• 제목/요약/키워드: Valine

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Streptomyces nigrifaciens GMT-4967이 생산하는 항생물질 MT-497의 분리와 화학적 특성 (Purification and Chemical Characterization of Antibiotic MT-497 Produced by Streptomyces nigrifaciens GMT-497)

  • 이영선;안순철;윤병대;민태익;이정형;이현선;안종석;민태익
    • 한국미생물·생명공학회지
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    • 제19권6호
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    • pp.604-609
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    • 1991
  • 토양에서 분리한 Streptomyces nigrifaciens GMT-497호무터 G(+) bacteria에만 강한 항균활성을 갖는 항생물질 MT-497을 용매추출, silica column chromatography와 재결정화를 통하여 분리 정제하였다. MT-497의 UV, 융점, 원소분석, IR spectrum, $^1H-NMR$과 구성아미노산 분석을 통해 actinocin chromophore와 threonine, proline, methyl valine, sarcosine, aspartic acid로 구성된 peptide을 갖는 actinomvcin계열의 항생물질로 동정하였다.

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무당개구리 간의 아미노산 성분에 관한 연구 (Studies on Amino Acids in the Frog Liver (Bombina orientalis Boulenger))

  • 박상윤;오석훈
    • 한국동물학회지
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    • 제3권2호
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    • pp.5-8
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    • 1960
  • Paper partition chromatography has been applied to estimate the amino acids in the liver of Bombina orientalis Boulenger. The results obtained are as follows : 1) The presence of cystine, serine, glycine, threonine, alanine, $\beta$-alanine, tyrosine, histidine, asparagine, tryptophane, methionine, valine, phenylalanine, leucine and an unknown substance has been demonstrated in the alcoholic extracts of the material. 2) The presence of glutamine, cystine, serine , glycine, arginine, threonine, histidine, alanine, methionine, proline, valine ,phenylalanine, leucine, and two unknown substances has been demonstrated in the acid-hydrolyzed liver. 3) Tyrosine, asparagine, and trypotphane have been demonstrated only in the alcoholic extract. 4) Proline, glutamine and arginine have been demonstrated only in the acid-jydrolyzed liver.

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p-Coumaroylamino Acids from Yeast-Elicited Ephedra distachya Cultures

  • Song, Kyung-Sik;Sankawa, Ushio;Ebizuka, Yutaka
    • Archives of Pharmacal Research
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    • 제17권1호
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    • pp.48-50
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    • 1994
  • Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.

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인삼박이 흰쥐의 성장과 체성분에 미치는 영향 (Effects of Ginseng-cake on Growth and Biochemical Components of Rats)

  • 이정실;김을상;김해중
    • 한국식품영양과학회지
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    • 제20권4호
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    • pp.329-336
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    • 1991
  • This study was carried out to investigate the effect of ginseng-cake on growth and biochemical components of rats. The content of crude protein in ginseng-cake was 17.2% as dry basis. Methionine, isoleuine and valine were limiting amino acids in order and arginine was 23.6% in total amino acids. Body weight gain, food efficiency ratio, organ weight and biochemical components in plasma were significantly lower in ginseng-cake based diet group than in control diet group and those of ginseng-cake diet group supplemented with methionine, isoleucine and valine were significantly increased than those of ginseng-cake based diet group, but 소id not reach to those of control diet group.

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대구알젓의 맛성분 제I보 : 대구알젓의 유리아미노산 (The Taste Conpounds of the Fermented Cod-roe, Gadus Macrocephlus - Changes of Free Amino Acids during the Fermentation of Cod-roe -)

  • 박재옥
    • 대한가정학회지
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    • 제20권4호
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    • pp.99-105
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    • 1982
  • Changes of free amino acids as taste compunds during the fermentation of cod-roe, Gadus marcrocephlus, were analyzed by a amino acid autoanalyzer. Leucine, lysine, valine, isoleucine and arginine were dominent amino acids in fresh cod-roe extracts, and the amounts of leucine (797.3mg%, on moisture and salt free base), lysine (577.7mg%) and valine(487.4mg%0 were 54.9% of total free amino acids. The contents of methionine, histidine, tyrosine and phenylanine were lower, and aspartic acid, glycine, threonine, glutamic acid and serine were detected trace amount. The free amino acids analyzed in this experiment were not changed in composition but changed in the amounts during 80 days of the fermentation. Total free amino acids were increased until 59 days of fermentation and than decreased gradually.

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A New Technique for Single Spore Isolation of Two Predacious Fungi Forming Constricting Ring

  • Singh, Kalika Prasad;Kumar, Dharmendra;Bandyopadhyay, Pinaki
    • Mycobiology
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    • 제32권4호
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    • pp.197-198
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    • 2004
  • A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at $29{\pm}1^{\circ}C$ for few days which yielded axenic culture of these fungi.

