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Effective Production of $\beta$-Glucan by the Liquid Cultivation of Agaricus blazei (Agaricus blazei 균사체 배양기술을 통한 효율적인 $\beta$-glucan의 생산)

  • 이승현;임환미;김태영;조남석;박준성;유연우;김무성
    • Korean Journal of Microbiology
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    • v.40 no.1
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    • pp.54-59
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    • 2004
  • $\beta$-Glucan has been efficiently produced with higher yield by the optimization of liquid cultivation conditions. The optimal composition of medium for batch culture was 5% (w/v) of glucose as a carbon source, 0.5% (w/v) of yeast and 0.5% (w/v) of malt extract as a nitrogen source, 0.1% (w/v) of $KH_2PO_4$ and 0.05% (w/v) $MgSO_4{\cdot}7H_2O$, which had been the base medium for determination of other conditions. The set-up conditions are pH 5.0, $28^{\circ}C$, 1 vvm for aeration and 300 rpm for agitation. In order to minimize the inhibition effect of glucose on the initial growth of mycelia and to maximize the production of extracellular $\beta$-glucan, we have reduced the initial glucose feed to 4% and added 2nd feed at the point of 70 hr from the initial feed. The 2nd feed was composed of glucose 3%, yeast extract 0.1 % and malt extract 0.1 %. It improved the $\beta$-glucan yield upto 5.2 g/L in comparison with 2.8 g/L resulted from batch cultivation. Moreover, the serial treatment of a cell wall lytic enzyme and bromelain to the mycelia was effective for extraction of the cell wall bound $\beta$-glucan. The yield of $\beta$-glucan extraction by the enzyme treatment was 3.5 g/L, which was almost 4 times higher than that by hot-water extraction.

Sorbitol production from Jerusalem artichoke by inulinase and permeabilized Zymomonas mobilis (Inulinase와 투과성이 향상된 Zymomonas mobilis를 이용한 Jerusalem artichoke로 부터의 sorbitol생산)

  • 김인철;전억한
    • KSBB Journal
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    • v.7 no.1
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    • pp.15-20
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    • 1992
  • The use of Jerusalem artichoke containing $\beta$-1, 2-fructose oligomer in the production of sorbitol that is used as food additives and precursor for the L-sorbose has been studied. Coimmobilization of both inulinase and oxidoreductase was considered for the simultaneous reaction for hydrolysis of inulin and conversion of glucose and fructose liberated from inulin to sorbitol. Both inulinase and oxidoreductase were immobilized in chitin(5%, w/v) and K-carrageenan(4%, w/v), The activity of oxidoreductase was specified by permeabilization of Zymomonas mobilis cell with 0.2% CTAB(Cetyltrimethylammonlumbromide). The use of inulinase for hydrolysis of inulin resulted in 36.65g/l of glucose and 85.32g/1 of fructose respectively. These are valuable substrates for sorbitol production. Using these hydrolyzates, accumulation of 35.64g/l for sorbitol occurred at $38^{\circ}C$ and pH6.2. When permeabilized cells and inulinase were coimmobilized, sorbitol produced at 30.15g/l although it is low compared with 35.64g/l in separated reactor system.

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Optimization of Capacitor Threshold VT Implantation for Planar P-MOS DRAM Cell (평면구조 P-MOS DRAM 셀의 커패시터 VT 이온주입의 최적화)

  • Chang Sung-Keun;Kim Youn-Jang
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.19 no.2
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    • pp.126-129
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    • 2006
  • We investigated an optimized condition of the capacitor threshold voltage implantation(capacitor $V_T$ Implant) in planar P-MOS DRAM Cell. Several samples with different condition of the capacitor $V_T$ Implant were prepared. It appeared that for the capacitor $V_T$ Implant of $BF_2\;2.0{\times}l0^{13}\;cm^{-2}$ 15 KeV, refresh time is three times larger than that of the sample, in which capacitor $V_T$ Implant is in $BF_2\;1.0{\times}l0^{13}\;cm^{-2}$ 15 KeV. Raphael simulation revealed that the lowed maximum electric field and lowed minimum depletion capacitance ($C_{MIN}$) under the capacitor resulted in well refresh characteristics.

