• Journal of Microbiology and Biotechnology
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• 제17권10호
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• pp.1733-1737
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• 2007

A total of 2,280 nonduplicate clinical isolates of Pseudomonas aeruginosa, obtained nationwide from Korean non-tertiary care hospitals from 2002 to 2005, were identified and their susceptibilities to aminoglycosides, antipseudomonal penicillins, carbapenems, cephalosporins, monobactams, and quinolones were studied, together with their production of ${\beta}$-lactamases. Using disk diffusion and minimum inhibitory concentration tests, it was found that 2.9% of isolates were multidrug-resistant (MDR) P. aeruginosa. An EDTA-disk synergy test, PCR amplification with specifically designed primers, and direct sequencing of the PCR products showed that the $bla_{OXA-10}$, $bla_{VIM-2}$, $bla_{OXA-2}$, $bla_{OXA-17}$, $bla_{PER-1}$, $bla_{SHV-12}$, and $bla_{IMP-1}$ genes were carried by 34.3%, 26.9%, 3.0%,3.0%, 1.5%, 1.5%, and 1.5% of 67 MDR P. aeruginosa isolates, respectively. The prevalence of MDR P. aeruginosa was three-fold higher, compared with that from the United States. More than two types of ${\beta}$-lactamase genes were carried by 10.4% of isolates. The most prevalent ${\beta}$-lactamase genes were $bla_{VIM-2}$ and $bla_{OXA-10}$. This study is the first description of MDR P. aeruginosa trom non-tertiary care hospitals in Korea and the coexistence of the $bla_{VIM-2}$, $bla_{IMP-1}$, or $bla_{PER-1} in these clinical isolates.

• 대한임상검사과학회지
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• 제49권4호
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• pp.413-419
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• 2017

폐렴의 한(일개) 환자의 임상검체에서 연속적으로 분리된 Imipenem 내성세균 4균주를 분리하였다. 분리균을 동정하기 위해 Vitek II system의 GN card를 이용하였으며 16S rRNA유전자 염기서열을 기초로 계통학적 분석을 실시하였다. 분리균은 P. aeruginosa (2 strains), P. monteilii (1) 및 P. putida (1) 으로 동정되었다. 분리균들의 항생제에 대한 내성시험은 Vitek II system AST-N225 card를 이용해서 imipenem의 최소억제 농도가 모두 $${\geq_-}8{\mu}g/mL$$을 확인한 후 실험에 사용하였다. ${\beta}-Lactamase$ 유전자의 특이 시발체를 이용하여 증폭한 PCR 산물로 imipenem 내성 유전자형을 결정하였는데 분리된 4균주 모두에서 MBL 유전자를 확인하였으며 2균주의 P. aeruginosa는 MBL유전자중 VIM형과 SHV형 유전자를 그리고 또다른 균주는 VIM형과 OXA group II형 유전자를 동시에 보유하고 있었다. 항생제 감수성 결과에서는 amikacin이 다른 항생제보다 감수성을 보였을 뿐 대체적으로 내성율이 높았다. 균주들간의 역학적 연관성 분석을 위해 ERIC-PCR을 이용한 DNA 지문 분석결과, 분리된 2 균주의 P. aeruginosa는 유사한 균주일 것으로 추정하였으나 DNA band 유형의 상동성은 서로 다른 유형임을 알아 볼 수 있었다. 특이하게 한 환자에게서 imipenem 내성세균이 4균주가 검출 된 것은 이례적이며 동종의 DNA band 유형도 서로 상이하였다.

• Journal of the Korean Data and Information Science Society
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• 제19권4호
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• pp.1371-1377
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• 2008

In this paper, we have employed the variational iteration method to solve the boundary value problems. Numerical results reveal that it is a very effective method compared with the results obtained by using the Adomian decomposition method in Wazwaz, A. M. (2000).

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 추계학술대회 논문집
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• pp.99-101
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• 2008

At 21st century, material development concepts were changed to fulfill the environmental friendly demands. This study is to study the effect of pressurized nitrogen gas and manganese in high nitrogen austenitic stainless steel(HNS) in which N and Mn elements substitute the nickel element. 100kg HNS ingots were made by Pressurized Vacuum Induction Melting(P-VIM) and were forged according to free forging process. As forged HNS were hot and cold rolled by pilot scale rolling machine. Depending on the rolling condition, the mechanical properties of HNS were changed. The roll thrust and sheet folding showed asymmetry condition between work and drive side during cold and hot rolling. The purpose of this study are to improve workability the hot and cold rolling machine and to set the conditions for establishing the rolling process.

• 한국정보통신학회논문지
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• 제15권11호
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• pp.2285-2291
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• 2011

이 논문에서는, 화면내 예측기, CAVLC(구문기반 적응가변길이 부호화기), DDR2 메모리 제어모듈을 집적화한 H.264 하이프로파일 화면내 부호화기를 제안한다. 설계된 부호화기는 한 매크로블록당 440 cycle에 동작할 수 있으며, 부호화기의 기능을 검증하기 위하여, JM13.2으로부터 참조 C 코드를 개발하고, 참조 C코드로부터 생성된 테스트벡터를 이용하여 개발된 하드웨어를 검증하였다. 개발된 부호화기는 FPGA에서 검증하였으며, DMA 는 200MHz에서, 부호화기모듈은 50MHz에서, 영상입력모듈(VIM)은 25MHz에서 동작한다. 회로의 크기는 Virtex 5XC5VLX330을 사용시에 약 20%의 LUT(43099개)를 사용하였다.

