• Title/Summary/Keyword: Utilization of Amino Acids

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The Annual Reproductive Cycle, Proximate Composition, Fatty Acid and Amino Acid Content of Pacific Oyster, Crassostrea gigas (Magallana gigas), in Gadeok-do, Korea

  • In Kyu Cho;Bong-Seung Seo;So-Yeon Hwang;Ye-In Lee;Ji-Sung Moon;Su-Jin Park;Hee-Jung Lee;Young Baek Hur;Youn Hee Choi
    • Development and Reproduction
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    • v.27 no.3
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    • pp.101-115
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    • 2023
  • Environmental factors impact oyster growth, condition, and gonadal development, which is linked to gamete characteristics observed through histology. The reproductive cycle of bivalves is related to energy storage and utilization. Therefore, in this study, the year-round growth change and gonadal development of oysters were observed using histological analysis, and the biochemical composition changes were confirmed. The oysters used in this study are being nurtured in Gadeok-do, and 40 oysters were randomly sampled monthly from March 2021 to February 2022. Result of histological analysis of gonads, oysters were showed early development from December to February, late development from March and April, mature and ripe from May to July, spawned from August to October, and spent from November to December. Condition index values of oysters decreased in summer and autumn and increased again when entered the spent after spawning. The protein content of oysters was high in May, the maturity period, and the lipid content decreased during the spawning period. In addition, EPA and DHA, the major fatty acids of oysters, were low during the spawning period and high during the maturation period. As a result, this study suggested a close relationship between changes in oyster growth, biochemical composition, and the reproductive cycle.

Effect of Feed Protein Source on Digestion and Wool Production in Angora Rabbit

  • Bhatt, R.S.;Sawal, R.K.;Mahajan, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.7
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    • pp.1075-1079
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    • 1999
  • Adult German cross $(German{\times}British{\times}Russian)$ angora rabbits (one year age), 32 in number were divided randomly into four groups $(T_1-T_4)$ with equal sex ratio and fed diets containing $T_1$ groundnut cake (GNC); $T_3$, soyaflakes (SF); $T_4$, sunflower cake (SFC) and $T_2$, a mixture of all the three cakes along with green forage as roughage for a period of 9 months. Nine per cent protein was added from each protein source. Fibre level was maintained by adjusting the level of rice phak in the diets. The diets were iso-nitrogenous and contained similar level of fibre. DMI through roughage was not affected due to source of protein in the diet, however, DMI through concentrate was higher $(p{\leq}0.05)$ with SFC diet, which resulted in higher total feed intake in the group $(T_4)$. Body weights increased up to second shearing, thereafter it decreased due to summer depression. Diet containing soyaflakes sustained higher wool yield whereas, it was lowest $(p{\leq}0.05)$ on SFC diet. Wool attributes (staple length, medullation, fibre diameter) were not affected due to source of protein in the diet. Digestibility of fibre and its fractions (ADF, cellulose, hemicellulose) decreased $(p{\leq}0.05)$ with incorporation of SFC in the diets. Balance of calcium was lowest whereas, that of nitrogen was highest with SFC diet $(T_4)$. Biological value of N and net protein utilization was better when different protein sources were mixed together $(T_2)$. Protein quality of soyaflakes proved better for wool production followed by groundnut cake and mixture of three protein sources. Sunflower cake alone or in combination decreased wool production which may be checked by supplementation of amino acids and energy.

Preparation of Fish Sauce from Mackerel Scrap (고등어 가공잔사를 이용한 어간장의 제조)

  • Lee, Eung-Ho;Park, Hyang-Suk;Ahn, Chang-Bum;Hwang, Gyu-Chul
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.15 no.3
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    • pp.201-206
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    • 1986
  • The purpose of this study is to prepare the fish sauce front mackerel scrap which usually comprises $40{\sim}50%$ of raw fish in processing. Mackerel scrap was chopped, mired with equal weight of water, and then hydrolyzed by autolysis. The optimal conditions for hydrolysis of .mackerel scrap were at $55^{\circ}C$ for 4 hours. The maximum hydrolyzed rate of protein was 65% by autolysis. Crude protein content (6.5%) and color of mackerel sauce were similar to those of traditional soybean sauce. The abundant amino acids in mackerel sauce were leucine (22.8%), isoleucine (15.0%), phenylalanine (12.6%) and valine (12.5%). In sensory evaluation, mackerel sauce was at least equal to the traditional soybean sauce in product quality.

