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http://dx.doi.org/10.5010/JPB.2002.29.2.093

Introduction of VP6 Gene into Potato Plant by Agrobacterium-mediated Transformation and Analysis of VP6 Expression in Transgenic Potatoes  

Youm, Jung-Won (Plant Cell Biotechnology Lab., KRIBB)
Jeon, Jae-Heung (Plant Cell Biotechnology Lab., KRIBB)
Jung, Jae-Yeol (Plant Cell Biotechnology Lab., KRIBB)
Lee, Byoung-Chan (Plant Cell Biotechnology Lab., KRIBB)
Kang, Won-Jin (Plant Cell Biotechnology Lab., KRIBB)
Kim, Mi-Sun (Plant Cell Biotechnology Lab., KRIBB)
Kim, Chul-Joong (Lab. of Infectious Disease. Chungnam National University)
Joung, Hyouk (Plant Cell Biotechnology Lab., KRIBB)
Kim, Hyun-Soon (Plant Cell Biotechnology Lab., KRIBB)
Publication Information
Journal of Plant Biotechnology / v.29, no.2, 2002 , pp. 93-98 More about this Journal
Abstract
A VP6 fragments was subcloned with BamHI in the binary pMBP-1 vector under Califlower Mosaic Virus (CaMV) 355 promoter and neomycin phosphotransferase II (npt II) gene. The recombinant binary vector was mobilized into Agrobacterium-tumefaciens LBA4404 by the freeze-thaw method and potato (Solanum tubensum L. cv Desiree) was transformed by modified leaf-disc cocultivation. Shoots were induced on MS medium with 0.01 mg/L NAA, 0.1 mg/L GA$_3$, 2.0 mg/L Zeatin, 100.0 mg/L kanamycin, 500.0 mg/L carbenicillin. In order to identify the copy number of VP6 into potato plant, total genomic DNA was isolated from transgenic potato and analysed by Southern blotting. Genomic DNA and total mRNA analysis demonstrated the incorporation of the foreign gene into the potato genome, as well as their transcription.
Keywords
Solanum tuberosum L. cv Desiree; VP6 gene; CaMV 355 promoter; NPT II;
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