• Journal of Aquaculture
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• v.13 no.1
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• pp.29-37
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• 2000

In previously study we isolated several fish matrix attachment regions (MARs) capable of replicating the plasmid by itself. In this study we construct a fish expression vector pBaEGFP(+) containing mud loach ${\beta}$-actin promoter EGFP as reporter gene and SV40 signal. To analyze the effects of the fish expression vector respectively. The fish ARS containing constructs pBaEGFP(+)-ARSs were transfected cells with pBaEGFP(+)-ARS101 and pBaEGFP(+)-ARS223 reduced 10 days to 25 days and then was constant to 30 days after transfection while that of the control vector without ARS element was basal level. The intensity of both constructs showed about 30fold of the intensity compared with the control vector on 30days after transfection individually .E. coli back-transformation analysis shows that pBaEGFP(+)-ARS223 and pBaEGFP(+)-ARS905 maintain in episomal state at least 30 days after transfection. The result indicates that both may be able to replicate the vector in BF-2 cell. Therefore the matrix-attached ARSs enhancing expression of the reporter gene might be useful as a component o the expression vector for transgenic studies.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.12
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• pp.2937-2943
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• 2013

This paper introduces a real-time flocking simulation framework through radial basis function(RBF). The proposed framework first divides the entire environment into a grid structure and then assign a vector per each cell. These vectors are automatically calculated by using RBF function, which is parameterized from user-input control lines. Once the construction of vector field is done, then, flocks determine their path by following the vector field flow. The collision with static obstacles are modeled as a repulsive vector field, which is ultimately over-layed on the existing vector field and the inter-individual collision is also handled through fast lattice-bin method.

• Proceedings of the KIPE Conference
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• 1999.07a
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• pp.333-337
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• 1999

This paper presents the DQ transfomation space space vector modulation method to devel control algorithm of distribu STATCON(STATic CONdenser) for line volt regulation, dc link voltage regulation harmonics compensation. The Performance an of a PI with ramp comparision and synchro reference frame current controller is carried Based on these analysis, the control perform are desirable to compensate the harmonics a regulate dc link and line voltage of Distrib line.

• Proceedings of the KIEE Conference
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• 2002.04a
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• pp.15-17
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• 2002

In this paper, we analyse the dynamic characteristic of 3-phase line start permanent magnet motor considering magnetization. Magnetization vector of NdFeB is obtained from the 2-D FEM magnetization analysis. And comparing the proposed analysis with conventional analysis method, we know that it is necessary to consider magnetization in dynamic analysis.

• Korean Journal of Animal Reproduction
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• v.19 no.1
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• pp.35-41
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• 1995

In this study, we have tested whether the retrovirus vector system is applicable in transgenic cattle production. To overcome low infectivity of currently available retrovirus vector system we have directly microinjected retrovirus-producing cells into the perivitelline space of the day 1.5 embryos. The virus-producing cell line was designed to release replication-defective retrovirus encapsidated with Gibbon ape leukemia virus (GaLV) envelope protein. E. coli LacZ gene was used as a marker gene to facilitate evaluation of the transgene expression and X-gal staining at morula or blastocyst stage resulted in expression of E. coli LacZ gene The results in these experiments were summarized as follows : 1. The lowest concentration of polybrene necessary for efficient virus infection was Sf' g/ml. 2. Development rate from day 1.5 embryos microinjected with virus-producing cells to the morulae /blastocysts was 29%. 3. 21% of the morulae /blastocysts were LacZ+. 4. There was no evidence that the retrovirus-producing cells used in this study produced replication-competent retrovirus.

• Progress in Medical Physics
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• v.18 no.4
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• pp.214-220
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• 2007

Diffusion tensor tractorgraphy which is based on line propagation method with brute force approach is implemented and the vector correlation function is proposed in addition to the conventional fractional anisotrophy value as a criterion to select seed points. For the whole tractography, the proposed method used 41 % less seed points than the conventional brute force approach for $FA{\geq}0.3$ and most of the fiber tracks in the outer region of white matter were removed. For the corticospinal tract passing through region of interest, the proposed method has produced similar results with 50% less seed points than conventional one.

• IE interfaces
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• v.16 no.4
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• pp.450-462
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• 2003

In this paper, we deal with a process and layout design for producing a planned amount of Sikhye in a given limited time period under a reasonable production schedule. We represent a Sikhye production line as a vector N, the element of which denotes the number of tanks required in each process and our objective is to find an appropriate vector which minimizes the total investment cost. We suggest a systematic method for finding an appropriate N and an appropriate layout to N. In detail, first, we decide the required sequence of processes and the required operations for each process and we estimate standard operating times. Second, constructing a precedence diagram, we find a critical path in order to reduce the total production lead time for a batch of Sikhye. Third, given a limited N space, we manage to construct manually each production schedule using both the processing times of the critical operations and transfer times. Finally, we find an optimal vector N which gives a minimum investment cost and meets both the time constraint and quantity constraint. In addition, with the estimated relative size of each tank, we suggest an appropriate conceptual layout design including an expansion area for future demands, based on the span technique used in the field of architectural design.

• Journal of KIISE:Computing Practices and Letters
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• v.14 no.8
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• pp.763-767
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• 2008

In this paper, to make a high performance and low power CORDIC architecture for 3D operations in mobile devices, we suggest two off-line vectoring algorithms named Angle Based Search (ABS) and Scaling Considered Search (SCS). The ABS algorithm represents a 3D vector with two angles and those angles are used as a condition for searching CORDIC rotation sequences. The SCS algorithm determines the best CORDIC rotation sequence in advance to eliminate extra scaling computation. Using the proposed algorithms, we can observe 50% of latency is reduced. Furthermore, we perform a simple analysis and discuss possible reduction of power consumption by applying voltage scaling method together with the proposed algorithm.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.14 no.7
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• pp.734-740
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• 2003

In this paper, A Vector Modulator based a wideband analog phase shifter is realized with four P-I-N diode attenuators, an asymmetric coupled line coupler, a symmetric coupled line coupler, and a power combiner. Simple configuration to have advantages in cost, size, power, and the number of passive circuits is presented. The phase variation due to phase and amplitude error of a P-I-N diode attenuator is derived and used to optimize the overall circuit. The phase shifter shows a total phase shift of 360$^{\circ}$, a 8.2$^{\circ}$maximum phase error, and a 16${\pm}$2.5 dB insertion loss over the wide frequency range of 1 GHz to 3 GHz.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.544-550
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• 2005

To develop a highly efficient mammalian expression vector, a series of vectors were constructed based on the murine cytomegalovirus (MCMV) immediate-early (IE) promoter and human ${\beta}$-globin second intron. The resulting MCMV promoter was several-fold stronger than the HCMV promoter in various mammalian cell lines, such as the NIH3T3, Neuro-2a, 293T, and HT1080 cell lines, and was only slightly weaker than the HCMV promoter in HeLa and CHO cells. The inclusion of the human ${\beta}$-globin second intron behind the MCMV promoter or HCMV promoter markedly enhanced the promoter activity in various mammalian cell lines, and the resultant MCMV/Glo-I expression system was stronger than the HCMV promoter from 4.7- to 11.2-fold in every cell line tested. Also, the MCMV/Glo-I promoter induced a higher level of the VSV-G protein in a transiently transfected 293T cell line, which is useful for the production of recombinant retrovirus and lentivirus vectors.