• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.6
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• pp.789-792
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• 2004

In various fields as web mining, bioinformatics, statistical data analysis, and so forth, very diversely missing values are found. These values make training data to be sparse. Largely, the missing values are replaced by predicted values using mean and mode. We can used the advanced missing value imputation methods as conditional mean, tree method, and Markov Chain Monte Carlo algorithm. But general imputation models have the property that their predictive accuracy is decreased according to increase the ratio of missing in training data. Moreover the number of available imputations is limited by increasing missing ratio. To settle this problem, we proposed statistical learning theory to preprocess for missing values. Our statistical learning theory is the support vector regression by Vapnik. The proposed method can be applied to sparsely training data. We verified the performance of our model using the data sets from UCI machine learning repository.

• The Journal of the Acoustical Society of Korea
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• v.23 no.5
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• pp.420-427
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• 2004

This paper presents real time implementation of 4kbps EHSX (Enhanced Harmonic Stochastic Excitation) speech coder that combines the harmonic vector excitation coding with time-separated transition coding. The harmonic vector excitation coding uses the harmonic excitation coding for voiced frames and used the vector excitation coding with the structure of analysis-by-synthesis for unvoiced frames, respectively. For transition frames mixed with voiced and unvoiced signal, we use the time-separated transition coding. In this paper. we present the optimization methods of implementation speech coder on the EMS320C6701/sup (R)/ DSP. To reduce the complex for real-time implementation. we perform the optimization method in algorithm by replacing the complex sinusoidal synthesis method with IFFT. and we apply fully pipelines hand assembly coding after converting it from floating source to fixed source. To generate a more efficient code. we also make use or the available EMS320C6701/sup (R)/ resources such as Fastest67x library and memory organization.

• The Journal of the Acoustical Society of Korea
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• v.35 no.5
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• pp.403-409
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• 2016

Traditional towed line arrays using omni-directional sensor suffer from the well known port-starboard ambiguity, because the direction of arrival is determined by conic angle. The operational method and structure of the sensor arrays method have been proposed to solve this problem. Recently, a lot of research relating to the acoustic vector sensor are studied. In this paper, we study port-starboard discrimination for roll of acoustic vector sensor array. With one omni-directional sensor and three orthogonally-placed directional sensors, an acoustic vector sensor is able to measure both the acoustic pressure and the three directional velocities at the point of the sensor. The wrong axis due to the roll at directional sensors can degrade performance of beamforming. We investigate port-starboard discrimination for roll of sensor array and confirm the validity of performance of beamforming with compensated the roll.

• The Journal of the Acoustical Society of Korea
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• v.19 no.3
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• pp.26-34
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• 2000

In this paper, we propose a 4kbps speech coder that combines the harmonic vector excitation coding with time-separated transition coding. The harmonic vector excitation coding uses the harmonic excitation coding in the voiced frame and uses the vector excitation coding with the structure of analysis-by-synthesis in the unvoiced frame, respectively. But two mode coding method is not effective for transition frame mixed in voiced and unvoiced signal and a new method beyond using unvoiced/voiced mode coding is needed. Thus, we designed a time-separated transition coding method for transition frame in which a voiced/unvoiced decision algorithm separates unvoiced and voiced duration in a frame, and harmonic-harmonic excitation coding and vector-harmonic excitation coding method is selectively used depending on the previous frame U/V decision. In the decoder, the voiced excitation signals are generated efficiently through the inverse FFT of harmonic magnitudes and the unvoiced excitation signals are made by the inverse vector quantization. The reconstructed speech signal are synthesized by the Overlap/Add method.

• BMB Reports
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• v.29 no.4
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• pp.359-364
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• 1996

By both in vitro hydroxylamine mutagenesis of the wild type 3-phosphoglycerate kinase gene (PGK) promoter DNA and insertion of the leu2-d gene, we have created yeast expression vectors potentially useful for production of eukaryotic genes in yeast. The guanine (G) to adenine (A) change at the -3 position from the ATG start codon of the PGK promoter-based vector rendered a 6~7 times elevated expression of the adjacent eukaryotic gene, and insertion of the leu2-d gene in the vector containing the mutated PGK promoter further enhanced the expression of the gene. When expression of the AIDS virus HIV1-gagP17 gene in a lacZ fusion form was examined with this new vector, a 15 times higher level of expression than that from the original PGK promoter was observed. Northern and Southern analysis showed that this elevated expression is due to the production of a high copy number of mRNA by leu2-d gene functioning and by efficient translation of the produced mRNA. Thus, the vector that contained the A at the -3 position from the ATG start codon in the promoter region and the leu2-d gene shows increased expression capability and will be potentially useful for production of eukaryotic genes in yeast.

