• Title/Summary/Keyword: Vegetative state

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A Study on the Lysis of a Bluegreen Alga Anabaena cylindrica by a Bacterium (세균에 의한 남조 Anabaena cylindrica의 분해에 대한 연구)

  • Kim, Cheol-Ho;Gwon, O-Seop;Lee, Jin-Ae
    • ALGAE
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    • v.18 no.4
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    • pp.355-360
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    • 2003
  • A Gram (-), rod-shaped bacterium in size of 1.6-2.8 $\times$ 0.4 μm was isolated from a eutrophic reservoir, which exhibited growth-inhibiting effect against a bluegreen alga (Anabaena cylindrica). This isolate showed positive reactions for catalase and oxidase, and optimal conditions of 35-40°C and pH 9.0. This isolate was designated AC-1 in this manuscript. In a mixed-culture of A. cylindrica and AC-1, their growth patterns were inversely correlated and the bluegreen algal vegetative cells completely disappeared within 24-36 hours. AC-1 showed similar lytic activity in natural water as in an artificial medium. The lytic activity of AC-1 was dependent on the photosynthetic activity of A. cylindrica. When observed under phase contrast microscope, the isolate lysed vegetative cells of A. cylindrica in scattered state in a liquid medium, whereas heterocysts have not been lysed.

Thermosensing of Thermotactic Mutants, Dictyostelium discoideum Amoebae in Vegetative Stage

  • Hong, Choo-Bong
    • Journal of Plant Biology
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    • v.26 no.3
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    • pp.131-139
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    • 1983
  • Temperature response of amoebae of thermotactic mutants have been investigated. Amoebae of the mutant strain HO 428 showed positive thermotaxs which is strong at lower temperaturs and drops sharply above the growth temperature of amoebae. The temperature response of HO 428 amoebae was not affected by the length of amoebae on the grdients. HO 596 amoebae seemed to have both positive and negative thermotactic responses shortly after food depletion. Longer exposure of these amoebae on the thermal gradients induced a stronger negative response at lower temperatures and an apparent positive response at higher temperatures. A similar changes could be observed in HO 1445 amoebae. Based on the steady positive thermotactic response by HO 428 amoebae and the mode of change in termperature response at higher temperatures, 24$^{\circ}C$ and $25^{\circ}C$, by HO 596 amoebae, a model for the temperature response of vegetative Dictyostelium discoideum amoebae, strain HL 50, has been proposed. The main features of the model are: a positive response at the thermal gradients with midpoint temperatures lower than the growth temperatures of amoebae and a negative response above it.

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Cryopreservation of winter vegetation buds of Betula platyphylla var. japonica in liquid nitrogen (자작나무 동아의 액체질소 내 초저온 보존)

  • 안영희
    • Korean Journal of Plant Resources
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    • v.15 no.2
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    • pp.89-95
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    • 2002
  • In woody plant germplasms, using prefrozen dormant buds for materials is one way to achieve successful cryopreservation. The protocol of cryopreservation for White birch (Betula platyphylla var. japonica) winter vegetative buds is the following. First, the branches of White birch were collected in January 20, when the vegetative buds were still in a state of quiescence. The winter buds with about 5㎜ of xylem tissue were removed from the branches. They were dehydrated to moisture contents about 44% by air dry treatment. The buds were prefrozen, with the temperature being decreased by 5∼-20$\^{C}$ and then transfered to the LN(liquid nitrogen) maintained below -l96$\^{C}$. After cryopreservation, the vegetative buds were rapidly thawed in a water bath at 40$\pm$5$\^{C}$. In this case, the cell survival rate of samples was about 86%. After sterilization, buds were then cultured on MS medium. These results demonstrate the feasibility for cryopreservation of winter vegetation buds of Betula platyphylla var. japonica.

A phylogenetic study of Geranium (Geraniaceae) on the vegetative characters (쥐손이풀속(쥐손이풀과)의 외부영양형질에 의한 계통분류학적 연구)

  • Park, Seon-Joo
    • Journal of Environmental Science International
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    • v.11 no.10
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    • pp.1001-1009
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    • 2002
  • Vegetative characters (e.g. habitat, root, ramification and habit of stem, phyllotaxy, leaf sape, shape of leaf margin, shape of division and lobe of division, number of division, thick and texture of leaf and shape of stipule) on 35 species of Asian Geranium were reviewed to discussion propriety of taxonomic character and evolutionary trends. Wilfordii group is characterized by three divided leaf, Farreri group was grown a high altitude, and Maculatum group is grouped by life cycle of anneal. Arrangement state of leaf is alternate type G, tripartitum, G. eriostemon, and G. erianthum is thought the more primitive than the other taxon, and regarded as more advanced group the sibiricum group and pseudosibiricum group which divided of leaf deeply. Shape of leaf, division degree of lobes, shape of leaf margin and number of division are considered a good identification characters because width of change are fixed between population.

