• 제목/요약/키워드: Vector Generation

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Generation of Baculovirus Expression Vector Using Detective Autographa California Nuclear Polyhedrosis Virus Genome Maintained in Escherichia coli for $Occ^{+}$ Virus Production

  • Je, Yeon-Ho;Chang, Jin-Hee;Roh, Jong-Yul;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.155-160
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    • 2001
  • We have generated a novel baculovirus genome which can be maintained in Escherichia coli that facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. To make $Occ^{+}$ recombinant expression vectors, polyhedrin gene under the control of p10 promoter was inserted to bAcGOZA and this genome was designated bApGOZA. As in bAcGOZA, bApGOZA lacks a portion of the essential ORF1629 gene, but includes a mini-F replicon and selectable kanamycin-resistance marker, This occasion-producing activity of bApGOZA can be used very conveniently for its oral infectivity to insect larvae in mass production of foreign protein and insecticides.

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The Improvement of Continuation Power Flow System Including the Algorithm of Practical Step Length Selection (실용적인 스텝크기 선택 알고리듬을 고려한 연속조류계산 시스템의 개발)

  • Song, Hwa-Chang;Lee, Byong-Jun;Kwon, Se-Hyuk
    • The Transactions of the Korean Institute of Electrical Engineers A
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    • v.48 no.3
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    • pp.190-196
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    • 1999
  • Continuation power flow has been developed to remove the ill-condition problem caused by singularity of power flow Jacobian at and near at steady-state voltage instability point in conventional power flow. Continuation power flow consists of predictor and corrector. In prddictor, the direction vector at the resent solution is caluculated and the initial guess of next solution is determined at the distance of step length. The selection of step length is a very important part, since computational speed and convergence performance are both greatly affected by the choice of the step length. This paper presents the practical step length selection algorithm using the reactive power generation sensitivith. In numulation, the proposed algorithm is compared with step length selection algorithm using TVI(tangent vector index).

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Analogical Face Generation based on Feature Points

  • Yoon, Andy Kyung-yong;Park, Ki-cheul;Oh, Duck-kyo;Cho, Hye-young;Jang, Jung-hyuk
    • Journal of Multimedia Information System
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    • v.6 no.1
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    • pp.15-22
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    • 2019
  • There are many ways to perform face recognition. The first step of face recognition is the face detection step. If the face is not found in the first step, the face recognition fails. Face detection research has many difficulties because it can be varied according to face size change, left and right rotation and up and down rotation, side face and front face, facial expression, and light condition. In this study, facial features are extracted and the extracted features are geometrically reconstructed in order to improve face recognition rate in extracted face region. Also, it is aimed to adjust face angle using reconstructed facial feature vector, and to improve recognition rate for each face angle. In the recognition attempt using the result after the geometric reconstruction, both the up and down and the left and right facial angles have improved recognition performance.

A Study on the Generation of Initial Shape for the Initiation of Optimal Blank Design Sequence (최적블랭크 설계를 위한 초기형상 생성에 관한 연구)

  • 심현보;장상득;박종규
    • Transactions of Materials Processing
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    • v.13 no.1
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    • pp.90-101
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    • 2004
  • An inverse mosaic method has been proposed to generate an initial blank shape from the final product shape. Differently from the geometric mapping method, the method can handle triangular patches. However, the generated blank shape is strongly dependent on the order of determination of nodes. In order to compensate the dependency error smoothing technique has been also developed. Although the accuracy has been improved greatly compared with the geometrical mapping method, the method has limitation, due to the no incorporation of plasticity theory. Even though the accuracy of the radius vector method is already proved. the method requires initial guess to start the method. In order to compromise the limitation of the present method and the radius vector method, the method has been connected to the radius vector method. The efficiency of the present optimal blank design method has been verified with some chosen examples.

Wavelet Image Data Compression Using Vector Quantization (벡터양자화를 이용한 웨이브렛 영상데이터 압축)

  • 최유일;조창호;이상효;조도현;이종용
    • Proceedings of the IEEK Conference
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    • 2003.07e
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    • pp.2287-2290
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    • 2003
  • In this paper, an image vector quantization method is proposed not only to improve the compression ratio but also to reduce the computation cost. The proposed method could save the computation cost of codebook generation and encoding by using the modified LBG algorithm of Partial Search Partial Distortion (PSPD) in wavelet domain, by which the code book was constructed together with the partial codebook search, the partial code vector elements, and the interruption criterion. We have designed and implemented the vector quantizer to verify the improvement in reducing compression ratio in encoding processing and reducing the computation cost.

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Envelope Generation for Freeform Objects (자유 곡면체의 엔벨롭 생성)

  • 송수창;김재정
    • Korean Journal of Computational Design and Engineering
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    • v.6 no.2
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    • pp.89-100
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    • 2001
  • Swept volume is the sweeping region of moving objects. It is used in various applications such as interference detection in assembly design, visualization of manipulator motions in robotics, simulation of the volume removal by a cutter in NC machining. The shape of swept volume is defined by the envelope, which is determined by the boundary of moving objects and its direction of motion. In order to implement the generation of swept volume, researchers have taken much effort to develop the techniques how to generate the envelope. However, their results are confined to envelope generated only in simple shape objects, such as polyhedra or quadric surfaces. This study provided the envelope generation algorithm of NURBS objects. Characteristic points were obtained by applying the geometric conditions of envelope to NURBS equations, and then characteristic curves were created by means of interpolating those points. Silhouette edges were determined in the following procedures. First, two adjacent surfaces which have the same edge were found from B-Rep data. Then, by taking the scalar product of velocity vector of a point on that edge with each normal vector on two surfaces, silhouette edges were discriminated. Finally, envelope was generated along moving direction in the form of ruled surfaces by using both the partial information between initial and final position of objects affecting envelope along with characteristic curves and silhouette edge. Since this developed algorithm can be applied not only to NURBS objects but also to their Boolean objects, it can be used effectively in various applications.