A Study on Microstructure and Mechanical Properties of Hf, Ta Added Ti-l5Sn-4Nb system Alloys for Biomaterial (Hf, Ta가 첨가된 Ti-l5Sn-4Nb계 생체용 합금의 미세조직 및 기계적 성질에 관한 연구)

  • 김대환;이경구;박효병;이도재
    • Journal of the Korean institute of surface engineering
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    • v.33 no.4
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    • pp.251-260
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    • 2000
  • Ta and Hf added Ti-l5Sn-4Nb alloys without V and Al elements for biomaterial were melted by arc furnace in response to recent concerns about the long term safety of Ti-6Al-4V alloy. All specimens were homogenized at $1000^{\circ}C$ and solution treatment was performed at $812^{\circ}C$ and aging treatment at $500^{\circ}C$. The microstructure and mechanical properties were analysed by optical micrograph, hardness tester and instron. Ti-l5Sn-4Nb system alloys showed widmanstatten microstructure which is typical microstructure in $\alpha$$\beta$ type Ti alloys. The Ti-l5Sn-4Nb-2Hf and Ti-l5Sn-4Nb-2Ta alloys showed better hardness and tensile strength compared with Ti-6Al-4V. The result of XPS analysis, Ti-l5Sn-4Nb alloy in air atmosphere consisted of $TiO_2$, SnO and NbO.

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Hybrid Water Treatment of Tubular Ceramic MF and Photocatalyst Loaded Polyethersulfone Beads : Effect of Organic Matters, Adsorption and Photo-oxidation at Nitrogen Back-flushing (관형 세라믹 정밀여과와 광촉매 첨가 PES 구의 혼성 수처리 : 질소 역세척 시 유기물 및 흡착, 광산화의 영향)

  • Hong, Sung Taek;Park, Jin Yong
    • Membrane Journal
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    • v.23 no.1
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    • pp.61-69
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    • 2013
  • The effect of humic acid (HA), and the roles of microfiltration (MF), PES (polyethersulfone) beads adsorption, and photo-oxidation were investigated in hybrid process of ceramic MF and PES beads loaded with titanium dioxide ($TiO_2$) photocatalyst for advanced drinking water treatment. Those were compared and studied in viewpoints of membrane fouling resistance ($R_f$), permeate flux (J), and total permeate volume ($V_T$). Because membrane fouling increased dramatically as decreasing HA, $R_f$ increased and J decreased, and finally $V_T$ was the highest at 2 mg/L HA. In the experiment to investigate the roles of photo-oxidation and adsorption at humic acid 4 mg/L and 6 mg/L. In both conditions, $R_f$ was the lowest and $V_T$ was the highest in MF + $TiO_2$ + UV process. The average treatment efficiencies of turbidity and dissolved organic matters were the highest in MF + $TiO_2$ + UV process, too.

INJECTIVELY DELTA CHOOSABLE GRAPHS

  • Kim, Seog-Jin;Park, Won-Jin
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.4
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    • pp.1303-1314
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    • 2013
  • An injective coloring of a graph G is an assignment of colors to the vertices of G so that any two vertices with a common neighbor receive distinct colors. A graph G is said to be injectively $k$-choosable if any list $L(v)$ of size at least $k$ for every vertex $v$ allows an injective coloring ${\phi}(v)$ such that ${\phi}(v){\in}L(v)$ for every $v{\in}V(G)$. The least $k$ for which G is injectively $k$-choosable is the injective choosability number of G, denoted by ${\chi}^l_i(G)$. In this paper, we obtain new sufficient conditions to be ${\chi}^l_i(G)={\Delta}(G)$. Maximum average degree, mad(G), is defined by mad(G) = max{2e(H)/n(H) : H is a subgraph of G}. We prove that if mad(G) < $\frac{8k-3}{3k}$, then ${\chi}^l_i(G)={\Delta}(G)$ where $k={\Delta}(G)$ and ${\Delta}(G){\geq}6$. In addition, when ${\Delta}(G)=5$ we prove that ${\chi}^l_i(G)={\Delta}(G)$ if mad(G) < $\frac{17}{7}$, and when ${\Delta}(G)=4$ we prove that ${\chi}^l_i(G)={\Delta}(G)$ if mad(G) < $\frac{7}{3}$. These results generalize some of previous results in [1, 4].

