• 제목/요약/키워드: Valine

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Purification and Chemical Characterization of Antibiotic MT-497 Produced by Streptomyces nigrifaciens GMT-497 (Streptomyces nigrifaciens GMT-4967이 생산하는 항생물질 MT-497의 분리와 화학적 특성)

  • 이영선;안순철;윤병대;민태익;이정형;이현선;안종석;민태익
    • Microbiology and Biotechnology Letters
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    • v.19 no.6
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    • pp.604-609
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    • 1991
  • Antibiotic MT-497 was purified from the culture broth of Streptomyces nigr$^1H-NMR$ and composition of amino acids, MT-497 was identified as one of the actinornycin antibiotics containing actinocin chromophore and the peptide with threonine, proline, methyl valine, sarcosine and aspartic acid.

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Studies on Amino Acids in the Frog Liver (Bombina orientalis Boulenger) (무당개구리 간의 아미노산 성분에 관한 연구)

  • 박상윤;오석훈
    • The Korean Journal of Zoology
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    • v.3 no.2
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    • pp.5-8
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    • 1960
  • Paper partition chromatography has been applied to estimate the amino acids in the liver of Bombina orientalis Boulenger. The results obtained are as follows : 1) The presence of cystine, serine, glycine, threonine, alanine, $\beta$-alanine, tyrosine, histidine, asparagine, tryptophane, methionine, valine, phenylalanine, leucine and an unknown substance has been demonstrated in the alcoholic extracts of the material. 2) The presence of glutamine, cystine, serine , glycine, arginine, threonine, histidine, alanine, methionine, proline, valine ,phenylalanine, leucine, and two unknown substances has been demonstrated in the acid-hydrolyzed liver. 3) Tyrosine, asparagine, and trypotphane have been demonstrated only in the alcoholic extract. 4) Proline, glutamine and arginine have been demonstrated only in the acid-jydrolyzed liver.

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p-Coumaroylamino Acids from Yeast-Elicited Ephedra distachya Cultures

  • Song, Kyung-Sik;Sankawa, Ushio;Ebizuka, Yutaka
    • Archives of Pharmacal Research
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    • v.17 no.1
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    • pp.48-50
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    • 1994
  • Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.

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Effects of Ginseng-cake on Growth and Biochemical Components of Rats (인삼박이 흰쥐의 성장과 체성분에 미치는 영향)

  • 이정실;김을상;김해중
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.4
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    • pp.329-336
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    • 1991
  • This study was carried out to investigate the effect of ginseng-cake on growth and biochemical components of rats. The content of crude protein in ginseng-cake was 17.2% as dry basis. Methionine, isoleuine and valine were limiting amino acids in order and arginine was 23.6% in total amino acids. Body weight gain, food efficiency ratio, organ weight and biochemical components in plasma were significantly lower in ginseng-cake based diet group than in control diet group and those of ginseng-cake diet group supplemented with methionine, isoleucine and valine were significantly increased than those of ginseng-cake based diet group, but 소id not reach to those of control diet group.

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The Taste Conpounds of the Fermented Cod-roe, Gadus Macrocephlus - Changes of Free Amino Acids during the Fermentation of Cod-roe - (대구알젓의 맛성분 제I보 : 대구알젓의 유리아미노산)

  • 박재옥
    • Journal of the Korean Home Economics Association
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    • v.20 no.4
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    • pp.99-105
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    • 1982
  • Changes of free amino acids as taste compunds during the fermentation of cod-roe, Gadus marcrocephlus, were analyzed by a amino acid autoanalyzer. Leucine, lysine, valine, isoleucine and arginine were dominent amino acids in fresh cod-roe extracts, and the amounts of leucine (797.3mg%, on moisture and salt free base), lysine (577.7mg%) and valine(487.4mg%0 were 54.9% of total free amino acids. The contents of methionine, histidine, tyrosine and phenylanine were lower, and aspartic acid, glycine, threonine, glutamic acid and serine were detected trace amount. The free amino acids analyzed in this experiment were not changed in composition but changed in the amounts during 80 days of the fermentation. Total free amino acids were increased until 59 days of fermentation and than decreased gradually.