• Journal of Aquaculture
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• v.11 no.4
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• pp.457-463
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• 1998

An expression vector, pUC19N6-luc, containing nuclear matrix attachment region(MAR) isolated from Misgurnus mizolepis liver and control expressino vector, pUC19-luc, were constructed. After these vectors were transferred into CHSE-214 cell line by electroporation, the expression rate of luckferase gens, copy number of vectors and chromosome integration of vectors were analyzed by using assay of luciferase activity, PCR and Southern blotting. While the expression pattern of luciferase gene of pUC19-luc was shown in typicla transient ecpression pattern, that of pUC19N6-luc was highly increased at the 5 days after transfectrion. Although the cope number of pUC19N6-luc vector was higher than that of pUC19-luc vector, these vectors were integrated into chromosome at the same time point in the transfected CHSE-214 cells. In conclusion, the increase of luciferase gene expression of pUC19N6-luc was resulted from not the maintaining of the high copy number but the formation of transcription-favorable structure by MAR effect after chromosomal integration.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.12
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• pp.5860-5876
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• 2018

A single snapshot data can only provide limited amount of information so that the rank of covariance matrix is not full, which is not adopted to complete the parameter estimation directly using the traditional super-resolution method. Aiming at solving the problem, a joint time delay and angle estimation using matrix pencil method based on information reconstruction vector for orthogonal frequency division multiplexing (OFDM) signal is proposed. Firstly, according to the channel frequency response vector of each array element, the algorithm reconstructs the vector data with delay and angle parameter information from both frequency and space dimensions. Then the enhanced data matrix for the extended array element is constructed, and the parameter vector of time delay and angle is estimated by the two-dimensional matrix pencil (2D MP) algorithm. Finally, the joint estimation of two-dimensional parameters is accomplished by the parameter pairing. The algorithm does not need a pseudo-spectral peak search, and the location of the target can be determined only by a single receiver, which can reduce the overhead of the positioning system. The theoretical analysis and simulation results show that the estimation accuracy of the proposed method in a single snapshot and low signal-to-noise ratio environment is much higher than that of Root Multiple Signal Classification algorithm (Root-MUSIC), and this method also achieves the higher estimation performance and efficiency with lower complexity cost compared to the one-dimensional matrix pencil algorithm.

• The Journal of the Convergence on Culture Technology
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• v.7 no.3
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• pp.591-598
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• 2021

It's possible to get the word-vector by the statistical SVD or deep-learning CBOW and LSTM methods and theses ones learn the contexts of forward/backward words or the sequence of following words. It's used to analyze the poems by Ki Hyung-do with similar words recommended by the word-vector showing the core images of the poetry. It seems at first sight that the words don't go well with the images but they express the similar style described by the reference words once you look close the contexts of the specific poems. The word-vector can analogize the words having the same relations with the ones between the representative words for the core images of the poems. Therefore you can analyze the poems in depth and in variety with the similarity and analogy operations by the word-vector estimated with the statistical SVD or deep-learning CBOW and LSTM methods.

• Journal of Microbiology and Biotechnology
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• v.32 no.3
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• pp.278-286
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• 2022

Live bacterial vector vaccines are one of the most promising vaccine types and have the advantages of low cost, flexibility, and good safety. Meanwhile, protein secretion systems have been reported as useful tools to facilitate the release of heterologous antigen proteins from bacterial vectors. The twin-arginine translocation (Tat) system is an important protein export system that transports fully folded proteins in a signal peptide-dependent manner. In this study, we constructed a live vector vaccine using an engineered commensal Escherichia coli strain in which amiA and amiC genes were deleted, resulting in a leaky outer membrane that allows the release of periplasmic proteins to the extracellular environment. The protective antigen proteins SLY, enolase, and Sbp against Streptococcus suis were targeted to the Tat pathway by fusing a Tat signal peptide. Our results showed that by exploiting the Tat pathway and the outer membrane-defective E. coli strain, the antigen proteins were successfully secreted. The strains secreting the antigen proteins were used to vaccinate mice. After S. suis challenge, the vaccinated group showed significantly higher survival and milder clinical symptoms compared with the vector group. Further analysis showed that the mice in the vaccinated group had lower burdens of bacteria load and slighter pathological changes. Our study reports a novel live bacterial vector vaccine that uses the Tat system and provides a new alternative for developing S. suis vaccine.

• Journal of the Korean Magnetics Society
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• v.7 no.2
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• pp.109-116
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• 1997

This paper describes an implementation of the rotational hysteresis analysis using the finite element method and a vector hysteresis model. The vector hysteresis model is extended from the magnetization-dependent Preisach model. The magnetization of each element is calculated for a given history of the rotating field. The lagging angle and the magnitude of the magnetization for an applied field are evaluated. The experimental results show that the magnetization characteristics of the magnetic materials under the rotating field can be analyzed accurately by the proposed method.