• Nutrition Research and Practice
• /
• v.11 no.3
• /
• pp.190-197
• /
• 2017

BACKGROUND/OBJECTIVES: Gallus gallus domesticus (GD) is a natural mutant breed of chicken in Korea with an atypical characterization of melanin in its tissue. This study investigated the effects of melanin extracts of GD on osteoblast differentiation and inhibition of osteoclast formation. MATERIALS/METHODS: The effects of the melanin extract of GD on human osteoblast MG-63 cell differentiation were examined by evaluating cell viability, osteoblast differentiation, and expression of osteoblast-specific transcription factors such as bone morphogenetic protein 2 (BMP-2), small mothers against decapentaplegic homologs 5 (SMAD5), runt-related transcription factor 2 (RUNX2), osteocalcin and type 1 collagen (COL-1) by reverse transcription-polymerase chain reaction and western blotting analysis. We investigated the inhibitory effect of melanin on the osteoclasts formation through tartrate-resistant acid phosphatase (TRAP) activity and TRAP stains in Raw 264.7 cell. RESULTS: The melanin extract of GD was not cytotoxic to MG-63 cells at concentrations of $50-250{\mu}g/mL$. Alkaline phosphatase (ALP) activity and bone mineralization of melanin extract-treated cells increased in a dose-dependent manner from 50 to $250{\mu}g/mL$ and were 149% and 129% at $250{\mu}g/mL$ concentration, respectively (P < 0.05). The levels of BMP-2, osteocalcin, and COL-1 gene expression were significantly upregulated by 1.72-, 4.44-, and 2.12-fold in melanin-treated cells than in the control cells (P < 0.05). The levels of RUNX2 and SMAD5 proteins were higher in melanin-treated cells than in control vehicle-treated cells. The melanin extract attenuated the formation of receptor activator of nuclear factor kappa-B ligand-induced TRAP-positive multinucleated RAW 264.7 cells by 22%, and was 77% cytotoxic to RAW 264.7 macrophages at a concentration of $500{\mu}g/mL$. CONCLUSIONS: This study provides evidence that the melanin extract promoted osteoblast differentiation by activating BMP/SMADs/RUNX2 signaling and regulating transcription of osteogenic genes such as ALP, type I collagen, and osteocalcin. These results suggest that the effective osteoblastic differentiation induced by melanin extract from GD makes it potentially useful in maintaining bone health.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.11
• /
• pp.13-18
• /
• 2019

The DEM(Digital Elevation Model) is a three-dimensional spatial information that stores the height of the terrain as a numerical value. This means the elevation of the terrain not including the vegetation and the artifacts. The DEM is used in various fields, such as 3D visualization of the terrain, slope, and incense analysis, and calculation of the quantity of construction work. Recently, many studies related to the construction of 3D geospatial information have been conducted, but research related to DEM generation is insufficient. Therefore, in this study, a DEM was constructed using a MMS (Mobile Mapping System), UAV image, and UAV LiDAR (Light Detection And Ranging), and the accuracy evaluation of each result was performed. As a result, the accuracy of the DEM generated by MMS and UAV LiDAR was within ± 4.1cm, and the accuracy of the DEM using the UAV image was ± 8.5cm. The characteristics of MMS, UAV image, and UAV LiDAR are presented through a comparison of data processing and results. The DEM construction using MMS and UAV can be applied to various fields, such as an analysis and visualization of the terrain, collection of basic data for construction work, and service using spatial information. Moreover, the efficiency of the related work can be improved greatly.