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Effects of Rice Koji on the Fermentation Characteristics of the Sik-hae from Sailfin Sandfish Arctoscopus japonicus (쌀코지가 도루묵(Arctoscopus japonicus) 식해의 발효특성에 미치는 영향)

  • Jung, Min-Jeong;Nam, Jong-Woong;Han, Ah-Ram;Kim, Byoung-Mok;Jun, Joon-Young;Kim, Gwang-Woo;Lee, Mi-Hyang;Jeong, In-Hak
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.808-816
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    • 2022
  • This study was conducted with a view toward enhancing the industrial utilization of a male sailfin sandfish Arctoscopus japonicus based on its application in the preparation of Sik-hae, a Korean traditional fermented fishery product prepared using hard-boiled rice and enzymatic malt sprouts. As an alternative to rice and malt sprouts, we evaluated the utility of rice Koji for Sik-hae fermentation by investigating the physicochemical changes that occur during fermentation. The sailfin sand fish Sik-hae fermented with 10% or 20% rice Koji was found to show similar fermentation characteristics to the control fermentation with 20% hard-boiled rice and 4% malt sprouts, with respect to acid production, texture profile, and the softening of bones during the entire fermentation period. In particular, compared with the control, the addition of 10% rice Koji reduced the dehydration of Sik-hae and increased the content of free amino acids, which contributed to preventing a marked reduction in pH during fermentation. Accordingly, 10% rice Koji is proposed as a viable alternative enzymatic agent for the preparation of Sik-hae, which can contribute enhancing the desired properties of this traditional food product.

Characteristics and Applications of Bioactive Peptides in Skin Care (생리활성 펩타이드의 피부미용학적 특성 및 활용)

  • Moh, Sang-Hyun;Jung, Dai-Hyun;Kim, Hyoung-Shik;Cho, Moon-Jin;Seo, Hyo-Hyun;Kim, Sung-Jun
    • KSBB Journal
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    • v.26 no.6
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    • pp.483-490
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    • 2011
  • Bioactive peptides (BAP) showed excellent cosmetic activity than bio-materials such as caffeic acid (CA), gallic acid (GA), and nicotinic acid (NA). Caffeoyl tripeptide-1 (CT-1) is a BAP that is stabilized with Gly-His-Lys (GHK) tripeptide and CA by using Fmoc solid phase peptide synthesis. Digalloyl tetrapeptide-19 (DT-19) is stabilized by combining Lys-Glu-Cys-Gly with GA and nicotinoyl tripeptide-1 (NT-1) is synthesized by GHK and NA. According to experiments, CT-1 has an excellent anti-oxidant function even with a very small amount of 10 ppm CT-1. DT-19's tyrosinase inhibition activity has the better effect of about 28.57% in 0.01% and 33.33% in 0.005% of concentration and about 7.89% in 0.001% concentration than vitamin-C. In addition, NT-1 is safer than the NA. Almost BAPs like pal-KTTKS, acetyl hexapeptide, and copper tripeptide-1 have the anti-wrinkle effect while DT-19 and NT-1 are applicable for potential BAPs focused on the whitening effect. The three kinds of BAPs like CT-1, DT-19, and NT-1 consisting of amino acids are safe to the skin, and have more excellent stability than bio-materials which are found to be unstable and cause skin irritation. Due to the high biological activity of BAP in the field of skin care, its utilization will increase constantly.

