• Journal of Nutrition and Health
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• 제21권4호
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• pp.260-267
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• 1988

Forteen healthy women, with normal serum cholesterol levels, were supplemented with two egg yolks daily for the 1st week and four egg yolks for the 2nd week to their customary diets for 2 weeks period. Serum cholesterol, triglyceride, phospholipid and lipoprotein fractions were determined. Serum cholesterol and phospholipid levels were not affected by the supplementation of egg yolks. However, triglyceride level was significantly increased by the supplementation of four egg yolks at the end of the 1st week, but the level remained normal range. The serum lipoprotein pattern was influenced by the supplementation of egg yolks : HDL fraction significantly decreased, while LDL and VLDL fractions significantly increased.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.392.3-393
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• 2002

The increase of triglyceride in blood can be a signal of an increasing danger of arterial diseases when insulin resistance. diabetes. HDL -cholesterol decrease is accompanied. It is adjusted to triglyceride level in blood by a balance. which seems to be absorbed from VLDL metabolism in liver and by lipoprotein lipase activity. The hyper-triglyceride disease treatment proposal role should match with suppression does into liver or elimination of a triglyceride. (omitted)

• 한국식품영양학회지
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• 제11권1호
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• pp.41-46
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• 1998

청둥오리 압란유의 기능적 특성을 알아보기 위하여 흰쥐를 모델로 하여 streptozotocin(STZ)을 투여하여 당뇨병을 유발시킨 다음, 혈액 중의 당 농도, 지질의 변화 및 동맥경화 지표와의 상관관계를 검토하였고, 또한 Sarcoma-180을 쥐에 이식하여 이에 대한 항암 효과를 실험하였다. STZ로 당뇨병을 유발시킨 흰쥐는 청둥오리의 압란유를 15일간 투여하였던바 혈중의 당 농도는 정상상태로 유지되었고 phospholipid 및 triglyceride의 함량은 STZ를 처리한 압란유의 경우에는 증가하였으나, STZ 처리군에 압란유를 투여하였을 때 감소하였다. 혈중 total cholesterol, LDL + VLDL의 cholesterol 및 동맥경화의 지표는 STZ 처리군에서는 증가하였으나, 시료를 150mg/kg 투여시에는 감소하였다. 한편 Sarcoma-180에 대한 성장억제율은 압란유를 150mg/kg 투여시 63.89%로 나타났고 수명연장 실험에서는 압란유의 경우 15.4%로 나타났다.

• BMB Reports
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• 제33권2호
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• pp.148-154
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• 2000

The glycation process plays an important role in accelerated atherosclerosis in diabetes, and the uptake of atherogenic lipoproteins by macrophage in the intima of the vessel wall leads to foam cell formation, an early sign of atherosclerosis. Besides the lipolytic action on the plasma triglyceride component, lipoprotein lipase (LPL) has been reported to enhance the cholesterol uptake by arterial wall cells. In this study, some properties of LPL-mediated low-density lipoprotein (LDL) uptake and the effect of LDL glycation were investigated in RAW 264.7 cell, a murine macrophage cell line. In the presence of LPL, $^{125}I$-LDL binding to RAW 264.7 cells was increased in a dose-dependent manner. At concentrations greater than $20\;{\mu}g/ml$ of LPL, LPL-mediated LDL binding was increased about 17-fold, achieving saturation. Without LPL, both very low-density lipoprotein (VLDL) and high-density lipoprotein (HDL) were ineffective in blocking the binding of $^{125}I$-LDL to Cells. However, LPL-enhanced LDL binding was inhibited about 50% by the presence of VLDL, while no significant effect was observed with HDL. Heat inactivation of LPL caused a 30% decrease of LDL binding. In the presence of LPL, the cells took up 40% of cell-bound native LDL. No significant difference was observed in cell binding between native and glycated LDL. However, the uptake of glycated LDL was significantly greater than that of native LDL, reaching to 70% of the total cell bound glycated LDL. These results indicate that LPL can cause the significant enhancement of LDL uptake by RAW 264.7 cells and the enhanced uptake of glycated LDL in the presence of LPL might play an important role in the accelerated atherogenesis in diabetic patients.