송류화분(松類花粉)의 아미노산(酸) 조성(組成) 및 화분발아(花粉發芽)에 따른 그 조성(組成)의 변화(變化) (On the Amino Acid Composition in the Pollen of Pines and Changes of the Composition during Germination of Pollen)

  • 홍성옥
    • 한국산림과학회지
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    • 제6권1호
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    • pp.43-48
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    • 1967
  • In the present experiment the amino acid composition in the pollen of Pinus taeda and Pinus rigida and methbolic changes of the composition occurring in germinating pollen were examined by the method of paper chromatography. The results obtained in this study are as follows. 1. The mature pollen of Pinus taeda and Pinus rigida contained 12.00%, 13.19% crude protein respectively and sixteen protein-constituting amino acids and three unknowns were detected on the chromatogram. 2. Main components of the protein-constituting amino acids were leucines, valine, alanine, glutamic acid and aspartic acid. 3. The free amino acids such as leucines, valine, tyrosine, alanine and glutamic acid increased evidently during germination of the pine pollen.

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표고버섯의 아미노산(酸) 및 비타민분석(分析) (Analysis of Amino Acid and Vitamin in Oak Mushroom (Lentinus edodes Sing))

  • 황병호
    • Journal of the Korean Wood Science and Technology
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    • 제11권1호
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    • pp.18-24
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    • 1983
  • 추용(椎茸)버섯의 영양학적(營養學的) 기초자료(基礎資料)의 보강(補强) 및 미분석자료(未分析資料)를 제공(提供)하기 위하여 일반분석(一般分析), amino 산(酸) 분석(分析), vitamin 분석(分析)을 행한 결과(結果)를 요약(要約)하면 다음과 같다. 1) 일반분석(一般分析)은 이미 발표(發表)된 영양분석표(榮養分析表)의 기본(基本) 분석치(分析値)와 유사(類似)하였으며, 2) Amino 산(酸)은 모두 17종 즉, aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, lecucine, tyrosine, phenylalanine, tryptophan, lysine, bistidine, arginine 등이 검색(檢索)되었는데, 가장 많이 함유(含有)된 성분(成分)은 glutamic 산(酸)이었다. 3) 필수(必須) 아미노산 8종(種) 즉, threonine, valine, methionine, isoleucine, lecucine, phenylalanine, tryptophan, lysine 등이 모두 함유(含有)되어 있어서 매우 중요(重要)한 식품(食品)이라는 것을 알수 있었다. 4) Vitamin은 총(總) 7종(種) 즉, A(${\beta}$-carotene), $B_1$ (thiamin), $B_2$(riboflavin), C (ascorbic acid), E (${\alpha}$-tocopherol), Niacin, Cholinechloride이 분석(分析)되었으며 가장 많은 양(量)은 Cholinechloride 이었다.

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소나무 잔나비버섯의 아미노산, 비타민 및 무기물 분석 (Analysis of Amino Acids, Vitamins and Minerals of Fruiting Body of Fomitopsis pinicola)

  • 정길록;신현재;차월석
    • 생명과학회지
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    • 제16권7호
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    • pp.1123-1126
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    • 2006
  • 소나무 잔나비버섯은 약용으로 이용하고자 구성아미노산, 유리아미노산, 비타민, 무기물의 성을 분석 검토한 결과 건조버섯 100 g당 총아미노산은 2901.5 mg, 유리아미노산 68.67 mg, 비타민 276.91 mg, 무기물은 347.37 mg이었다. 총 아미노산의 구성아미노산은 18종의 아미노산으로 구성되었으며, glutamic acid가 457 mg로 가장 많이 함유되었고, threonine, glycine, aspartic acid, valine등의 순으로 각각 279 mg, 241 mg, 233 mg, 233 mg의 함량이었다. 유리아미노산 중에서는 glutamine이 17.61 mg으로 가장 많이 함유되어 있고, arginine, tryptophan, threonine, glutamic acid, isoleucine, phenylalanine, tyrosine, valine이 각각 6.51 mg, 5.65 mg, 4.38 mg, 4.02 mg, 3.89 mg, 2.69 mg, 1.51 mg, 1.87 mg등의 순으로 구성되었다. 소나무잔나비 버섯의 무기물 구성 중, 성분 P가 77.57 mg로 가장 많이 함유되었고 다음으로 Mg, Fe, Ca, Na, Zn,등의 순이었다. 풍부한 아미노산 및 무기물 함량과 더불어 소나무잔나비 버섯의 비타민 성분 중, vitamin E는 275.76 mg으로 노화방지에 효과가 높을 것으로 사료되고, 추후 기능성 식품으로 이용적 가치가 높을 것으로 판단된다.