• BMB Reports
• /
• v.43 no.2
• /
• pp.79-90
• /
• 2010

Mouse models are crucial for the functional annotation of human genome. Gene modification techniques including gene targeting and gene trap in mouse have provided powerful tools in the form of genetically engineered mice (GEM) for understanding the molecular pathogenesis of human diseases. Several international consortium and programs are under way to deliver mutations in every gene in mouse genome. The information from studying these GEM can be shared through international collaboration. However, there are many limitations in utility because not all human genes are knocked out in mouse and they are not yet phenotypically characterized by standardized ways which is required for sharing and evaluating data from GEM. The recent improvement in mouse genetics has now moved the bottleneck in mouse functional genomics from the production of GEM to the systematic mouse phenotype analysis of GEM. Enhanced, reproducible and comprehensive mouse phenotype analysis has thus emerged as a prerequisite for effectively engaging the phenotyping bottleneck. In this review, current information on systematic mouse phenotype analysis and an issue-oriented perspective will be provided.

• Journal of Oriental Neuropsychiatry
• /
• v.16 no.2
• /
• pp.251-257
• /
• 2005

In this case study, We treated a transcortical aphasia patient with herbal medicine, acupunture and language therapy. We assessed the progress of the patient with Western Aphaia Battery(K-WAB), Boston Naming Test(BNT) and analysed the patient's speech at the series of pictures. The score of K-WAB and K-BNT was improved, the rate of statement at the theme of the picture was improved and the neologistic and verbal paraphasia was reduced. We think that the analysing the speech of the patient at the series of pictures to evaluate the practical problem of the patient would be useful. Further study is necessary about the utility of this assessment tools.

• Journal of Microbiology and Biotechnology
• /
• v.19 no.3
• /
• pp.271-276
• /
• 2009

Functional gene studies in the cultivated white button mushroom Agaricus bisporus have been constrained by the absence of effective gene-silencing tools. Using two endogenous genes from A. bisporus, we have tested the utility of dsRNA hairpin constructs to mediate downregulation of specific genes. Hairpin constructs for genes encoding orotidine 5'-monophosphate decarboxylase (URA3) and carboxin resistance (CBX) were introduced into A. bisporus using Agrobacteriummediated transfection. Although predicted changes in phenotype were not observed in vitro, quantitative-PCR analyses indicated unambiguously that transcripts in several transformants were substantially reduced compared with the non-transformed controls. Interestingly, some hairpin transformants exhibited increased transcription of target genes. Our observations show that hairpin transgenic sequences can mediate downregulation of A. bisporus endogenous genes and that the technology has the potential to expedite functional genomics of the mushroom.

• Journal of Agricultural Extension & Community Development
• /
• v.4 no.1
• /
• pp.201-209
• /
• 1997

The objective of this study were 1) to review concepts of user information satisfaction (UIS), 2) to analyze tools for measuring user information satisfaction, 3) to identify factors affecting information satisfaction. The study was carried out by the analysis of literatures related to information system. The construct of UIS has been operationalized in many different ways. Several studies employed single-item rating scales; such scales have been criticized as unreliable. Single-item scales also provide little information as to what the user finds dissatisfying(satisfying) and are thus of limited utility outside a research setting. Multiple-item UIS measures have become increasingly common. Generally, they are of two types. The first focuses on the information system product. With such diverse names as "system acceptance", "output quality", and "appreciation", these scales focus on the content of the information system and the manner in which the information is presented. The second type of multiple-item scale includes the organizational support for developing and maintaining the system as well as the system product itself. This type of instrument contains items concerned with training, documentation, development procedures, systems maintenance, etc., as well as items related to system content. Thus it provides an indicator of the overall quality of information services provided by an information service function. Generally, UIS measures have not been carefully validated. Recently, however, several rigorous attempts have been made to develop valid and reliable UIS measure.

• Journal of Digital Contents Society
• /
• v.9 no.4
• /
• pp.779-784
• /
• 2008

An environment of information sharing is developed as a high-speed growth of internet infrastructure and a fast supply of digital imaging devices. Therefore, developing and supply various digital contents are needed. As occasion demands, the utility of editing tools with a simple image viewer, or programs to need technical education increased. One of subproblems is to take long time for editing images. In this paper, we propose a new method which is a method to edit images in a lump. This method has advantages compared with the previous searching engines in terns of performances and speed. Also, we propose the new approach for developing fusion graphic image contents as binding each advantages of the category. It reduces the production time and costs of contents by the proposed method.

