• Title/Summary/Keyword: Variety protection

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Application of RAPD markers for characterization of ${\gamma}$-ray-induced rose mutants and assessment of genetic diversity

  • Chakrabarty, D.;Datta, S.K.
    • Plant Biotechnology Reports
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    • v.4 no.3
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    • pp.237-242
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    • 2010
  • Six parent and their 12 gamma ray-induced somatic flower colour mutants of garden rose were characterized to discriminate the mutants from their respective parents and understanding the genetic diversity using Random amplification of polymorphic DNA (RAPD) markers. Out of 20 primers screened, 14 primers yielded completely identical fragments patterns. The other 7 primers gave highly polymorphic banding patterns among the radiomutants. All the cultivars were identified by using only 7 primers. Moreover, individual mutants were also distinguished by unique RAPD marker bands. Based on the presence or absence of the 48 polymorphic bands, the genetic variations within and among the 18 cultivars were measured. Genetic distance between all 18 cultivars varied from 0.40 to 0.91, as revealed by Jaccard's coefficient matrix. A dendrogram was constructed based on the similarity matrix using the Neighbor Joining Tree method showed three main clusters. The present RAPD analysis can be used not only for estimating genetic diversity present in gamma ray-induced mutants but also for correct identification of mutant/new varieties for their legal protection under plant variety rights.

THE EFFECT OF COOLSTORE DESIGN AND OPERATION ON AIR RELATIVE HUMIDITY

  • Amos, N.D.;Cleland, D.J.;Cleland, A.C.;Banks, N.H.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1993.10a
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    • pp.433-442
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    • 1993
  • Coolstore air relative humidity (RH) is an important factor affecting the quality of horticultural products, particularly via product moisture loss. RH also has an important effect on the performance of the refrigeration evaporators and can affect the strength of paper-based packaging materials. In a large New Zealand apple coolstore, RH increased from about 75% early in season to 90% at the end, as activities in the coolstore and external conditions changed. A steady-state analysis of sensible and latent heat entry and heat removal during four typical operational periods over the season was carried out. Predicted RH was in good agreement with measured dat. For the coolstore studied, evaporator surface area and the occurrence of pre-cooling within the coolstore were the design and operational factors having greatest effect on RH. Door protection and management, and floor insulation were the next more significant factors. The method of analysis has more general application and ould be used in a variety of situations so that design for optimum RH can be performed systematically.

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Transport of chloride through saturated soil column: An experimental study

  • Patil, S.B.;Chore, H.S.
    • Advances in environmental research
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    • v.4 no.2
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    • pp.105-117
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    • 2015
  • The groundwater is a very important part of the environment and must be protected for the benefit of the present and future generation. The contamination of soil and groundwater by chemicals has become an increasing concern in the recent past. These chemicals enter the groundwater system by a wide variety of mechanisms, including accidental spills, land disposal of domestic and industrial wastes and application of agricultural fertilizers. Once introduced into an aquifer, these contaminants will be transported by flowing groundwater and may degrade water quality at nearby wells and streams. For improving the management and protection of groundwater resources, it is important to first understand the various processes that control the transport of contaminants in groundwater. Predictions of the fate of groundwater contaminants can be made to assess the effect of these chemicals on local water resources and to evaluate the effectiveness of remedial actions. In this study, an attempt has been made to investigate the behaviour of solute transport through porous media using laboratory experiments. Sodium chloride was used as a conservative chemical in the experiment. During the experiment, pulse boundary condition and continuous boundary conditions were used. Experimental results have been presented for conservative solute transport in the sand. The pattern of the break through curve remains almost same in all the cases of varying flow rate and initial concentration of conservative chemical.

Chemopreventive Efficacy of Moringa oleifera Pods Against 7, 12-Dimethylbenz[a]anthracene Induced Hepatic Carcinogenesis in Mice

  • Sharma, Veena;Paliwal, Ritu;Janmeda, Pracheta;Sharma, Shatruhan
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.6
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    • pp.2563-2569
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    • 2012
  • Oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage in a variety of liver disorders. Hence there is a great demand for the development of agents with potent antioxidant effect. The aim of the present investigation is to evaluate the efficacy of Moringa oleifera as a hepatoprotective and an antioxidant against 7, 12-dimethylbenz[a]anthracene induced hepatocellular damage. Single oral administration of DMBA (15 mg/kg) to mice resulted in significantly (p<0.001) depleted levels of xenobiotic enzymes like, cytochrome P450 and b5. DMBA induced oxidative stress was confirmed by decreased levels of reduced glutathione (GSH) and glutathione-S-transferase (GST) in the liver tissue. The status of hepatic aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) which is indicative of hepatocellular damage were also found to be decreased in DMBA administered mice. Pretreatment with the Moringa oleifera (200 and 400 mg/kg) orally for 14 days significantly reversed the DMBA induced alterations in the liver tissue and offered almost complete protection. The results from the present study indicate that Moringa oleifera exhibits good hepatoprotective and antioxidant potential against DMBA induced hepatocellular damage in mice that might be due to decreased free radical generation.

