• Title/Summary/Keyword: VP1-2A

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Dose and Image Assessment according to Radiologic Factors Variation at Digital Humerus X-ray Examination (디지털 환경에서 Humerus 검사 시 촬영인자 변화에 따른 선량 및 화질 평가)

  • Kim, Seong Min;Hong, Seon Sook;Lee, Kwan Sup;Ha, Dong Yun
    • Korean Journal of Digital Imaging in Medicine
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    • v.14 no.2
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    • pp.1-8
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    • 2012
  • Purpose : We aim at presenting the optimum radiologic factor through the evaluation of dose variation and of image quality through the use of a grid in Humerus examination and the change of dose because of the change of radiologic factor. Materials and Methods : We divided it in 3 cases: when using a grid or not and when using IP(Image Plate) in a digital system. Also, as fixing kVp to 70kVp it changed mAs, and fixing mAs to 10 it changed kVp, we put up resolution chart and Burger rose phantom on the acrylic phantom of 7cm (the same level of Humerus) to evaluate the dose and image. We used Image J program to evaluate the quantitative resolution of the obtained image, and made the qualitative evaluation and statistical analysis of the image saved in PACS for 20 radiologic technologist with more than 10 years of experience in order of evaluate its contrast. We used SPSS10(SPSS Inc. Chicago, Illinois) for statistical analysis. Results : We observed the analytic result of resolution by the change of kVp that it was $4.539dGycm^2$ in 60kVp and $757.472dGycm^2$ in 75kVp, which increased about 64.6% of dose, while for the resolution it had the pixel value 30.7% better with 851 in 60kVp than 651 in 75kVp. Also, we analyzed the result of resolution by the change of mAs that it was $3.106dGycm^2$ in 5mAs, and $12.470dGycm^2$ in 20mAs, which increased about 400% of dose, while for the resolution DR had 678 in 5mAs, and 724 in 20mAs that increased about 6.8% of resolution. We made the qualitative evaluation of contrast by the change of kVp that DR showed the higher quality than CR, but the contrast by the change of kVp had no special different at the moment of visual evaluation, nor statistically significant difference(P>0.05). We observed the qualitative evaluation of contraste by the change of mAs that the contrast increased as DR increased mAs, and had statistically significant difference(P<0.05). On the other hand, CR had no significant difference for more than 10mAs nor statistically significant difference(P>0.05). Conclusion : In case of some patients with radiographic exposure by the repeated examination such as emergent patient or Follow up patient, they are considered to try to limit the use of a grid, to set kVp under 65kVp in fixed mode, to select less than 10mAs and to reduce the possibility of patient being bombed.

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Effects of HIF-1α/VP16 Hybrid Transcription Factor on Estrogen Receptor in MCF-7 Human Breast Cancer Cells

  • Cho, Jung-Yoon;Park, Mi-Kyung;Lee, Young-Joo
    • Biomolecules & Therapeutics
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    • v.13 no.4
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    • pp.227-231
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    • 2005
  • The estrogen receptor (ER) is activated and degraded by estrogen. We have examined ER downregulation and activation under hypoxia mimetic conditions. Cobalt chloride induced ER downregulation at 24 h of treatment. This degradation involved hypoxia-inducible factor-1$\alpha$ (HIF-1$\alpha$) as examined by using a constitutively active form of HIF-1$\alpha$, HIF-1$\alpha$/VP16, constructed by replacing the transactivation domain of HIF-1$\alpha$ with that of VP16. Western blot analysis revealed that E2-induced ER downregulation was observed within ${\~}6h$, whereas HIF-1$\alpha$/VP16-induced ER degradation was observed within 12${\~}$20h. HIF-1$\alpha$/VP16 activated the transcription of estrogen-responsive reporter gene in the absence of estrogen. These results suggest that ER downregulation and activation under hypoxia maybe mediated in part by a HIP-1$\alpha$ expression.

