• 한국산업정보학회논문지
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• 제11권5호
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• pp.150-162
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• 2006

본 연구에서는 물리적 제품을 판매하는 인터넷쇼핑몰이 판매제품을 생산하는 산업클러스터에 입지하였을 경우 얻을 수 있는 효익 요인과 경쟁전략 간의 관계를 분석하였다. 국내 주요 클러스터에 입지해 있는 인터넷쇼핑몰을 대상으로 한 설문조사에서 총 99개의 유효한 응답을 얻었다. 분석 결과, 생산자와의 정보공유, 고객확보, 신뢰증진, 업무프로세스 효율성, 부가가치 제공, 생산자 관리 등 6개의 효익요인이 추출되었다. 이어서 6개의 효익요인이 해당 인터넷쇼핑몰의 경쟁전략에 어떠한 영향을 미치는가를 알기위해 회귀분석이 수행되었으며, 그 결과 해당 쇼핑몰들이 생존하고 성장하기 위한 경쟁전략 또는 경쟁전략의 조합의 선택에 있어서 이 여섯 가지 효익요인이 큰 영향을 미친다는 사실이 나타났다.

• 신재생에너지
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• 제16권2호
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• pp.28-34
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• 2020

The South Korean government invested about 560 billion won in research and development in the wind power sector over 30 years from 1989 to 2019. In addition, the government has been setting up conditions for the country's wind industry to grow through various policies, including the RPS (Renewable Energy Portfolio Standard). As a result, the size of Korea's wind industry has grown to 1.1 trillion won in annual sales and there are 2,000 people working in the wind energy sector. While the domestic market is weak due to the small size of Korea and there are problems with the NIMBY, the government has continued to support technology developments and support market policies. Despite insufficient performance in overseas markets, the effect of the government is still significant on the growth of the wind industry. In particular, the government's R&D investment program, which focuses on enhancing companies' competitiveness, has spurred job creation in the wind industry and a stable research environment for researchers. In this study, we compared the differences between methods of investigating employment statistics in Korea and those of other countries. We also proposed effective investment measures for the government by analyzing the investment effects according to value chain and types of organization.

• Journal of Microbiology
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• 제39권4호
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• pp.279-285
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• 2001

Vibrio parahaemolyticus is a halophilic bacterium associated with seafood gastroenteritis. An unusual strain of Kanagawa-positive urease producing Vibrio parahaemolyticus O1:K1 was isolated from the environment and identified . A polymerase chain reaction assay revealed that this strain harbored both the tdh and the genes. The urease from this strain was studied. Maximum urease production was induced in LB medium containing 0.2% urea, 0.5% glucose, 2% NaCl and pH 5.5 with 6h of culti-vation at 37$\^{C}$ under aeration. Purification of urease was achieved by the process of whole cell lysate, 65% ammonium sulphate precipitation, DEAE-cellulose ion exchange column chromatography, Sepharose CL-6B gel filtration and oxirane activated Sepharose 6B-urea affinity chromatography with 203 fold purification and 2.2% yield. Analysis of the purified enzyme by SDS-PAGE demonstrated the presence of the subunits with a molecular weight of 85kDa, 59kDa, 41kDa and the molecular weight for the native enzyme by nondenaturing PAGE and gel filtration chromatography was 255kDa. The purified urease was stable at pH 7.5 and the opeimal pH in HEPES buffer was 8.0 The enzyme was stable at 60$\^{C}$ for 2 h with a residual activity of 32% . The addition of 10$\mu$M if NiCl$_2$maintained stability for 30 min. The Km value of the purified enzyme was 35.6 mM in urea substrate. The TD$\_$50/(median toxic dose) of the purified urease was 2.5$\mu\textrm{g}$/ml on human leukemia cells.

• Animal cells and systems
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• 제5권3호
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• pp.217-224
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• 2001

