• 한국임상약학회지
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• 제6권2호
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• pp.32-40
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• 1996

The electrophysiological effects of benzopyran potassium channel openers (PCOs: lemakalim, KR-30450 and KR-30818) on the ischemia/reperfusion-induced arrythmias were investigated. In anesthetized rats, subjected to 45 min occlusion of the left anterior descending coronary artery (LAD) followed by 90 min reperfusion, ventricular arrythmias were identified according to the Lambeth Conventions by lead II ECG. Rats were intravenously given vehicle ($1\%$ DMSO), lemakalim, KR-30450, and KR-30818 alone or in combination with a selective $K_{ATP}$ blocker glibenclamide, 30 min prior to coronary occlusion. Compared to vehicle, lemakalim ($30{\mu}g/kg$ i.v.), the active enantiomer of cromakalim, had a tendancy to increase the duration of ventricular tachycardia (Vl) and ventricular fibrillation (VF), the number of premature ventricular complexes (PVC) and the incidence of VF, especially in the early post-occlusion peroid ($0\~15$ min), while increasing ST-segment elevation. Both KR-30450 ($30{\mu}g/kg$, i.v.) and KR-30818 (30, $100{\mu}g/kg$, i.v.) showed similar proarrhythmic effects to lemakalim (PVC, duration of VT, and incidence of VF) with a tendancy to decrease the duration of VF and ST-segment elevation. Unlike other PCOs, however, glibenclamide (0.3, 1.0 mg/kg) had opposite effects on the induction of arrhythmias (PVC, the duration of VF); it had a tendancy to increase the duration of VT with a slight elevation of ST-segment. It seems likely that glibenclamide (0.3 mg/kg, i.v.), reduced the effects of lemakalim or KR-30450 ($30{\mu}g/kg$, i.v.) on arrhythmias (PVC, VT, VF and ST-segment). These results indicate that, in the coronary occluded rat model of ischemia, lemikuiln and KR-30450 exert a proarrhythmic activity, the effect being considered related to the opening of KATP channel.

• 한국통신학회논문지
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• 제41권12호
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• pp.1868-1875
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• 2016

본 논문에서는 항공 ICT분야에서 최근 이슈가 되고 있는 UAV(Unmanned Aerial Vehicle)에 대한 효율적인 연구 개발 전략 수립을 위하여 특허분석을 기반으로 기술의 메가트렌드 및 경쟁력 분석 결과를 제시한다. UAV 핵심 기술의 메가트렌드 분석을 위해 한국, 미국, 일본 및 유럽의 공개/등록 특허를 중심으로 특허 동향을 분석하였다. 분석 대상 기술은 UAV와 ICT기술이 융합이 되는 기술을 중심으로 3개의 소분류로 구분하였으며, 특허검색 결과 총 3,433건이 검색되었다. UAV 핵심 기술별 경쟁력 및 한국의 경쟁력을 분석하기 위하여 특허 활동력, 특허 피인용률 및 주요 시장 확보율을 분석 지표로 사용하였다. 각 핵심 기술에 대한 R&D 전략 영역을 제시하고 이를 기반으로 각 영역별 연구개발 전략을 제시하였다. 본 논문을 통해 우리나라의 기술 수준, 선진 기업의 연구 개발동향 및 핵심특허 현황 등을 객관적인 특허정보를 기반으로 분석하였으며, 향후 UAV 분야의 연구개발 및 특허 확보 전략 수립에 활용이 될 것으로 기대 한다.

• 대한한의학회지
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• 제31권5호
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• pp.90-102
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• 2010

Objectives: This study was investigated the protective effect of Spatholobi Caulis water extract against cadmium (CdCl2, Cd)-induced hepatic toxicity in rats. Methods: To induce acute hepatic toxicity, Cd (4 mg/kg body weight) was dissolved in normal saline and intravenously injected into rats. Then, the rats received either a vehicle or silymarin (100 mg/kg) or Spatholobi Caulis water extract (30, 50 mg/kg/day) for 3 days, and were exposed to a single injection of Cd 24 h after the last Spatholobi Caulis/vehicle treatment. Results: Alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were significantly increased by Cd treatment. In contrast, pretreatment with Spatholobi Caulis reduced ALT, AST and LDH. Cd-intoxicated liver damage was significantly inhibited by treatment of Spatholobi Caulis 30 and 50 mg/kg at histopathological observations in the present study. Conclusions: These results can be considered as direct evidence that Spatholobi Caulis has favorable inhibitory effects on the Cd-intoxicated liver damages. The efficacy of Spatholobi Caulis 30 mg/kg shows similar effects to that of silymarin 100 mg/kg, and more favorable hepatoprotective effects were observed in Spatholobi Caulis 50 mg/kg as compared with silymarin 100 mg/kg against Cd-intoxicated hepatopathies in the present study.

