• 디지털융복합연구
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• 제16권11호
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• pp.447-454
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• 2018

팝아트는 대중문화와 순수예술의 경계를 허무는 시대 문화적 현상을 표현함으로써 대중과 소통을 유도하고자 하였다. 도자 분야에도 팝아트 표현 기법의 특성을 활용하여 표현 영역을 확대하고 있다. 본 연구는 팝아트의 이론적 고찰을 통해 역사적 배경과 작가들의 작품에 나타난 팝아트 양식의 소재와 모티브의 표현특성을 규명하고 그 표현특성이 도자작품에 어떻게 활용되었는지 분석하였다. 작품분석결과 대중문화속의 일상적인 대상의 이미지를 밝고 화려한 색채, 실크스크린, 레터링, 포토몽타주, 만화적 표현, 오브제, 콜라주, 데쿠파주 등의 기법들을 다양하게 표현하였으며, 도자작품들이 대중 속에서 예술문화를 실현하고 보다 대중적인 소재와 심리를 표현하는 것에 가치를 두었다 사료되며 앞으로 도자예술이 팝아트 표현기법 위주의 재현이 아닌 예술적 가치를 재해석한 창의적 작품으로 창출되어 대중과 소통하는 문화예술로 더욱 발전되기를 기대해 본다.

• Biomolecules & Therapeutics
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• 제31권1호
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• pp.82-88
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• 2023

Genomic analysis indicated that the genome of Drosophila melanogaster contains more than 80 cytochrome P450 genes. To date, the enzymatic activity of these P450s has not been extensively studied. Here, the biochemical properties of CYP6A8 were characterized. CYP6A8 was cloned into the pCW vector, and its recombinant enzyme was expressed in Escherichia coli and purified using Ni²⁺-nitrilotriacetate affinity chromatography. Its expression level was approximately 130 nmol per liter of culture. Purified CYP6A8 exhibited a low-spin state in the absolute spectra of the ferric forms. Binding titration analysis indicated that lauric acid and capric acid produced type I spectral changes, with K_d values 28 ± 4 and 144 ± 20 µM, respectively. Ultra-performance liquid chromatography-mass spectrometry analysis showed that the oxidation reaction of lauric acid produced (ω-1)-hydroxylated lauric acid as a major product and ω-hydroxy-lauric acid as a minor product. Steady-state kinetic analysis of lauric acid hydroxylation yielded a k_cat value of 0.038 ± 0.002 min^-1 and a K_m value of 10 ± 2 µM. In addition, capric acid hydroxylation of CYP6A8 yielded kinetic parameters with a k_cat value of 0.135 ± 0.007 min^-1 and a K_m value of 21 ± 4 µM. Because of the importance of various lipids as carbon sources, the metabolic analysis of fatty acids using CYP6A8 in this study can provide an understanding of the biochemical roles of P450 enzymes in many insects, including Drosophila melanogaster.

• 복식문화연구
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• 제8권3호
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• pp.436-499
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• 2000

The purpose of this study is to be inquired social and cultural significance of the Grotesque phenomenon in the late 1990's make-up, as well as its experimentalism and avantgardism. The Grotesque is the aesthetic concept, which is composed of ambivalent structure of humor and horror, interest and disgust through join of heterogenous factors, distortion, extreme and overstatement in the patterns and materials of make-up. The Grotesque in the modern make-up is represented in the make-up advertisements and collections. In this paper, the author classified the Grotesque phenomenon with the Kitsch, the Machine, the Animal and the Devil, focused on heterogenous factors. The results are as follows. 1. The Kitsch in the make-up uses not cosmetics but imitations, ready-made goods, and ordinary materials. It expresses modern culture's laziness through shocking self-expression. 2. The Machine in the make-up means silver or white color's make-up, and represents cyber period's arrival through contrary irony, loss of the human value and the personifications of machine. At the same time, it is understood as the curing of human alinetion. 3. The Animal in the make-up utilizes the wild animal's horn, the reptiles'sputum, and the legend's animal. It points out a human false virtual image, and overturns make-up's traditional aesthetic concept. 4. The Devil in the make-up shows vampire's cuspid, demon's horn, artificial nail, and black color's make-up. It raises social irrationality and discrepancy of capitalism, and expresses paradoxically the nature of human life. The Devil in the make-up expresses the creator's innovation, anxiety of fin-de-siecle, and simultaneously criticizes and cures confused reality. It shows also avantgarde implementation of dissolution of traditional aesthetic concept and acceptance ugliness.

