• Journal of agricultural medicine and community health
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• v.5 no.1
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• pp.5-15
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• 1980

The first recorded trypanosomiasis epidemic in Uganda took place at the beginning of this century in the islands and in a strip along the northern shores of Lake Victoria, which resulted in deaths of 1/3 million people. The disease was partly controlled by early 1930's and continued to occur sporadically in certain localized foci. The disease has however flared up in an explosive outbreak in Busoga district along Lake Victoria since 1977. The incidence of disease in northern district adjacent to Southern Sudan is also increasing lately. This paper describes the three month observation on the surveillance and control activities in the epidemic areas and of various health units including the Vector Control Division, the Tsetse fly Control Division, Tororo Trypanosomiasis Research Institute, medical units in Busoga, and Acholi districts. Data analysis and review were made of disease information so far collected by various health units in the Ministry of Health and district health offices. The findings may be summarized in the following: 1) A total of 12, 100 patients and 38 deaths: have occured in Busoga district since 1977 onward, and over 100 cases of diseases arc occuring in the Northern region bordering Southern Sudan. 2) the distribution of trypanosomiasis is characterized with two district patterns. The disease caused by Trypanosoma rhodesiense occurs in Busoga and is transmitted by Glossina palpalis, G. fuscipes infested in the islands and in the northern shore of forests of Lake Victoria. Another type caused by Trypanosoma gambiense occurs in Madi and Acholi in the north and is transmitted by Glossina morsitans in Savannah. 3) The house survey in Rusoga indicated that most of patients keep domestic animals in their house premises, and are engaging in either farming or fishing. Practically all the patients remembered that they had been bitten by tsetse in the field. 4) The routine diagnostic methods in the hospital laboratory is carried out through the microscopic examination of trypanosome with Giemsa stain of blood and cerebro-spinal fluid, The measurement of ESR and IgM has been used by Tororo Tryponosomiasis Research Institute for field screening.

• Journal of the Korean Association of Geographic Information Studies
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• v.9 no.2
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• pp.227-238
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• 2006

Line density index(LDI) was developed to quantify a densely isothermal line rate as standard index in the ocean environment. Theoretical background on the LDI development process restricting index range 0 to 100 was described. And validation test was done for the LDI application condition that total line length is not greater than 1/10 of unit area. NOAA SST(Sea Surface Temperature) data were used for the experimental application of LDI in the South Sea of Korea. Using GIS, $0.1^{\circ}C$ isothermal lines were linearized as vector data form SST raster data, and unit area were built as polygon data. For the LDI calculation, spatial overlapping(line in polygon) was implemented. To analyze the effect of unit area size for the LDI distribution, two cases of unit area size were designed and descriptive statistics was calculated including performing normality test. The results showed no change of LDI's essential characteristics such as mean and normality except for the range of value, variance and standard deviation. Accordingly, it was found that complex structure of thermal front and even smaller scale of front width than unit area size could influence on the LDI distribution. Also, correlation analysis performed between LDI and difference of temperature(${\Delta}T^{\circ}C$), and horizontal thermal gradient(${\Delta}T^{\circ}C/km$) on the front was obtained from linear regression model. This obtained value was compared with the results from previous researches. Newly developed LDI can be used to compare the thermal front regions changing spatio-temporally in the ocean environment using absolute index value. It is considered to be significant to analyze the relationship between thermal front and marine environment or front and marine organisms in a quantitative approach described in this study.

• Journal of the Institute of Electronics and Information Engineers
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• v.51 no.5
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• pp.88-96
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• 2014

In modern electronic warfare systems, the necessity of a more accurate estimation method based on non-AOA (arrival of angle) measurement, such as TDOA and FDOA, have been increased. The previous researches using single TDOA have been carried out in terms of not only the development of emitter location algorithms but also the enhancement of measurement accuracy. Recently, however, the combined TDOA/FDOA method is of considerable interest because it is able to estimate the velocity vector of a moving emitter and acquire a pair of TDOA and FDOA measurements from a single sensor pair. In this circumstance, it is needed to derive the required FDOA measurement accuracy in order that the TDOA/FDOA combined localization system outperforms the previous single TDOA localization systems. Therefore, we analyze the contribution of FDOA measurement accuracy to emitter location, then propose the criterion based on CRLB (Cramer-Rao lower bound). Simulations are included to examine the validity of the proposed criterion by using the Gauss-Newton algorithm.