• The Korean Journal of Rheology
• /
• v.10 no.4
• /
• pp.217-226
• /
• 1998

The anisotropy caused by the fiber orientation, which is inevitably generated by the flow during injection molding of short fiber reinforced polymers, greatly influences dimensional accuracy, mechanical properties, and other quality of the final product. Since the filling stage of the injection molding process plays a vital role in determining fiber orientation, an accurate analysis of flow field for the filling stage is needed. Unbalanced filling occurs when a complex or a multi-cavity mold is used leading to development of regions where the fiber suspension is under compression. It is impossible to make an accurate calculation of the flow field during filling with the analysis assuming incompressible fluid. A mold with four cavities with different filling times was produced to compare the numerical analysis results with the experimental data. There was a good agreement between the experimental and theoretical results when the compressibility of the polymer melt was considered for the numerical simulation. The fiber orientation states for compressible and incompressible fluids were also compared qualitatively as well as quantitatively in this study.

• Journal of Korean Society of Steel Construction
• /
• v.29 no.6
• /
• pp.467-477
• /
• 2017

The live load effect of KL-510 of the current Korean bridge design code is examined by comparing with that of the multiple trucks of which the weights are statistically estimated from measured traffic data as well as with those of the related live load models. The truck weight data measured on the expressway before and after overweight enforcement are used to obtain the truck weights following the same procedures in deciding the live load model of the design codes and the results are compared with the load effect of KL-510. KL-510 yields a very uniform loading effect compared with the multiple truck effects when the weights are estimated from the data which contains some of the heavy trucks over the operational weight limit. KL-510 yields consistent results with the live load of AASHTO LRFD and shows less variation than the past load model DB-24 over the span lengths considered in this study. As a result of this research, the actual truck combinations equivalent to the notional KL-510 load model are constructed and it can be applied to the evaluation of the existing bridge and the calibration of the load factor of the permit vehicle.

• Journal of Korean Society of Transportation
• /
• v.20 no.3
• /
• pp.17-30
• /
• 2002

Incident duration prediction is one of the most important steps of the overall incident management process. An accurate and reliable estimate of the incident duration can be the main difference between an effective incident management operation and an unacceptable one since, without the knowledge of such time durations, traffic impact can not be estimated or calculated. This research presents several multiple linear regression models for incident duration prediction using data consisting of 384 incident cases. The main source of various incident cases was the Traffic Incident Reports filled out by the Motorist Assistant Units of the Korea Highway Corporation. The models were proposed separately according to the time of day(daytime vs. nighttime) and the fatality/injury incurred (fatality/injury vs. property damage only). Two models using an integrated dataset, one with an intercept and the other without it, were also calibrated and proposed for the generality of model application. Some findings are as follows ; ?Variables such as vehicle turnover, load spills, the number of heavy vehicles involved and the number of blocked lanes were found to significantly affect incident duration times. ?Models, however, tend to overestimate the duration times when a dummy variable, load spill, is used. It was simply because several of load spill incidents had excessively long clearance times. The precision was improved when load spills were further categorized into "small spills" and "large spills" based on the size of vehicles involved. ?Variables such as the number of vehicles involved and the number of blocked lanes found not significant when a regression model was calibrated with an intercept. whereas excluding the intercept from the model structure signifies those variables in a statistical sense.

• Journal of Korean Society of Transportation
• /
• v.36 no.3
• /
• pp.169-183
• /
• 2018

Aggressive driving leads to a greater crash potential because it threatens surrounding vehicles. This study conducted traffic simulation experiments using driving behavior data obtained from multi-agent driving simulations. VISSIM traffic simulator and surrogate safety assessment model (SSAM) were used to identify the impacts of aggressive driving on traffic stream in terms of safety and operational efficiency. Market penetration rates (MPR) of aggressive driving vehicle, coupled with various traffic conditions, were taken into consideration in analyzing the impacts. As expected, it was identified that aggressive driving vehicles tended to deteriorate the traffic safety performance. From the perspective of operational efficiency, interesting results were observable. Under level of service (LOS) A, B, and C, it was observed that the average travel speed increased with greater MPRs. Conversely, the average travel speed decreased with under LOS D and E conditions. The outcome of this study would be effectively used for developing safety-related policies for reducing aggressive driving behavior.