Surgical treatment of the disease involving ascending aorta (상행 대동맥 질환의 외과적 치료)

  • 백완기
    • Journal of Chest Surgery
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    • v.27 no.7
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    • pp.581-586
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    • 1994
  • From February 1985 to February 1993, 18 operations were performed in 17 patients for treatment of aneurysmal disease [n=12] and/or dissection of the ascending aorta [n=6]. The ages ranged from 26 to 69 years [mean 44.3 $\pm$ 11.0 years].The proposed operations include composite graft replacement of aortic valve and ascending aorta with coronary reimplantation in 11, graft replacement of ascending aorta alone in 5, aortic valve replacement and supracoronary graft replacement in 1 and ascending aorta to abdominal aorta bypass with thromboexclusion of descending aorta in one patient. Both Bentall [n=6] and Cabrol [n=5] technique were utilized for reimplantation of coronary arteries.Concomitant replacement of aortic arch and arch vessel reconstruction was necessary in two patients. Hypothermic circulatory arrest was utilized in 6 patients. Recently, four patients were managed on warm blood continuous cardioplegia via retrograde route. There were no operative deaths. No significant postoperative complications were noted. Postoperative follow up was complete in 15 patients from 1 month to 72 months. Redo operation was necessary in one patient who had suffered from distal recurrence of dissection 5 years after successful Bentall operation. The other patients are all in excellent clinical condition. From our early experience with those 17 cases, we assume that satisfactory operative result could be achieved with a variety of surgical technique including hypothermic circulatory arrest. In addition, continuous perfusion of warm blood cardioplegia via retrograde route is supposed to be beneficial in selected cases.

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Traditional Agricultural Landscape as ail Important Model of Ecological Restoration in Japan

  • Toshihiko, Nakamura
    • The Korean Journal of Ecology
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    • v.25 no.1
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    • pp.19-24
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    • 2002
  • The traditional Japanese agricultural landscape, In which a set of varied land-use patches functions as a sustainable ecosystem landscape unit, not only provides the local people tilth a stable food supply, but also offers a variety of habitats to many species of wildlife. Therefore, remaining natural habitats including those in the traditional agricultural landscape should be maintained whenever possible. In addition, restoration work should be implemented in areas where the natural habitat has been destroyed or severely degraded by human activities. This basic approach to the natural environment is a combination of maintenance and restoration. Types of maintenance and restoration can be classified into three categories according to the countermeasures employed: preservation, conservation and protection types of maintenance, and improvement, reconstruction and creation types of restoration. Four steps are proposed for ecological restoration and maintenance of a target area: exploration, diagnosis, prescription and care. In this process, a model for approaching the goal is important. One of the most important models should center on the traditional agricultural landscape involving a sustainable farming ecosystem. It is necessary to protect traditional landscapes and ecosystems from the degrading impact of urbanization and industrialization, as well as to enhance efforts at restoration.

Present status and prospects of Haemophilus influenzae type b(Hib) immunization (Haemophilus influenzae type b(Hib) 백신 접종의 현황과 전망)

  • Kim, Kyung Hyo
    • Clinical and Experimental Pediatrics
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    • v.49 no.3
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    • pp.242-250
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    • 2006
  • Haemophilus influenzae type b(Hib) conjugate vaccines prevent Hib disease in individuals and reduce the carriage and transmission of the organism in the community. The incidence of Hib disease has been decreased dramatically in a diverse range of countries through the use of a variety of conjugate vaccines and vaccine schedules. In some countries, the vaccine has caused a near-disappearance of invasive Hib disease through a combination of direct protection and herd immunity. The effectiveness of the vaccine was not modified by the type of conjugate vaccine, the number of doses given(two, three or four), age at first vaccination(two months, 42 to 90 days, three months) and whether the vaccine was tested in an industrialized or developing country. Over 15 years of international experience with vaccines has also demonstrated that they are safe. In 2004, Hib vaccines were adapted in routine immunization in 92 countries in the world. Decisions regarding the use of the Hib vaccine in routine immunization schedules depend not only on the effectiveness and efficacy of the vaccine but also on factors such as burden of disease, vaccine cost, and competing priorities. In Korea, Hib disease burden seemed to be lower than other developed countries(~10/100,000). Moreover Hib vaccines showed excellent immunogenicity in Korean children in many studies. Therefore, a potential approach to economize the cost of Hib vaccines could be to explore the possibilities of using reduced vaccine doses for immunization as some other countries.

