• Title/Summary/Keyword: V79

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Vibration-Vibration Energy Transfer between $H^{79}Br\;and\;H^{81}Br$ ($H^{79}Br$$H^{81}Br$간의 진동 ${\to}$ 진동에너지 이동)

  • Chang Soon Lee;Yoo Hang Kim;Hyung Kyu Shin
    • Journal of the Korean Chemical Society
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    • v.28 no.6
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    • pp.361-365
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    • 1984
  • The long-lived collision model has been applied to the resonant vibration-vibration energy exchange process $H^{79}$Br(v = 1) + $H^{81}Br$(v = 0) ${\to}$ $H^{79}Br$(v = 0) + $H^{81}Br$(v = 1) + ${\Delta}E\;=\;0.38 cm^{-1}$ The energy exchange probabilities have been calculated over the temperature range from 200 to 800K. They show negative temperature dependence (P ${\propto}\;T^{-1.8}$) and agree with the available experimental data better than those calculated from other theories.

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Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress (효소처리에 의한 백삼 저분자 화합물의 V79-4 세포주에 대한 항산화 활성)

  • Kim, Young-Chan;Yim, Joo-Hyuk;Rho, Jeong-Hae;Cho, Chang-Won;Rhee, Young-Kyung
    • Journal of Ginseng Research
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    • v.31 no.4
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    • pp.203-209
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    • 2007
  • This study examined the extraction yields, total phenolic compounds content and the antioxidant activities on V79-4 cells of white ginseng extracts prepared by enzyme treatment. Yields of crude extract were 29.5-76%, and total phenolic compounds content showed 0.45-2.2% according to enzyme treatments. Pectinase treatment group showed the highest values of extraction yields and total phenolic compounds content. Pectinase and a-amylase treatment groups protected V79-4 cell viability(above 50%) against $H_2O_2$-induced oxidative damage. In the result of antioxidant enzyme activity evaluation in cells, enzyme treatments did not show the significant difference of SOD activity (p>0.05). However, pectinase treatment group exhibited increased CAT and GPx activities (p>0.05). Also, pectinase and protease treatment group inhibited MDA formation (>50%) in the lipid peroxidation protection experiment.

Screening of Natural Herb Methanol Extracts for Antioxidant Activity in V79-4 cells (천연 허브 메탄올 추출물의 V79-4 세포에서 항산화 활성 검색)

  • Chang, Jeong-Hwa;Yoo, Kyung-Mi;Hwang, In-Kyeong
    • Korean journal of food and cookery science
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    • v.22 no.4 s.94
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    • pp.428-437
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    • 2006
  • To investigate the worth of herbs as functional food ingredients, the antioxidant activity of 15 kinds of herb mathanol extracts was evaluated. Green tea, chamomile, dandelion, and lemon vervena extracts, with IC$_{50}$ values of 1.45 g/100mL, 1.49 g/100mL, 1.50 g/100mL and 1.55 g/100mL, respectively, had significantly higher superoxide radical scavenging activity than any other herb extracts. Green tea and lemon vervena extracts, which had high radical scavenging activity, showed inhibition of cell proliferation in Chinese hamster lung fibroblasts (V79-4 cells). Most herb extracts, except for chamomile, fennel and dandelion enhanced cell viability against H$_2$O$_2$-induced oxidative damage in V79-4 cells. At a dose of 1600 ${\mu}$g/mL, lemon vervena, green tea, hawthorn and rosemary extracts showed a cell viability of more than 50% which was significantly higher than that of the control culture treated with only H$_2$O$_2$ Thus, the results suggest that some herb extracts exhibited a V79-4 cell protective effect. The investigation of the cellular antioxidant enzymes activities of the five selected herb extracts revealed that extracts of lemon vervena and chamomile dose-dependently increased superoxide dismutase and glutathione peroxidase activity but that this increase was not significant. In conclusion, some natural herb extracts exhibited high antioxidant activity.