• 대한임상검사과학회지
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• 제43권2호
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• pp.68-74
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• 2011

Pseudomonas aeruginosa is an aerobic, Gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. Recently, outbreaks of carbapenem resistant P. aeruginosa give rise to significant therapeutic challenges for treating nosocomial infections. The genes of metallo-${\beta}$-lactamase (MBL), a powerful carbapenemase, are carried as a part of the mobile gene cassettes inserted into integrons playing an important role in rapid dissemination of antibiotic resistance genes among bacterial isolates. In this study, we investigated the prevalence of integron in imipenem resistant P. aeruginosa isolates. A total of 61 consecutive, non-duplicate, and imipenem resistant P. aeruginosa strains were isolated from a university hospital in the Chungcheong province of Korea. We employed repetitive extragenic palindromic sequence-based PCR (rep-PCR) method for the selection of clonally different P. aerusinosa strains. PCR and DNA sequencing were conducted for the detection of integrons. Twenty-one clonally different P. aeruginosa strains were isolated. Only one (P28) of the strains harbored $bla_{VIM-2}$ that was found as gene cassettes in class 1 integrons. Four of 21 carbapenem resistant P. aeruginosa strains harbored class 1 integron containing aminoglycoside resistance determinant. All of the integrons detected in the study contained more than one resistance gene cassette, which can mediate resistance to multiple antibiotics. To prevent further spreading of the multi-drug resistant P. aeruginosa, conseguent monitoring and clinical polices are required.

• 대한의생명과학회지
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• 제12권4호
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• pp.413-418
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• 2006

Charcot-Marie-Tooth disease (CMT) is blown as one of the inherited disorder of peripheral nervous system. Recently, it was found that point mutations in the neurofilament light subunit (NF-L) gene cause CMT. Neurofilaments (NFs) are heteropolymers consist of NF-L, NF-M and NF-H. To assess the relationship between CMT and NF-L mutation in cellular level, we performed phenotypic analysis of the mutant NF-L (E397K) using cultured cell lines. Vimentin-deficient human adrenal carcinoma SW13 (Vim-) cells have a potential to form the intermediate filaments when the cells are expressing both NF-L and NF-M. Our results show that co-expression of wild type NF-L with NF-M showed intermediate filament formation in SW13 (Vim-) cells, while E397K with NF-M did not. This result means that E397K mutant lost its ability to form the intermediate filament in vivo, and further suggests that the E397K mutation is closely related to CMT.

• International Journal of Naval Architecture and Ocean Engineering
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• 제7권5호
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• pp.906-919
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• 2015

The Deep Draft Semi-Submersible (DDS) concepts are known for their favourable vertical motion performance. However, the DDS may experience critical Vortex-Induced Motion (VIM) stemming from the fluctuating forces on the columns. In order to investigate the current-induced excitation forces of VIM, an experimental study of flow characteristics around four square-section cylinders in a square configuration is presented. A number of column spacing ratios and array attack angles were considered to investigate the parametric influences. The results comprise flow patterns, drag and lift forces, as well as Strouhal numbers. It is shown that both the drag and lift forces acting on the cylinders are slightly different between the various L/D values, and the fluctuating forces peak at L/D = 4.14. The lift force of downstream cylinders reaches its maximum at around ${\alpha}=15^{\circ}$. Furthermore, the flow around circular-section-cylinder arrays is also discussed in comparison with that of square cylinders.

• Nuclear Engineering and Technology
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• 제17권2호
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• pp.129-135
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• 1985

중성자 차폐계산에 미치는 칠의 공명에너지 영역에서의 미세군 및 소수군 구조에 의한 효과와 축약과정에서 사용되는 가중스펙트럼에 의한 효과를 ANISN 코드와 ENDF/B-IV자료로써 평가하였다. 다루어진 문제는 소수군효과, 철의 두께 증가에 의한 영향 및 고려된 계를 대상으로한 미상군계산에서 얻은 스펙트럼을 사용한 군축약효과이다. 이용된 군정수와 코드의 정확성을 검토하기 위해, 매우 정확한 계산정도를 갖고 있는 연속에너지 Monte Carlo코드 VIM에 의한 벤치마크계산을 수행하고 결과를 비교-검토하였다.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제16권10호
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• pp.1015-1019
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• 2010

문자열 완전일치 검색 알고리즘용 지금까지 많은 연구가 되어왔지만, EUC-KR 용 다중바이트 문자집합에 대해서는 연구원 것이 부족한 상황이다. 이 논문에서는 기존의 KMP 알고리즘을 사용할 때 EUC-KR과 같은 다중바이트 문자집합 텍스트에서 오검색이 발생할 수 있음을 보이며, 문자 단위의 접두사 함수를 적용하여 오검색이 발생하지 않도록 개선한 KMP 알고리즘을 제안한다. 또한, 널리 사용되고 있는 편집기인 Vim과 Emacs의 검색 알고리즘 및 기존의 오토마타 방식의 연구 결과에 비해 논문에서 제안한 알고리즘이 더 빠른 속도를 보이는 실험 결과를 제시한다.