• 복식
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• 제50권7호
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• pp.75-96
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• 2000

Modern clothing is approached in the level of art, and it becomes the means of expressing individuality in modern society uniformed by the development of information society. In this modern society, modern men need the recovery of humanity and expectations of handcrafted skills for the succession of tradition culture. Prior to expressing individuality, we should examine our traditional culture and combine it with western culture. Quit started to be used for practical purpose such as life items, but it is expanded to the fields of art. Used in dress and its ornaments design, quilt is often applied to the addition of aesthetic factors or cubic material feelings by transforming its warming effect. Hereby, this study has a purpose to create high value added modern dross and its ornaments design by expressing the modern clothing is approached in the level of art, and it becomes the means of expressing individuality roe-dimensional characteristics of quilt, and applying the color combination and the surface of butterfly wings to dress and its ornaments design. The following are the results of this study. 1. The concept of quilt started for practical purposes, but it is expanded to decoration in modern times, and it is confirmed that quilt can be variously applied to handcrafted modern design. 2. Quilt removes the plane character of textile and it riches the three-dimensional material of dress and its ornaments. Applying these characteristics, the expression of transparent wings were possible with 3 transparent layers of textile. 3. The spledid color of butterfly wings are expressed by coloring oganza and felt, and the various colors of felt showed rich color gradation. 4. The form and pattern of butterfly wings are applied as modeling form and line, and the transformation. repetition and expansion of unit forms determined the form of quilting lines. By designing the characteristics of back wings for the composition line of clothing, the form characteristics of a motif could be emphasized. 5. By using felt, oganza, Damdam yarn and ostritch feathers in expressing butterfly wings, the warm material of Linbun is felt, and the tip hair of wings are expressed by croche techniques using Damdam yarn.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.116-120
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• 1991

The transformant Bacillus subtilis ED213 carrying the pSO100 which cloned the cdd gene encoding cytidine deaminase (cytidine /2'-deoxycytidine aminohydrolase, EC 3.5.4.5, CDase) originated from wild type B. subtilis was cultivated in Spizizen minimal medium (SMM). To overcome poor expression of the cdd gene in SMM medium, the medium compositions and growth conditions were optimized. The optimized medium compositions and growth conditions were cytidine concentration of 80 mg/l, glycerol of 25 g/l, and $(NH_4)_2SO_4$ of 10 g/l, along with $37^{\circ}C$ and pH 7.0. The intracellular CDase production was increased 3 times from 1,000 unit/ml to 3,200 unit/ml, and extracellular CDase also increased from nearly undetectable amounts to 1,500 unit/ml. The cytidine concentration was signified as the most critical compositional factor for overproduction of CDase by increasing the cell density mainly in culture broth. The plasmids were more stable in cells that were grown in original SMM medium with stability of 90% compared to those grown in optimized SMM medium with stability of 80% after 48 hours cultivation. The most active amplification of plasmid was occurred in the logarithmic phase, which showed a value around four times higher than the initial copy number. In the exponential phase, the CDase production was closely related to the plasmid copy number along with the cell density. However, it was not accorded with cell density at the stationary phase.

• 복식문화연구
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• 제17권6호
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• pp.1083-1098
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• 2009

The purpose of the study is to revaluate the new artistic values of Suprematism that immensely influenced the Russian culture in the beginning of the 20th century. Also through studying the relationship between people and costume within works of Malevich, who wanted to portray humanity through his art works, this study will research on how Suprematism not just stood as Russian Avant-garde art but significantly contributed to art and costume expression. The study methods are as follows. First, consider the formative background of Suprematism style of arts. Second, study of the development of Suprematism art through both Suprematism art and Non-objective art. Third, study how Suprematism style of arts is portrayed in art costume through works of Malevich. The results of the study are as follows. The Russian Suprematism art costume strictly originated from the Russian Suprematism style of arts. Suprematism simplified any reproductive element of fine art with a creative energy, which reflects the statement of Malevich that said, "The object does not exist, it only stands as a symbol." In other words, Suprematism abolished ordinary response to basic environment, and created a much more serious reality than the innate reality of the environment. When applied to art costume, such style sought after geometrical simplicity in shape and Neo-Premitivism trait in color. This trend is also expressed in 21st century fashion in new forms of Minimalism or Futurism. In this respect, Suprematism still holds an artistic value in a novel form even after a century since the beginning of 20th century when Malevich first presented Suprematism.

