• 대한두경부종양학회지
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• 제17권2호
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• pp.148-154
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• 2001

Objectives: This study was designed to investigate the significance of serum SCC antigen, CA 19-9, CA 125 level and DNA microsatellite alterations (MSA) as prognostic factors and indicators for recurrences in the pre-treatment and post-treatment state, respectively in head and neck cancer patients. Materials and Methods: 120 patients who received curative treatment for head and neck cancer from 1995 to 2000 were followed up successfully, and were analyzed retrospectively. Thirty healthy subjects served as normal controls. Serum SCC Ag levels were measured by microparticle enzyme immunoassay technique via IMX SCC assay, CA 19-9 levels were measured by CA 19-9 RIA test kit, and CA 125 levels were measured by CA 125 IRMA kit. MSA were identified after PCR amplification. Heterozygosity was considered lost if the ratio of one allele was significantly decreased (>50%) in serum DNA compared with normal DNA from lymphocytes. Results: Preoperative tumor markers were higher in cancer patients than control, but not significant. Postoperative SCC Ag levels were lower than preoperative levels. The SCC Ag levels were remained low in no evidence of disease (NED) group, but increased in locoregional recurrence and distant metastasis group. CA 19-9 and CA 125 levels showed no correlation between levels and recurrences and were not decreased significantly after primary tumor removal. MSA were detected in five out of 21 cases, and highly detected in distant metastasis group. Conclusion: SCC Ag seems to be a helpful serum tumor marker for early detection of recurrence and distant metastasis of head and neck cancer after curative treatment. But, CA 19-9 and CA 125 were not reliable markers for head and neck tumors. MSA were not statistically significant because of the small number of study group. However they may be helpful for screening serum molecular markers for early detection of distant metastasis of head and neck cancers.

• 대한두경부종양학회지
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• 제26권1호
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• pp.9-13
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• 2010

Background and Objectives : Parathyroid adenoma is a rare disease in Korea, but the incidence of parathyroid adenoma has gradually increased due to generalized measurement of serum calcium and imaging study according to wide spread public health screening program. In previous researches, the analysis of clinical aspects were insufficient due to a few cases. The purpose of this study was to assess the clinical analysis of surgically treated parathyroid adenoma with hyperparathyroidism. Methods : We reviewed the medical records of nineteen cases of parathyroid adenoma with hyperparathyroidism. Initial symptom to visit hospital, hypercalcemia associated medical symptom, surgical outcome and complication were investigated. Serum total calcium, parathyroid hormone, phosphate, alkaline phosphatase were checked before and after surgery. Imaging study was performed with combination of radionuclide parathyroid scan, ultrasonography and neck CT scan. Results : The initial symptoms were no symptom(6/19), pelvic pain(5/19), muscular weakness (3/19), bone pain(3/19) and palpable neck mass(2/19) in order of frequency. Serum total calcium, parathyroid hormone decreased and phosphate increased after surgery than before surgery statistically significantly. Sensitivities of parathyroid scan, neck ultrasonography and neck CT as preoperative localization test were 88.2%, 72.7%, 73.3% each. The most common postoperative complication was transient hypocalcemia(9/19). Conclusion : Similar to previous study, parathyroid adenomas have numerous clinical features and surgical treatment via unilateral approach with preoperatively localized single parathyroid adenoma was successful. In our study, parathyroid adenoma was predominantly detected by elevated serum calcium level with no clinical symptom so we need to evaluate parathyroid adenoma, if serum calcium elevated.

