• Parasites, Hosts and Diseases
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• v.28 no.1
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• pp.45-52
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• 1990

Intestinal histopathological changes due to infection with Echinostcma hortense (Trematoda) were studied in rats after experimental infection with the metacercariae. The metacercariae were obtained from the tadpoles of Rana nigrcmaculata, a second intermediate host infected in the laboratory. Total 18 albino rats(Sprague-Dawley) were given 200 matacercariae each and sacrificed on the day 1, 3, 7, 11, 22 or 44 post-infection(PI) Segments of- the small intestine at 1, 3, 5, 8 and 30 cm posterior to the pylorus(PTP) were rejected and studied histopathologically. 1. The flukes were seen to have intruded into the intervillous space in the upper small intestine at early stages(1∼3 days PI), however, they were located mainly in the intestinal lumen at later stages(7∼44 days PI) . The flukes were sucking and destroying the epithelial layers of villi with their oral and ventral suckers. 2. Histopathological changes of the intestine were recognizable in as early as 1∼3 days after infection, and the changes became severer as the infection progressed. 3. The intestinal mucosa was histopathologically characterized by villous atrophy and crypt hyperplasia throughout the infection period. Major villous changes were blunting, fusion, severe destruction and loss of epithelial layers of villi. Villous/crypt(V/C) height ratio was remarkably reduced from 3 : 1 in controls to 1 : 1 in severely infected animals. In the stroma of villi, inaamma- tory cell infiltrations, vascular congestion, edema, and/or fibrosis were recognized. The goblet cells were increased in number after 11 days PI. It was revealed in the present study that the pathological changes in the intestine of rats infected with E. hortense were chieay confined to the mucosal layer of the upper small intestine, however, the changes were very severe accompanying remarkable destruction of villi and loss of mucosal integrity, and persistent until 44 days PI.

• Korean Journal of Plant Taxonomy
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• v.37 no.2
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• pp.155-196
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• 2007

This study was carried out to elucidate floristic data of Chirisan National Park by performing practical field investigation for 1 year and literatures survey simultaneously in 2004. The data from field study were yielded based on voucher specimens. Total number and components of flora of Chirisan National Park is revealed as 1,825 taxa. Among 1,825 taxa, 708 taxa were identified in this field study, and these were comprised 37 orders, 109 families, 382 genera, 590 species 3 subspecies 95 varities 20 forms. Unconfirmed taxa in field but recorded previous literatures were 1,117 taxa, which consist of 41 orders 130 families 500 genera 901 species 9 subspecies 140 varities 67 forms. In conclusion, there may be provisionally 1,825 (708+1,117) taxa in Chirisan National Park. The 43 taxa were firstly found out in this field study. In the floristic data from field study, the number of Korean endemic plants were 31 taxa, the rare and endangered plants which was designated by Korea Forest Service were 21 taxa, the taxa that is more than the third degree among the floristic regional indicator plants which was designated by Korean Ministry of Environment were 40 taxa, and naturalized alien plants to Korea were 27 taxa respectively. Among 1,117 taxa which were not found in this study but recorded in 22 previous literatures, the number of Korean endemic plants were 45 taxa, the rare and endangered plants which was designated by Korea Forest Service were 45 taxa, the taxa that is more than the third degree among the floristic regional indicator plants which was designated by Ministry of Environment were 121 taxa, and naturalized alien plants to Korea were 31 taxa. It can be inferred that the vegetation of Chirisan National Park was changing by some factors and valuable plant resources were tend to diminishing by such as human interference and developments.

• Nuclear Medicine and Molecular Imaging
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• v.42 no.1
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• pp.8-16
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• 2008