• Korean Chemical Engineering Research
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• v.55 no.3
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• pp.369-378
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• 2017

Vascular endotherial growth factor (VEGF) is a potent mitogen that stimulates vascular permeability and angiogenesis and has a potential in therapeutic applications. An industrial production method that provides high yield as well as purity is needed. Researches for various factors of mild solubilization with combination of ubiquitin fusion protein to increase solubility were carried out as well as by changing pH and denaturant concentration. Usage of pET28-a bacteral expression vector in BL21 (DE3) host cell was capable of producing approximately 14 g/L VEGF fusion protein in 20L fermentor. A purification process consisting of four chromatography steps including refolding and digestion with UBP1 resulted in mild solublization under the conditions of 2M urea and pH 10.0 due to ubiquitin fusion tag protein that increases in solubility of target protein VEGF. High yield of refolding and dimerization could be obtained between two step Ni-affinity chromatography. Multimeric and misfolded proteins and endotoxin were removed by DEAE anion exchange chromatography. Final monomers were removed from dimers by gel filtration chromatography. Characterization analysis of purified dimeric VEGF was performed using SDS-PAGE and RP-HPLC with a purity of 97%.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.41 no.1
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• pp.13-20
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• 2004

In this paper, we have analyzed the current MPEG-4 video encoding tools and proposed efcient coding techniques that reduce the complexity of the encoder. Until recently, encoder optimization without shape coding has been a major concern in video for wire/wireless low bit rate coding services. Recently, we found out that the computational complexity of MPEG-4 shape coding plays a very important role in the object-based coding through experiments. We have made an experiment whether we could get optimized object-based coding method through successfully combining latest optimized texture coding techniques with our proposed optimized shape coding techniques. In texture coding, we applied the MVFAST method for motion estimation. We chose not to use IVOPF(Intelligent VOP Formation) but to use TRB(Tightest Rectangular Boundary) for positioning VOP and, finally, to eliminate the spiral search of shape motion estimation to reduce the complexity in shape coding. As a result of experiment, our proposed scheme achieved improved time complexity over the existing reference software by $57.3\%$ and over the optimized method on which only shape coding was applied by $48.7\%$, respectively.

• Spatial Information Research
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• v.21 no.3
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• pp.89-101
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• 2013

Recently, importance of indoor space is on the rise, as larger and more complex buildings are taking place due to development of building technology. Accordingly, range of the target area of spatial information service is rapidly expanding from outdoor space to indoor space. Various demands for indoor spatial information are expected to be created in the future through development of high technologies such as IT Mobile and convergence with various area. Thus this research takes a look at available methods for building indoor spatial information and then builds high accuracy three-dimensional indoor spatial information using indoor high accuracy laser survey and 3D vector process technique. The accuracy of built 3D indoor model is evaluated by overlap analysis method refer to a digital map, and the result showed that it could guarantee its positional accuracy within 0.04m on the x-axis, 0.06m on the y-axis. This result could be used as a fundamental data for building indoor spatial data and for integrated use of indoor and outdoor spatial information.

• Biomedical Science Letters
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• v.2 no.2
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• pp.175-185
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• 1996

The mitogen-activated protein(MAP) kinase signal transduction pathway represents an important mechanism by which mitogen, such as serum and PMA, regulate cell proliferation and differentiation. Target substrates of the MAP kinase are located within several compartments containing plasma membranes and nucleus. We now report that serum addition induces proliferation of the P388 murine leukemia cell, but PMA does not, while both serum and PMA treatment cause translocation of the MAP kinase, mainly p42$^{mapk}$ isoform, from cytosol into the nucleus, which was monitored by immunoblot analysis using polyclonal anti-ERK1 antibodies. We investigated whether the MAP kinase was capable of phosphorylating c-Jun protein and GST-fusion proteins, the P562$^{kk}$N-terminal peptides (1-77 or 1-123 domain) of the T cell tyrosine kinase, using the partially purified MAP kinase by SP-sephadex C-50, phenyl superose and Mono Q column chromatography. We found that the partially purified MAP kinase was able to phosphorylate c-Jun protein and the GST-fusion protein expressed using E.coli DH5$\alpha$ which is transformed with pGEX-3Xb plasmid vector carrying of p562$^{kk}$N-terminal peptide-encoding DNA. These results imply that tyrosine kinase receptor/Ras/Raf/MAP kinase pathway is a major mechanism for mitogen-induced cell proliferation in P388 murine leukemia cell and that the various MAP kinase isoforms may have their own target substrates located in distinct subcellular compartments.