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A New Technique for Single Spore Isolation of Two Predacious Fungi Forming Constricting Ring

  • Singh, Kalika Prasad;Kumar, Dharmendra;Bandyopadhyay, Pinaki
    • Mycobiology
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    • v.32 no.4
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    • pp.197-198
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    • 2004
  • A new technique for single spore isolation was developed for predacious fungi forming constricting rings directly on the spores using Dactylaria brochopaga and Arthrobotrys dactyloides. Constricting rings were induced directly on the spores by transferring the spores in 25 ppm solution of DL-Valine in sterile distilled water. Freshly hatched and thoroughly washed second stage juveniles of Meloidogyne incognita were transferred into cavity blocks containing induced rings for trapping and killing of nematodes. The killed nematodes were surface sterilized with streptomycin and inoculated into petri dishes containing maize meal agar media with 100 ppm streptomycin. The petri dishes were incubated at $29{\pm}1^{\circ}C$ for few days which yielded axenic culture of these fungi.

On the Amino Acid Composition in the Pollen of Pines and Changes of the Composition during Germination of Pollen (송류화분(松類花粉)의 아미노산(酸) 조성(組成) 및 화분발아(花粉發芽)에 따른 그 조성(組成)의 변화(變化))

  • Hong, Sung Ok
    • Journal of Korean Society of Forest Science
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    • v.6 no.1
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    • pp.43-48
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    • 1967
  • In the present experiment the amino acid composition in the pollen of Pinus taeda and Pinus rigida and methbolic changes of the composition occurring in germinating pollen were examined by the method of paper chromatography. The results obtained in this study are as follows. 1. The mature pollen of Pinus taeda and Pinus rigida contained 12.00%, 13.19% crude protein respectively and sixteen protein-constituting amino acids and three unknowns were detected on the chromatogram. 2. Main components of the protein-constituting amino acids were leucines, valine, alanine, glutamic acid and aspartic acid. 3. The free amino acids such as leucines, valine, tyrosine, alanine and glutamic acid increased evidently during germination of the pine pollen.

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Analysis of Amino Acid and Vitamin in Oak Mushroom (Lentinus edodes Sing) (표고버섯의 아미노산(酸) 및 비타민분석(分析))

  • Hwang, Byung-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.11 no.1
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    • pp.18-24
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    • 1983
  • Oak mushroom (Lentinus edodes Sing.) has been used as one of vegetable foods for a long time, because it has particular odour, taste and abundant nutritive material. Its cultivation has been done extensively, but its checmical analysis of the components is curiously limited. It is the purpose of this study to analyse its component chemically and to get basic data in terms of nutrition science. The results are summarized as follows: 1. It was analysed that the total number of amino acid is 17 different kinds, mainly glutamic acid. 2. All the essential amino acids which are lecucine, lysine, threonine, tryptophan, valine, phenylalanine, isoleucine, tyrosine, and methionine were found in this chemical analysis. 3. The seven kinds of vitamins are analysed, which are A(${\beta}$-carotene), $B_1$ (thiamin), $B_2$(riboflavin), C(ascorbic acid), E(${\alpha}$-tocopherol), Niacin, choline chloride.

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Analysis of Amino Acids, Vitamins and Minerals of Fruiting Body of Fomitopsis pinicola (소나무 잔나비버섯의 아미노산, 비타민 및 무기물 분석)

  • Ding, J.L.;Shin, H.J.;Cha, W.S.
    • Journal of Life Science
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    • v.16 no.7 s.80
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    • pp.1123-1126
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    • 2006
  • The nutritional composition of Fomitopsis pinicola (F. pinicola) fruiting body has been analyzed for medicinal and edible uses. The contents of crude fibers, carbohydrates, crude protein, moisture, crude fats and ashes were 43.3%, 26.3%, 12.8%, 12.6%, 3.3% and 1.7%, respectively. Eighteen amino acids were found in F. pinicola. Among total amino acids, glutamate content was the highest (457 mg/100 g dry mushroom) and arginine, glycine, valine, aspartate and isoleucine were followed. Concerning free amino acids, glutamine, arginine, trytophan, and glutamate were dominant. The vitamin E content was the highest (276 mg/100 g dry mushroom), then vitamin H and vitamin B, were followed. The mineral contents were as follows: K 165.06 mg, P77.57 mg, Mg 46.11 mg, Fe 21.56 mg, and Ca 16.90 mg based on 100 g dry mushroom.