• Journal of Nutrition and Health
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• 제27권7호
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• pp.687-698
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• 1994

The effects of age and dietary fatty acid composition on lipid metabolism were investigated in Sprague-Dawley strain male rats. These animals weighing 88.6$\pm$2.2g were fed 10% dietary fat(W/W, 20% of total energy) with 0.5, 1, 2 P/S ratio and in each P/S ratio there were three different levels of n-6/n-3 fatty acid ratio ; 2, 4, 8. The experimental period was 1 month, 6 months and 12 months. The results of this study were as follows. The body weight of rats increased rapidly for the first two months, then increased slowly until 7 to 8 months. After 10 months of dietary regimen their weight decreased. The weight of liver, kidney and epidydimal fat pad increased along with the body weight and then decreased in the 12 months. Plasma total lipid increased with age and it decreased significantly when P/S ratio of dietary fatty acid was high. In creased with age and it decreased significantly when P/S ratio of dietary fatty acid was high. In creasing n-3 fatty acid intake in each P/S ratio resulted in lower plasma total lipid although was not statistically significant. The amount of plasma total cholesterol increased at 6 months, but decreased at 12 months. In case of 1, 12 months, increasing P/S ratio significantly plasma total cholesterol and LDL-cholesterol were decreased and hepatic cholesterol was increased, VLDL-HDL-cholesterol did not changed. The n-6/n-3 ratio did not affect any of theses. The amount of plasma triglyceride and VLDL-triglyceride increased at 6 month then decreased. When the rats consumed higher amount of n-3 fatty acid in each P/S ratio, their plasma triglyceride and hepatic triglyceride increased at 1, 12months.

• 한국응용과학기술학회지
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• 제3권2호
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• pp.35-40
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• 1986

자동산화유의 독성을 구명하기 위하여 autoxidized methyl linoleate(AOML)의 일정량을 mouse에 1 일 1 회 20 일간 경구 투여하여 혈청 및 간의 지질대사에 미치는 영향을 검토한 결과는 다음과 같다. 실험군의 혈청 TBA값과 혈청 triglyceride, phospholipid 및 total cholesterol양은 정상군에 비해 모두 증가되었으며, 전기영동 결과 혈청 lipoprotein의 조성 백분율은 albumin이 약 21% 증가된 반면, VLDL은 약 70% 그리고 LDL은 약 33% 감소되어 있었다. 실험군의 간에서의 POV, COV 및 TBA값은 모두 정상군에 비해 크게 증가하였으며, 또한 간의 triglyceride, phospholipid 및 total cholesteroldml 양도 증가하는 경향을 나타내고 있었다. 한편, 실험군의 간에서의 형태학적 변화로는 정상군에 비해 간세포의 손상과 지질의 축적이 관찰 되었다.

• Nutrition Research and Practice
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• 제10권5호
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• pp.477-486
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• 2016

BACKGROUND/OBJECTIVES: Consumption of pine nut oil (PNO) was shown to reduce weight gain and attenuate hepatic steatosis in mice fed a high-fat diet (HFD). The aim of this study was to examine the effects of PNO on both intestinal and hepatic lipid metabolism in mice fed control or HFD. MATERIALS/METHODS: Five-week-old C57BL/6 mice were fed control diets containing 10% energy fat from either Soybean Oil (SBO) or PNO, or HFD containing 15% energy fat from lard and 30% energy fat from SBO or PNO for 12 weeks. Expression of genes related to intestinal fatty acid (FA) uptake and channeling (Cd36, Fatp4, Acsl5, Acbp), intestinal chylomicron synthesis (Mtp, ApoB48, ApoA4), hepatic lipid uptake and channeling (Lrp1, Fatp5, Acsl1, Acbp), hepatic triacylglycerol (TAG) lipolysis and FA oxidation (Atgl, Cpt1a, Acadl, Ehhadh, Acaa1), as well as very low-density lipoprotein (VLDL) assembly (ApoB100) were determined by real-time PCR. RESULTS: In intestine, significantly lower Cd36 mRNA expression (P<0.05) and a tendency of lower ApoA4 mRNA levels (P = 0.07) was observed in PNO-fed mice, indicating that PNO consumption may decrease intestinal FA uptake and chylomicron assembly. PNO consumption tended to result in higher hepatic mRNA levels of Atgl (P = 0.08) and Cpt1a (P = 0.05). Significantly higher hepatic mRNA levels of Acadl and ApoB100 were detected in mice fed PNO diet (P<0.05). These results suggest that PNO could increase hepatic TAG metabolism; mitochondrial fatty acid oxidation and VLDL assembly. CONCLUSIONS: PNO replacement in the diet might function in prevention of excessive lipid uptake by intestine and improve hepatic lipid metabolism in both control diet and HFD fed mice.