• BMB Reports
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• v.39 no.5
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• pp.642-647
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• 2006

Botulinum neurotoxin A (BoNT/A) has been used therapeutically to treat muscular hypercontractions and sudomotor hyperactivity and it has been reported that BoNT/A might have analgesic properties in headache. PEP-1 peptide is a known carrier peptide that delivers fulll-ength native proteins in vitro and in vivo. In this study, a BoNT/A gene were fused with PEP-1 peptide in a bacterial expression vector to produce a genetic in-frame PEP-1-BoNT/A fusion protein. The expressed and purified PEP-1-BoNT/A fusion proteins were efficiently transduced into cells in a time- and dose-dependent manner when added exogenously in a culture medium. In addition, immuno-histochemical analysis revealed that PEP-1-BoNT/A fusion protein efficiently penetrated into the epidermis as well as the dermis of the subcutaneous layer, when sprayed on mice skin. These results suggest that PEP-1-BoNT/A fusion protein provide an efficient strategy for therapeutic delivery in various human diseases related to this protein.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2001.11a
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• pp.102-102
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• 2001

Taurine (2-ethaneaminosulfonic acid) is one of the major intracellular ${\beta}$ -amino acids in mammals and is required for a number of biological processes including membrane stabilization, osmoregulation, antioxidation, detoxification, modulation of calcium flux and neurornodulation. The taurine transporter (TAUT) which contains 12 hydrophobic membrane-spanning domains has been cloned from dog kidney, rat brain, mouse brain, human thyroid, placenta and retina. In this study, The TAUT cDNA from the human intestinal epithelial cell, HT-29 was cloned and sequenced. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to amplify partial cDNA encoding human intestinal TAUT. The coding region of the PCR product was 732 bp long. The primers were designed to encode highly conserved amino acid sequences near the transmembrane domains III (IPYFIFLF) and Ⅵ (KYKYNSYR) both in human and mouse. The TAUT cDNA amplified was ligated into the pGEX 4T-1 expression vector. The resulting sequence of human intestinal TAUT cDNA (Accession number of NCBI Genebank is AF346763) was identical to the sequences of the TAUTs previously determined in the human placenta and retina except 3 base pairs from that of the reported human thyroid. TAUT specific antibodies were generated to use them as biological tools in the studies of the biological role of TAUT. Peptides of 149-162 amino acid residue (14 amino acids) of the TAUT were synthesized. The synthetic peptide used in this study was LFQSFQKELPWAHC. This region was chosen not only to avoid putative glycosylation sites but also to exclude regions of known homology with GABA transporters in the extracellular hydrophilic domains. The synthetic peptide, TAUT-1 was conjugated with carrier protein, kehole lympet hemocyanin (KLH) to use as an antigen. When used for immunization on a rabbit to produce polyclonal antiserum, the conjugates elicited high -titered specific anti-TAUT-1 antibodies, which reacted well with the ovalbumin (OVA) conjugated peptides in ELISA. The KLH-conjugated peptide was also used as immunizing antigen in BALB/c mice to produce TAUT specific monoclonal antibodies. From the culture supernatant of the hybridoma, the specificity of anti-TAUT-1 monoclonal antibodies was confirmed by ELISA. Further applications of more tools in TAUT expression analysis will be performed such as western blotting and flow cytometry.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.75-80
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• 2009

In order to develop transgenic sweetpotato plants [Ipomoea batatas (L.) Lam. cv. Yulmi] with enhanced tolerance to oxidative stress, we constructed transformation vectors expressing 2-Cys peroxiredoxin (Prx) gene under the control of the stress-inducible SWPA2 or enhanced 35S promoter (named as SP or EP). Transgenic sweetpotato plants were attempted to generate from embryogenic calli using an Agrobacterium-mediated transformation system. Embryogenic calli gave rise to somatic embryos and then converted into plantlets on MS medium containing 100 mg/L kanamycin. Transgenic plants were regenerated in the same medium. Southern blot analysis confirmed that the Prx gene was inserted into the genome of the plants. To further study we selected the transgenic plant lines with enhanced tolerance against methyl viologen (MV). When sweetpotato leaf discs were subjected to methyl MV at $20{\mu}M$, transgenic plants showed about 40% higher tolerance than non-transgenic or empty vector-transformed plants.