Browse > Article
http://dx.doi.org/10.7314/APJCP.2012.13.6.2563

Chemopreventive Efficacy of Moringa oleifera Pods Against 7, 12-Dimethylbenz[a]anthracene Induced Hepatic Carcinogenesis in Mice  

Sharma, Veena (Department of Bioscience and Biotechnology, Banasthali University)
Paliwal, Ritu (Department of Bioscience and Biotechnology, Banasthali University)
Janmeda, Pracheta (Department of Bioscience and Biotechnology, Banasthali University)
Sharma, Shatruhan (MAI)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.13, no.6, 2012 , pp. 2563-2569 More about this Journal
Abstract
Oxidative stress is a common mechanism contributing to initiation and progression of hepatic damage in a variety of liver disorders. Hence there is a great demand for the development of agents with potent antioxidant effect. The aim of the present investigation is to evaluate the efficacy of Moringa oleifera as a hepatoprotective and an antioxidant against 7, 12-dimethylbenz[a]anthracene induced hepatocellular damage. Single oral administration of DMBA (15 mg/kg) to mice resulted in significantly (p<0.001) depleted levels of xenobiotic enzymes like, cytochrome P450 and b5. DMBA induced oxidative stress was confirmed by decreased levels of reduced glutathione (GSH) and glutathione-S-transferase (GST) in the liver tissue. The status of hepatic aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) which is indicative of hepatocellular damage were also found to be decreased in DMBA administered mice. Pretreatment with the Moringa oleifera (200 and 400 mg/kg) orally for 14 days significantly reversed the DMBA induced alterations in the liver tissue and offered almost complete protection. The results from the present study indicate that Moringa oleifera exhibits good hepatoprotective and antioxidant potential against DMBA induced hepatocellular damage in mice that might be due to decreased free radical generation.
Keywords
M. oleifera; 7; 12-dimethylbenz[a]anthracene; xenobiotic; hepato-protective; oxidative stress;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Arabshahi DS, Devi V, Urooj A (2007). Evaluation of antioxidant activity of some plant extracts and their heat, pH and storage stability. Food Chem, 100, 1100-5.   DOI
2 Bennett RN, Mellon FA, Foidl N, et al (2003). Profiling glucosinolates and phenolics in vegetative and reproductive tissues of the multi-purpose trees Moringa oleifera L. (Horseradish tree) and Moringa stenopetala L. J Agri Food Chem, 51, 3546-53.   DOI
3 Bharali R, Tabassum J, Azad MRH (2003). Chemomodulatory effect of Moringa oleifera, Lam. on hepatic carcinogen metabolizing enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac J Cancer Prev, 4, 131-9.
4 Burton GW, Foster DO, Prly B (1985). Biological antioxidants. Phil Trans R Soc Lond, 311, 565-78.   DOI
5 Chattopadhyay RR (2003). Possible mechanism of hepatoprotective activity of Azadirachta indica leaf extract: part II. J of Ethnopharmacol, 89, 217-9.   DOI   ScienceOn
6 Das BN, Biswas BK, Moghal MMR, et al (2010). Study of Analgesic Activity of Bark of Xeromphis spinosa. Int J of Comprehensive Pharm, 1, 1-3.
7 De Leve LD, Wang X, Kuhlenkamp JF, et al (1996). Toxicity of azathioprine and monocrotaline in murine sinusoidal endothelial cells and hepatocytes: the role of glutathione and relevance to hepatic venoocclusive disease. Hepatol, 23, 589-99.   DOI
8 Vadlapudi V, Naidu KC (2010). In vitro bioautography of different Indian medicinal plants. Drug Invent Today, 2, 53-6.
9 Zak B (1977). Cholesterol methodologies: a review. Clin Chem, 23, 1201-14.
10 Fakurazi S, Hairuszah I, Nanthini U (2008). Moringa oleifera Lam prevents acetaminophen induced liver injury through restoration of glutathione level. Food & Chemical Toxicol, 46, 2611-5.   DOI
11 Gibson GG, Skett P (eds.) (1994). Introduction to drug metabolism. Blackie Academic and Professonal, London, 217-58.
12 Guevara AP, Vargas C, Sakurai H, et al (1999). An antitumor promoter from Moringa oleifera Lam. Mut Res, 440, 181-8.   DOI
13 Habig WH, Pabst MJ, Jakoby WB (1974). Glutathione S transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 7130-9.
14 IARC. 1973. Some Polycyclic Aromatic Hydrocarbons and Heterocyclic Compounds. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, vol. 3. Lyon, France: International Agency for Research on Cancer. pp 271.
15 IARC (1983). Polynuclear Aromatic Compounds, Part 1. Chemical, Environmental and Experimental Data. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, vol. 32. Lyon, France: International Agency for Research on Cancer.
16 IARC (1984). Polynuclear Aromatic Compounds, Part 3. Industrial Exposures in Aluminum Production, Coal Gasification, Coke Production, and Iron and Steel Founding. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, vol. 34. Lyon, France: International Agency for Research on Cancer. 219 pp.
17 IARC (1985). Polynuclear Aromatic 4. Bitumens, Coal Tars and Derived Products, Shale Oils and Soots. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, vol. 35. Lyon, France: International Agency for Research on Cancer. 271 pp.
18 IARC (1987). Overall Evaluations of Carcinogenicity. IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Humans, Supplement 7. Lyon, France: International Agency for Research on Cancer. 440 pp.
19 Janmeda P, Sharma V, Singh L, et al (2011). Chemopreventive effect of hydroethanolic extract of Euphorbia neriifolia leaves against DENA-induced renal carcinogenesis in mice. APJCP, 12, 677-83.