Purpose: To assess the effect of extracranial-intracranial (EC-IC) bypass surgery on hemodynamic improvement, we evaluated serial regional cerebral hemodynamic change of the middle cerebral artery (MCA) in symptomatic patients with atherosclerotic occlusion of the internal carotid artery (ICA) or MCA using $^{99m}Tc$-ECD acetazolamide stress brain perfusion SPECT (Acetazolamide SPECT). Materials and Methods: The patients who had suffered a recent stroke with atherosclerotic ICA or MCA occlusion underwent EC-IC bypass surgery and Acetazolamide SPECT at 1 week before and three to six months after surgery. For image analysis, attenuation corrected images were spatially normalized to SPECT templates with SPM2. Anatomical automated labeling was applied to calculate mean counts of each Volume-Of-Interest (VOI). Seven VOIs of bilateral frontal, parietal, temporal regions of the MCA territory and the ipsilateral cerebellum were defined. Using mean counts of 7 VOIs, cerebral perfusion index and perfusion reserve index were calculated. Results: Seventeen patients (M:F =12:5, mean age $53{\pm}2yr$) were finally included in the analysis. The cerebral blood flow of the parietal region increased at 1 week (p = 0.003) and decreased to the preoperative level at 3-6 months (p = 0.003). The cerebrovascular reserve of the frontal and parietal regions increased significantly at 1 week after surgery (p<0.01) and improved further at 3-6 months. Conclusion: Cerebrovascular reserve of the MCA territory was significantly improved at early postoperative period after EC-IC bypass and kept improved state during long-term follow-up, although cerebral blood flow did not significantly improved. Therefore, cerebrovascular reserve may be a good indicator of postoperative hemodynamic improvement resulted from bypass effect.

• Korean Journal of Agricultural and Forest Meteorology
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• v.15 no.2
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• pp.85-101
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• 2013

In order to search for distribution characteristics on C4 plants at the paddy fields, uplands, orchard and roadside in South Korea, vascular plants were investigated. Flora investigation had been carried out from May 2002 to October 2006 at 241 plots. In the results of survey, the flora of these areas consist of 74 families, 231 genera and 352 species totally. The class frequencies were arranged by the order of Magnoliopsidae (255 species), Monocotyledoneae (89 species), Pteropsida (7 species) and Sphenopsida (1 species). The number of species in each investigated agricultural field types was 55 families with 203 species in paddy field, 49 families with 218 species in uplands, 44 families with 115 species in orchard and 48 families with 202 species in roadside respectiviely. Representative species compositions of these four agricultural field types were characterized by Artemisia princeps, Persicaria thunbergii in paddy field, Artemisia princeps, Erigeron annuus in upland and roadside, Commelina communis, Digitaria ciliaris in orchard, respectively. Therefore, indicative species in agricultural ecosystem was Artemisia princeps. Among the investigated 352 species, $C_4$ plants identified were 38 species, and the occurrence ratio of $C_4$ monocotyledonous plants were higher in paddy field, orchard and roadside than upland. Such differences in the occurrence ratio of $C_4$ plants under diverse agricultural field types reflect differences in environmental condition such as micro-climate, soil moisture under various agricultural fields. Dominant $C_4$ monocotyledonous plants were Digitaria ciliaris and Commelina communis, while $C_4$ dicotyledonous plants were Amaranthus mangostanus and Chenopodium album var. centrorubrum in agricultural fields. Naturalized plants were identified as 47 species and occurrence ratio were higher in upland and roadside than other agricultural field types. Among the investigated naturalized plants, $C_4$ plants were Amaranthus mangostanus and Amaranthus retroflexus. Distribution characteristics of representative $C_4$ plants in agricultural field types, revealed that the projected increase in temperature due to climate change may provide better conditions for the growth of $C_4$ plants. Thus, the necessity of long-term monitoring should be conducted on the diffusion of $C_4$ plants that may threaten influence agroecosystem in Korea.

• Tuberculosis and Respiratory Diseases
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• v.57 no.4
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• pp.320-328
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• 2004

Background : Pulmonary vascular changes which occur early in the course of chronic obstructive pulmonary disease (COPD) are prevalent manifestation and later cause pulmonary hypertension, which is a bad prognostic factor in COPD. Beraprost sodium (BPS), an orally active prostacyclin analogue, has been shown to improve survival in patients with primary pulmonary hypertension. This study investigated the effect of BPS in the patients with COPD. Methods : This is a double-blind randomized placebo-controlled, two center clinical trial. Twenty one consecutive patients with COPD were enrolled from June 2003 to June 2004 (patients treated with BPS for 3 months, BPS group, n=11; those with placebo, placebo group, n=10). The baseline demographic, pulmonary function and hemodynamic data were not significantly different between two groups. Results : On echocardiographic examination, trans tricuspid valve pressure gradient has decreased significantly after 3 months with beraprost in the BPS group [17.7(${\pm}11.4$) to 8.2(${\pm}8.9$) mm Hg, p-value<0.05], while there was no significant change in the control group. Six-minute walking distance has decreased in the control group and increased in the BPS group, but there was no statistical significance. Conclusion : In patients with COPD oral administration of BPS reduced the pulmonary arterial pressure. The clinical significance of this finding, that is improving symptoms and natural course of the disease, needs further study.