Efficiency Evaluation of Vegetative Filter Strip for Non-point Source Pollutant at Dense Upland Areas - Focused on Non-point Source Management Area Mandae, Gaa, and Jaun Basins - (고랭지밭 밀집지역 초생대의 비점오염 저감 효율 평가 - 비점오염원 관리지역을 중심으로 (만대지구, 가아지구, 자운지구) -)

  • Jeong, Yeonji;Lee, Dongjun;Kang, Hyunwoo;Jang, Won Seok;Hong, Jiyoung;Lim, Kyoung Jae
    • Journal of The Korean Society of Agricultural Engineers
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    • v.64 no.4
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    • pp.1-10
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    • 2022
  • A vegetative filter strip (VFS) is one of the best management practices (BMPs) to reduce pollutant loads. This study aims to assess the effectiveness of VFS in dense upland field areas. The study areas are agricultural fields in the Maedae (MD), Gaa (GA), and Jaun (JU) watersheds, where severe sediment yields have occurred and the Korean government has designated them as non-point management regions. The agricultural fields were divided into three or four clusters for each watershed based on their slope, slope length, and area (e.g., MD1, MD2). To assess the sediment trapping (STE) and pesticide reduction efficiency (PRE) of VFS, the Vegetative Filter Strip Modeling System (VFSMOD) was applied with three different scenarios (SC) (SC1: VFS with rye vegetation; SC2: VFS with rye vegetation and a gentle slope in VFS range; and SC3: VFS with grass mixture). For SC1, there were relatively short slope lengths and small areas in the MD1 and GA3 clusters, and they showed higher pollutant reduction (STE>50%, PRE>25%). For SC2 and SC3, all clusters in GA and some clusters (MD1 and MD3) in MD show higher pollutant reduction (>25%), while the uplands in JU still show a lower pollutant (<25%). With correlation analysis between geographic characteristics and VFS effectiveness slope and slope length showed relative higher correlations with the pollutant efficiency than a area. The results of this study implied that slope and slope length should be considered to find suitable upland conditions for VFS installations.

Impact of Quarrying Activities on the Surrounding Vegetation in Ogun State, Nigeria

  • Isiaka Adio, Hassan
    • Journal of Forest and Environmental Science
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    • v.38 no.4
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    • pp.263-274
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    • 2022
  • Quarrying of rock for construction purposes is a significant industry in any economy but has its negative impact. This study examined the impact of quarry activities on surrounding vegetation in Ogun State. Geographic Information System approach was used to map the various quarry locations present in different Local Government Areas in Ogun State; of which eight sites were selected namely Isara, Idode, Iwaye, Ogbere, Ilagbe, Adelokun Baaki Ake and Igodo. Vegetation composition analyses were carried out on the eight sites using Haga Ultimeter and chlorophyll content analysis. Data were subjected to descriptive and inferential statistics using SAS package (9.4 version). Sixty quarries were identified with Odeda Local Government Area (38.3%) having the highest percentage of quarry. The vegetative compositions analyses showed that Albizia zygia had the highest frequency (7) among identified plants in the quarries. The chlorophyll content of Albizia zygia in the wet season (492.2 mg Chl/m2) was significantly higher than dry season (464.4 mg Chl/m2) in all locations. However, Baaki Ake (Albizia zygia) chlorophyll content was highest among other locations in both seasons. In conclusion Albizia zygia showed highest resistance to quarry activities, hence common among other plants identified around the quarries.

Evaluation of carbon flux in vegetative bay based on ecosystem production and CO2 exchange driven by coastal autotrophs