• 지역과문화
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• 제8권3호
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• pp.27-48
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• 2021

近年来, 中国的旅游演艺进行了一系列新项目的落成, 使旅游演艺产业的票房增加, 经济收入增高, 同时带动了旅游演艺的品牌形成, 而且拥有了一定的良好口碑, 对地域的文化传播, 经济发展都有一定的推动作用, 其中大型情景体验剧就是重点项目之一。大型情景体验剧是一种以模拟真实环境空间设计为空间, 使观众在视觉, 听觉, 嗅觉, 味觉和触觉等多位感官上进行主动式体验, 互动性极强的新戏剧形式。大型情景体验剧根据中国的传统文化, 地域文化, 流传已久的故事进行了改编, 又结合了灯光, 音效, 特效, 3D效果等高科技, 使观众的体验更加真实。《又见平遥》作为中国第一部大型室内情景体验剧, 以新的表现形式和创作手段来体现山西文化的博大精深。以情景体验剧的形式, 使观众更加直观地感受到了"山西人"的"山西情"和"山西德", 推动山西旅游业的发展, 带动山西地区的经济, 逐渐形成了完整的产业链。但也有剧情连接不顺及游客管理不当等局限, 可以通过更多的观众互动及指引完善演出效果。因此可以看出, 大型情景体验剧对地域在传统文化, 价值观的传播上, 旅游产业的发展上, 地域的品牌特色形成以及经济发展上都有很大的推动作用。通过这些, 可以看出大型情景体验剧对实景演出的发展具有创新思维内容, 逐渐形成产业链闭环以及拓宽宣传渠道等启示作用。

• 지능정보연구
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• 제20권3호
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• pp.45-58
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• 2014

몰입은 관람객이 콘텐츠를 관람할 때 관람객들이 콘텐츠에 몰두하고 있는 심리적 상태를 의미하는 것으로, 관람객의 몰입경험은 콘텐츠의 만족도에 긍정적인 영향을 미친다. 따라서 공연 같은 콘텐츠를 제공하는 기업들은 콘텐츠의 흥행을 위해 관람객의 몰입도를 측정하는 것은 매우 중요하다. 설문 등의 표본조사 방법을 통해 관람객의 몰입도를 측정 연구는 방송분야 등 에서 널리 사용되고 있다. 이러한 몰입도 측정방법은 콘텐츠 관람 이후 설문을 실시하기 때문에 몰입도를 실시간으로 측정할 수 없을 뿐만 아니라 몰입도 측정의 정확성이 저하되는 문제 등이 있다. 이러한 문제를 해결하기 위하여 생리적 반응이나 얼굴 표정 분석, 그리고 움직임 관찰 방법 등을 이용하여 몰입도를 측정하는 연구가 수행되고 있다. 생체 신호를 이용하여 몰입도를 측정하는 연구의 경우, 1인을 대상으로 생체신호를 측정할 뿐만 아니라, 많은 데이터 처리 시간과 비용이 소모되는 단점이 있어 많은 관람객이 관람하는 공연장에 적용하기에는 한계가 있다. 얼굴 표정인식 통해 몰입도를 측정하는 경우도 1인을 대상으로 하고 있으며, 밝은 조명의 실험실 환경에서만 가능하다는 단점이 존재한다. 또한 관람객들의 움직인 동기화를 이용하여 몰입도를 특정한 연구는 다중관객을 대상으로 하였지만, 이는 실험실 환경에 한정하여 적용된 사례이다. 따라서 본 연구에서는 공연장, 시사회관 등 많은 관람객들이 콘텐츠를 관람하는 실제 환경에서 다중관람객이 다중몰입도의 정량적 평가를 위한 시스템을 설계하고 개발하였다. 제안된 시스템은 외부장치, 서버, 내부장치 등의 3부분으로 구성되어 있다. 서울시 마포구 상암동에 위치한 DMC 홍보관에 상설 전시장으로 운영하고 있으며, 관람객들을 대상으로 데이터를 획득하고 있다. 제안하고 있는 시스템을 활용하면 콘텐츠의 어느 구간에서 관객들이 몰입을 하고 있는지, 어느 구간에서 몰입을 하고 있지 못한지 분석가능하기 때문에, 향후 콘텐츠 제작 및 마케팅에 유용하게 활용할 수 있을 것으로 기대된다.