• Journal of Life Science
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• v.23 no.9
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• pp.1109-1117
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• 2013

Induction of G1 Arrest by Methanol Extract of Lycopus lucidus in Human Lung Adenocarcinoma A549 Cells Lycopus lucidus, a herbaceous perennial, is used as a traditional remedy in East Asia, including China and Korea. It has been reported that L. lucidus has anti-allergic effects, inhibitory effects on cholesterol acyltransferase in high glucose-induced vascular inflammation, and anti-proliferative effects in human breast cancer cells. However, the molecular mechanisms of the anti-cancer effects of L. lucidus have not yet been fully determined. In this study, we evaluated the anti-cancer effect and the mechanism of action of L. lucidus in human lung adenocarcinoma A549 cells using methanol extracts of L. lucidus (MELL). MELL treatment showed cytotoxic activity in a dose-dependent manner and induced G1 arrest in A549 cells. The induction of G1 arrest by MELL was associated with the up-regulation of phospho-CHK2 and the down-regulation of Cdc25A phosphatase. In addition, MELL treatment induced decreased expression of G1/S transition-related proteins, including CDK2, CDK4, CDK6, cyclin D1 and cyclin E. MELL also regulated the mRNA expression of CDK2 and cyclin E. On the other hand, the expression of p53 and the cyclin-dependent kinase inhibitor p21 was not induced by MELL. Collectively, these results suggest that MELL may exert an anti-cancer effect by cell cycle arrest at G1 phase through the ATM/CHK2/Cdc25A/CDK2 pathway in A549 cells.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.77-90
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• 2015

BACKGROUND: The field margins, which are uncultivated area of permanent vegetation located adjacent to field, play an important role in enhancing biodiversity in the agricultural ecosystem. This study focused on floristic characteristics on dry field margins in Korea Peninsula. METHODS AND RESULTS: This study was conducted at margins of 196 dry fields in 32 areas in 8 provinces in May and September 2013. The vascular plants of the dry field margins were listed 347 taxa which contain 73 families, 219 genera, 311 species, 1 subspecies, 30 varieties and 5 forms. Of these 347 taxa, 48 taxa were woody plants and 299 taxa were herbaceous plants. Among total 73 families, Compositae (17.6%) was the most dominant family, and followed Gramineae (15.9%), Polygonaceae (5.8%), Leguminosae (4.9%) and Cyperaceae (3.8%). Based on relative frequency values of survey fields, the appearance frequency of Digitaria ciliaris (89.8%) was the highest, and followed Acalypha australis (86.2%), Artemisia princeps (82.1%), Commelina communis (80.6%) and Portulaca oleracea (80.6%). Disturbance index of dry field margins was 55.0% at national level, and Gangwon region was the highest among the 8 regions at regional level. Life form spectrum was determined to be $Th-R_5-D_4-etype$. CONCLUSION: These results will be used fundamental data for studies to improve or even stabilize the current state of plant biodiversity in agricultural environments.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.31 no.3
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• pp.83-94
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• 2013

The purpose of this study is to offer basic data about prototype conservation of the Orijangnim, natural monument No. 404. For this study, March to August 2010, was a literature surveys and field research, and based on this, the Delphi survey of local residents and experts meeting were diagnosed with forest back ground, spatial extent, in the form of forests, flora and vegetation of the surrounding area, so examined closely the original form. Range of the Orijangnim was presumed from Jacheon middle school entrance($N36^{\circ}05^{\prime}59.9^{{\prime}{\prime}}/E128^{\circ}54^{\prime}32.9^{{\prime}{\prime}}$) to Odong of Hwabukmyeon village entrance($N36^{\circ}05^{\prime}31.0^{{\prime}{\prime}}/E128^{\circ}54^{\prime}32.5^{{\prime}{\prime}}$). The Orijangnim in the original form of the existing forest surrounding the village along gohyeon river going back a long S-shaped about 2km is considered. The investigated vascular plants consist of total 198 taxa : 2 forms, 20 varieties, 179 species, 152 Genera, 72 Families. Most of the 60 kinds of large land plants lots of whole plants accounted for 33.3%. A main vegetation of the Orijangnim have been distributed 473 objects of 21 species, and evergreen conifers is 19% to 92 objects, on the other hand, deciduous broadleaf tree is 79% to 373 objects, so the Orijangnim can be maxed forest that deciduous broadleaf tree is dominant. When consider the composition purpose of forest, environment of location, tree size, surrounding flora and vegetation, because an original vegetation of Orijangnim is suggested Zelkova serrata, Salix glandulosa, Celtis sinensis Pers., Quercus variabilis, Quercus acutissima , Sophora japonica L., Cornus walteri Wanger. we must restore around such species of tree. Cedrus deodara (Roxb.) Loudon, Chaenomeles sinensis, Prunus yedoensis Matsum., Ginkgo biloba , Pinus thunbergii Parl., Populus tomentiglandulosa T. Lee is considered to have been planted in the last.