Dietary inclusion of mealworm (Tenebrio molitor) meal as an alternative protein source in practical diets for rainbow trout (Oncorhynchus mykiss) fry

  • Jeong, Seong-Mok;Khosravi, Sanaz;Mauliasari, Intan Rizki;Lee, Sang-Min
    • Fisheries and Aquatic Sciences
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    • v.23 no.5
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    • pp.12.1-12.8
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    • 2020
  • Background: An 8-week feeding trial was designed to evaluate the potential of yellow mealworm (MW; Tenebrio molitor) as a locally available nutrient-rich feedstuff for rainbow trout fry (Oncorhynchus mykiss). Methods: Triplicate groups of fish (mean ± SE; 1.11 ± 0.01 g) were assigned to each of the five isonitrogenous and isocaloric practical diets containing graded level of a full fat MW (0, 7, 14, 21, and 28%) at the expense of fish meal (designated as MW0, MW7, MW14, MW21, and MW28, respectively). Results: Fish growth performance in terms of weight gain and specific growth rate significantly increased with increasing dietary MW level up to 14% and then declined when dietary MW levels further increased to 28%. Significantly higher protein efficiency ratio and lower feed conversion ratio were found in fish fed with diets containing MW compared to fish fed the control MW0. Myeloperoxidase activity was significantly higher in fish fed MW7 diet compared to fish fed the MW0 diet. Fish fed the MW14 and MW28 diets had significantly higher lysozyme activities than those fed the MW0 diet. Conclusions: Overall, the efficacy of MW as promising alternative to fish meal in practical diets for rainbow trout fry has been proved not only in relation to growth rates and feed utilization, but also from the viewpoint of immunopotentiation effects.

Preparation and Properties of Biodegradable Hydrogels from Poly(2-hydroxyethyl aspartamide) and HMDI (HMDI 가교 폴리아스팔트아미드 수화젤의 제조 및 특성)

  • Kim Jeong Hoon;Sim Sang Jun;Lee Dong Hyun;Kim Dukjoon;Lee Youngkwan;Kim Ji-Heung
    • Polymer(Korea)
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    • v.29 no.5
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    • pp.518-521
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    • 2005
  • Biodegradable polymers and hydrogels have been increasingly applied in a variety of biomedical applications including current drug delivery system and tissue engineering field. ${\alpha},\;{\beta}-Poly$(N-2-hydroxyethyl-DL-aspart-amide), PHEA. is one of poly(amino acids) with hydroxyethyl pendants, which is hewn to be biodegradable and potentially biocompatible. So that, the utilization and various chemical modifications of PHEA have been attempted for useful biomedical applications. In this wort chemical gels based on PHEA were prepared by crosslinking with diisocyanate compound in DMF in the presence of catalyst. Here, the PHEA was prepared from polysuccinimde, the thermal polycondensation product of aspartic acid, via ring-opening reaction with ethanolamine. The preparation of gels and their swelling behavior, depending on the different medium and pH, were investigated. Also the morphology by SEM and simple hydrolytic degradation were observed.

Manipulation of Cassava Cultivation and Utilization to Improve Protein to Energy Biomass for Livestock Feeding in the Tropics

  • Wanapat, M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.3
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    • pp.463-472
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    • 2003
  • Cassava (Manihot esculenta, Crantz), an annual tropical tuber crop, was nutritionally evaluated as a foliage for ruminants, especially dairy cattle. Cultivation of cassava biomass to produce hay is based on a first harvest of the foliage at three months after planting, followed every two months thereafter until one year. Inter-cropping of leguminous fodder as food-feed between rows of cassava, such as Leucaena leucocephala or cowpea (Vigna unculata), enriches soil fertility and provides additional fodder. Cassava hay contained 20 to 25% crude protein in the dry matter with good profile of amino acids. Feeding trials with cattle revealed high levels of DM intake (3.2% of BW) and high DM digestibility (71%). The hay contains tannin-protein complexes which could act as rumen by - pass protein for digestion in the small intestine. As cassava hay contains condensed tannins, it could have subsequent impact on changing rumen ecology particularly changing rumen microbes population. Therefore, supplementation with cassava hay at 1-2 kg/hd/d to dairy cattle could markedly reduce concentrate requirements, and increase milk yield and composition. Moreover, cassava hay supplementation in dairy cattle could increase milk thiocyanate which could possibly enhance milk quality and milk storage, especially in small holder-dairy farming. Condensed tannins contained in cassava hay have also been shown to potentially reduce gastrointestinal nematodes in ruminants and therefore could act as an anthelmintic agent. Cassava hay is therefore an excellent multi-nutrient source for animals, especially for dairy cattle during the long dry season, and has the potential to increase the productivity and profitability of sustainable livestock production systems in the tropics.