The present study examines the expression and regulation of gonadotropin-releasing hormone (GnRH) and its receptor (GnRH-R) mRNA levels during mouse ovarian development. A fully processed, mature GnRH mRNA together with intron-containing primary transcripts was expressed in the immature mouse ovary as determined by Northern blot analysis and reverse transcription-polymerase chain reaction (RT-PCR). The size of ovarian GnRH mRNA was similar to that of hypothalamus, but its amount was much lower than that in the hypothalamus. Quantitative RT-PCR procedure also revealed the expression of GnRH-R mRNA in the ovary, but the estimated amount was a thousand-fold lower than that in the pituitary gland. We also examined the regulation of ovarian GnRH and GnRH-R mRNA levels during the follicular development induced by pregnant mare's serum gonadotropin (PMSG) and/or human chorionic gonadotropin (hCG). Ovarian luteinizing hormone receptor (LH-R) mRNA was abruptly increased st 48 h after the PMSG administration and rapidly decreased to the basal level thereafter. Ovarian GnRH mRNA level was slightly decreased at 48 h after the PMSG administration, and then returned to the basal value. GnRH-R mRNA level began to increase at 24 h after the PMSG treatment, decreased below the uninduced basal level at 48 h, and gradually increased thereafter. HCG administration did not alter ovarian GnRH mRNA level, while it blocked the PMSG-induced increase in GnRH mRNA level. Taken together, the present study demonstrates that the expression of GnRH and GnRH-R mRNA are regulated by gonadotropin during follicular development, suggesting possible intragonadal paracrine roles of GnRH and GnRH-R in the mouse ovarian development.

• 대한전자공학회논문지TC
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• 제46권2호
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• pp.97-103
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• 2009

최근 802.11 무선 네트워크에서 TCP성능 향상을 위한 많은 연구가 이루어지고 있다. End-to-end 연결에서 TCP성능을 결정하는 가장 큰 요인으로는 hop-count와 RTT (Round Trip Time) 이다. 본 논문에서는 TCP의 성능을 향상하기 위해, hop-count와 RTT 변경 시 적절한 CWND값을 설정하여 TCP-CEV을 변경하는 기법을 설명한다. 본 논문에서 제안한 기법은 기본 TCP 구조를 따르는 대부분의 transport 프로토콜에 적합하고, 분석적인 과정을 통해 결과를 도출한다. 시뮬레이션을 통해 제안한 기법이 체인 토폴로지에서 12%, 격자 토폴로지에서 4.9% 이상 성능이 향상되었으며, TCP window가 특정 값에 수렴하는 것을 보인다.

• Genomics & Informatics
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• 제13권3호
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• pp.70-75
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• 2015

MicroRNAs (miRNAs) have been demonstrated to play an important role in carcinogenesis. Previous studies revealed that miRNAs are present in human plasma in a remarkably stable form that is protected from endogenous RNase activity. In this study, we measured the plasma expression levels of three miRNAs (miR-21, miR-27a, and miR-155) to investigate the usefulness of miRNAs for gastric cancer detection. We initially examined plasma miRNA expression levels in a screening cohort consisting of 15 patients with gastric cancer and 15 healthy controls from Korean population, using TaqMan quantitative real-time polymerase chain reaction. We observed that the expression level of miR-27a was significantly higher in patients with gastric cancer than in healthy controls, whereas the miR-21 and miR-155a expression levels were not significantly higher in the patients with gastric cancer. Therefore, we further validated the miR-27a expression level in 73 paired gastric cancer tissues and in a validation plasma cohort from 35 patients with gastric cancer and 35 healthy controls. In both the gastric cancer tissues and the validation plasma cohort, the miR-27a expression levels were significantly higher in patients with gastric cancer. Receiver-operator characteristic (ROC) analysis of the validation cohort, revealed an area under the ROC curve value of 0.70 with 75% sensitivity and 56% specificity in discriminating gastric cancer. Thus, the miR-27a expression level in plasma could be a useful biomarker for the diagnosis and/or prognosis of gastric cancer.

• The Korean Journal of Physiology and Pharmacology
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• 제6권5호
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• pp.255-260
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• 2002

The effects of intracellular and extracellular pH on the inwardly rectifying $K^+$ (IRK) channel of the bovine aortic endothelial cells (BAECs) were examined using whole-cell patch-clamp technique. The IRK current, efficiently blocked by $Ba^{2+}\;(200{\mu}M),$ is the most prominent membrane current in BAECs, which mainly determines the resting membrane potential. The expression of Kir2.1 was observed in BAECs using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Intracellular alkalinization, elicited by the extracellular substitution of NaCl with $NH_4Cl$ (30 mM), significantly augmented the amplitude of IRK current. On the contrary, the amplitude of IRK current was attenuated by the Na-acetate (30 mM)-induced intracellular acidification. The changes in extracellular pH also closely modulated the amplitude of IRK current, which was decreased to $40.2{\pm}1.3%$ of control upon switching the extracellular pH to 4.0 from 7.4. The extracellular pH value for half-maximal inhibition (pK) of IRK current was 5.11. These results demonstrate that the activity of IRK channel in BAECs, probably Kir2.1, was suppressed by proton at both sides of plasma membrane.