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Design of the Current and Speed Controller for the IPMSM based High Speed Railway Traction System (IPMSM이 적용된 차세대 고속철도 견인시스템의 전류 및 속도 제어기 설계)

  • Yi, Du-Hee;Jin, Kang-Hwan;Kwon, Soon-Hwan;Kim, Sung-Je;Kim, Yoon-Ho
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.24 no.8
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    • pp.70-77
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    • 2010
  • This paper presents the current and speed controller design procedure and their performance for the IPMSM based next generation high speed railway traction system. The next generation high speed railway system is a power distributed type and uses vector control method for a motor speed control. Since the speed and current controller gains of the vector control system directly affects to the transient characteristics and speed control capability, the systematic design of the controllers are required. In this paper the controllers are designed using the IPMSM based next generation high speed railway system parameters. Simulation programs based on Matlab/Simulink is developed. Finally the controller characteristics are analyzed by the simulation results.

A Study on the Pedestrian Detection on the Road Using Machine Vision (머신비전을 이용한 도로상의 보행자 검출에 관한 연구)

  • Lee, Byung-Ryong;Truong, Quoc Bao;Kim, Hyoung-Seok;Bae, Yong-Hwan
    • Journal of Institute of Control, Robotics and Systems
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    • v.17 no.5
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    • pp.490-498
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    • 2011
  • In this paper, we present a two-stage vision-based approach to detect multi views of pedestrian in road scene images. The first stage is HG (Hypothesis Generation), in which potential pedestrian are hypothesized. During the hypothesis generation step, we use a vertical, horizontal edge map, and different colors between road background and pedestrian's clothes to determine the leg position of pedestrian, then a novel symmetry peaks processing is performed to define how many pedestrians is covered in one potential candidate region. Finally, the real candidate region where pedestrian exists will be constructed. The second stage is HV (Hypothesis Verification). In this stage, all hypotheses are verified by Support Vector Machine for classification, which is robust for multi views of pedestrian detection and recognition problems.

Cytotoxic Effects of an Oncolytic Adenoviral Vector AdLPCDIRESE1A in Hepatocellular Carcinoma Cells (암세포 용해성 AdLPCDIRESE1A 벡터의 간암 세포독성효과)

  • Chung, In-Jae
    • YAKHAK HOEJI
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    • v.55 no.1
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    • pp.75-79
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    • 2011
  • The replication competent adenoviral vector (AV), AdLPCDIRESE1A was generated and reported previously to have cytotoxic effects in some cell lines. In AdLPCDIRESE1A, the expression of cytosine deaminse (CD) and E1A genes are under the control of tumor-specific L-plastin promoter. CD enzyme can deaminate the nontoxic prodrug 5-fluorocytosine (5-FC) to the toxic 5-fluorouracil (5-FU). E1A gene is essential for viral replication. Primary liver cancer, most of which is hepatocellular carcinoma (HCC), is the third common leading cancer in Korea. Thus, we have conducted in vitro preclinical study to evaluate effectiveness of AdLPCDIRESE1A on HCC. The efficacy of cytotoxicity was measured by generation of cytopathic effect (CPE) and cell counting. We infected HepG2 cells with various MOI of vector alone or concurrent with 5-FC. Exposure of cells to AdLPCDIRESE1A generated a significant cytotoxic effect as compared to the control. Almost 83% of the cell had manifested the characteristic cytotoxic effect on day 9 after infection of cells with 10 MOI of vector. We also observed the additive cytotoxic effects when AdLPCDIRESE1A vector had been coadministrated with 5-FC. The results suggest that the use of AdLPCDIRESE1A/5FC may be value in treatment of liver cancer. Further animal studies are needed for clinical trial.

Recombinant Adenoviral Vector Containing Tumor-Specific L-Plastin Promoter Fused to Cytosine Deaminase Gene as a Transcription Unit: Generation and Functional Test

  • Chung, In-Jae;Deisseroth, Albert-B.
    • Archives of Pharmacal Research
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    • v.27 no.6
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    • pp.633-639
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    • 2004
  • The expression of therapeutic transgenes in recombinant adenoviral vectors is a major cause of toxicity in dividing cancer cells as well as non dividing normal cells. To solve the problem of toxicity to normal cells, we have reported on a recombinant adenoviral vector system (AdLP-) in which the expression of the transgene is directed by the tumor-specific L-plastin promoter (LP) (Chung et al., 1999). The object of this study was to generate a recombinant adenoviral vector system which would generate tumor cell specific expression of cytosine deaminase (CD) gene. We report the construction of a replication-incompetent adenoviral vector in which CD is driven by the L-plastin promoter (AdLPCD). Infection of 293 cells by AdLPCD generated the functional CD protein as measured by HPLC analysis for the conversion of 5-Fluorocy-tosine (5-FC) to 5-Fluorouracil (5-FU). HPLC analysis in conjunction with counting radioactivity for [6-$^3$H]-5FC and [6-$^3$H]-5FU demonstrated vector dose-dependent conversion of 5-FC to 5-FU in AdLPCD infected ovarian cancer cells. The results from present and previous studies(Peng et al., 2001; Akbulut et al., 2003) suggest that the use of the AdLPCD/5-FC system may be of value in the treatment of cancer including microscopic ovarian cancer in the peritoneal cavity.