Message in a Bottle: Chemical Biology of Induced Disease Resistance in Plants

  • Schreiber, Karl;Desveaux, Darrell
    • The Plant Pathology Journal
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    • v.24 no.3
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    • pp.245-268
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    • 2008
  • The outcome of plant-pathogen interactions is influenced significantly by endogenous small molecules that coordinate plant defence responses. There is currently tremendous scientific and commercial interest in identifying chemicals whose exogenous application activates plant defences and affords protection from pathogen infection. In this review, we provide a survey of compounds known to induce disease resistance in plants, with particular emphasis on how each compound was originally identified, its putative or demonstrated mechanism of defence induction, and the known biological target(s) of each chemical. Larger polymeric structures and peptides/proteins are also discussed in this context. The quest for novel defence-inducing molecules would be aided by the capability for high-throughput analysis of candidate compounds, and we describe some issues associated with the development of these types of screens. Subsequent characterization of hits can be a formidable challenge, especially in terms of identifying chemical targets in plant cells. A variety of powerful molecular tools are available for this characterization, not only to provide insight into methods of plant defence activation, but also to probe fundamental biological processes. Furthermore, these investigations can reveal molecules with significant commercial potential as crop protectants, although a number of factors must be considered for this potential to be realized. By highlighting recent progress in the application of chemical biology techniques for the modulation of plant-pathogen interactions, we provide some perspective on the exciting opportunities for future progress in this field of research.

Framework for Deriving Water Quality Criteria of Toxic Substances (수질유해물질에 대한 수질환경기준 설정체계)

  • Chung, Yun-Chul;Ko, Dae-Hyun
    • Journal of Korean Society on Water Environment
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    • v.21 no.4
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    • pp.305-313
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    • 2005
  • In these days, water environment is getting threatened by a variety of toxic pollutants discharged from industries. However, environmental standards and regulations in Korea may be in straitened circumstances to protect the water environment from it. Therefore, the purpose of this review is to compare the management state of the toxic substances in water environment and to present the framework for deriving water quality criteria in USA and Japan. To conserve the water environment from the toxic pollutants more efficiently, the following considerations could be suggested in standards and regulation in Korea. Firstly, there should be consistency of regulated pollutants in drinking water quality standard, water quality standards and permissible wastewater discharge standards. Secondly, in case of deriving the water quality standards, it is required to consider the conservation of the aquatic ecosystem as well as the protection of human health. Finally, it is indispensable to make risk-based approach in management of toxic pollutants in water environment.

Investigations into Coarsening Continuous Variables

  • Jeong, Dong-Myeong;Kim, Jay-J.
    • The Korean Journal of Applied Statistics
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    • v.23 no.2
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    • pp.325-333
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    • 2010
  • Protection against disclosure of survey respondents' identifiable and/or sensitive information is a prerequisite for statistical agencies that release microdata files from their sample surveys. Coarsening is one of popular methods for protecting the confidentiality of the data. Grouped data can be released in the form of microdata or tabular data. Instead of releasing the data in a tabular form only, having microdata available to the public with interval codes with their representative values greatly enhances the utility of the data. It allows the researchers to compute covariance between the variables and build statistical models or to run a variety of statistical tests on the data. It may be conjectured that the variance of the interval data is lower that of the ungrouped data in the sense that the coarsened data do not have the within interval variance. This conjecture will be investigated using the uniform and triangular distributions. Traditionally, midpoint is used to represent all the values in an interval. This approach implicitly assumes that the data is uniformly distributed within each interval. However, this assumption may not hold, especially in the last interval of the economic data. In this paper, we will use three distributional assumptions - uniform, Pareto and lognormal distribution - in the last interval and use either midpoint or median for other intervals for wage and food costs of the Statistics Korea's 2006 Household Income and Expenditure Survey(HIES) data and compare these approaches in terms of the first two moments.