• 약학회지
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• 제41권1호
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• pp.117-125
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• 1997

The pharmacological effects of benzopyran potassium channel openers (lemakalim, KR-30450 and KR-30818) on the occlusion/reperfusion-induced myocardial infarction were investigat ed. In anesthetized rats, subjected to 45-min occlusion of the left anterior descending coronary artery (LAD) followed by 90-min reperfusion, the infarct size was measured by calculating the ratio of infarct zone to area at risk (IZ/AAR) with the Evans blue/TTC technique. Rats were intravenously given vehicle (1% DMSO), lemakalim, KR-30450, and KR-30818 alone or in combination with a selective K$_{ATP}$ blacker glibenclamide, 30 min prior to coronary occlusion. Compared to vehicle, lemakalim (30 ${\mu}$g/kg i.v.), the active enantiomer of cromakalim, had a tendancy to decrease infarct size. KR-30450(30 ${\mu}$g/kg, i.v.). the newly synthetized potassium channel openers (PCOs), caused a reduction of infarct size (from 70${\pm}$4%to 57${\pm}$5%). but KR-30818 (30 ${\mu}$g/kg, i.v.), a metabolite of KR-30450. did not modify infarct size. It seem ed likely that glibenclamide (0.3mg/kg, i.v.), given in combination, reduced the effects of these PCOs, especially KR-30450 (30 ${\mu}$g/kg, i.v.) on the infarct size. These results indicate that. in the coronary occluded rat model of ischemia, lemakalim and KR-30450 may exert cardioprotective activity through a reduction of infarct size, the effect being considered related to the opening of K$_{ATP}$ channel.

• 약학회지
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• 제41권6호
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• pp.817-828
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• 1997

In order to investigate pharmacological properties of New Woohwangchungsimwon Pill (NWCH) and Woohwangchungsimwon Pill(WCH), effects of NWCH and WCH on cerebral ischemia and central nervous system were compared. Cerebral ischemia insult was performed using unilateral carotid artery occlusion in Mongolian gerbils. The histological observations showed preventive effect of NWCH and WCH treatments with ischemia-induced brain damage. The ATP in brain tissue was decreased in vehicle-treated ischemic gerbils. This decrease was prevented by NWCH and WCH treatment. In contrast to what was seen with ATP, the lactate and lipid peroxide were both elevated in vehicle-treated ischemic gerbils. This elevation was inhibited by NWCH and WCH treatments. In central nervous system, NWCH and WCH had sedative effect in rotarod and spontaneous activity test, but no effects on the hexobarbital-induced sleeping time. And, NWCH and WCH had weak anticonvulsion effects in electric shock- and pentetrazol-induced convulsion test. NWCH and WCH increased the respiration rate, but decreased the respiration depth in rats. Furthermore, NWCH and WCH showed antistress effect. Our findings suggest that the pharmacological profiles of NWCH on cerebral ischemia and central nervous system are similar to that of WCH.

• 산학경영연구
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• 제13권
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• pp.263-273
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• 2000

벤츠(현 다임-클라이슬러)자동차회사는 새로운 공장을 미국 앨라배마 주 Vance 지역에 건설하고 이곳 공장으로부터 스포츠-레저용의 4륜 구동 M-class 자동차를 생산하고 있다. 이 벤츠공장의 생산라인은 차체형성공정, 도장공정, 그리고 엔진조립 공정으로 나누어져 있고 이들 생산라인사이에는 한 라인에서의 고장(down)이 다른 라인에 파급되지 않도록 완충라인(Buffer)이 설치되어 있다. 본 연구의 목적은 일정한 비율의 고장률을 각 생산라인에 적용시켰을 때 주어진 생산목표(270대)를 달성할 수 있는지, 만약 달성할 수 없다면 가장 문제가 되는 생산라인은 어디인지, 또 생산라인 사이에 존재하는 완충라인이 어느 정도의 완충역할을 하는지, 최적의 생산능력을 유지하기 위한 완충라인의 크기는 어느 정도인지 등, 각종 생산라인의 운영지침(operation policy)들을 시뮬레이션 기법을 이용하여 탐색한 후 병목생산시설(bottleneck)을 찾아내는 것이다. 본 연구를 위한 시뮬레이션 도구로는 ARENA를 이용했고 20일 간의 시뮬레이션 결과를 종합하여 분석하였다. 또 신뢰구간 95% 범위에서 각 생산라인의 최대 생산능력도 측정하여 실제 생산대수와의 차이를 규명할 수 있는 근거를 제공하였다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 추계학술대회 논문집(제1권)
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• pp.379-384
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• 2006

본 연구에서는 세라믹 담체의 기본 성능인 파단계수에 미치는 시험편 치수와 시험 방법의 영향을 평가한 것이다. 중심합성계획에 의해 시험편 치수와 4점 굽힘 시험조건을 설계변수로 선정하여 실험을 수행하고 반응표면법을 이용하여 파단계수에 대한 회귀모델을 구성하였다. 치수효과는 반응표면모델에서 최소 및 최대값의 비를 치수 수정계수로 정의하여 세라믹 담체의 강도 설계에 필요한 파단계수를 제안하였다.