• Asian-Australasian Journal of Animal Sciences
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• 제23권9호
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• pp.1221-1228
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• 2010

This study aimed to examine the effect of overfeeding on biochemical parameters and lipoprotein-lipase (LPL) mRNA expression in different tissues associated with hepatic lipogenesis in Sichuan white and Landes geese. Fifty healthy male Sichuan white geese and fifty male Landes geese (Cygnus atratus) were hatched on the same day under the same feeding conditions and were selected as experimental animals. After overfeeding for 14 days (from 14 weeks to 16 weeks) and then slaughtering, the biochemical changes of hepatic lipogenesis were evaluated. Results showed that i) in Landes geese, the plasma concentration of glucose was higher (p<0.001), while plasma concentrations of insulin and VLDL were both lower (p<0.01); ii) the LPL mRNA level in pectoralis muscle and leg muscle of the overfed groups in both breeds was higher (p<0.05) than in the control groups; iii) in Sichuan white geese, the proportion of fatty liver weight was positively correlated with plasma triacylglycerols (TG)(p<0.05) and VLDL concentrations (p<0.05), while these correlations were not significant in Landes geese; and iv) the activity of LPL had significant positive correlation with the proportions of lipids in subcutaneous adipose tissue and abdominal adipose tissue in Sichuan white geese, while in Landes geese the correlation was negative (p<0.05) with proportions of lipids in the liver, LPL activity had a significant positive correlation with the proportions of lipids in subcutaneous adipose tissue. These results suggest that the Landes geese have a better ability to use the massive amount of ingested food and to store lipids preferentially in the liver, but the Sichuan white geese have a relatively lower ability to use energetic nutrients and lipid storage is more efficient in the adipose tissues.

• Nutritional Sciences
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• 제1권1호
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• pp.8-15
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• 1998

In order to independently examine the effects of long-chain saturated fatty acids and dietary cholesterol levels on plasma and hepatic cholesterol concentrations, six different diets were fed to male Mongolian gerbils (14 gerbils pes grcup) for an 8-week period. Purified diets contained 36% energy as fat (each saturated fatty acid tested comprised about 20% of the total fat energy) and 0.06% (w/w) cholesterol, corresponding to typical human consumption patterns in Western diets. Fat blends were formulated with natural fat sources. To determine the effects of different saturated fatty acids on plasma and liver cholesterol levels, four of the six diets contained constant levels of all nutrients except for the amounts of lauric acid (12 0), myristic acid (14 : 0), palmitic acid (16 0), and stearic acid (18 : 0). Dietary cholesterol effects were tested using 16 : 0-enriched diets containing 0, 0.006, and 0.06% (w/w) cholesterol. None of the plasma lipids were influenced by fatty acid treatment, including triglycerides, plasma total-, VLDL+LDL-, and HDL-cholesterol. However, hepatic esterified cholesterol concentrations were increased in the palmitic and stearic arid diet groups compared to the lauric and myristic acid diet group. The molar ratios of hepatic EC/FC were the highest in the palmitic acid diet (12.2 $\pm$0.6) and the lowest in the myristic acid diet (6.4$\pm$0.2). Dietary cholesterol significantly (p<0.001) increased the plasma total cholesterol which was due to the increase of both HDL-and VLDL+LDL-cholesterol. In the absence of dietary cholesterol and compared to other species, the gerbil exhibited a high molar ratio of hepatic EC/FC, which was funker elevated by dietary cholesterol feeding (0.06%). The results from this study indicate that hepatic cholesterol concentrations are sensitive to both low levels of dietary cholesterol and saturated fatty acid chain length and also, that plasma cholesterol concentrations are sensitive to low levels of dietary cholesterol .

• Journal of Nutrition and Health
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• 제18권2호
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• pp.83-89
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• 1985

본 실험은 식이를 통한 콜레스테롤의 섭취가 흰쥐의 혈장 지단백 분획에 어떻게 영향하는지 또한 이때에 혈장 콜레스테롤 농도와 조직의 콜레스테롤 함량은 어떻게 변화하는지를 관찰하기 위하여 실시되었다. 총에너지의 12%를 옥수수기름으로 공급하는 식이에, 콜레스테롤 무첨가군을 대조군으로 하고 각각 0.2%, 0.5% 및 1.0%의 콜레스테롤 첨가군을 실험군으로 하였다. 상기 실험식이를 갓 이유된 Sprague-Dawley계통의 암쥐 및 숫쥐에게 4주간 자유급식시켰다. 실험결과 혈장 총 콜레스테롤 농도는 콜레스테롤 급여에 따른 영향을 나타내지 않았으나 지단백 분획은 유의한 반응을 나타내었다. 즉 식이 콜레스테롤의 급여 수준이 높을수록 ${\alpha}-lipoproteins$(HDL) 분획비는 점점 감소되었고 ${\beta}-lipoproteine$(VLDL+LDL) 분획비는 점차 증가되었다. 한편 조직의 콜레스테롤 함량은 간장과 골격근의 경우 유의한 증가를 보였다. 간장의 콜레스테롤 함량은 식이 콜레스테롤의 급여수준과 비례하여 증가하여 1.0% 첨가군의 경우 무첨가군에 비하여 약 7배의 축적량을 나타내었다. 그러나 골격근의 축적량은 약 1.5배 내지 1.9배 정도로 제한되었다. 반면 지방조직의 콜레스테롤 함량은 외인성 콜레스테롤의 섭취에 영향받지 않았다.