• Journal of Chest Surgery
• /
• v.31 no.3
• /
• pp.291-297
• /
• 1998

Sternal fractures, once thought of as an uncommon phenomenon, have occurred with an increasing frequency, paralleling the incidence of motor vehicle accidents. The tremendous force necessary to cause sternal fracture and this bone's prominent position overlying major intrathoracic and mediastinal structures, have important implications in the assessment and treatment of patients. This evaluation is based on the review of 72 patients of traumatic sternal fracture treated at the Department of Thoracic and Cardiovascular Surgery, Seoul Adventist Hospital during the last 4 years from March 1993 to February 1997. The frequency was 12.2% of nonpenetrating chest trauma and average age was 43.2 years old. Automobile accidents(84%) and sternal body fractures(95.8%) with anterior displacements(19.4%) was the most common cause and fracture site. Increase of cardiac isoenzymes was more frequent and higher in sternal fracture than chest contusion but there was no relationship between the time to take normalization of them and the mode of trauma.

• Microbiology and Biotechnology Letters
• /
• v.36 no.1
• /
• pp.72-81
• /
• 2008

The effect of genistein on melanin synthesis was studied using in vitro and in vivo model. Genistein inhibited melanin synthesis in cultured melan-a cells dose dependently. Tyrosinase activity was decreased by genistein treatment in melan-a cells, but genistein did not inhibit tyrosinase directly. Genistein did not affect the expression of tyrosinase in melan-a cells. Genistein inhibited the activity of ${\alpha}$-glucosidase in virtro and the glycosylation of tyrosinase in melan-a cells. The resulting unsaturated glycosylation of tyrosinase makes it unstable and disturb correct transportation. To further clarify the effect of genistein on the melanogenesis, we established UVB-induced hyperpigmentation on the shaved backs of brown guinea pigs. The animals were exposed to UVB radiation once a week for three consecutive weeks. Genistein (1 and 2%) or vehicle alone as a control were then topically applied to the hyperpigmented areas daily. Genistein showed significant lightening effect on the UVB-induced hyperpigmentation in five weeks. Depigmenting effect was prominent in 2% genistein treatment with Fontana-Masson staining. In conclusion, genistein may be a useful agent for skin whitening.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.2
• /
• pp.97-104
• /
• 2018

Recently, many studies have examined UAVs (unmanned aerial vehicles), which can replace and supplement existing surveying sensors, systems, and images. This study focused on the use of UAV images and assessed the possibility of utilization in areas where it is difficult to obtain GCPs (ground control points), such as disasters. Therefore, 3D (dimensional) pointcloud data were generated using UAV images and the absolute/relative accuracy of the generated model data using GCPs and without GCPs was assessed. The results showed the 3D shape pointcloud generated by UAV image matching was proven if the relative accuracy was set, regardless of whether GCPs were used or not; the quantitative measurement error rate was within 1%. Even if the absolute accuracy was low, the 3D shape pointcloud that had been post processed quickly was sufficient to be utilized when it is impossible to acquire GCPs or urgent analysis is required. In particular, the results can obtain quantitative measurements and meaningful data, such as the length and area, even in cases with the ground reference point surveying and post-process.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.11 no.2
• /
• pp.709-719
• /
• 2010

As the world has had a high recognition of security since 9/11 attacks in 2001, many countries and international organizations around the world are making strenuous efforts in establishing stronger security, centering on areas with high possibility of terrorist attacks. In particular, harbor facilities where a great deal of trade take place between countries, have high possibilities of becoming the objects of terrorist attacks and also of becoming places of trading objects and illegal items which might be leveraged for terror attacks. In this study, I have explained the current status of the harbor entrance system through existing documents, interviews and field trips to harbors and have also introduced improvement measures using RFID technology from the viewpoint of BP(Business Process). I expect that this study will be used as basic materials because I have studied the number of people entering harbors and working procedures for vehicle entrance, which have not been studied so far and also laid out various factors to be considered to introduce RFID technology.