• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.4
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• pp.213-217
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• 1978

Henri-Michaelis-Menten model for predicting the behavior of culture of Brevibacterium flavum under carbon limiting condition has been evaluated on a chemostat growing in the presence of the phenol derivatives: guaiacol, o-vanillin and vanillin. It is found that Henri-Michaelis-Menten model could be applicable to the evaluation of the growth rate of cells in the phenol derivatives. The marked enhancing abilities of the cells treated by the phenol derivatives during fermentation were ascribed to the formation of charge-transfer complex between the phenol-derivatives and oxygen which supplies oxygen effectively to the fermentation system.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.629-630
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• 2005

• Korean Journal of Pharmacognosy
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• v.19 no.3
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• pp.188-192
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• 1988

An aromatic hydrocarbon, $mp\;79{\sim}80^{\circ},\;C_{11}H_{12}O_5$, was first isolated from Phyllostachys sp. and identified as 1, 4-benzenedicarboxylic and 2'-hydroxyethylmethyl ester. Five compounds isolated after alkali hydrolysis were identified as vanillin, 4-hydroxyacetophenone, terephthalic acid, 4-hydroxybenzaldehyde and friedelin.

• Archives of Pharmacal Research
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• v.24 no.4
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• pp.312-315
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• 2001

Repeated column chromatographic separation of the $CH_{2}Cl_{2}$ extract of Artemisia stolonofera (Asteraceae) led to the isolation of a triterpene (I), a sesquiterpene (II), two aromatic compounds (III and IV) and a benzoquinone (V). Their structures were determined by spectroscopic means to be simiarenol (I), (1S,7S)-1 $\beta$-hydroxygermacra-4(15),5, 10(14)-triene (II), 3'-methoxy-4'-hydroxy-trans-cinnamaldehyde (III), vanillin(IV) and 2,6-dimethoxy-1,4-benzoquinone (V), respectively. Among these products, compound V showed significant cytotoxicity against five human tumor cell lines in vitro, A549 (non small cell lung adenocarcinoma), SK-OV-3 (ovarian), SK-MEL-2 (skin melanoma), XF498 (CNS) and HCT15 (colon) with ED_{50}$ values ranging from 1.33~4.22${\mu}g/ml$.

• Korean Journal of Acupuncture
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• v.26 no.4
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• pp.211-223
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• 2009

초임계추출법과 수증기증류법을 이용하여 황련해독탕의 정유성분을 추출하여 성분 패턴을 비교하였다. 이때 초임 계추출조건은 압력 200 atm, $45^{\circ}C$ 그리고 추출시간 25분 이었다. 이때의 추출된 성분의 93.9%인 37개의 성분을 가스크로마토그래피/질량분석기로 확인하였다. 주요성분으로는 tetradecenoic acid (11.7%), Vanillin (5.9%), dl-Limonene (5.5%) 및 Eicosane (4.6%)으로 나타났다. 수증기증류법으로 추출한 정유에서는 34개 성분을 확인할수 있었다. 주요성분으로는 tetradecenoic acid (8.9%), Vanillin (5.8%)및 Eicosane (4.7%)를 확인 할 수 있었다. 또한 구강내의 12균주를 이용하여 항균효과를 측정하여 최소엑제농도(MIC)와 사멸농도(MBC) 0.025 - 12.8 mg/ml와 0.05 - 12.8 mg/ml 각각 나타났다.

• Corrosion Science and Technology
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• v.21 no.4
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• pp.325-325
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• 2022

• ALGAE
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• v.31 no.4
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• pp.391-401
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• 2016

To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only $1-3{\times}10^5cells$ of P. minimum and A. minutum, $10^4cells$ of P. micans, and $10^3cells$ of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.

• Analytical Science and Technology
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• v.24 no.6
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• pp.421-428
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• 2011

In this study, headspace disk type monolithic material sorptive extraction (HS-MMSE) was developed, validated and applied to the analysis of volatile aroma compounds from vanilla perfume by gas chromatography -mass spectrometry (GC/MS). HS-MMSE uses monolithic material (MonoTrap) based on silica bonded with octadecyl silane (ODS) and activated carbon as a sorbent. Aroma compounds was adsorbed onto the MonoTrap in headspace and extracted by only 100 ${\mu}L$ of solvent. Total 12 volatile compounds from vanilla perfume were successfully analyzed using HS-MMSE. The influence of extractive parameters was investigated and optimized, using benzyl acetate, linalyl acetate, vanillin, ethyl vanillin as target compounds. Under the optimum condition, the limit of detection (S/N = 3) and the limit of quantification (S/N = 10) of proposed method for the target compounds were obtained within the range of 8.35~13.76 ng and 27.82~45.88 ng, respectively. The method showed good linearity with correlation coefficient more than 0.9888, satisfactory recovery and reproducibility. These results showed that HS-MMSE using disk type MonoTrap is a new promising technique for the analysis of volatile aroma compounds from vanilla perfume.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1978.04a
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• pp.97.1-97
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• 1978

발효에 guaicol, vanillin 및 O-V-anillin phenol 등의 유도체를 처리한 결과, yeast, Bacillus subt-ilis, Brevibacterium flavum, Pseudomonas ovalis 등의 mass, 호흡량, 성장속도 등에 미치는 영향이 크므로 발효공학에 이들 phenol 유도체를 이용하면 생산성을 향상시킬 수 있을 것으로 예상되었다.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.203-203
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• 2004