Effect of Fused Superphosphate Levels in Various Root Media Containing Micronutrient Mixes on Growth of Marigold Plug Seedlings (미량원소복합제가 혼합된 각종 상토에서 용과린의 시비수준이 매리골드 플러그 묘의 생육에 미치는 영향)

  • Choi Jong-Myung
    • Journal of Bio-Environment Control
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    • v.15 no.2
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    • pp.196-203
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    • 2006
  • Objective of this research was to determine the effect of application level of fused superphosphate (FSPP) in root media containing granular micronutrient fertilizers (MF) on growth of marigold 'Orange boy' in plug culture. To achieve this, three granular micronutrient mixes such as MF 1, MF 2, and MF 3 were produced and incorporated into three root media, peatmoss+composted pine bark (1:1, v/v, PB), peatmoss+composted rice-hull (1:1, v/v, PR), and peatmoss+composted saw-dust (1:1, v/v, PS), at a rate of $0.3g{\cdot}L^{-1}$. Elevated application level of FSPP in PS medium containing each of MF resulted in increase of plant growth such as plant height, plant width, stem diameter, fresh weight and dry weight at 35days after sowing. The dry weight in the treatment of MF 1, MF 2, MF 3, and control fertilized with $9.0g{\cdot}L^{-1}$ of FSPP were 0.066g, 0.103g, 0.077g, and 0.095g per plant, respectively. These results indicated that each of MF affected marigold plug seedlings differently. The application level of FSPP resulted in the heaviest fresh and dry weight in PR medium were $9.0g{\cdot}L^{-1}$ in MF 1, $3.0g{\cdot}L^{-1}$ in MF 2, $9.0g{\cdot}L^{-1}$ in MF 3, and $6.0g{\cdot}L^{-1}$ in control. Elevated application level of FSPP in PB medium containing each of the micronutrient mix increased fresh and dry weight. The general trends in response of plant growth to elevated FSPP were linear and quadratic. The dry weight in the treatment of MF 1, MF 2, MF 3, and control fertilized with $9.0g{\cdot}L^{-1}$ of FSPP in PB medium were 0.131g, 0.104g, 0.137g, and 0.111g per plant at 35days after sowing. These results indicated that MF 1 and MF 3 performed better than MF 2 and control treatments in growth of marigold plug seedlings.

Population Pharmacokinetics for Gentamicin in Korean and Caucasian Appendicitis Patients Using Nonparametric Expected Maximum (NPEM) Algorithm (한국인과 코카시안 충수돌기염 환자에서 비모수적 기대최대치(NPEM) 연산방법에 의한 겐타마이신의 모집단 약물동태학)

  • Burm, Jin-Pil
    • Korean Journal of Clinical Pharmacy
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    • v.21 no.2
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    • pp.74-80
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    • 2011
  • Population pharmacokinetics for gentamicin were compared with 20 Korean patients (14 male and 6 female) and 25 Caucasian appendicitis patients (16 male and 9 female). Two to six blood specimens were collected from all patients at the following times : just before a regularly scheduled infusion and at 0.5 hour after the end of a 0.5 hour infusion. Nonparametric expected maximum(NPEM) algorithm for population modeling was used. The estimated parameters were the elimination rate constant(K), the slope(KS) of the relationship between K versus creatinine clearance(Ccr), the apparent volume of distribution (V), the slope(VS) of the relationship between V versus weight, gentamicin clearance(CL) and the slope(CS) of the relationship between CL versus Ccr and the V. The output includes two marginal probability density function(PDF), means, medians, modes, variance, skewness, kurtosis, and CV%. The mean K(KS) were$0.402{\pm}0.129hr^{-1}$ ($0.00486{\pm}0.00197[hr{\cdot}mL/min/1.73m^2]^{-1}$) and $0.425{\pm}0.137hr^{-1}$($0.00432{\pm}0.00168[hr{\cdot}mL/min/1.73m^2]^{-1}$) for Korean and Caucasian populations, respectively. The mean V(VS) were not different at $14.3{\pm}3.69L$($0.241{\pm}0.0511L/kg$) and $15.8{\pm}4.81L$($0.236{\pm}0.0531L/kg$) for Korean and Caucasian populations, respectively (P>0.2). The mean CL(CS) were $5.68{\pm}1.69L/hr$ ($0.0714{\pm}0.0222L/kg[hr{\cdot}mL/min/1.73m^2]$) and $6.29{\pm}1.84L/hr$ ($0.0629{\pm}0.0189L/kg[hr{\cdot}mL/min/1.73m^2]$) for Korean and Caucasian populations, respectively. There are no differences in gentamicin pharmacokinetics between Korean and Caucasian appendicitis patients.