Antimutagenic Activities of the Germinated Specialty Rices in E. coli and V79 Cultured Cell Assay Systems (E. coli와 V79 배양세포계에서 발아특수미의 항돌연변이 활성)

  • Kang, Mi-Young;Nam, Seok-Hyun
    • Applied Biological Chemistry
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    • v.48 no.3
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    • pp.222-227
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    • 2005
  • To evaluate the antimutagenic activity of the specialty rices, a giant embryonic rice and a pigmented rice, we measured the inhibitory effect on the chemically induced mutagenesis in E. coli and V79 cultured cell system, as well as on DNA strand scission induced by oxidative damages in vitro. When the inhibitory activity to mitomycin C-induced mutagenesis using SOS chromotest in E. coli cell was measured, the activities decreased in the following order: germinated pigmented rice (40.4%) > germinated giant embryonic rice (37.1%) > pigmented rice (35.5%) > germinated brown rice (15.7%) > giant embryonic rice (14.0%) > brown rice (0.8%). The activities for inhibiting mitomycin C-induced DNA strand scission decreased in the order of pigmented rice > giant embryonic rice > germinated pigmented rice > germinated brown rice > brown rice > germinated giant embryonic rice. We also determined antimutagenic activities of the specialty rices using the suppressing effect on 6-TG resistant colony formation by 4-NQO in V79 cells as a mutagenicity index. The order of antimutagenicity was germinated giant embryonic rice (53.2%) > pigmented rice (40.0%) > brown rice (21.2%) > germinated brown rice (14.4%) > giant embryonic rice (0.23%); in contrast, germinated pigmented rice showed promoting effect on 4-NQO-induced mutagenesis.

Comparison of Micronulcleus Induction of Cigarette Smoke Condensate in Various Cell Lines (세포주에 따른 담배연기응축물의 소핵생성 비교)

  • 신한재;손형옥;이영구;이동욱;현학철
    • Journal of the Korean Society of Tobacco Science
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    • v.25 no.2
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    • pp.128-136
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    • 2003
  • Although tobacco smoke has been known to have genotoxicity as well as cytotoxicity, the sensitivity of the cell lines used against cigarette smoke is poorly understood. The objective of this study was to evaluate and compare the genotoxicity of several cell lines, which are routinely used in the in vitro assays, with cigarette smoke condensate(CSC) of Kentucky Reference Cigarette 1R4F. In the micronucleus(MN) induction assays, murine(CHO-K1, V79, BALB/c 3T3) cell lines and human(MCF-7, A549) ones were used. As a result, the CSC exhibited cytotoxicity with a concentration-dependent response in all cell lines. EC$_{50}$ of CSC in CHO-K1, V79, BALB/c 3T3, MCF-7 and A549 were 140, 125, 100, 116 and 109 $\mu\textrm{g}$/mL, respectively. On the other hand, the spontaneous micronucleated cell(MNC) frequency was stable and reproducible in every cell lines tested in this study. The dose-response of various cell lines to the induction of MN by CSC was estimated using linear regression analysis. CSC(0~100 $\mu\textrm{g}$/mL) caused a dose-dependent MN induction in CHO-K1, V79, BALB/c 3T3 and MCF-7 cell lines. Putting together all the data obtained and linear regression analysis of the data, we concluded that V79 cells are more susceptible to the accurate assessment of CSC-induced MN than the others.s.

AMPLIFICATION OF MERCURY TOXICITY BY GLUTATHIONE DEPLETION IN V79 CELLS

  • Yisook Nam;Chung, An-Sik
    • Toxicological Research
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    • v.9 no.2
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    • pp.159-166
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    • 1993
  • The treatmene of V79 cells with diethyl maleate (DEM) led to decrease in glutathione (GSH) level as increasing DEM concentration. Mercuric chloride, treated for 6 hrs with 2ng/ml, affected the GSH metabolizing enzymes glutathione S-transferase (GST) and glutathione peroxidase (GSP), dropping their activities to 60% and 75%, respectively, though not so much in GSH level(80%). However, the toxic effects of mercuric chloride on those enzymes and GSH level were both amplified when the Hg2+ treatment was combined with the preceding DEM treatment.

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