• 한국방사선학회논문지
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• 제15권6호
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• pp.833-839
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• 2021

한반도 비핵화 가능성이 언급되고 있는 상황에서 지리적, 언어적 이점을 고려할 때 한국의 역량을 키워 비핵화 검증에 참여 의지를 나타낼 필요가 있다. 문헌 조사를 통해 북한의 비핵화 대상 시설의 정보 및 현황을 파악하고 주요 비핵화 대상 시설이 위치한 영변 핵단지의 기후 환경, 지리 특성, 교통 여건 등 환경 분석을 수행하였다. 또한, 주요 비핵화 대상 시설인 영변 핵단지의 비핵화 검증을 위한 인력 및 기기의 운반을 위한 시나리오 개발을 위한 접근성 분석을 수행하였다. 접근성 분석은 항공, 항만, 차량 운반으로 구분하여 수행하였으며, 경로별 거리, 예상 소요시간, 경유 도로 등을 분석하였다. 이 과정에서 차량 운반 시나리오의 적합성을 확인할 수 있었으며, 운반 과정에 영향을 미칠 수 있는 인자들과 북한의 상세 도로 현황을 확인하였다. 후속 연구를 통해 최적의 운반 시나리오를 개발하고자 한다.

• 한국동물생명공학회지
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• 제34권1호
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• pp.20-29
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• 2019

Soy isoflavones have been reported to possess many physiological activities such as antioxidant activity and inhibition of cancer cell proliferation. This study investigated the photoprotective effects of soybean extract in human fibroblast cell line and hairless mice model. Human fibroblast was treated with soybean extract before and after ultraviolet B (UVB; 290-302 nm) irradiation. In the soybean extract treated group, the cells showed better resistance to ultraviolet (UV) than control group. The amount of type I collagen recovered from the soybean treated group was higher than the vehicle group exposed to UV-induced damage. Moreover, increased expression of metalloproteinases-1 as a result of UV irradiation was suppressed by the soybean extract. Female mice were orally administered soybean extract and irradiated with UVB light for 8 weeks. The effects of the soybean extract on the skin appearance, collagen deposition and epidermal thickness in the UV-damaged mouse skin were analyzed using histopathological methods. In soybean extract treated group, the skin had a better morphology than that of the control group. Furthermore, the amount of type I collagen was increased and overexpression of MMP-1 was reduced in the soybean extract group compared to vehicle group. Additionally, up-regulation of pro-inflammatory cytokines induced by UV irradiation was suppressed by dietary soybean extract treatment. It appears that soybean extract had a photoprotective effect, including anti-aging and anti-inflammatory effect, from UV-induced damage in not only human fibroblast, but also hairless mice. We confirmed that these effects were possibly due to promotion of collagen synthesis and inhibition of MMP-1 expression.

• 한국축산식품학회지
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• 제43권4호
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• pp.703-711
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• 2023

As an initial study to elucidate the molecular mechanism of how probiotics modulate macrophage activity, we monitored mRNA expression patterns in peritoneal macrophages (PMs) treated with two different strains of probiotics. After treatment with either Weissella cibaria WIKIM28 or Latilactobacillus sakei WIKIM50, total RNAs from PMs were isolated and subjected into gene chip analyses. As controls, mRNAs from vehicle (phosphate-buffered saline, PBS)-treated PMs were also subjected to gene chip analysis. Compared to vehicle (PBS)-treated PMs, WIKIM28-treated and WIKIM50-treated PMs exhibited a total of 889 and 432 differentially expressed genes with expression differences of at least 4 folds, respectively. Compared to WIKIM28-treated PMs, WIKIM50-treated PMs showed 25 up-regulated genes and 21 down-regulated genes with expression differences of more than 2 folds. Interestingly, mRNA transcripts of M2 macrophage polarization marker such as anxa1, mafb, and sepp1 were increased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. Reversely, mRNA transcripts of M1 macrophage polarization marker such as hdac9, ptgs2, and socs3 were decreased in WIKIM50-treated PMs comparing to those in WIKIM28-treated PMs. In agreement with these observations, mRNA expression levels of tumor necrosis factor-α and interleukin-1α were significantly reduced in WIKIM50-treated macrophages compared to those in WIKIM28-treated macrophages. These results may indicate that probiotics can be classified as two different types depending on their ability to convert macrophages into M1 or M2 polarization.