Effects of Exposure Dose Reduction by Optimization of Automatic Exposure Control Factors in Digital Radiographic Examination of Paranasal Sinus (부비동 디지털 엑스선검사에서 자동노출제어 조절인자의 최적화를 통한 조사선량 감소 효과)

  • Jeong, Min-Gyu;Seoung, Youl-Hun
    • Journal of radiological science and technology
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    • v.44 no.3
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    • pp.173-181
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    • 2021
  • The purpose of this study was to reduce dose while maintaining image quality during digital radiographic examination of paranasal sinus by using the automatic exposure control (AEC) system. The tube voltage was set as six stages that increased by about 10 kVp to 70 kVp, 81 kVp, 90 kVp, 102 kVp, 109 kVp and 117 kVp. And then the AEC system conditions were consisted of 9 setting environments, that change mode of the sensitivity (S200, S400, S800) and the density (+2.5, 0, -2.5). We measured automatically exposed tube current (mAs) under 54 conditions with combined these, and assessed SNR and PSNR through the acquired images. In addition, four radiologists performed a qualitative assessment of the acquired images for each combination on a five-point scale of the Likert. As a result, the lowest dose and the highest values of SNR and PSNR in images with a qualitative assessment more than 4 point were the AEC control factors of 90 kVp, S800, D2.5. We applied this condition to the clinical trial, it showed an effect of 83.1% reduction in exposure radiation dose (mR). Therefore, AEC system could be used as dose reduction technology if it understood and used related regulatory factors and physical characteristics.

Effects of Vacuum Precooling on Shelf Life of Pleurotus eryngii during PE Packaging Storage (큰느타리 버섯의 PE 포장 저장 중 선도에 미치는 예냉처리 효과)

  • Beik, Kyung-Yean;Lee, Ye-Kyung;Kim, Jae-Won;Park, In-Sik;Kim, Soon-Dong
    • Food Science and Preservation
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    • v.16 no.2
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    • pp.166-171
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    • 2009
  • The effects of vacuum precooling(VP) on the shelf-life of polyethylene film(PE) packaged King oyster mushrooms(Pleurotus eryngii) during storage at $-1^{\circ}C$ were investigated. VP was conducted below $0^{\circ}C$ in a $-1^{\circ}C$ cold chamber of 40 minutes, and mushrooms were stored for 30 days in batches of 1kg. The weight loss of the VP-treated mushroom was slightly lower than that of control. The $O_2$ concentrations of VP-treated mushroos, within 4 days of storage, were 2.44-14.50 %/kg-package/hr, thus higher than control values(2.01-8.19%/kg-package/hr). $CO_2$ generation of VP-treated mushrooms, again within 4 days of storage, was 0.47%/kg-package/hr, thus lower than that of controls(0.58%/kg-package/hr). The $CO_2/O_2$ ratio peaked on day 4 of storage in the control group, tbut no such peak was observed in VP-treated mushrooms. In the VP-treated fungi, lightness was higher, and redness and yellowness lower, than in controls, at all storage times.. In VP-treated mushrooms, strength, hardness and chewiness were significantly higher than in controls, but there were no significant differemces in springiness or cohesiveness. Softening and breakdown of under-cap wrinkles were observed in control mushrooms stored for 30 days, but occurred to a lesser extent in VP-treated fungi. Stipe reticulum tissue vessels in the 30 day-stored VP-treated mushrooms were relatively well-defined and clear, but were softer and diffuse in the control fungi. The results thus confirmed that VP after harvest enhanced mushroom shelf-life and PE packaging prolonged storage time. The data will have industrial applications.

Introduction of VP6 Gene into Potato Plant by Agrobacterium-mediated Transformation and Analysis of VP6 Expression in Transgenic Potatoes (Rotavirus VP6 유전자의 감자식물체내로의 도입과 형질전환체의 발현분석)

  • Youm, Jung-Won;Jeon, Jae-Heung;Jung, Jae-Yeol;Lee, Byoung-Chan;Kang, Won-Jin;Kim, Mi-Sun;Kim, Chul-Joong;Joung, Hyouk;Kim, Hyun-Soon
    • Journal of Plant Biotechnology
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    • v.29 no.2
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    • pp.93-98
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    • 2002
  • A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.