Mechanistic Investigation of Redox Process of 2-Amino-1-cyclopentene-1-dithiocarboxylate derivatives (2-Amino-1-cyclopentene-1-dithiocarboxylate 유도체들의 전극 반응메카니즘)

  • Kim, Yeong Sin;Kim, U Seong;Sim, Yun Bo;Choe, Seong Rak
    • Journal of the Korean Chemical Society
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    • v.34 no.3
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    • pp.260-266
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    • 1990
  • The electrochemical behavior of N-methyl-2-amino-l-cyclopentene-l-dithiocarboxylic acid $(N-CH_3 acdc) and 2-amino-l-cyclopentene-l-dithiomethyl ester (S-CH_3 acdc) in DMF have been investigated by the use of polarography, cyclic voltammetry and coulometry. The dimer of N-CH_3 acdc is further oxidized at +0.98 V via 2-electron process to produce free sulfur atom and cyclization product. The ring formation between two dithio group occurs along with the elimination of one sulfur atom. The elimination of sulfur atom occurs via two electron oxidation process at + 0.98 V vs. Ag/AgCl electrode. However, the cyclization does not occur in the S-CH_3$ acdc.

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Synthesis and Characterization of a Di-$\mu$-oxo-bridged Molybdeum(V) Complexes (두 개 산소가교형 몰리브덴(V) 착물의 합성과 그 성질에 관한 연구)

  • Doh, Gil Myung;Kim, Ill Chool;Choi, Bo Yong
    • Journal of the Korean Chemical Society
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    • v.39 no.3
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    • pp.198-203
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    • 1995
  • The Mo(V) $di-\mu-oxo$ type $(Mo_2O_4(H_2O)_2L)$ complexes $(L:\;C_3H_7CH(SCH_2COOH)_2,\;C_6H_5CH(SCH_2COOH)_2,\;CH_3OC_6H_4CH(SCH_2COOH)_2,\;C_5H_{10}C(SCH_2COOH)_2,\;C_3H_7C(CH_3)(SCH_2COOH)_2,\;C_3H_7CH(SCH_2CH_2COOH)_2,\;C_6H_5CH(SCH_2CH_2COOH)_2)$ have been prepared by the reaction of $[Mo_2O_4(H_2O)_6]^{2+}$ with a series of dithiodicarboxy ligands. These complexes are completed by two terminal oxygens arranged trans to one another and each ligand forms a chelate type between two molybdenum. In $Mo_2O_4(H_2O)_2L$, two $H_2O$ coordinated at trans site of terminal oxygens. The prepared complexes have been characterized by elemental analysis, infrared spectra, electronic spectra, and nuclear magnetic resonance spectra. In the potential range -0.00 V to -1.00 V at a scan rate of 20 $mVs^{-1}$, a cathodic peak at -0.50∼-0.58 V (vs. SCE) and an anodic peak at -0.40∼-0.43 V (vs. SCE) have been observed in aquous solution. The ratio of the cathodic to anodic current ($I_{pc}/I_{pa}$) is almost 1, we infer that redox is reversible reaction.

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