• 한국항해학회지
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• 제19권1호
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• pp.49-69
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• 1995

What is the national competitive power\ulcorner What are the determinants of national competitive advantage\ulcorner In order to find these problems, this study was initiated theoretically through the international trade theory and international managerial strategic theory. The flow of studies, in connection with these competitive advantage, come into industries competitive advantage from national competitive advantage. It is understood that the flow of studies is based on the change of paradigm about the international industry environment. Today, the international industry environment has been changed into vigorous situation with the new trade system such as globalization, UR negotiation, WTO system instead of GATT. Among these environments, all enterprises in any industries should try to enhance their benefit, income profits and develop their management strategy, because it is essential for them to keep and make a profit in order to be competitive against all other rivaling enterprises. According to researchers and the ages of history, the concept and determinants of competitive advantage have been dissimiliar as followings. 1) Theory of absolute advantage : Absolute Product Cost. 2) Theory of comparative advantage : Comparative Product Cost. 3) Theory of reciprocal demand : the Reciprocal Demand Structure. 4) Theory of Hechsher-Ohlin : Factor Endowment. 5) Paradox of Leontief : the Quality of Factor Endowment. 6) Theory of technological gap : R & D. 7) Technological gap model : The Technological Gap. 8) Theory of the product life cycle : Product Life Cycle. 9) Christensen's study : Firm's character & manager's character. 10) Monopolistic Advantage Theories : Monopolistic Advantage 11) Electic theory : Ownership, location and internalization specific advantages. 12) Theory of value chain : Cost advantage, Differentiation, Focus. However all these theories have the limit of illustration regarding today's competitive situation, the Porter's theory shows well the determinants of competitive advantage with competitive strategy. Therefore, the analysis of the competitive advantage for maritime industry should be studied by the determinants of competitive advantage of nations

• Journal of Preventive Medicine and Public Health
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• 제45권4호
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• pp.251-258
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• 2012

Objectives: The purpose of this paper was to elucidate the potential methylation levels of adjacent normal and cancer tissues by comparing them with normal colorectal tissues, and to describe the correlations between the methylation and clinical parameters in Korean colorectal cancer (CRC) patients. Methods: Hypermethylation profiles of nine genes (RASSF1, APC, $p16^{INK4a}$, Twist1, E-cadherin, TIMP3, Smad4, COX2, and ABCB1) were examined with 100 sets of cancer tissues and 14 normal colorectal tissues. We determined the hypermethylation at a given level by a percent of methylation ratio value of 10 using quantitative methylation real-time polymerase chain reaction. Results: Nine genes' hypermethylation levels in Korean CRC patient tissues were increased more higher than normal colorectal tissues. However, the amounts of $p16^{INK4a}$ and E-cadherin gene hypermethylation in normal and CRC tissues were not significantly different nor did TIMP3 gene hypermethylation in adjacent normal and cancer tissues differ significantly. The hypermethylation of TIMP3, Ecadherin, ABCB1, and COX2 genes among other genes were abundantly found in normal colorectal tissues. The hypermethylation of nine genes' methylation in cancer tissues was not significantly associated with any clinical parameters. In Cohen's kappa test, it was moderately observed that RASSF1 was related with E-cadherin, and Smad4 with ABCB1 and COX2. Conclusions: This study provides evidence for different hypermethylation patterns of cancer-associated genes in normal and CRC tissues, which may serve as useful information on CRC cancer progression.

• 화훼연구
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• 제18권3호
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• pp.201-206
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• 2010

심비디움속 유전자원 48품종에 대하여 RAPD와 URP를 이용하여 유전적 유연관계를 분석하였다. RAPD분석에는 10mer에 해당하는 random primer (Operon사) 80개를, URP는 20 mer에 해당하는 12종의 상용 primer를 이용하였다. 48 품종의 심비디움에는 34종의 동양 심비디움, 7종의 동서양란 교잡종, 7종의 서양 심비디움이 포함되어 있다. 선별된 41개의 random primer와 6개의 URP primer로부터 각각 407, 56개의 다형성 밴드를 획득하여 총 463개의 마커를 이용하였다. 이들 마커의 크기 범위는 0.4 kb 에서 1.5 kb 에 해당하였다. 유전적 유사도를 바탕으로 UPGMA clustering 프로그램을 이용하여 dendrogram을 작성하였는데 유전자원 48품종은 유사도 0.638 수준에서 총 4그룹으로 구분되었다.