• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.105-113
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• 2019

Although siderophore compounds are mainly biosynthesized as a response to iron deficiency in the environment, they also bind with other metals. A few studies have been conducted on the impact of heavy metals on the siderophore-mediated iron uptake by microbiome. Here, we investigated siderophore production by a variety of rhizosphere fungi under different concentrations of $Zn^{2+}$ ion. These strains were specifically isolated from the rhizosphere of Panax ginseng (Korean ginseng). The siderophore production of isolated fungi was investigated with chrome azurol S (CAS) assay liquid media amended with different concentrations of $Zn^{2+}$ (50 to $250{\mu}g/ml$). The percentage of siderophore units was quantified using the ultra-violet (UV) irradiation method. The results indicated that high concentrations of $Zn^{2+}$ ion increase the production of siderophore in iron-limited cultures. Maximum siderophore production by the fungal strains was detected at $Zn^{2+}$ ion concentration of $150{\mu}g/ml$ except for Mortierella sp., which had the highest siderophore production at $200{\mu}g/ml$. One potent siderophore-producing strain (Penicillium sp. JJHO) was strongly influenced by the presence of $Zn^{2+}$ ions and showed high identity to P. commune (100% using 18S-rRNA sequencing). The purified siderophores of the Penicillium sp. JJHO strain were chemically identified using UV, Fourier-transform infrared spectroscopy (FTIR), and matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) spectra.

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.179-183
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• 2013

Marine-derived microbes have yielded a variety of metabolites so far. In the course of the project to find metabolites from marine microbes, an isolate of Aspergillus protuberus (SF 5767) was selected for chemical investigation. A large scale culture of this strain in PDA media was extracted with an organic solvent and the extract was fractionated by silica gel column chromatography. Repeated reverse phase HPLC of the fractions led to the isolation of three metabolites. Their chemical structures were elucidated as deoxybrevianamide E (1), brevianamide V (2), and ergosterol peroxide (3) on the basis of spectroscopic data including MS, NMR, and UV. To the best of our knowledge, chemical investigation of A. protuberus was conducted for the first time in this study.

• Proceedings of the Korea Water Resources Association Conference
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• 2009.05a
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• pp.2190-2199
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• 2009

Nihombashi is located in the central area of Tokyo, Japan. Tokyo has been the capital in Japan since the Edo period, which started approximately 400 years ago, and has accepted a variety of cultures, human resources, businesses for the last 400 years. This has resulted in building up the present prosperity. The Sumida River, one of the symbols of Tokyo and its tributaries including the Kanda River and the Nihombashi River, flows through the Nihombashi district. The river and tributaries used to benefit to the City of Edo. Due to the economic development and the industrial growth in Tokyo, however, they were polluted and lost their functions. In 1960s, approximately 40 years ago, the Sumida River became so dirty that local citizens kept away from it. The Nihombashi River was covered with an expressway, which was obscuring the river view. Since 1970s, local communities have proposed to rehabilitate rivers in Tokyo successively, and have proceeded with measures for river floods, improvement of sewage systems and construction of water purification facilities. Consequently, the quality of the river water was considerably improved in 1990. The stagnant rivers were turned into ones that local citizens were physically able to come close by. Today, restoring of the environment and the appearance of the city in the old days, Nihombashi district has been proposed as a model city of the future, which is alive with history and culture and harmonizing with rivers. The concept is "To Create, To Reserve, To Restore." This paper introduces a case study of the urban development, in which the local communities and public authorities collaborated with and proposed a brand-new style of the urban city harmonizing with the environment.

• Journal of Distribution Science
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• v.15 no.1
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• pp.31-42
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• 2017

Purpose - The multicultural society is a society where a variety of ethnic groups and cultures co-exist. Regarding Korean multicultural society, the public interest in the multicultural society and its problems are on a growing trend due to the increasing number of the multicultural families by international marriage and the foreign immigrant workers. Research design, data, and methodology - Models of the multicultural society policies have been divided into those of assimilation and multiculturalism, while they have been materialized into the models of discriminative exclusivism, assimilation and multiculturalism. Most countries are aiming at the model of either the multiculturalism or the assimilation focused on national managerial situations. Results - In the case of Europe where the multicultural society had been formed earlier than Korea, Islamic immigrants have been politically accepted in order for Europe to overcome the problem of population decrease caused by its low birth-rate. Also, in the case of the United States. Conclusions - Korean multicultural society policies are characterized mostly by supporting the multicultural families of international marriage. In this study, it is intended to present the characteristics of diversified immigrants and the possible directions of the polices on immigrant youth in accordance with each country's managerial policy.