Dominance and Survival Strategy of Toxic Dinoflagellate Alexandrium tamarense and Alexandium catenella Under Dissolved Inorganic Nitrogen-limited Conditions (용존태 무기질소 제한 하에서 유독와편모조류 Alexandrium tamarense와 Alexandium catenella의 생존과 우점화 전략)

  • Kwon, Hyeong Kyu;Park, Ji A;Yang, Han-Soeb;Oh, Seok Jin
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.16 no.1
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    • pp.25-35
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    • 2013
  • Dominance and survival strategy of toxic dinoflagellate Alexandrium tamarense and A. catenella under the dissolved inorganic nitrogen (DIN) limited conditions were examined in the laboratory and field observations. In Masan Bay, DIN was limiting factor for growth of phytoplankton during spring to early summer when Alexandrium spp. have been observed. They have a disadvantageous position compared with diatoms because Ks of nitrate calculated from growth kinetics experiment of A. tamarense and A. catenella was higher than diatoms. However, A. tamarense and A. catenella were able to grow using dissolved organic nitrogen (DON) compounds such as urea and amino acids as well as DIN. Therefore, DON utilization of A. tamarense, A. catenella might contribute to not only their population growth but also dominance and interspecific competition in the DIN-limited conditions in Masan Bay.

A Study on the Nutritive Value and Utilization of Powdered Seaweeds (해조의 식용분말화에 관한 연구)

