju pony horses (heights of withers: $1.23{\pm}0.51$). Two camcorders (HDR-HC7/HDV 1080i, Sony Corp, Japan) were used to capture the movement of the horses' forelimbs at a rate of 60 frames/sec. Additionally, raw data was collected from Kwon3D XP motion analysis package ver 4.0 program (Visol, Korea) with DARTFISH (DFKREA., Korea) video software solution. The variables analyzed consisted of 1 step lengths, 1 stride lengths, stance time, swing time, 1 stride time, velocity while walking, and trot of the horses. A two-way ANOVA and paired t-test of the variables by velocity and phase were treated at .05 level of significant difference, statistically. Results : The time elapsed of walk(stance: 0.63 sec[63.86%], swing: 0.35 sec[36.14%], 1 stride time: 0.99 sec respectively) showed significant difference with more delay than that of trot(stance: 0.29 sec[45.73%], swing: 0.34 sec[54.27% ], 1 stride time: 0.63 sec respectively), and also showed significant difference at trot in interaction (stance time>tort swing>walk swing>walk stance). The 1 step lengths and stride lengths in trot showed significant difference with longer than that of walk. Velocity of Trot showed significant difference statistically with higher than that of walk Conclusion : The horses' velocity during 1 step lengths and 1 stride lengths showed a proportional relationship, but the correlation between the horses' velocity and stance time showed a negative relationship during the quadruped cadence.

• 한국지열·수열에너지학회논문집
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• 제12권1호
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• pp.1-6
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• 2016

Characteristics of exhaust from chimney of electricity generator are analyzed based on CFD when Waveguide-Below-Cutoff (WBC) array is installed in order to achieve the certain level of electromagnetic pulse (EMP) shielding. The main purpose is prediction of average and maximum velocity of exhaust. The results reveal that: 1) When the specification of waveguide is given as 80-diameter, 400-length, and the gap of 20 mm, the shielding effectiveness (SE) is 140dB. The average and maximum velocity of exhaust in the chimney with WBC Array can be represented as exponential functions. 2) As the number of WBC increases, the velocity in the chimney dwindles. 3) Under the situation that WBC with 80 mm diameter is located at intervals of 20 mm, the average velocity can be approximated by $25.5344{\times}e^{(-0.0098{\times}N_{WBC})}$ with input velocity of 15 m/s. In addition, the determination coefficient is 0.915, which is sufficiently high.

• 지구물리와물리탐사
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• 제9권3호
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• pp.193-206
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• 2006

실체파 속도 결정 목적의 여러 시추공 탄성파 시험 기법 중에서 크로스홀 기법은 지반 동적 특성 평가에 신뢰성이 가장 높은 결과를 도출할 수 있는 기법 중의 하나로 알려져 있다. 이에 본 연구에서는 지하수위 존재 여부에 관계 없이 토사 뿐 만 아니라 암반을 대상으로 크로스홀 탄성파 시험을 성공적으로 수행할 수 있도록, 연직 시추공 안에서 지반에 대한 수평 방향 가진이 가능한 다목적의 스프링식 발진 장치를 개발하고, 국내 주요 시설물 부지들을 대상으로 크로스홀 탄성파 시험을 실시하였다. 대상 부지에서의 수평방향 가진의 크로스홀 탄성파 시험으로부터 전단파 속도 및 압축파 속도와 같은 실체파 속도의 결정을 통해 지반 동적 특성을 효율적으로 평가하였으며, 적용 대상인 시설물들의 내진 성능 평가 및 내진 설계를 위한 근본 자료로 제시하였다.

• 한국연소학회:학술대회논문집
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• 한국연소학회 제26회 KOSCO SYMPOSIUM 논문집
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• pp.195-199
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• 2003

The stoichiometric methan/air premixed turbulent flames at the axisymmetric Bunsen burner situation are numerically investigated. To account for the chemistry-turbulence interaction in the turbulent premixed flames, the steady laminar flamelet library method has been adopted. The flame front is tracked by using the Level-Set Approach. Turbulence is represented by the ${\kappa}-{\varepsilon}$ modeling with a Pope's correction. The detailed comparison between prediction and measurement has made for the flame field in terms of velocity, turbulent kinetic energy, and normarlized temperature.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2004년도 춘계 학술발표회 제16권1호
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• pp.700-703
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• 2004

The purpose of this study is to present the design methodology of base isolated buildings. To achieve the goal of this study, time-history analysis was performed with seismic performance level and recorded seismic data. From the analysis results of MDOF system, the maximum. displacement and base shear were evaluated as 25 cm and $4\%$ by the input level which is maximum velocity of 50 kine. By introducing hybrid isolation system, seismic energy can be concentrated consequently high seismic capacity of the total building is secured.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2001년도 제22회 KOSCI SYMPOSIUM 논문집
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• pp.82-89
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• 2001

This paper concerns lean gas cyclone combustion system adopting distributed inlets with different velocity to promote ignition and burnout properties. Detailed temperature measurements have been achieved under different operating conditions and flue gas compositions and NOx have been measured. Experimental results show that cyclone combustor provided increasing combustion stability and reduction NOx emission level to negligible level.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 합동 추계학술대회 논문집 정보 및 제어부문
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• pp.95-98
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• 2001

A hierarchical structure for the cooperative control of multiple mobile robots using coordinates of objects obtained from vision system is proposed. The order-level perceives environments represented by workspace sets. The algorithm selects an object to be moved using an object discrimination part and determines the robot actions. The action-level generates a trajectory of each wheel velocity of robot. The simulation results show the effectiveness of the proposed algorithm.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 1999년도 춘계 학술발표회 논문집 Proceedings of EESK Conference-Spring
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• pp.85-92
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• 1999

Blasting induced damage boundary was determined by measuring vibrations adjacent to charging holes. the criterion adopted to define damages is that blasting-induced strains exceeding tension-crack strain level cause damages. The blasting vibrations were measured in terms of acceleration and converted to strains. The tension-crack strain level was determined with tensile strengths and elastic moduli of rock cores. The damage zone was found to be extended radially about 1 meter from the blasthole detonated with 250 to 700 grams of explosives. The comparison of shear wave velocity profiles before and after blasting shows that the damage boundary of 1 meter seems to be reliable.

• Journal of the Korean Statistical Society
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• 제3권1호
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• pp.17-30
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• 1974

There are a number of studies on the peculiarities of inflation in underdeveloped economies. These studies are, however, confined to the cases of Latin American countries, and the essence of methodologies applied in the studies is basically estimations of linear parameters of an extended quantity equation with lagged variables. As it is generally observed, inflation in most of underdeveloped economies are, to some extent, affected by non-economic factors such as political instability, social disorder, abrupt institutional changes, etc. Sometimes, these factors underlying the basic movement of price level change are reflected in such variables as quantity of money supply, income velocity, gross national product.