• 한국식품과학회지
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• 제52권6호
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• pp.670-677
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• 2020

본 연구에서는 가장 빈번하게 발생하는 세균성 식중독의 원인균인 C. jejuni의 주 감염원인 계육에 대하여, 식품공전에 등재된 식품첨가물 중 유화제를 이용하여 계육에서 C. jejuni의 부착을 제어 할 수 있는 기술을 확보하고자 하였다. 8종의 유화제를 200 mg/mL의 농도에서 paper disc agar diffusion method로 C. jejuni에 대한 항균활성을 검색한 결과 L-7D, L-1695, polysorbate 20, polysorbate 80 등 4종의 유화제에서 생육억제환을 생성하였다. L-7D, L-1695, polysorbate 20, polysorbate 80 4종의 유화제를 25, 50, 100, 200 mg/mL의 농도에서 항균활성을 검색한 결과, 농도가 작아질수록 생육억제환의 크기도 줄어들었으며, 유화제 중 L-1695 샘플이 200 mg/mL에서 가장 큰 생육억제환을 생성하였다. pH 및 열에 대해 안정성을 측정한 결과 L-7D, L-1695, polysorbate 20, polysorbate 80 4종의 유화제 모두 pH 및 열에 안정성을 가지고 있다는 것을 확인하였다. 최소저해농도를 측정한 결과 다른 유화제 L-7D, polysorbate 20, polysorbate 80 샘플과 비교하였을때 L-1695 샘플이 1.56 mg/mL에서 가장 좋은 최소저해농도를 나타냈다. 최소살균 효과는 L-7D, L-1695, polysorbate 20, polysorbate 80 4종의 유화제 모두 나타나지 않았다. 접촉표면의 부착제어능력을 확인하기 위해서 stainless steel과 ceramic에서 실험한 결과, 두 접촉 표면 모두에서 L-1695 샘플 처리 시 가장 적은 생균수를 나타냈다. 앞선 실험의 종합적인 결과에 따라 L-1695 유화제를 최종적으로 선별하고, 계육 피부에 부착된 C. jejuni에 영향을 주는지 CLSM으로 분석한 결과 대조구에 비하여 conventional spray 및 electrostatic spray를 처리하였을 때 모공에 부착된 균이 대다 수 떨어졌음을 확인하였다. 그러나 생균수를 확인해 본 결과 conventional spray와 electrostatic spray 처리 시에는 유의한 차이가 나타나지 않았다. 위 결과들을 종합한 결과 C. jejuni KCTC 5327에 대하여 L-1695 유화제는 생육을 억제시키지만, 살균효과는 없는 것을 확인하였다. 그러나 L-1695 유화제는 식품접촉표면에서 캠필로박터균의 부착을 저해하는 특성을 갖고 있으며, 계육 피부에 인위접종된 C. jejuni를 효과적으로 감소시키는 것을 볼 때, 실제 도계공정에서 세척수에 포함시켜 C. jejuni의 제어에 사용될 수 있을 것으로 기대된다.

• Korean Journal of Radiology
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• 제23권9호
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• pp.911-920
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• 2022

Objective: ⁶⁸Ga-NGUL is a novel prostate-specific membrane antigen (PSMA)-targeting tracer based on Glu-Urea-Lys derivatives conjugated to a 1,4,7-triazacyclononane-N,N',N''-triacetic acid (NOTA) chelator via a thiourea-type short linker. This phase I clinical trial of ⁶⁸Ga-NGUL was conducted to evaluate the safety and radiation dosimetry of ⁶⁸Ga-NGUL in healthy volunteers and the lesion detection rate of ⁶⁸Ga-NGUL in patients with prostate cancer. Materials and Methods: We designed a prospective, open-label, single-arm clinical trial with two cohorts comprising six healthy adult men and six patients with metastatic prostate cancer. Safety and blood test-based toxicities were monitored throughout the study. PET/CT scans were acquired at multiple time points after administering ⁶⁸Ga-NGUL (2 MBq/kg; 96-165 MBq). In healthy adults, absorbed organ doses and effective doses were calculated using the OLINDA/EXM software. In patients with prostate cancer, the rates of detecting suspicious lesions by ⁶⁸Ga-NGUL PET/CT and conventional imaging (CT and bone scintigraphy) during the screening period, within one month after recruitment, were compared. Results: All 12 participants (six healthy adults aged 31-32 years and six prostate cancer patients aged 57-81 years) completed the clinical trial. No drug-related adverse events were observed. In the healthy adult group, ⁶⁸Ga-NGUL was rapidly distributed, with the highest uptake in the kidneys. The median effective dose coefficient was calculated as 0.025 mSv/MBq, and cumulative activity in the bladder had the highest contribution. In patients with metastatic prostate cancer, 229 suspicious lesions were detected using either ⁶⁸Ga-NGUL PET/CT or conventional imaging. Among them, ⁶⁸Ga-NGUL PET/CT detected 199 (86.9%) lesions and CT or bone scintigraphy detected 114 (49.8%) lesions. Conclusion: ⁶⁸Ga-NGUL can be safely applied clinically and has shown a higher detection rate for the localization of metastatic lesions in prostate cancer than conventional imaging. Therefore, ⁶⁸Ga-NGUL is a valuable option for prostate cancer imaging.