  • Yu, Jong-Yull;Lee, Ki-Yull;Kim, Sook-Hee
    • Journal of Nutrition and Health
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    • v.8 no.1
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    • pp.15-37
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    • 1975
  • I. Subject of the study A study on the nutritive value and utilization of powdered seaweeds. II. Purpose and Importance of the study A. In Korea the shortage of food will be inevitable by the rapidly growing population. It will be very important study to develop a new food from the seaweeds which were not used hitherto for human consumption. B. The several kinds of seaweeds have been used by man in Korea mainly as side-dishes. However, a properly powdered seaweed will enable itself to be a good supplement or mixture to certain cereal flours. C. By adding the powdered seaweed to any cereals which have long been staple foods in this country the two fold benefits; saving of cereals and change of dietary pattern, will be secured. III. Objects and scope of the study A. Objects of the study The objects will come under four items. 1. To develop a powdered seaweed as a new food from the seaweeds which have been not used for human consumption. 2. To evaluate the nutritional quality of the products the analysis for chemical composition and animal feeding experiment will be conducted. 3. Experimental cocking and accepability test will be conducted for the powdered products to evaluate the value as food stuff. 4. Sanitary test and also economical analysis will be conducted for the powdered products. B. Scope of the study 1. Production of seaweed powders Sargassum fulvellum growing in eastern coast and Sargassum patens C.A. in southern coast were used as the material for the powders. These algae, which have been not used for human consumption, were pulverized through the processes of washing, drying, pulverization, etc. 2. Nutritional experiments a. Chemical composition Proximate components (water, protein, fat, cellulose, sugar, ash, salt), minerals (calcium, phosphorus, iron, iodine), vitamins (A, $B_1,\;B_2$ niacin, C) and amino acids were analyzed for the seaweed powders. b. Animal feeding experiment Weaning 160 rats (80 male and 80 female rats) were used as experimental animals, dividing them into 16 groups, 10 rats each group. Each group was fed for 12 weeks on cereal diet (Wheat flour, rice powder, barley powder, potato powder, corn flour) with the supplementary levels of 5%, 10%, 15%, 20% and 30% of the seaweed powder. After the feeding the growth, feed efficiency ratio, protain efficiency ratio and ,organs weights were checked and urine analysis, feces analysis and serum analysis were also conducted. 3. Experimental cooking and acceptability test a. Several basic studies were conducted to find the characteristics of the seaweed powder. b. 17 kinds of Korean dishes and 9 kinds of foreign dishes were prepared with cereal flours (wheat, rice, barley, potato, corn) with the supplementary levels of 5%, 10%, 15%, 20% and 30% of the seaweed powder. c. Acceptability test for the dishes was conducted according to plank's Form. 4. Sanitary test The heavy metals (Cd, Pb, As, Hg) in the seaweed powders were determined. 5. Economical analysis The retail price of the seaweed powder was compared with those of other cereals in the market. And also economical analysis was made from the nutritional point of view, calculating the body weight gained in grams per unit price of each feeding diet. IV. Results of the study and the suggestion for application A. Chemical composition 1. There is no any big difference in proximate components between powders of Sargassum fulvellum in eastern coast and Sargassum patens C.A. in southern coast. Seasonal difference is also not significant. Higher levels of protein, cellulose, ash and salt were found in the powders compared with common cereal foods. 2. The levels of calcium (Ca) and iron (Fe) in the powders were significantly higher than common cereal foods and also rich in iodine (I). Existence of vitamin A and vitamin C in the Powders is different point from cereal foods. Vitamin $B_1\;and\;B_2$ are also relatively rich in the powders.'Vitamin A in ·Sargassum fulvellum is high and the levels of some minerals and vitamins are seemed4 to be some influenced by seasons. 3. In the amino acid composition methionine, isoleucine, Iysine and valine are limiting amino acids. The protein qualities of Sargassum fulvellum and Sargassum patens C.A. are seemed to be .almost same and generally ·good. Seasonal difference in amino acid composition was found. B. Animal feeding experiment 1. The best growth was found at.10% supplemental level of the seaweed Powder and lower growth rate was shown at 30% level. 2. It was shown that 15% supplemental level of the Seaweed powder seems to fulfil, to some extent the mineral requirement of the animals. 3. No any changes were found in organs development except that, in kidney, there found decreasing in weight by increasing the supplemental level of the seaweed powder. 4. There is no any significant changes in nitrogen retention, serum cholesterol, serum calcium and urinary calcium in each supplemental level of the seaweed powder. 5. In animal feeding experiment it was concluded that $5%{\sim}15%$ levels supplementation of the seaweed powder are possible. C. Experimental cooking and acceptability test 1. The seaweed powder showed to be utilized more excellently in foreign cookings than in Korean cookings. Higher supplemental level of seaweed was passible in foreign cookings. 2. Hae-Jo-Kang and Jeon-Byung were more excellent than Song-Pyun, wheat cake, Soo-Je-Bee and wheat noodle. Hae-Je-Kang was excellent in its quality even as high as 5% supplemental level. 3. The higher levels of supplementation were used the more sticky cooking products were obtained. Song-Pyun and wheat cake were palatable and lustrous in 2% supplementation level. 4. In drop cookie the higher levels of supplementation, the more crisp product was obtained, compared with other cookies. 5. Corn cake, thin rice gruel, rice gruel and potato Jeon-Byung were more excellent in their quality than potato Man-Doo and potato noodle. Corn cake, thin rice gruel and rice gruel were excellent even as high as 5% supplementation level. 6. In several cooking Porducts some seaweed-oder was perceived in case of 3% or more levels of supplementation. This may be much diminished by the use of proper condiments. D. Sanitary test It seems that there is no any heavy metals (Cd, Pb, As, Hg) problem in these seaweed Powders in case these Powders are used as supplements to any cereal flours E. Economical analysis The price of the seaweed powder is lower than those of other cereals and that may be more lowered when mass production of the seaweed powder is made in future. The supplement of the seaweed powder to any cereals is also economical with the criterion of animal growth rate. F. It is recommended that these seaweed powders should be developed and used as supplement to any cereal flours or used as other food material. By doing so, both saving of cereals and improvement of individual's nutrition will greatly be achieved. It is also recommended that the feeding experiment for men would be conducted in future.

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