• IMMUNE NETWORK
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• v.1 no.1
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• pp.87-94
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• 2001

Background: Cytokine-mediated ex vivo expansion has been proposed as a means of increasing the number of cord blood (CB) hematopoietic stem cells for transplantation. As well as stem cell number, stromal cells are necessary for functional maturation of hematopoiesis. The purpose of this study was to analyze the development of stromal cells during ex vivo expansion of CB $CD34^+$ cells. Methods : $CD34^+$ cells were purified from CB by magnetic bead selection. The levels of of interleukin-3, interleukin-$1{\beta}$, interleukin-6, granulocyte macrophagecolony stimulating factor and tumor necrosis factor-${\alpha}$ were measured in culture supernatants on 0, 1, 2, and 3 weeks, using ELISA techniques. CB $CD34^+$ cells were expanded in Iscoves modified Dulbeccos medium in the presence of several cytokines. The expression of E-selectin, vascular cell adhesion molecule-1, intercellular adhesion molecule-1, platelet/endothelial cell adhesion molecule-1, von Willebrand factor, vimentin, and CD14 in newly developed stromal cells was examined by immunocytochemical method. Relevant extracellular matrix (ECM) proteins and proper cytokines were also assayed for the most suitable condition for expansion of stromal cells. Results: Several cytokines were found to have been produced by CB $CD34^+$ cells as well as bone marrow-derived $CD34^+$ cells. During ex vivo expansion of CB $CD34^+$ cells, stromal cells appeared in the culture by day 4 and expanded over the following 7-10 days before being confluent by day 2 1. These cells expressed surface markers characteristic of cells of endothelial lineage. Furthermore, these stroaml cells also expanded effectively when treated with thrombopoietin+flt-3 ligand+stem cell factor+leukemia inhibitory factor or 0.1% poly-L-lysine-coated wells. Conclusion: Stromal cells were developed during ex vivo expansion of CB $CD34^+$ cells and that this development could be enhanced further by treating the stromal cells with cytokines or ECM.

• The korean journal of orthodontics
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• v.26 no.4
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• pp.359-371
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• 1996

The purpose of this study was to investigate the changes about the cellular activity on DNA synthesis in the periodontal ligament of dog, in which experimental tooth movement was performed. A control and 5 experimental dogs, one and half year in age, were studied. Light force (50-75g) was applied by placing open-coil spring between left mandibular premolars ; heavy force (250-300g), between right mandibular premolars. Experimental dogs were sacrificed after infusion of bromodeoxyuridine(BrdU), at 12 hours, 1, 3, 7 and 14 days after force application, respectively. And the histologic and the immunohistochemical evaluation were performed on the obtained periodontal tissue around mandibular premolars, using anti-romodeoxyuridine antibody, which can indicate proliferating cells. The results were as follows: 1. The tearing of periodontal ligament and the vascular dilatation at tension side were observed in 12 hours, increasing until 3 days. After then it decreased; Such a finding was more evident in heavy force group than in light force group. 2. The hyalinization of the periodontal ligament and the activity of osteoclast at pressure side were observed in 12 hours, increasing until 3 days. But from 7 days on, it decreased; Such a finding was more evident in heavy force group than in light force group. 3. The BrdU expression in the control group was positive, mainly in the oral epithelium and the fibroblasts in the periodontal ligament, but negative in bone cells in periodontal ligament. 4. The BrdU expression in the experimental group was more positive in tension side than in pressure side; The expression was a little more positive in the periapical area than in the cervical area of tooth. 5. The BrdU expression in light force group was the highest in 1 day, after which it decreased; In heavy force group, it was the highest in 12 hours, after which it decreased. But in 14 days, there was no difference between the experimental group and control group.