• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.34-42
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• 2008

Water quality standards (WQS) are mandatory to guarantee the human health and protection of aquatic ecosystems, and maintain the condition of suitable water quality. The present WQS for the protection of human health in Korea contain nine substances (As, Cd, $Cr^{6+}$, CN, Pb, Hg, ABS, organophosphorus compounds and PCBs), but it is insufficient to preserve the human and aquatic ecosystem from a variety of chemicals. Therefore, it is necessary to expand the substance of WQS for the protection of human health. In this study, we chose the 20 chemicals from 43 chemicals of the project entitled 'Development of Integrated Methodology for Evaluation of Water Environment'. The methodology for calculating water quality criteria was amended from the US Environmental Protection Agency (US EPA)'s equation for deriving ambient water quality criteria for the protection of human health. The factors including fish intake, drinking water intake, and human body weight used in the equation reflected Korean situations. The monitoring values were derived from the water quality monitoring data in Korean four main rivers. The orders of priorities of chemicals were evaluated by human health risk assessment, and the proposed WQS was derived by technical and economic analyses. These results were reflected to expand the WQS for the protection of human health.

• Animal Bioscience
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• v.36 no.1
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• pp.144-155
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• 2023

Objective: Adipocyte differentiation is regulated by a variety of functional genes and noncoding RNAs. However, the role of miRNAs in lipid deposition of goat white adipose tissue is still unclear. Therefore, this study revealed the miRNA expression profile in goat subcutaneous adipocytes by sRNA-seq. Methods: The miRNA expressed in goat subcutaneous preadipocytes and the mature adipocytes were sequenced by sRNA-seq. The differentially expressed miRNAs (DEm) were screened and gene ontology (GO) and Kyoto encyclopedia for genes and genomes (KEGG) analyses were performed. Gain-of-function and loss-of-function combined with oil red O staining, Bodipy staining, and quantitative reverse-transcription polymerase chain reaction (qPCR) were utilized to determine the effect of miR-133a-3p on adipocyte differentiation. Results: A total of 218 DEm were screened out. The target genes of these DEm were significantly enriched in GO items such as biological regulation and in KEGG terms such as FAK signaling pathway and MAPK signaling pathway. qPCR verified that the expression trend of miRNA was consistent with miRNA-seq. The gain-of-function or loss-of-function of miR-133a-3p showed that it promoted or inhibited the accumulation of lipid droplets, and CCAAT enhancer binding protein α (C/EBPα) and C/EBPβ were extremely significantly up-regulated or down-regulated respectively (p<0.01), the loss-of-function also led to a significant down-regulation of peroxisome proliferator activated receptor gamma (PPARγ) (p<0.01). Conclusion: This study successfully identified miRNAs expression patterns in goat subcutaneous adipocytes, and functional identification indicates that miR-133a-3p is a positive regulator of the differentiation process of goat subcutaneous adipocytes. Our results lay the foundation for the molecular mechanism of lipid deposition in meat-source goats from the perspective of miRNA.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.3
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• pp.156-160
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• 2020

This study was conducted at the National Institute of Animal Science from 2010 to 2017. As a variety that is sufficiently productive in the southern regions to replace imported varieties and sufficiently cold-resistant to be cultivated in the central-northern regions, "IR605" was developed and submitted to the Korea Seed & Variety Service in an application for protection. The novel Italian ryegrass variety "IR605" is a diploid with green leaves, a semi-erect growth habit before wintering, and an erect growth habit in the spring. "IR605" was a medium maturing variety with a heading date of around May 15th. "IR605" had a flag leaf width of 9.9 mm, flag leaf length of 26.7 cm, and plant length on the heading date of 100 cm, which was approximately 5 cm longer than "Kowinearly." The stem thickness and ear length of "IR605" are 0.08 mm thicker and 0.5 cm longer than those of "Kowinearly", respectively. The cold-resistance of "IR605" was weaker than that of "Kowinearly", but strong enough to be cultivated in Pyeongchang, Gangwon province. The dry matter yield of "IR605" (9,308 kg/hectare) was 20% higher than that of "Kowinearly", which was further pronounced in the southern region of Haenam, where there was a 52% increased (p < 0.05). The in vitro dry matter digestibility of "IR605" was 68.4% at which was slightly higher than that of "Kowinearly", The total digestible nutrients was 58.5%, which was slightly lower than "Kowinearly". Overall, the feed quality characteristics of "IR605" were similar to those of "Kowinearly".

• Journal of Forest and Environmental Science
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• v.24 no.1
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• pp.15-25
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• 2008

Social and scientific changes, i.e., global warming, desertification, pollution, biodiversity, bioenergy, plant variety protection, biotechnology, timber demand, reforestation in North Korea, and etc., were reviewed for new strategy of forest tree breeding in Korea. Diversified breeding goals, globalization of breeding target species, multidisciplinary research approaches, manpower networking, establishment of new administrative and research units in KFS and KFRI were proposed. Principles suggested for new tree breeding strategy are: 1) multi-disciplinary approach in settlement of objectives, breeding methods, and etc., 2) expansion of target trees including foreign species, 3) fulfillment of both domestic and international demands for forest tree breeding, 4) establishment of breeding program well-grounded on genetic resources conservation, 5) acknowledgement of breeding products (i.e., variety, technique, gene, and etc.) as goods, and 6) provision of more research opportunities for young scientists. Lastly, ongoing tree breeding project in Indonesia and NTFP R&D Center at the College of Forest and Environmental Sciences, Kangwon National University were introduced as examples of desirable breeding projects based on target species diversification, multidisciplinary approach, and manpower networking.