Evaluation of Image According to Exposure Conditions using Contrast-Detail Phantom for Chest Digital Radiography (흉부 디지털 방사선 촬영 시 C-D phantom을 이용한 촬영조건에 따른 영상 평가)

  • Lee, In-Ja;Kim, You-Hyun;Kim, Chang-Nam;Lee, Chang-Yeob;Park, Kye-Yeon
    • Journal of radiological science and technology
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    • v.32 no.1
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    • pp.25-32
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    • 2009
  • To find out proper photographing conditions in the chest DR imaging, the evaluation of images using the C-D phantom was carried out on relationship of identification capability, graininess, and exposure ratio. The conclusions were obtained as follows. 1. The patient's entrance skin Exposure (ESE) was decreased as tube voltage was increased. 2. According to the tube voltage change, the C-D phantom's identification capability of the exposure conditions was most visible at 110 kVp. 3. The identification capability according to the exposure ratio (mAs) change was most visible at 90 kVp for 0.5 times of low exposure ratio and at 110 kVp for 1.5 times. Therefore, it is known that the images were able to be better identified at a high exposure than a low exposure. 4. The graininess according to the exposure ratio at tube voltage of 110 kVp resulted in the best thing at 1.5 times of ratio when the exposure ratio was 1.5 times increased and the tube voltage was changed, the graininess showed the best result at 110 kVp. Therefore, the patient's exposure dose was low when kVp was increased and the adequate kVp was found to be 110. The image was better identified when exposure ratio was 1.5 times compared to 1.0 times. The graininess was also good when the exposure ratio became 1.5 times. The tube voltage was good at 110 kVp. However, once the exposure ratio is increased, the amount of radiation dose that the patients received get increased, so that the exposure condition has to be thoroughly considered.

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A Study on Exposure Technics in Roentgenography of the Stomach (위조영촬영시(胃造影撮影時) 촬영조건(撮影條件)에 관(關)한 연구(硏究))

  • Kyong, Kwang-Hyon;Kim, Heung-Tai;Kwon, Dal-Kwan;Sun, Han-Kyung;Huh, Joon
    • Journal of radiological science and technology
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    • v.4 no.1
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    • pp.45-53
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    • 1981
  • In examinations of the stomach roentgenography, it is imperative to obtain adequate film density throughout all its different regions. Therefore, it is necessary to use more sophiscated exposure techniques. In order to achieve these purpose, the radiologic technologists must be measured abdominal thickness in variations with patient positions. In consideration of these problem, the author was made an experiment on correction method of kVp and mAs by abdominal thickness in roentgenography of the stomach. The results were summarized as follws: 1. When the patient in erect position, abdominal thickness was the most thickened at the level of 3cm inferior to umbilicus without regard to body habitus and it was the most thickened at the level of 3cm superior to umbilicus in prone and supine position. 2. As a result of measuring film density for stomach, the adequate film density was represented from 0.70 to 2.49 in erect position and $0.28{\sim}1.18$ in supine position, $0.5{\sim}2.45$ in prone position. 3. In order to obtain uniform film density in 1.25, the correction factor for kVp by abdominal thickness was represented average ${\pm}4.5kVp\;per\;{\pm}1cm$ in a fixed 50 mAs, and average ${\pm}3.9kVp\;per\;{\pm}1cm$ in a fixed 100mAs. 4. In order to obtain uniform film density in 1.25, the correction factor for mAs by abdominal thickness was represented average ${\pm}30.9%\;per\;{\pm}1cm$ in a fixed 80 kvp and ${\pm}26.9%\;per\;{\pm}1cm$ in a fixed 100kVp.

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Quality Changes of Pork in Relation to Packaging Conditions During Chilled Storage in Households

  • Lee, Keun Taik;Jang, Min Jun
    • Food Science of Animal Resources
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    • v.33 no.4
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    • pp.448-455
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    • 2013
  • Proper storage of left-over meat in a household refrigerator is important for extending its shelf-life and assuring its safety until it is next used. Various fresh meat packaging methods were examined to determine their effects on the quality characteristics of pork loins during household storage at $5^{\circ}C$. The packaging methods include 1) wrapping in a polyethylene pouch (WP), 2) keeping in an air-tight plastic container (CP), and 3) using a household vacuum packaging machine (VP). The fastest increase in total aerobic bacteria during storage occurred in the WP samples, followed by the CP and VP samples. The count of Pseudomonas spp. was found to be lowest in the VP, and then the CP and WP samples. Enterobacteriaceae grew fastest in the WP samples, followed by the CP and VP samples. The WP samples also incurred the most significant increase in thiobarbituric acids and volatile basic nitrogen values over the storage period, as compared to the CP and VP samples. Off-odour at 30 min after opening the package, was first notable at day 11 in WP samples, but only at day 15 in the CP and VP samples. The colour also deteriorated earlier in the WP samples than in the CP and VP samples.