  • Kim, Ju-Hyoung;Kang, Eun Ju;Kim, Keunyong;Jeong, Hae Jin;Lee, Kitack;Edwards, Matthew S.;Park, Myung Gil;Lee, Byeong-Gweon;Kim, Kwang Young
    • ALGAE
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    • v.30 no.2
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    • pp.121-137
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    • 2015
  • Studies on carbon flux in the oceans have been highlighted in recent years due to increasing awareness about climate change, but the coastal ecosystem remains one of the unexplored fields in this regard. In this study, the dynamics of carbon flux in a vegetative coastal ecosystem were examined by an evaluation of net and gross ecosystem production (NEP and GEP) and $CO_2$ exchange rates (net ecosystem exchange, NEE). To estimate NEP and GEP, community production and respiration were measured along different habitat types (eelgrass and macroalgal beds, shallow and deep sedimentary, and deep rocky shore) at Gwangyang Bay, Korea from 20 June to 20 July 2007. Vegetative areas showed significantly higher ecosystem production than the other habitat types. Specifically, eelgrass beds had the highest daily GEP ($6.97{\pm}0.02g\;C\;m^{-2}\;d^{-1}$), with a large amount of biomass and high productivity of eelgrass, whereas the outer macroalgal vegetation had the lowest GEP ($0.97{\pm}0.04g\;C\;m^{-2}\;d^{-1}$). In addition, macroalgal vegetation showed the highest daily NEP ($3.31{\pm}0.45g\;C\;m^{-2}\;d^{-1}$) due to its highest P : R ratio (2.33). Furthermore, the eelgrass beds acted as a $CO_2$ sink through the air-seawater interface according to NEE data, with a carbon sink rate of $0.63mg\;C\;m^{-2}\;d^{-1}$. Overall, ecosystem production was found to be extremely high in the vegetated systems (eelgrass and macroalgal beds), which occupy a relatively small area compared to the unvegetated systems according to our conceptual diagram of a carbon-flux box model. These results indicate that the vegetative ecosystems showed significantly high capturing efficiency of inorganic carbon through coastal primary production.

Complications amd Mortality After Coronary Artery Bypass Graft Surgery; Collective Review of 61 Cases (관상동맥우회수술후 합병증과 사망율에 대한 임상적 고찰;61례 보고)

  • 조건현
    • Journal of Chest Surgery
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    • v.26 no.7
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    • pp.526-531
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    • 1993
  • Sixty-one consecutive patients with coronary artery bypass graft for myocardial revascularization were retrospectively reviewed to analyze various pattern of postoperative complication and death during hospital stay from Nov. 1988 to Oct. 1992. Fortytwo of the patients were male and nineteen female. The mean age was 56 and 51 years in male and female. Preoperative diagnosises were unstable angina in 14 of patients, stable angina in 28, postmyocardial infarction state in 15, and state of failed percutaneous transluminal coronary angioplasty in 4. 141 stenosed coronary arteries were bypassed with use of 20 pedicled internal mammary artery and 124 reversed saphenous vein grafts. Postoperative complications and perioperative death were as follows: 1. Of 61 patients undergoing operation, peri and postoperative over all complication occured in 15 patients [ 25% ]; newly developed myocardial infarction in 4, intractable cardiac arrhythmia including atrial fibrillation and frequent ventricular premature contraction in 3, bleeding from gastrointestinal tract in 2, persistent vegetative state as a sequele of brain hypoxia in 1, wound necrosis in 1, left hemidiaphragmatic palsy in 3 and poor blood flow through graft in 2. 2. Operative mortality was 8%[5 patients]. 3 out of these died in operating room; 1 patient by bleeding from rupture of calcified aortic wall, 1 by air embolism through left atrial vent catheter, 1 by low cardiac output syndrome. 2 patients died during hospital stay; 1 by acute respiratory distress syndrome with multiuple organ failure, 1 by brain death after delayed diagnosis of pericardial tamponade.

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Two groups of S-layer proteins, SLP1s and SLP2s, in Bacillus thuringiensis co-exist in the S-layer and in parasporal inclusions

  • Zhou, Zhou;Peng, Donghai;Zheng, Jinshui;Guo, Gang;Tian, Longjun;Yu, Ziniu;Sun, Ming
    • BMB Reports
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    • v.44 no.5
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    • pp.323-328
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    • 2011
  • We screened four B. thuringiensis strains whose parasporal inclusions contained the S-layer protein (SLP), and cloned two slp genes from each strain. Phylogenetic analysis indicated these SLPs could be divided into two groups, SLP1s and SLP2s. To confirm whether SLPs were present in the S-layer or as a parasporal inclusion, strains CTC and BMB1152 were chosen for further study. Western blots with isolated S-layer proteins from strains CTC and BMB1152 in the vegetative phase showed that SLP1s and SLP2s were constituents of the S-layer. Immunofluorescence utilizing spore-inclusion mixtures of strains CTC and BMB1152 in the sporulation phase showed that SLP1s and SLP2s were also constituents of parasporal inclusions. When heterogeneously expressed in the crystal negative strain BMB171, four SLPs from strains CTC and BMB1152 could also form parasporal inclusions. This temporal and spatial expression is not an occasional phenomenon but ubiquitous in B. thuringiensis strains.

Cloning and Characterization of a PI-like MADS-Box Gene in Phalaenopsis Orchid

  • Guo, Bin;Hexige, Saiyin;Zhang, Tian;Pittman, Jon K.;Chen, Donghong;Ming, Feng
    • BMB Reports
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    • v.40 no.6
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    • pp.845-852
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    • 2007
  • The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.