• BMB Reports
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• v.40 no.2
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• pp.226-231
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• 2007

Housekeeping genes are widely used as internal controls in a variety of study types, including real time RT-PCR, microarrays, Northern analysis and RNase protection assays. However, even commonly used housekeeping genes may vary in stability depending on the cell type or disease being studied. Thus, it is necessary to identify additional housekeeping-type genes that show sample-independent stability. Here, we used statistical analysis to examine a large human microarray database, seeking genes that were stably expressed in various tissues, disease states and cell lines. We further selected genes that were expressed at different levels, because reference and target genes should be present in similar copy numbers to achieve reliable quantitative results. Real time RT-PCR amplification of three newly identified reference genes, CGI-119, CTBP1 and GOLGAl, alongside three well-known housekeeping genes, B2M, GAPD, and TUBB, confirmed that the newly identified genes were more stably expressed in individual samples with similar ranges. These results collectively suggest that statistical analysis of microarray data can be used to identify new candidate housekeeping genes showing consistent expression across tissues and diseases. Our analysis identified three novel candidate housekeeping genes (CGI-119, GOLGA1, and CTBP1) that could prove useful for normalization across a variety of RNA-based techniques.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.11a
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• pp.195-195
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• 2003

Combustion generated fine particles, defined as those with aerodynamic diameters less than 2.5 m, have come under increased regulatory scrutiny because of suspected links to adverse human health effects. Transition metals are of particular interest due to the results of a number of studies that have shown cardiopulmonary damage associated with exposure to these elements and their presence in coal, residual fuel oils, sewage sludge, and other combusted fuels and wastes. This lecture will review results from multi-di sciplinary studies being conducted at EPA and elsewhere examining the physical, chemical, and toxicological characteristics of combustion generated particles. The research describes how collaborative work between combustion engineers and health scientists can provide insight on how combustion processes affect particle properties and subsequent health effects as measured by a combination of in-vitro and in-vivo studies using a variety of animal models. The focus of this lecture is on the interdisciplinary approach required to address the problem. Difficulties are discussed. Engineering aspects involved in this approach are described in detail. Physical and chemical characterizations are performed using a variety of analytical approaches including new techniques of x-ray absorption fine structure (XAFS) spectroscopy and x-ray absorption near-edge structure (XANES) deconvolution of these spectra to gather metal speciation information.

• Journal of Arbitration Studies
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• v.23 no.2
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• pp.165-190
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• 2013

As intellectual property rights are perceived as the key element of creating added values and securing competitiveness, the result of intellectual property rights disputes play an important role in the success of companies. As seen from above strong points of an Alternative Dispute Resolution (ADR) system in Chapter III, intellectual property rights disputes increasingly tend to be resolved by ADR rather than litigation. Discussions about and operation of ADR are already being actively carried out in many countries, and major ADR institutions have been acquiring experience in a variety of intellectual property rights disputes. To enhance the use and recognition of ADR as the way of resolving the Intellectual Property Rights disputes in Korea, this study suggested the following three ways. First, domestic ADR institutions, the Korean Commercial Arbitration Board (KCAB) will need to establish cooperative systems with prominent overseas institutions to lead the disputing parties to fair resolutions as well as to instill trust in international arbitration institutions. Second, they will need to contribute to the promotion of arbitration systems throughout society by developing and applying a variety of arbitration systems as well as securing a pool of professionals. Finally, the arbitration rules will need to be continuously improved to deal with disputes promptly and reinforce privacy protection.

• FLOWER RESEARCH JOURNAL
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• v.19 no.3
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• pp.181-186
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• 2011

A new spray chrysanthemum cultivar 'Yellow Candy' was released by National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA), in 2008. The cross was made in 2003 between 'Restone' and 'Lollipop'. Trials were conducted from 2006 to 2008 for the evaluation and selection of this cultivar, including shading cultures in summer and retarding cultures in spring. The natural flowering time of 'Yellow Candy' is late October, but year-round flowering is possible by photoperiodic control. It has pompon flower type with yellow petals and yellowish red flower center. The growth of plant is very vigorous. The diameter of flower is 4.3 cm. Number of flowers per stem and petals per flower are 8 and 184, respectively. Days to flowering under the short day treatment is about 58.5 and its vase life is 18.5 days in autumn season. 'Yellow Candy' was applied as No. 2009-177 on Feb. 18, 2009 for variety protection and the plant variety protection rights have been registered as No. 3247 on August 3, 2010 at the Korea Seed and Variety Service.

• Journal of Digital Contents Society
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• v.19 no.4
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• pp.711-717
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• 2018

The breakthrough in computer and network has facilitated a variety of information exchange. However, at the same time, malicious users and groups are attacking vulnerable systems. Intrusion Detection System(IDS) detects malicious behaviors through network packet analysis. However, it has a burden of processing a large amount of packets in a short time. Therefore, in order to solve these problem, we propose a network intrusion detection system that operates at kernel level to improve detection performance at user level. In fact, we confirmed that the network intrusion detection system implemented at kernel level improves packet analysis and detection performance.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.11 no.2
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• pp.189-196
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• 2011

As internet is getting advanced day by day, digital contents have been developed in various areas as killer content in the IT industry. It needs to develop lots of complicated digital content protect systems due to the enhancement and variety of user's multimedia devices. Although there are lots of protect systems based on DRM(Digital Rights Management) technology, it is difficult to provide secure protection because of the problems resulting from analog hole problem, inefficiency of key sharing and various independent protect technologies. Thus, in this paper, we propose a novel authentication and protect system based on RFID(Radio Frequency Identification) technology to solve the problems and show possibility of free content duplication and efficient contents management in smart home environments.