• Korean Institute of Interior Design Journal
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• v.23 no.4
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• pp.94-102
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• 2014

Nowadays when the social and cultural paradigm is changing, the incomplete space is becoming a matter of controversy. In order to figure out the solutions to it, are being held a variety of spatial discourses for spatial essence and meaning to be cleared. Accordingly, this study has tried to seek for any probability to interpret the ontology shown at any traditional space on the ground of Heidegger's Ontological Thinking Structure which has a considerable impact on Modern Space, whose conclusions are the followings. First, Heidegger's ontological space theory, which provided a foundation of Placeness concept, includes not only the character of interdisciplinary learning among philosophy, arts and any related studies but also that of mutual oriental and occidental cultures. Second, between the thoughts of Heidegger and Lao-tzu are considerable similarities from the methodical viewpoint that materializes the meaning of existence as an essence. Third, for a convenient interpretation, the ontological spatial concept of Lao-tzu's philosophy shown at traditional spaces have been categorized into Typology-Incident, Morphology-situation and Topology-meaning generation with Schultz's Existential Spatial Concept based on Heidegger's Ontology as a medium. In particular, the meaning generation which materializes the placeness has the trait of being clarified as the product of interactions between incidents and situations.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.584-591
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• 2009

Adventitious root culture was established in the Jawahar variety of Withania somnifera using MS basal medium supplemented with 0.5 (mg/l) IAA and 2.0 (mg/l) IBA. Root tips from germinated seedlings, MS0 maintained plants and adventitious roots were maintained in suspension medium (1/2 MS basal medium supplemented with 3% sucrose) for a period of 1 to 6 months. The weight gain in roots was noted and the withanolides were extracted from the dry roots using solvents petroleum ether, 50% ethanol and chloroform. The withanolides in the chloroform fractions of all root samples analyzed were compared using thin layer chromatographic analysis. Withanolide content in adventitious root sample was found to be superior compared to other roots at any given point of time during the 6month growth period.HPLC analysis of in vitro adventitious roots showed the presence of a new compound.

• Journal of the Korean housing association
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• v.20 no.5
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• pp.93-102
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• 2009

Housing is a cultural tool which enables one to see through a certain generation, and the types of housing in a specific cultural area are both physical and spiritual results formed by life activities. Therefore, housing, the center area for the human life, is a bowl, putting various meanings of life into the complicated environment. Its formal and spacial variety is a result of interaction in human life. This study analyzes fundamental problems and characteristics of asian traditional housing by comparing those in city of Seoul and Shang-hai. From the results of this study, many differences have been found in traditional housing in Seoul and Shanghai despite of high similarity m culture and ideology. This results from a same Confucian culture that accepts the feng shui theory which has evolved through different environments. Therefore, this study aims to understand housing cultures in two cities by analysing the attributes of housing data from selected traditional housing types in Seoul and Shanghai. Also, issues of fundamental characteristics of the two cities through the development of residential environment are studied to provide important data for the future development of housing environment of both cities.

• Mycobiology
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• v.37 no.1
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• pp.43-47
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• 2009

The levels of ergothioneine (ERG), which have been shown to act as an excellent antioxidant, were determined in both fruiting bodies and mycelia of various mushroom species. We found that ERG accumulated at different levels in fruiting bodies of mushrooms and showed up to a 92.3-fold difference between mushrooms. We also found that ERG accumulated at higher levels in mycelia than in fruiting bodies of economically important mushroom species such as Ganoderma neo-japonicum, G. applanatum and Paecilomyces tenuipes. The addition of 2 mM methionine (Met) to mycelial culture medium increased the ERG contents in most mushroom species tested, indicating that Met is a good additive to enhance the ERG levels in a variety of mushroom species. Taking these results into consideration, we suggest that the addition of Met to the mycelial culture medium is an efficient way to enhance the antioxidant properties in economically important mushroom species.

• ALGAE
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• v.18 no.4
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• pp.239-253
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• 2003

The morphotaxonomic characters of the genus Spirogyra were investigated to clarify their taxonomic category and the variation range among species on the basis of comparative morphological and numerical analyses by unialgal cultures and field samples collected from various freshwater habitats in Korea. 25 characters selected on morphological feature of the species were examined on 568 individuals for morphological comparisons and numerical analyses. Width, length and their length/width ratio of vegetative cell, shape of septum, chloroplast number, maximum width, width, length and their length/width ratio of female gametangium, length of male gametangium, size and shape of zygospore, and cell wall ornamentation of the spore showed a comparatively high vector in principal component analysis. In cluster analysis, 15 taxa analysed were divided into 8 major groups by the average taxonomic distance 1.0 level. Considering the morphology and numerical analysis, Spirogyra crassoidea could not be recognized as an independent species, therefore it was treated as a variety of S. ellipsospora. S. koreana (nom. invalidum), recognized as a new taxon, is under the investigation for its clear taxonomic category.

• YAKHAK HOEJI
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• v.45 no.4
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• pp.419-425
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• 2001

The neuronal cell death induced by excess glutamate (Glu) has been implicated in many acute and chronic neurodegenerative diseases including cerebral ischemia. Glu-induced elevation of intra-cellular $Ca^{2+}$ plays a critical role in the excitotoxicity, partly through the activation of a variety of $Ca^{2+}$ dependent enzymes. In the present study, we investigated the Glu-induced modulation of $Ca^{2+}$/calmodulin-dependent protein kinase IV (CaMK IV), a multifunctional enzyme abundantly present in the nuclei of neurons. The exposure of cultured rat cortical neurons to $100{\mu}$M Glu for 3 min dramatically increased CaMK IV activity up to 4.5-fold of the control-treated enzyme activity. The activation was very rapid, reaching peak at 3 min and then declined gradually. Under the same experimental conditions, time-dependent acute and delayed neuronal cell death was observed. Immunoblot analyses using specific antibodies showed that the expressions of CaMK IV and $CaMKK_{\alpha}$ were time-dependently modulated by Glu. Taken together, these results imply that the modulation of CaMK IV activity by Glu may be involved in the cascade of events resulting in neuronal cell death in cortical cultures.