• Imaging Science in Dentistry
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• 제35권4호
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• pp.191-198
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• 2005

Purpose: To characterize the effects of 2-deoxy-D-glucose (2DG) and quercetin (QCT) on cytokine secretion of IL-6, $TGF-\beta$ and gene expression of Col I in irradiated MC3T3-E1 cells Materials and Methods: The MC3T3-El cells were cultured in an a-MEM supplemented with 5mM 2DG or 10mM QCT and then the cells were incubated 12h before irradiation with 2, 4, 6, and 8Gy X-ray using a linear accelerator delivered at a dose rate of 1.5Gy/min. Level of IL-6 and $TGF-\beta$ was determined by ELISA. Also expression of Col I was examined by RT-PCR. Results: In accordance with the radiation dose, the amount of $TGF-\beta$ was not different in RA + QCT, but it showed a peak value in control and RA + 2DG at 4Gy on the 3rd day. However, all groups showed a decreasing tendency dose-dependently in RA+QCT on the 7th day (p<0.01). In accordance with the radiation dose, the amount of IL-6 increased dose-dependently in all groups on the 3rd day. On the 7th and 21st day, all groups showed peak values at 4Gy. RA+QCT showed a slightly increased amount of IL-6 at 2Gy, but it showed a slightly decreased amount at 4, 6, and 8Gy. In accordance with the period of culture after irradiation, the expression of Col I increased dose-dependently in RA+QCT. Conclusion: The result showed that QCT acted as radiosensitizer in the secretion of $TGF-\beta$ and gene expression of Col I during differentiation in irradiated MC3T3-E1 cells at the cellular level.

• 한국미생물·생명공학회지
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• 제26권1호
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• pp.68-75
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• 1998

A. niger의 GOD(Glucose Oxidase) 대량생산과 효율적인 분비를 protein의 대량생산에 많이 사용되는 strain인 S. cerevisiae에서 시도하였다. S. cerevisiae의 ADH1과 GAL 10 promotor, 그리고 ${alpha}$-MF signal sequence와 A. oryzae의 ${alpha}$-amylase signal sequence 및 S. cerevisiae의 GAL7과 A. niger의 GOD terminator를 이용하여 4개의 expression vector를 합성한 후 S. cerevisiae 2805에 auxotroph 방법으로 형질변환시켰다. 변이체들을 배양하여 세포내와 세포외의 GOD활성도를 분석한 결과 GAL 10 promotor가 삽입된 pGAL변이체들이 ADH1 promotor가 삽입된 pADH 변이체들 보다 GOD 생산성이 높았다. GAL 10 promotor와 A. oryzae의 ${alpha}$-amylase signal sequence가 삽입된 pGALGO2에서 115시간 배양시 GOD의 생산이 가장 높았다($GOD_{total}$: 10.3 unit/mL, $GOD_{ex}$: 8.7 unit/mL). 이 수치는 같은 promotor인 GAL 10 promotor와 ${alpha}$-MF signal sequence가 삽입된 pGALGO1보다 3배정도 높다. 이 결과는 ADH 1 promotor를 사용하였을 경우에도 일치하였다. 또한 A. oryzae의 ${alpha}$-amylase signal sequence가 S. cerevisiae의 ${alpha}$-MF signal sequence보다 GOD를 더 효과적으로 분비시켰다. 상기 결과로 미루어 보면 signal sequence가 단백질의 분비 외에도 단백질 합성에도 많은 영향을 주는 것으로 추측된다. pGALGO1과 pGALGO2의 GOD분비효율은 각각 89%, 84%이었다. S. cerevisiae에서는 일반적으로 과당화가 일어나기 때문에 S. cerevisiae에서 합성된 재조합 GOD의 분자량은 250 kDa으로 A. niger의 GOD(170 kDa)보다 더 컸다.