20 James LP, Mayeux PR, Hinson JA (2003). Acetaminopheninduced hepatotoxicity. Drug Metab Dispos, 31, 1499-506.   DOI   ScienceOn
21 Jollow DJ, Mitchell JR, Zampagilone N, et al (1974). Bromobenzene induced liver necrosis. Protective role of glutathione and evidence for 3, 4-bromobenzene oxide as the hepatotoxic intermediate. Pharmacol, 11, 151-69.   DOI   ScienceOn
22 Kettrer B (1998). Glutathione-S-trensferase and prevention of cellular free radical damage. Free Rad Res, 28, 647-58.   DOI   ScienceOn
23 Kumar A, Pari L (2003). Antioxidant action of Moringa oleifera Lam (drumstick) against antitubercular drugs induced lipid peroxidation in rats. J Med Food, 6, 255-9.   DOI   ScienceOn
24 Lalas S, Tsaknis J (2002). Extraction and identification of natural antioxidants from the seeds of Moringa oleifera tree variety of Malavi. J Am Oil Chem Soc, 79, 677-83.   DOI
25 Lowry OH, Rosebrough NJ, Farr AL, et al (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193, 265-75.
26 Mohandas J, Marshall JJ, Duggin GG, et al (1984). Differerential distribution of glutathione and glutathione related enzymes in rabbit kidney. Biochem Pharmacol, 33, 1801-7.   DOI   ScienceOn
27 Okuda K (1997). Hepatocellular carcinoma: clinicopathological aspects. J Gastroenterol Hepatol, 2, 314-8.
28 Omura T, Sato R (1964). The carbon monoxide binding pigment of liver. J Biol Chem, 239, 2370-8.
29 Paliwal R, Sharma V, Pracheta, et al (2011b). Antinephrotoxic effect of administration of moringa oleifera lam in amelioration of DMBA-induced renal carcinogenesis in Swiss albino mice. Biol med, 3, 27-35.
30 Paliwal R, Sharma V, Pracheta (2011a). A Review on horse radish tree (moringa oleifera): a multipurpose tree with high economic and commercial importance. Asian J Biotechnol, 3, 317-28.   DOI
31 Paliwal R, Sharma V, Pracheta, et al (2011c). Hepatoprotective and antioxidant potential of moringa oleifera pods against DMBA-induced hepatocarcinogenesis in male mice. Int J Drug Dev Res, 3, 128-38.
32 Paliwal R, Sharma V, Pracheta, et al (2011d). Elucidation of free radical scavenging and antioxidant activity of aqueous and hydro-ethanolic extracts of moringa oleifera pods. Res J Pharm Technol, 4, 566-71.
33 Panteghini M (1990). Aspartate amino transferase isoenzymes. Clin. Biochem, 23, 311-9.   DOI
34 Parimalakrishnan S, Dey A, Manavalan R (2009). Effect of methanolic extract of Cleome chelidonii on Drug metabolising enzymes, antioxidant status and chemomodulatory efficacy of mice. J Basic App Sci, 5, 37-46.
35 Parmar J, Sharma P, Verma P, et al (2011). Modulation of DMBA induced biochemical and histopathological changes by Syzygium cumini seed extract during skin carcinogenesis. Int J Cur Biomed Phar Res, 1, 24-30.
36 Radhakrishnan V, Park CM, Ravikumar V, et al (2007) Inhibitory effects of histone deacetylase inhibitor depsipeptide on benzo(a)pyrene- and cyclophosphamide-induced genotoxicity in Swiss albino mice. Int J Toxicol, 26, 47-50.   DOI
37 Ramakrishnan G, Augustine TA, Jagan S, et al (2007). Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats. Exp Oncol, 29, 39-44.
38 Sharma V, Sharma A, Kansal L (2010). The effect of oral administration of Allium sativum extracts on lead nitrate induced toxicity in male mice. Food and Chemical Toxicol, 48, 928-36.   DOI
39 Reitman S, Franckal S (1979). A colorimetric method for the determination of serum glutathione oxaloacetate and glutamic- pyruvic transaminase. J Clin Pathol, 28, 481-90.
40 Sadashivam S, Manickam A (2004). Phenolics. Biochemical Methods. New age publishers, (New Delhi), 193-4.
41 Sharma V, Paliwal R, Pracheta, et al (2011). Phytochemical analysis and evaluation of antioxidant activities of hydroethanolic extracts of Moringa oleifera lam pods. J Pharm Res, 4, 554-7.
42 Sen S, Makkar HPS, Becker K (1998). Alfalfa saponins and their implication in animal nutrition. J Agri Food Chem, 46, 131-40.   DOI
43 Shyamal S, Latha PG, Shine VJ, et al (2006). Hepatoprotective effects of Pittosporum neelgherrense Wight&Arn., a popular Indian ethnomedicine. J Ethnopharmacol, 107, 151-5.   DOI
44 Singh RP, Padmanathi B, Rao AR, (2000). Modulatory influence of Adhatoda vesica (Justicia adhatoda) leaf extract on the enzymes of xenobiotic metabolism antioxidant status and lipid peroxidation in mice. Mol Cell Biochem, 213, 99-109.   DOI
45 Song LL, Steven R, Lantvit MD, et al (2000). Chemoprevention of DMBA-induced mammary carcinogenesis: Relationship between induction of phase II enzymes, effects on DMBA induced hemoglobin adducts and decrease in mammary tumor multiplicity. Polycyclic Aromatic Comp, 18, 193-210.   DOI
46 Sporn MB, Suh N (2000). Chemoprevention of cancer. Carcinogenesis, 21, 525-30.   DOI