• Korean Journal of Occupational Health Nursing
• /
• v.26 no.1
• /
• pp.1-9
• /
• 2017

Purpose: This study was conducted to evaluate the validity of the 'Enterprise Health Promotion Index', a tool for assessing health promotion activities of worksite developed by the Ministry of Employment and Labor and the Korea Occupational Safety and Health Agency. Methods: This is designed a methodological study that evaluates the validity of the Enterprise Health Promotion Index. For this study, a questionnaire survey was conducted for the workplace health professional and safety professional, and a total of 297 data were extracted as final data. We developed the informal health promotion needs and activity measurement tools. The correlation coefficient between the score of the enterprise health promotion index and the score of the informal health promotion tool was analyzed for the criterion validity evaluation. Results: The criterion validity of the Enterprise Health Promotion Index and activity index were supported by finding moderate (r=.597) and high (r=.783) correlation coefficient. However, the requirement index has low levels of criterion validity (r=.364). Conclusion: Based on these results, we suggest to improve the utility of the corporate health promotion index by developing user manual, active public relations, and providing briefing sessions.

• Molecules and Cells
• /
• v.39 no.9
• /
• pp.687-691
• /
• 2016

Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the convenient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.

• The Transactions of the Korean Institute of Electrical Engineers C
• /
• v.55 no.2
• /
• pp.82-87
• /
• 2006

Most of electric accidents are happened in the place where activities are frequent by common people who has low electric knowledge. Also, accidents are increased in the low voltage electric equipment. So, it is increased to spread the dispersion or non-utility power generation system. It means that ordinary times observation is required for electric equipments by common people. This paper is studied for the diagnosis method of electric equipment that common people can diagnose easily Voltage is generally and exactly measured in the electric and electronic field. We suggest new analysis tools using only electric voltage. It is called the Time-Energy analysis. The Time-Energy analysis is as following First, divide measured voltage waves into same parts, Second, conducted FFT(Fast Fourier Transform) transform for each divided period, Finally, each results are reassembled after graphic treatment. We compared Time-Energy analysis with discharge form by tracking tester. As the result, tracking discharge processes distinguished clearly.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.12
• /
• pp.1833-1839
• /
• 2004

Recently mass spectrometry and separation methods such as liquid chromatography have become major tools in the field of proteomics. In this report, we describe in detail our efforts to develop ultra-high pressure capillary reverse-phase liquid chromatography (cRPLC) and its online coupling to a mass spectrometer by a nanoelectrospray (nanoESI) interface. The RPLC system is constructed in house to deliver LC solvents at the pressure up to 20,000 psig, which is four times higher than conventional RPLC systems. The high operation pressure allows the efficient use of packed micro-capillary columns (50, 75 and 150 ${\mu}$m i.d., up to 1.5 m long). We will discuss the effect of column diameter on the sensitivity of cRPLC/MS/MS experiments and the utility of the developed technique for proteome analysis by its application in the analysis of proteome samples having different levels of complexity.

• Journal of Korean Academy of Nursing
• /
• v.40 no.6
• /
• pp.892-902
• /
• 2010

Purpose: This study was done to identify participation by home healthcare nurses in clinical decision making and factors influencing clinical decision making. Methods: A descriptive survey was used to collect data from 68 home healthcare nurses in 22 hospital-based home healthcare services in Korea. To investigate participation, the researcher developed 3 scenarios through interviews with 5 home healthcare nurses. A self-report questionnaire composed of tools for characteristics, factors of clinical decision making, and participation was used. Results: Participation was relatively high, but significantly lower in the design phase (F=3.51, p=.032). Competency in clinical decision making (r=.45, p<.001), perception of the decision maker role (r=.47, p<.001), and perception of the utility of clinical practice guidelines (r=.25, p=.043) were significantly correlated with participation. Competency in clinical decision making (Odds ratio [OR]=41.79, p=.007) and perception of the decision maker role (OR=15.09, p=.007) were significant factors predicting participation in clinical decision making by home healthcare nurses. Conclusion: In order to encourage participation in clinical decision making, education programs should be provided to home healthcare nurses. Official clinical practice guidelines should be used to support home healthcare nurses’ participation in clinical decision making in cases where they can identify and solve the patient health problems.