• 한국안광학회지
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• 제18권2호
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• pp.125-136
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• 2013

목적: 본 연구는 설문을 통해 광과민성 난독증인 얼렌증후군(Meares-Ilren syndrome visual stress: MISViS)으로 진단을 받은 청소년을 대상으로 검사와 관련된 설문조사 및 각자 읽기에 도움이 되는 필터를 이용한 필터 사용 전, 후 읽기속도를 측정하여 읽기 속도 개선 효과를 비교분석하였다. 방법: 얼렌증후군 진단은 MISViS 선별설문결과에서 임상기준 점수(MISViS score) 2.13 이상으로 나오면 얼렌증후군이 있는 것으로 진단하였다. 읽기검사는 문맥이 맞지 않고, 내용이 없는 문장으로 구성된 검사지를 1분 동안 읽도록 하여 읽기 속도를 측정하였다. 15개의 컬러필터 그룹으로 구성되어 있는 필터 kit에서 각 그룹의 농도가 최하위인 필터를 선택하여 착용시킨 후 반응을 관찰하였다. 결과: 얼렌증후군 그룹의 MISViS score는 2.57이었고, 정상그룹의 MISViS score는 0.66이었다. 얼렌증후군 그룹의 필터착용 전과 후 읽기속도결과는 읽기속도(reading speed) $102.27{\pm}27.86(wpm)$에서 $118.87{\pm}26.99(wpm)$ (p=0.001), 정상그룹의 읽기속도는 $132.93{\pm}6.88(wpm$)에서 $133.43{\pm}6.64(wpm)$ (p=0.131)의 결과를 보였다. 두 그룹간의 필터 착용 전과 후의 오류변화 결과는 얼렌증후군 그룹이 줄생략(skipping)은 $0.25{\pm}0.62$회에서 0회(p=0.191), 단어잘못읽기(error)는 $1.83{\pm}1.69$회에서 $0.17{\pm}0.38$회 (p=0.004), 반복읽기(repetition)는 0회 이었다. 정상그룹의 줄생략(skipping)은 0회, 단어잘못읽기(error)는 $0.21{\pm}0.43$회에서 $0.07{\pm}0.27$회(p=0.336), 반복읽기(repetition)는 $0.14{\pm}0.36$회에서 0회 (p=0.165)의 결과를 보였다. 얼렌증후군 15명에서 선택된 컬러 필터의 색은 청색계열 컬러 필터의 선택비율이 40%로 가장 높게 나타났고, 정상대조군 14명에서 선택된 컬러 필터는 회색계열 컬러 선택비율이 29%로 가장 높게 나타났다. 결론: 본 연구 결과는 MISViS score가 얼렌증후군을 선별하는 기준으로 적용될 수 있음을 보여주고 있다. 얼렌증후군으로 진단받은 청소년들은 각자에 적합한 컬러필터를 착용 했을 때 읽기 개선 효과에 도움이 되는 것으로 나타났다. 얼렌증후군에서 컬러필터 선택은 개인에 적합한 고유의 컬러필터 파장이 서로 다르므로 선택을 신중하게 해야 할 것으로 사료된다.