A Study on Optimum kVp in Four Spot Films of Stomach Examinations (위(胃) 사등분할촬영시(四等分割撮影時) 적정(適正)한 관전압(管電壓)에 관(關)한 검토(檢討))

  • Kim, Wha-Gon;Kyong, Kwang-Hyon;Kim, Young-Hwan;Huh, Joon
    • Journal of radiological science and technology
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    • v.3 no.1
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    • pp.37-41
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    • 1980
  • In an attempt to provide optimum kVp for four spot films of stomach examinations, we measured experimentally film density and scatter radiation with field size. And to investigate the effect of concentrations in barium sulphite and kVp in spot films of stomach fluoroscopy were carried out and the following results were obained. 1. The entire density of film by field size has the sharpest increase from $10cm^2$ to $100cm^2$, and relatively flattened curve beyond $500cm^2$ in field size. 2. The quantity of scatter radiation reaching an X-ray film depends upon field size: the larger the fields, the more scatter radiation. 3. It is necessary for increasing 3 to 5kVp as for the absence of barium sulphite and 5 to 7 kVp in the case of 20 per cent and 25 per cent in barium sulphite concentrations to produce uniform density in the four spot films for stomach fluoroscopy.

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A COMPARATIVE STUDY ON RADIOPACITY OF CANAL FILLING AND RETROGRADE ROOT-END FILLING MATERIALS (근관 충전재 및 치근단 역충전재의 방사선 불투과성에 관한 비교 연구)

  • Kim, Yong-Sang;Kim, Seo-Kyong;Hwang, Yun-Chan;Hwang, In-Nam;Oh, Won-Mann
    • Restorative Dentistry and Endodontics
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    • v.33 no.2
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    • pp.107-114
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    • 2008
  • This study was performed to assess the radiopacity of a variety of canal filling and retrograde root-end filling materials according to the specification concerning root canal obturation materials. Ten materials including Gutta-percha pellets, amalgam, Fuji II LC, $Dyract^{(R)}$ AP, Super $EBA^{(R)}$, $IRM^{(R)}$, AH $26^{(R)}$, $Sealapex^{TM}$, Tubli-$Seal^{TM}$, and dentin were evaluated in this study. In the first part, densitometric reading of an each step of aluminum step wedge on occlusal film were performed at 60 kVp (0.2, 0.3, 0.4 s), 70 kVp (0.2, 0.3, 0.33 s) to decide appropriate voltage and exposure time. In the second part, ten specimens which are 5 mm in diameter and 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 mm in thickness, were fabricated from each material studied. The specimens were radiographed simultaneously with an aluminum step wedge under decided condition (60 kVp, 0.2 s). The mean radiographic density values of the materials were transformed into radiopacity expressed equivalent thickness of aluminum (mm Al). The following results were obtained. 1. Among the various conditions including 0.2 s, 0.3 s, 0.4 s at 60 kVp and 0.2 s, 0.3 s, 0.33 s at 70 kVp, the appropriate voltage and exposure time that meet the requirement of density from 0.5 to 2.0 was 0.2 s at 60 kVp. 2. All of the materials in this study had greater radiopacity than the minimun level recommended by ISO No. 4049 standards. 3. Most of the materials had greater radiopacity than 3 mm Al requirement of ANSI/ADA specification No. 57 (2000) and ISO No. 6876 (2001) standards except for Fuji II LC and Dyract. It suggests that all experimental canal filling and retrograde root-end filling materials have a sufficient radiopacity that meet the requirement concerning root canal obturation materials except for Fuji II LC and Dyract.